Butane and propane oxidation by engineered cytochrome P450cam
The haem monooxygenase cytochrome P450cam has been engineered to oxidise the gaseous alkanes butane and propane to butan-2-ol and propan-2-ol, respectively, by the use of bulky amino acid substitutions to reduce the volume of the substrate pocket and thus improve the enzyme-substrate fit: the F87W/Y...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2002-03 (5), p.490-491 |
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| container_title | Chemical communications (Cambridge, England) |
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| creator | Bell, Stephen G Stevenson, Julie-Anne Boyd, Helen D Campbell, Sophie Riddle, Austin D Orton, Erica L Wong, Luet-Lok |
| description | The haem monooxygenase cytochrome P450cam has been engineered to oxidise the gaseous alkanes butane and propane to butan-2-ol and propan-2-ol, respectively, by the use of bulky amino acid substitutions to reduce the volume of the substrate pocket and thus improve the enzyme-substrate fit: the F87W/Y96F/T101L/V247L mutant oxidizes butane with a turnover rate of 750 min-1 and 95% yield based on NADH consumed while the wild-type enzyme has an activity of 0.4 min-1 with 4% yield. |
| doi_str_mv | 10.1039/b110957j |
| format | Article |
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| ispartof | Chemical communications (Cambridge, England), 2002-03 (5), p.490-491 |
| issn | 1359-7345 1364-548X |
| language | eng |
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| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Binding Sites - genetics Butanes - metabolism Butanols Camphor 5-Monooxygenase - genetics Camphor 5-Monooxygenase - metabolism Kinetics Mutagenesis, Site-Directed Oxidation-Reduction Propane - metabolism Propanols Protein Engineering Pseudomonas putida - enzymology |
| title | Butane and propane oxidation by engineered cytochrome P450cam |
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