Inhibition of the Lethal and Myotoxic Activities of Crotalus durissus terrificus Venom by Tabernaemontana catharinensis: Identification of One of the Active Components
Abstract In Brazilian folk medicine, victims of bites by poisonous animals are usually treated with plant extracts derived from the diverse national flora. The chemical and pharmacological properties of most extracts were yet not investigated. In the rural community of Assis-SP, the root bark of TAB...
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| Veröffentlicht in: | Planta medica 2000-06, Vol.66 (5), p.424-428 |
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| creator | Batina, Maria de Fátima C. Cintra, Adelia C. O. Veronese, Elen L. G. Lavrador, Marco A. S. Giglio, José R. Pereira, Paulo S. Dias, Diones A. França, Suzelei C. Sampaio, Suely V. |
| description | Abstract
In Brazilian folk medicine, victims of bites by poisonous animals are usually treated with plant extracts derived from the diverse national flora. The chemical and pharmacological properties of most extracts were yet not investigated. In the rural community of Assis-SP, the root bark of TABERNAEMONTANA CATHARINENSIS (“leiteiro”, “cow milk”) is applied to the site of the snake bite and believed to neutralize the effect of the venom. We report here the ability of the lyophilized aqueous extract (AE) and of a pure compound obtained from the ethanolic extract of T. CATHARINENSIS to inhibit the lethal and myotoxic activities of C. D. TERRIFICUS (South American rattlesnake) venom. Doses of 10 mg AE/100 g, injected (I.M., rat) 20 s after injecting (I.M.) the venom and that of 2.5 mg AE/100 g, incubated for 1 h at 25 °C with the venom before injection (I.M.) were able to neutralize the lethal activity of 2LD
50
. These data indicate that T. CATHARINENSIS could be used as a source of a model molecule able to neutralize the lethality and myotoxicity induced by C. D. TERRIFICUS venom. Its ethanolic extract was then fractionated on a silica gel 60 chromatography column affording fractions A to F. Fraction A consisted basically of non-polar compounds, terpenes and sterols. Fraction D showed a pronounced antiophidian activity which was later correlated with the presence of the quaternary alkaloid 12-methoxy-4-methylvoachalotine in this fraction. This alkaloid was isolated and inhibited 100 % lethality when injected 20 s after 2 LD
50
at 1.7 mg/100 g. |
| doi_str_mv | 10.1055/s-2000-8577 |
| format | Article |
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In Brazilian folk medicine, victims of bites by poisonous animals are usually treated with plant extracts derived from the diverse national flora. The chemical and pharmacological properties of most extracts were yet not investigated. In the rural community of Assis-SP, the root bark of TABERNAEMONTANA CATHARINENSIS (“leiteiro”, “cow milk”) is applied to the site of the snake bite and believed to neutralize the effect of the venom. We report here the ability of the lyophilized aqueous extract (AE) and of a pure compound obtained from the ethanolic extract of T. CATHARINENSIS to inhibit the lethal and myotoxic activities of C. D. TERRIFICUS (South American rattlesnake) venom. Doses of 10 mg AE/100 g, injected (I.M., rat) 20 s after injecting (I.M.) the venom and that of 2.5 mg AE/100 g, incubated for 1 h at 25 °C with the venom before injection (I.M.) were able to neutralize the lethal activity of 2LD
50
. These data indicate that T. CATHARINENSIS could be used as a source of a model molecule able to neutralize the lethality and myotoxicity induced by C. D. TERRIFICUS venom. Its ethanolic extract was then fractionated on a silica gel 60 chromatography column affording fractions A to F. Fraction A consisted basically of non-polar compounds, terpenes and sterols. Fraction D showed a pronounced antiophidian activity which was later correlated with the presence of the quaternary alkaloid 12-methoxy-4-methylvoachalotine in this fraction. This alkaloid was isolated and inhibited 100 % lethality when injected 20 s after 2 LD
50
at 1.7 mg/100 g.</description><identifier>ISSN: 0032-0943</identifier><identifier>EISSN: 1439-0221</identifier><identifier>DOI: 10.1055/s-2000-8577</identifier><identifier>PMID: 10909261</identifier><identifier>CODEN: PLMEAA</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>Alkaloids - chemistry ; Alkaloids - isolation & purification ; Alkaloids - pharmacology ; Animals ; Antidotes - chemistry ; Antidotes - isolation & purification ; Antidotes - pharmacology ; Biological and medical sciences ; Carbazoles - chemistry ; Carbazoles - isolation & purification ; Carbazoles - pharmacology ; Chromatography, Thin Layer ; Creatine Kinase - blood ; Crotalid Venoms - antagonists & inhibitors ; Crotalid Venoms - poisoning ; General pharmacology ; Lethal Dose 50 ; Male ; Medical sciences ; Muscle, Skeletal - drug effects ; Muscle, Skeletal - enzymology ; Original Paper ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Plant Extracts - pharmacology ; Plant Roots - chemistry ; Plants, Medicinal - chemistry ; Poisoning - drug therapy ; Poisoning - mortality ; Rats ; Rats, Wistar ; Trees - chemistry</subject><ispartof>Planta medica, 2000-06, Vol.66 (5), p.424-428</ispartof><rights>Georg Thieme Verlag Stuttgart · New York</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-4e6ae12ea8a11467d0ef55344d6eb851c45c6edb2ee9aa22c10da0abf2baa7fe3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2000-8577.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><linktohtml>$$Uhttps://www.thieme-connect.de/products/ejournals/html/10.1055/s-2000-8577$$EHTML$$P50$$Gthieme$$H</linktohtml><link.rule.ids>314,780,784,3017,3018,27924,27925,54559,54560</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1405599$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10909261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Batina, Maria de Fátima C.</creatorcontrib><creatorcontrib>Cintra, Adelia C. O.</creatorcontrib><creatorcontrib>Veronese, Elen L. G.</creatorcontrib><creatorcontrib>Lavrador, Marco A. S.</creatorcontrib><creatorcontrib>Giglio, José R.</creatorcontrib><creatorcontrib>Pereira, Paulo S.</creatorcontrib><creatorcontrib>Dias, Diones A.</creatorcontrib><creatorcontrib>França, Suzelei C.</creatorcontrib><creatorcontrib>Sampaio, Suely V.</creatorcontrib><title>Inhibition of the Lethal and Myotoxic Activities of Crotalus durissus terrificus Venom by Tabernaemontana catharinensis: Identification of One of the Active Components</title><title>Planta medica</title><addtitle>Planta Med</addtitle><description>Abstract
In Brazilian folk medicine, victims of bites by poisonous animals are usually treated with plant extracts derived from the diverse national flora. The chemical and pharmacological properties of most extracts were yet not investigated. In the rural community of Assis-SP, the root bark of TABERNAEMONTANA CATHARINENSIS (“leiteiro”, “cow milk”) is applied to the site of the snake bite and believed to neutralize the effect of the venom. We report here the ability of the lyophilized aqueous extract (AE) and of a pure compound obtained from the ethanolic extract of T. CATHARINENSIS to inhibit the lethal and myotoxic activities of C. D. TERRIFICUS (South American rattlesnake) venom. Doses of 10 mg AE/100 g, injected (I.M., rat) 20 s after injecting (I.M.) the venom and that of 2.5 mg AE/100 g, incubated for 1 h at 25 °C with the venom before injection (I.M.) were able to neutralize the lethal activity of 2LD
50
. These data indicate that T. CATHARINENSIS could be used as a source of a model molecule able to neutralize the lethality and myotoxicity induced by C. D. TERRIFICUS venom. Its ethanolic extract was then fractionated on a silica gel 60 chromatography column affording fractions A to F. Fraction A consisted basically of non-polar compounds, terpenes and sterols. Fraction D showed a pronounced antiophidian activity which was later correlated with the presence of the quaternary alkaloid 12-methoxy-4-methylvoachalotine in this fraction. This alkaloid was isolated and inhibited 100 % lethality when injected 20 s after 2 LD
50
at 1.7 mg/100 g.</description><subject>Alkaloids - chemistry</subject><subject>Alkaloids - isolation & purification</subject><subject>Alkaloids - pharmacology</subject><subject>Animals</subject><subject>Antidotes - chemistry</subject><subject>Antidotes - isolation & purification</subject><subject>Antidotes - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Carbazoles - chemistry</subject><subject>Carbazoles - isolation & purification</subject><subject>Carbazoles - pharmacology</subject><subject>Chromatography, Thin Layer</subject><subject>Creatine Kinase - blood</subject><subject>Crotalid Venoms - antagonists & inhibitors</subject><subject>Crotalid Venoms - poisoning</subject><subject>General pharmacology</subject><subject>Lethal Dose 50</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Muscle, Skeletal - drug effects</subject><subject>Muscle, Skeletal - enzymology</subject><subject>Original Paper</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - pharmacology</subject><subject>Plant Roots - chemistry</subject><subject>Plants, Medicinal - chemistry</subject><subject>Poisoning - drug therapy</subject><subject>Poisoning - mortality</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Trees - chemistry</subject><issn>0032-0943</issn><issn>1439-0221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkMFO3DAQhi0EKlvaE3fkA6dWgbHjZNfc0AralbbiAr1GE2eiNUrsle1F7BP1Net0Qe2hp5nDN_8_-hg7F3AloKquYyEBoFhU8_kRmwlV6gKkFMdsBlDKArQqT9nHGJ8BhNIAH9ipAA1a1mLGfq3cxrY2We-473naEF9T2uDA0XX8x94n_2oNvzXJvmSK4kQtg0847CLvdsHGmJdEIdjemrz-JOdH3u75I7YUHNLoXUKH3GDODdaRizbe8FVHLk03-F7-4Oj9hz99xJd-3Pp8kOIndtLjEOnz2zxjT_d3j8vvxfrh22p5uy5MqXQqFNVIQhIuUAhVzzugvqpKpbqa2kUljKpMTV0riTSilEZAh4BtL1vEeU_lGft6yDXBxxiob7bBjhj2jYBm0t3EZtLdTLozfXGgt7t2pO4f9uA3A5dvAEaDQx_QGRv_cionap2xLwcsbSyN1Dz7XTY3xP-W_gYY05nY</recordid><startdate>20000601</startdate><enddate>20000601</enddate><creator>Batina, Maria de Fátima C.</creator><creator>Cintra, Adelia C. O.</creator><creator>Veronese, Elen L. G.</creator><creator>Lavrador, Marco A. S.</creator><creator>Giglio, José R.</creator><creator>Pereira, Paulo S.</creator><creator>Dias, Diones A.</creator><creator>França, Suzelei C.</creator><creator>Sampaio, Suely V.</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20000601</creationdate><title>Inhibition of the Lethal and Myotoxic Activities of Crotalus durissus terrificus Venom by Tabernaemontana catharinensis: Identification of One of the Active Components</title><author>Batina, Maria de Fátima C. ; Cintra, Adelia C. O. ; Veronese, Elen L. G. ; Lavrador, Marco A. S. ; Giglio, José R. ; Pereira, Paulo S. ; Dias, Diones A. ; França, Suzelei C. ; Sampaio, Suely V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-4e6ae12ea8a11467d0ef55344d6eb851c45c6edb2ee9aa22c10da0abf2baa7fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Alkaloids - chemistry</topic><topic>Alkaloids - isolation & purification</topic><topic>Alkaloids - pharmacology</topic><topic>Animals</topic><topic>Antidotes - chemistry</topic><topic>Antidotes - isolation & purification</topic><topic>Antidotes - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Carbazoles - chemistry</topic><topic>Carbazoles - isolation & purification</topic><topic>Carbazoles - pharmacology</topic><topic>Chromatography, Thin Layer</topic><topic>Creatine Kinase - blood</topic><topic>Crotalid Venoms - antagonists & inhibitors</topic><topic>Crotalid Venoms - poisoning</topic><topic>General pharmacology</topic><topic>Lethal Dose 50</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Muscle, Skeletal - drug effects</topic><topic>Muscle, Skeletal - enzymology</topic><topic>Original Paper</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - pharmacology</topic><topic>Plant Roots - chemistry</topic><topic>Plants, Medicinal - chemistry</topic><topic>Poisoning - drug therapy</topic><topic>Poisoning - mortality</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Trees - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Batina, Maria de Fátima C.</creatorcontrib><creatorcontrib>Cintra, Adelia C. O.</creatorcontrib><creatorcontrib>Veronese, Elen L. G.</creatorcontrib><creatorcontrib>Lavrador, Marco A. S.</creatorcontrib><creatorcontrib>Giglio, José R.</creatorcontrib><creatorcontrib>Pereira, Paulo S.</creatorcontrib><creatorcontrib>Dias, Diones A.</creatorcontrib><creatorcontrib>França, Suzelei C.</creatorcontrib><creatorcontrib>Sampaio, Suely V.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Planta medica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Batina, Maria de Fátima C.</au><au>Cintra, Adelia C. O.</au><au>Veronese, Elen L. G.</au><au>Lavrador, Marco A. S.</au><au>Giglio, José R.</au><au>Pereira, Paulo S.</au><au>Dias, Diones A.</au><au>França, Suzelei C.</au><au>Sampaio, Suely V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of the Lethal and Myotoxic Activities of Crotalus durissus terrificus Venom by Tabernaemontana catharinensis: Identification of One of the Active Components</atitle><jtitle>Planta medica</jtitle><addtitle>Planta Med</addtitle><date>2000-06-01</date><risdate>2000</risdate><volume>66</volume><issue>5</issue><spage>424</spage><epage>428</epage><pages>424-428</pages><issn>0032-0943</issn><eissn>1439-0221</eissn><coden>PLMEAA</coden><abstract>Abstract
In Brazilian folk medicine, victims of bites by poisonous animals are usually treated with plant extracts derived from the diverse national flora. The chemical and pharmacological properties of most extracts were yet not investigated. In the rural community of Assis-SP, the root bark of TABERNAEMONTANA CATHARINENSIS (“leiteiro”, “cow milk”) is applied to the site of the snake bite and believed to neutralize the effect of the venom. We report here the ability of the lyophilized aqueous extract (AE) and of a pure compound obtained from the ethanolic extract of T. CATHARINENSIS to inhibit the lethal and myotoxic activities of C. D. TERRIFICUS (South American rattlesnake) venom. Doses of 10 mg AE/100 g, injected (I.M., rat) 20 s after injecting (I.M.) the venom and that of 2.5 mg AE/100 g, incubated for 1 h at 25 °C with the venom before injection (I.M.) were able to neutralize the lethal activity of 2LD
50
. These data indicate that T. CATHARINENSIS could be used as a source of a model molecule able to neutralize the lethality and myotoxicity induced by C. D. TERRIFICUS venom. Its ethanolic extract was then fractionated on a silica gel 60 chromatography column affording fractions A to F. Fraction A consisted basically of non-polar compounds, terpenes and sterols. Fraction D showed a pronounced antiophidian activity which was later correlated with the presence of the quaternary alkaloid 12-methoxy-4-methylvoachalotine in this fraction. This alkaloid was isolated and inhibited 100 % lethality when injected 20 s after 2 LD
50
at 1.7 mg/100 g.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>10909261</pmid><doi>10.1055/s-2000-8577</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Planta medica, 2000-06, Vol.66 (5), p.424-428 |
| issn | 0032-0943 1439-0221 |
| language | eng |
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| source | MEDLINE; Thieme Connect Journals |
| subjects | Alkaloids - chemistry Alkaloids - isolation & purification Alkaloids - pharmacology Animals Antidotes - chemistry Antidotes - isolation & purification Antidotes - pharmacology Biological and medical sciences Carbazoles - chemistry Carbazoles - isolation & purification Carbazoles - pharmacology Chromatography, Thin Layer Creatine Kinase - blood Crotalid Venoms - antagonists & inhibitors Crotalid Venoms - poisoning General pharmacology Lethal Dose 50 Male Medical sciences Muscle, Skeletal - drug effects Muscle, Skeletal - enzymology Original Paper Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - pharmacology Plant Roots - chemistry Plants, Medicinal - chemistry Poisoning - drug therapy Poisoning - mortality Rats Rats, Wistar Trees - chemistry |
| title | Inhibition of the Lethal and Myotoxic Activities of Crotalus durissus terrificus Venom by Tabernaemontana catharinensis: Identification of One of the Active Components |
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