Immunohistochemical expression of MTA1 MTA3, CRIP1, Caspase 8, TUNEL and M30 in placental tissue of normal and preeclamptic pregnancies

Introduction: Preeclampsia is a serious obstetric problem and is characterized by significant rates of maternal and neonatal morbidity and mortality and its cause remains virtually unknown despite the several theories that have been put forward. Objective of the study: The investigation of 6 placental tissue markers and the comparison of their expression in placentas between preeclamptic women and women who did not develop preeclampsia. Because of the correlation of abnormal placentation which occurs in preeclampsia with cell apoptosis, we studied the markers TUNEL, C8 and M30, and because of the association of abnormal placentation with the process of tumorgenesis we studied the markers MTA1, MTA3 and CRIP1. Materials: The materials for the execution of this experiment were fifty-one (51) samples of placental tissue. Twenty four (24) of them were derived from tissues of preeclamptic pregnancies while the rest twenty-seven (27) were obtained from normal pregnancies and were used as controls. Experimental procedure: The samples after the procedure of immunohistochemistry were studied by electron microscopy, and their expression was scored according to the German semi-quantitative scoring system considering the staining intensity and extent (PPSI).