Panax vietnamensis Protects Mice Against Carbon Tetrachloride-Induced Hepatotoxicity without any Modification of CYP2E1 Gene Expression
Abstract In order to explore the effect of PANAX VIETNAMENSIS on carbon tetrachloride-induced hepatotoxicity, mice were pretreated for 7 days with either crude extract or total saponins. Crude extract and total saponins dramatically decreased carbon tetrachloride-induced increase of serum GSTα level...
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| Veröffentlicht in: | Planta medica 2000-12, Vol.66 (8), p.714-719 |
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| creator | Nguyen, T. D. Villard, P. H. Barlatier, A. Elsisi, A. E. Jouve, E. Duc, N. M. Sauze, C. Durand, A. Lacarelle, B. |
| description | Abstract
In order to explore the effect of PANAX VIETNAMENSIS on carbon tetrachloride-induced hepatotoxicity, mice were pretreated for 7 days with either crude extract or total saponins. Crude extract and total saponins dramatically decreased carbon tetrachloride-induced increase of serum GSTα level (- 50.0 %, - 49.5 % respectively). Serum AST level was significantly decreased only with total saponins (- 52.2 %) and ALT level was slightly modified. IN VITRO experiments shown that both preparations at high concentrations (> 2000 μg/ml) are able to inhibit CYP2E1 enzymatic activity in mouse and human microsomes. However, we did not observe any modification of CYP2E1 gene expression (enzymatic activity, protein and mRNA levels) in mice treated with either crude extract or total saponins. Taken together, these data demonstrated that PANAX VIETNAMENSIS could be used as an hepatoprotectant. However, the mechanism of action is not associated with CYP2E1 expression, as previously suggested IN VITRO in rat for total saponins from PANAX GINSENG. |
| doi_str_mv | 10.1055/s-2000-9603 |
| format | Article |
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In order to explore the effect of PANAX VIETNAMENSIS on carbon tetrachloride-induced hepatotoxicity, mice were pretreated for 7 days with either crude extract or total saponins. Crude extract and total saponins dramatically decreased carbon tetrachloride-induced increase of serum GSTα level (- 50.0 %, - 49.5 % respectively). Serum AST level was significantly decreased only with total saponins (- 52.2 %) and ALT level was slightly modified. IN VITRO experiments shown that both preparations at high concentrations (> 2000 μg/ml) are able to inhibit CYP2E1 enzymatic activity in mouse and human microsomes. However, we did not observe any modification of CYP2E1 gene expression (enzymatic activity, protein and mRNA levels) in mice treated with either crude extract or total saponins. Taken together, these data demonstrated that PANAX VIETNAMENSIS could be used as an hepatoprotectant. However, the mechanism of action is not associated with CYP2E1 expression, as previously suggested IN VITRO in rat for total saponins from PANAX GINSENG.</description><identifier>ISSN: 0032-0943</identifier><identifier>EISSN: 1439-0221</identifier><identifier>DOI: 10.1055/s-2000-9603</identifier><identifier>PMID: 11199127</identifier><identifier>CODEN: PLMEAA</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>Animals ; Biological and medical sciences ; Carbon Tetrachloride Poisoning - enzymology ; Carbon Tetrachloride Poisoning - prevention & control ; Chemical and Drug Induced Liver Injury - enzymology ; Chemical and Drug Induced Liver Injury - prevention & control ; Cytochrome P-450 CYP2E1 - genetics ; Gene Expression Regulation, Enzymologic ; General pharmacology ; Humans ; Male ; Medical sciences ; Mice ; Original Paper ; Panax - chemistry ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Plant Extracts - pharmacology ; Plants, Medicinal</subject><ispartof>Planta medica, 2000-12, Vol.66 (8), p.714-719</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c348t-22ba05861a2c074f4b59850c765d1a4c9bc747985b91b518d74a131d31f52d9c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2000-9603.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1055/s-2000-9603$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,776,780,3004,3005,27901,27902,54534,54535</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=819381$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11199127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nguyen, T. D.</creatorcontrib><creatorcontrib>Villard, P. H.</creatorcontrib><creatorcontrib>Barlatier, A.</creatorcontrib><creatorcontrib>Elsisi, A. E.</creatorcontrib><creatorcontrib>Jouve, E.</creatorcontrib><creatorcontrib>Duc, N. M.</creatorcontrib><creatorcontrib>Sauze, C.</creatorcontrib><creatorcontrib>Durand, A.</creatorcontrib><creatorcontrib>Lacarelle, B.</creatorcontrib><title>Panax vietnamensis Protects Mice Against Carbon Tetrachloride-Induced Hepatotoxicity without any Modification of CYP2E1 Gene Expression</title><title>Planta medica</title><addtitle>Planta Med</addtitle><description>Abstract
In order to explore the effect of PANAX VIETNAMENSIS on carbon tetrachloride-induced hepatotoxicity, mice were pretreated for 7 days with either crude extract or total saponins. Crude extract and total saponins dramatically decreased carbon tetrachloride-induced increase of serum GSTα level (- 50.0 %, - 49.5 % respectively). Serum AST level was significantly decreased only with total saponins (- 52.2 %) and ALT level was slightly modified. IN VITRO experiments shown that both preparations at high concentrations (> 2000 μg/ml) are able to inhibit CYP2E1 enzymatic activity in mouse and human microsomes. However, we did not observe any modification of CYP2E1 gene expression (enzymatic activity, protein and mRNA levels) in mice treated with either crude extract or total saponins. Taken together, these data demonstrated that PANAX VIETNAMENSIS could be used as an hepatoprotectant. However, the mechanism of action is not associated with CYP2E1 expression, as previously suggested IN VITRO in rat for total saponins from PANAX GINSENG.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Carbon Tetrachloride Poisoning - enzymology</subject><subject>Carbon Tetrachloride Poisoning - prevention & control</subject><subject>Chemical and Drug Induced Liver Injury - enzymology</subject><subject>Chemical and Drug Induced Liver Injury - prevention & control</subject><subject>Cytochrome P-450 CYP2E1 - genetics</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Original Paper</subject><subject>Panax - chemistry</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - pharmacology</subject><subject>Plants, Medicinal</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0E1vEzEQBmALgWhaOHFHlrgVLZ2xvdn1sYrSD6kVObQHTqtZ20tcJXZkO5D8Av42GyUqF06Wxs_MaF7GPiF8Q6jrq1wJAKj0FOQbNkEldQVC4Fs2AZCiAq3kGTvP-QUAlQZ4z84QUWsUzYT9WVCgHf_lXQm0diH7zBcpFmdK5o_eOH79k3zIhc8o9THwJ1cSmeUqJm9ddR_s1jjL79yGSixx540ve_7bl2XcFk5hzx-j9YM3VPzYHQc--7EQc-S3Ljg-322Sy3n8-cDeDbTK7uPpvWDPN_On2V318P32fnb9UBmp2lIJ0RPU7RRJGGjUoPpatzWYZlpbJGV0bxrVjKVeY19jaxtFKNFKHGphtZEX7Otxrkkx5-SGbpP8mtK-Q-gOcXa5O8TZHeIc9eej3mz7tbP_7Cm_EXw5AcqGVkOiYHx-dS1q2eKoLo-qLL1bu-4lblMYr_zvzr8674rw</recordid><startdate>20001201</startdate><enddate>20001201</enddate><creator>Nguyen, T. D.</creator><creator>Villard, P. H.</creator><creator>Barlatier, A.</creator><creator>Elsisi, A. E.</creator><creator>Jouve, E.</creator><creator>Duc, N. M.</creator><creator>Sauze, C.</creator><creator>Durand, A.</creator><creator>Lacarelle, B.</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20001201</creationdate><title>Panax vietnamensis Protects Mice Against Carbon Tetrachloride-Induced Hepatotoxicity without any Modification of CYP2E1 Gene Expression</title><author>Nguyen, T. D. ; Villard, P. H. ; Barlatier, A. ; Elsisi, A. E. ; Jouve, E. ; Duc, N. M. ; Sauze, C. ; Durand, A. ; Lacarelle, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c348t-22ba05861a2c074f4b59850c765d1a4c9bc747985b91b518d74a131d31f52d9c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Carbon Tetrachloride Poisoning - enzymology</topic><topic>Carbon Tetrachloride Poisoning - prevention & control</topic><topic>Chemical and Drug Induced Liver Injury - enzymology</topic><topic>Chemical and Drug Induced Liver Injury - prevention & control</topic><topic>Cytochrome P-450 CYP2E1 - genetics</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Original Paper</topic><topic>Panax - chemistry</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - pharmacology</topic><topic>Plants, Medicinal</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nguyen, T. D.</creatorcontrib><creatorcontrib>Villard, P. H.</creatorcontrib><creatorcontrib>Barlatier, A.</creatorcontrib><creatorcontrib>Elsisi, A. E.</creatorcontrib><creatorcontrib>Jouve, E.</creatorcontrib><creatorcontrib>Duc, N. M.</creatorcontrib><creatorcontrib>Sauze, C.</creatorcontrib><creatorcontrib>Durand, A.</creatorcontrib><creatorcontrib>Lacarelle, B.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nguyen, T. D.</au><au>Villard, P. H.</au><au>Barlatier, A.</au><au>Elsisi, A. E.</au><au>Jouve, E.</au><au>Duc, N. M.</au><au>Sauze, C.</au><au>Durand, A.</au><au>Lacarelle, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Panax vietnamensis Protects Mice Against Carbon Tetrachloride-Induced Hepatotoxicity without any Modification of CYP2E1 Gene Expression</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>2000-12-01</date><risdate>2000</risdate><volume>66</volume><issue>8</issue><spage>714</spage><epage>719</epage><pages>714-719</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract
In order to explore the effect of PANAX VIETNAMENSIS on carbon tetrachloride-induced hepatotoxicity, mice were pretreated for 7 days with either crude extract or total saponins. Crude extract and total saponins dramatically decreased carbon tetrachloride-induced increase of serum GSTα level (- 50.0 %, - 49.5 % respectively). Serum AST level was significantly decreased only with total saponins (- 52.2 %) and ALT level was slightly modified. IN VITRO experiments shown that both preparations at high concentrations (> 2000 μg/ml) are able to inhibit CYP2E1 enzymatic activity in mouse and human microsomes. However, we did not observe any modification of CYP2E1 gene expression (enzymatic activity, protein and mRNA levels) in mice treated with either crude extract or total saponins. Taken together, these data demonstrated that PANAX VIETNAMENSIS could be used as an hepatoprotectant. However, the mechanism of action is not associated with CYP2E1 expression, as previously suggested IN VITRO in rat for total saponins from PANAX GINSENG.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>11199127</pmid><doi>10.1055/s-2000-9603</doi><tpages>6</tpages></addata></record> |
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| ispartof | Planta medica, 2000-12, Vol.66 (8), p.714-719 |
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| subjects | Animals Biological and medical sciences Carbon Tetrachloride Poisoning - enzymology Carbon Tetrachloride Poisoning - prevention & control Chemical and Drug Induced Liver Injury - enzymology Chemical and Drug Induced Liver Injury - prevention & control Cytochrome P-450 CYP2E1 - genetics Gene Expression Regulation, Enzymologic General pharmacology Humans Male Medical sciences Mice Original Paper Panax - chemistry Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - pharmacology Plants, Medicinal |
| title | Panax vietnamensis Protects Mice Against Carbon Tetrachloride-Induced Hepatotoxicity without any Modification of CYP2E1 Gene Expression |
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