A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells
The two-photon absorption properties of a pyrene-pyridinium dye ( 1 ) were studied for potential application in two-photon spectroscopy. When probe 1 was used in cellular two-photon fluorescence microscopy imaging, it allowed the visualization of nuclei in live cells with a relatively low probe conc...
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| Veröffentlicht in: | Photochemical & photobiological sciences 2020-09, Vol.19 (9), p.1152-1159 |
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| container_title | Photochemical & photobiological sciences |
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| creator | Abeywickrama, Chathura S Wijesinghe, Kaveesha J Plescia, Caroline B Fisher, Lloyd S Goodson III, Theodore Stahelin, Robert V Pang, Yi |
| description | The two-photon absorption properties of a pyrene-pyridinium dye (
1
) were studied for potential application in two-photon spectroscopy. When probe
1
was used in cellular two-photon fluorescence microscopy imaging, it allowed the visualization of nuclei in live cells with a relatively low probe concentration (such as 1 μM). Spectroscopic evidence further revealed that probe
1
interacted with DNA as an intercalator. The proposed DNA intercalation properties of probe
1
were consistent with the experimental findings that suggested that the observed nucleus staining ability is dependent on the substituents on the pyridinium fragment of the probe.
The two-photon absorption properties of a pyrene-pyridinium dye (
1
) were studied for potential application in two-photon spectroscopy. |
| doi_str_mv | 10.1039/d0pp00107d |
| format | Article |
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1
) were studied for potential application in two-photon spectroscopy. When probe
1
was used in cellular two-photon fluorescence microscopy imaging, it allowed the visualization of nuclei in live cells with a relatively low probe concentration (such as 1 μM). Spectroscopic evidence further revealed that probe
1
interacted with DNA as an intercalator. The proposed DNA intercalation properties of probe
1
were consistent with the experimental findings that suggested that the observed nucleus staining ability is dependent on the substituents on the pyridinium fragment of the probe.
The two-photon absorption properties of a pyrene-pyridinium dye (
1
) were studied for potential application in two-photon spectroscopy.</description><identifier>ISSN: 1474-905X</identifier><identifier>EISSN: 1474-9092</identifier><identifier>DOI: 10.1039/d0pp00107d</identifier><identifier>PMID: 32639494</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animals ; Biochemistry ; Biomaterials ; Cattle ; Cell Nucleus - chemistry ; Cell Survival ; Cells, Cultured ; Chemistry ; Chlorocebus aethiops ; COS Cells ; Deoxyribonucleic acid ; DNA ; DNA - chemistry ; Fluorescence ; Fluorescence microscopy ; Fluorescent Dyes - chemistry ; Fluorescent indicators ; Microscopy, Fluorescence ; Molecular Structure ; Nuclei ; Nuclei (cytology) ; Photon absorption ; Photons ; Physical Chemistry ; Plant Sciences ; Pyrene ; Pyrenes - chemistry ; Pyridinium ; Pyridinium Compounds - chemistry ; Spectroscopy</subject><ispartof>Photochemical & photobiological sciences, 2020-09, Vol.19 (9), p.1152-1159</ispartof><rights>The Royal Society of Chemistry and Owner Societies 2020</rights><rights>Copyright Royal Society of Chemistry 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-d6429ddf41cf0b50c6909d23ce0605e3f16b80c3071237ee11cb124b480bcbe93</citedby><cites>FETCH-LOGICAL-c472t-d6429ddf41cf0b50c6909d23ce0605e3f16b80c3071237ee11cb124b480bcbe93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1039/d0pp00107d$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1039/d0pp00107d$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32639494$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abeywickrama, Chathura S</creatorcontrib><creatorcontrib>Wijesinghe, Kaveesha J</creatorcontrib><creatorcontrib>Plescia, Caroline B</creatorcontrib><creatorcontrib>Fisher, Lloyd S</creatorcontrib><creatorcontrib>Goodson III, Theodore</creatorcontrib><creatorcontrib>Stahelin, Robert V</creatorcontrib><creatorcontrib>Pang, Yi</creatorcontrib><title>A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells</title><title>Photochemical & photobiological sciences</title><addtitle>Photochem Photobiol Sci</addtitle><addtitle>Photochem Photobiol Sci</addtitle><description>The two-photon absorption properties of a pyrene-pyridinium dye (
1
) were studied for potential application in two-photon spectroscopy. When probe
1
was used in cellular two-photon fluorescence microscopy imaging, it allowed the visualization of nuclei in live cells with a relatively low probe concentration (such as 1 μM). Spectroscopic evidence further revealed that probe
1
interacted with DNA as an intercalator. The proposed DNA intercalation properties of probe
1
were consistent with the experimental findings that suggested that the observed nucleus staining ability is dependent on the substituents on the pyridinium fragment of the probe.
The two-photon absorption properties of a pyrene-pyridinium dye (
1
) were studied for potential application in two-photon spectroscopy.</description><subject>Animals</subject><subject>Biochemistry</subject><subject>Biomaterials</subject><subject>Cattle</subject><subject>Cell Nucleus - chemistry</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Chemistry</subject><subject>Chlorocebus aethiops</subject><subject>COS Cells</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>Fluorescence</subject><subject>Fluorescence microscopy</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Fluorescent indicators</subject><subject>Microscopy, Fluorescence</subject><subject>Molecular Structure</subject><subject>Nuclei</subject><subject>Nuclei (cytology)</subject><subject>Photon absorption</subject><subject>Photons</subject><subject>Physical Chemistry</subject><subject>Plant Sciences</subject><subject>Pyrene</subject><subject>Pyrenes - chemistry</subject><subject>Pyridinium</subject><subject>Pyridinium Compounds - chemistry</subject><subject>Spectroscopy</subject><issn>1474-905X</issn><issn>1474-9092</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90NtLwzAUBvAgipvTF9-ViC-iVHNbL49jeIOBL4q-lSY51Y6uqUk7nX-9GZ0biPiUA-fHl4-D0CEll5Tw5EqTuiaEkkhvoT4VkQgSkrDt9Tx86aE956beDEUY7aIeZyFPRCL66HmE64WFCgKZOdC4-TBB_WYaU2H4VEWTyRJwXrbGglNQNbi2RgJuDJ4Xrs3K4gtw1aoSClxUuCzmgBWUpdtHO3lWOjhYvQP0dHP9OL4LJg-39-PRJFAiYk2gQ8ESrXNBVU7kkKjQV9eMKyAhGQLPaShjojiJKOMRAKVKUiakiIlUEhI-QGddru_13oJr0lnhlg2yCkzrUiYYFSFhPPT09BedmtZWvp1XgsWUi3ipzjulrHHOQp7WtphldpFSki7PnW7O7fHxKrKVM9Br-nNfDy464PyqegW7-fPPuJNOW6fWWZt9Wuvcm6P_DP8GBQ-eLg</recordid><startdate>20200909</startdate><enddate>20200909</enddate><creator>Abeywickrama, Chathura S</creator><creator>Wijesinghe, Kaveesha J</creator><creator>Plescia, Caroline B</creator><creator>Fisher, Lloyd S</creator><creator>Goodson III, Theodore</creator><creator>Stahelin, Robert V</creator><creator>Pang, Yi</creator><general>Springer International Publishing</general><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20200909</creationdate><title>A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells</title><author>Abeywickrama, Chathura S ; Wijesinghe, Kaveesha J ; Plescia, Caroline B ; Fisher, Lloyd S ; Goodson III, Theodore ; Stahelin, Robert V ; Pang, Yi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-d6429ddf41cf0b50c6909d23ce0605e3f16b80c3071237ee11cb124b480bcbe93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Biochemistry</topic><topic>Biomaterials</topic><topic>Cattle</topic><topic>Cell Nucleus - chemistry</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Chemistry</topic><topic>Chlorocebus aethiops</topic><topic>COS Cells</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - chemistry</topic><topic>Fluorescence</topic><topic>Fluorescence microscopy</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Fluorescent indicators</topic><topic>Microscopy, Fluorescence</topic><topic>Molecular Structure</topic><topic>Nuclei</topic><topic>Nuclei (cytology)</topic><topic>Photon absorption</topic><topic>Photons</topic><topic>Physical Chemistry</topic><topic>Plant Sciences</topic><topic>Pyrene</topic><topic>Pyrenes - chemistry</topic><topic>Pyridinium</topic><topic>Pyridinium Compounds - chemistry</topic><topic>Spectroscopy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abeywickrama, Chathura S</creatorcontrib><creatorcontrib>Wijesinghe, Kaveesha J</creatorcontrib><creatorcontrib>Plescia, Caroline B</creatorcontrib><creatorcontrib>Fisher, Lloyd S</creatorcontrib><creatorcontrib>Goodson III, Theodore</creatorcontrib><creatorcontrib>Stahelin, Robert V</creatorcontrib><creatorcontrib>Pang, Yi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Photochemical & photobiological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abeywickrama, Chathura S</au><au>Wijesinghe, Kaveesha J</au><au>Plescia, Caroline B</au><au>Fisher, Lloyd S</au><au>Goodson III, Theodore</au><au>Stahelin, Robert V</au><au>Pang, Yi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells</atitle><jtitle>Photochemical & photobiological sciences</jtitle><stitle>Photochem Photobiol Sci</stitle><addtitle>Photochem Photobiol Sci</addtitle><date>2020-09-09</date><risdate>2020</risdate><volume>19</volume><issue>9</issue><spage>1152</spage><epage>1159</epage><pages>1152-1159</pages><issn>1474-905X</issn><eissn>1474-9092</eissn><abstract>The two-photon absorption properties of a pyrene-pyridinium dye (
1
) were studied for potential application in two-photon spectroscopy. When probe
1
was used in cellular two-photon fluorescence microscopy imaging, it allowed the visualization of nuclei in live cells with a relatively low probe concentration (such as 1 μM). Spectroscopic evidence further revealed that probe
1
interacted with DNA as an intercalator. The proposed DNA intercalation properties of probe
1
were consistent with the experimental findings that suggested that the observed nucleus staining ability is dependent on the substituents on the pyridinium fragment of the probe.
The two-photon absorption properties of a pyrene-pyridinium dye (
1
) were studied for potential application in two-photon spectroscopy.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>32639494</pmid><doi>10.1039/d0pp00107d</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1474-905X |
| ispartof | Photochemical & photobiological sciences, 2020-09, Vol.19 (9), p.1152-1159 |
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| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Biochemistry Biomaterials Cattle Cell Nucleus - chemistry Cell Survival Cells, Cultured Chemistry Chlorocebus aethiops COS Cells Deoxyribonucleic acid DNA DNA - chemistry Fluorescence Fluorescence microscopy Fluorescent Dyes - chemistry Fluorescent indicators Microscopy, Fluorescence Molecular Structure Nuclei Nuclei (cytology) Photon absorption Photons Physical Chemistry Plant Sciences Pyrene Pyrenes - chemistry Pyridinium Pyridinium Compounds - chemistry Spectroscopy |
| title | A pyrene-based two-photon excitable fluorescent probe to visualize nuclei in live cells |
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