Development of an aptasensor for dibutyl phthalate detection and the elucidation of assay inhibition factors
We developed a fluorescence aptasensor (hereafter 'SG-aptasensor') using SYBR Green I, a newly truncated 20-mer aptamer, and probe DNA to detect dibutyl phthalate (DBP). The detection range of DBP was 0.1-100 ng L −1 with 0.08 ng L −1 as the limit of detection. To adapt the assay to enviro...
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| Veröffentlicht in: | RSC advances 2024-06, Vol.14 (29), p.2585-2594 |
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| creator | Song, Hyerin Lim, Hyun Jeong Son, Ahjeong |
| description | We developed a fluorescence aptasensor (hereafter 'SG-aptasensor') using SYBR Green I, a newly truncated 20-mer aptamer, and probe DNA to detect dibutyl phthalate (DBP). The detection range of DBP was 0.1-100 ng L
−1
with 0.08 ng L
−1
as the limit of detection. To adapt the assay to environmental samples in the near future, possible inhibition factors (experimental and environmental) have been tested and reported. The experimental inhibitors included the incubation time, temperature, pH, and ionic strength. Consequently, temperature (2-25 °C) and pH (7.0-9.0) ranges did not significantly inhibit the assay. The incubation time required for sufficient reaction was at least 4 h, and a relative humidity |
| doi_str_mv | 10.1039/d4ra03045a |
| format | Article |
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−1
with 0.08 ng L
−1
as the limit of detection. To adapt the assay to environmental samples in the near future, possible inhibition factors (experimental and environmental) have been tested and reported. The experimental inhibitors included the incubation time, temperature, pH, and ionic strength. Consequently, temperature (2-25 °C) and pH (7.0-9.0) ranges did not significantly inhibit the assay. The incubation time required for sufficient reaction was at least 4 h, and a relative humidity <20% may have induced fluorescence quenching. Tris-HCl-based incubation buffer with excess ionic strength (more than 0.2 M NaCl) demonstrated an abnormal increase in fluorescence. Environmental inhibitors including cations (Mg
2+
, Ca
2+
, and Cu
2+
) and humic acids were tested. The fluorescence signal was significantly reduced (∼99%) by 100 mM Cu
2+
compared to that by 0 mM Cu
2+
. In contrast, the reduction in fluorescence signal was marginal (<15%) when Mg
2+
or Ca
2+
ions were present. Inhibition of the assay was observed (∼28%) in the presence of 100 mg L
−1
humic acids.
DBP detection using SG-aptasensor and the elucidation of inhibition effects.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/d4ra03045a</identifier><identifier>PMID: 38946763</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Assaying ; Calcium ions ; Copper ; Dibutyl phthalate ; Fluorescence ; Humic acids ; Inhibitors ; Relative humidity</subject><ispartof>RSC advances, 2024-06, Vol.14 (29), p.2585-2594</ispartof><rights>This journal is © The Royal Society of Chemistry.</rights><rights>Copyright Royal Society of Chemistry 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c262t-c1411b3daeec20bd77ff4c54a94f16ee15960e021d9e3f262ea8a3d6f876a2a83</cites><orcidid>0000-0001-6210-0769</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38946763$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Song, Hyerin</creatorcontrib><creatorcontrib>Lim, Hyun Jeong</creatorcontrib><creatorcontrib>Son, Ahjeong</creatorcontrib><title>Development of an aptasensor for dibutyl phthalate detection and the elucidation of assay inhibition factors</title><title>RSC advances</title><addtitle>RSC Adv</addtitle><description>We developed a fluorescence aptasensor (hereafter 'SG-aptasensor') using SYBR Green I, a newly truncated 20-mer aptamer, and probe DNA to detect dibutyl phthalate (DBP). The detection range of DBP was 0.1-100 ng L
−1
with 0.08 ng L
−1
as the limit of detection. To adapt the assay to environmental samples in the near future, possible inhibition factors (experimental and environmental) have been tested and reported. The experimental inhibitors included the incubation time, temperature, pH, and ionic strength. Consequently, temperature (2-25 °C) and pH (7.0-9.0) ranges did not significantly inhibit the assay. The incubation time required for sufficient reaction was at least 4 h, and a relative humidity <20% may have induced fluorescence quenching. Tris-HCl-based incubation buffer with excess ionic strength (more than 0.2 M NaCl) demonstrated an abnormal increase in fluorescence. Environmental inhibitors including cations (Mg
2+
, Ca
2+
, and Cu
2+
) and humic acids were tested. The fluorescence signal was significantly reduced (∼99%) by 100 mM Cu
2+
compared to that by 0 mM Cu
2+
. In contrast, the reduction in fluorescence signal was marginal (<15%) when Mg
2+
or Ca
2+
ions were present. Inhibition of the assay was observed (∼28%) in the presence of 100 mg L
−1
humic acids.
DBP detection using SG-aptasensor and the elucidation of inhibition effects.</description><subject>Assaying</subject><subject>Calcium ions</subject><subject>Copper</subject><subject>Dibutyl phthalate</subject><subject>Fluorescence</subject><subject>Humic acids</subject><subject>Inhibitors</subject><subject>Relative humidity</subject><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpd0VtLBCEUB3CJoqJ66b0QeolgS0fH2XlctissBFHPwxk9shOz46ROsN8-99KFBFGOP_-Ih5BTzq45E-WNkR6YYDKHHXKYMalGGVPl7p_9ATkJ4Z2loXKeKb5PDsS4lKpQ4pC0t_iJresX2EXqLIWOQh8hYBecpzZN09RDXLa0n8c5tBCRGoyoY-MS7QyNc6TYDroxsK6tQkKAJW26eVM365oFHZ0Px2TPQhvwZLsekbf7u9fp42j2_PA0ncxGOlNZHGkuOa-FAUSdsdoUhbVS5xJKablC5HmpGLKMmxKFTVcQxiCMsuNCQQZjcUQuN7m9dx8DhlgtmqCxbaFDN4RKsEJyIWVeJnrxj767wXfpdStVFKKUskjqaqO0dyF4tFXvmwX4ZcVZtWpDdStfJus2TBI-30YO9QLND_3-9ATONsAH_XP620fxBVKMjUc</recordid><startdate>20240627</startdate><enddate>20240627</enddate><creator>Song, Hyerin</creator><creator>Lim, Hyun Jeong</creator><creator>Son, Ahjeong</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6210-0769</orcidid></search><sort><creationdate>20240627</creationdate><title>Development of an aptasensor for dibutyl phthalate detection and the elucidation of assay inhibition factors</title><author>Song, Hyerin ; Lim, Hyun Jeong ; Son, Ahjeong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c262t-c1411b3daeec20bd77ff4c54a94f16ee15960e021d9e3f262ea8a3d6f876a2a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Assaying</topic><topic>Calcium ions</topic><topic>Copper</topic><topic>Dibutyl phthalate</topic><topic>Fluorescence</topic><topic>Humic acids</topic><topic>Inhibitors</topic><topic>Relative humidity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Song, Hyerin</creatorcontrib><creatorcontrib>Lim, Hyun Jeong</creatorcontrib><creatorcontrib>Son, Ahjeong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>MEDLINE - Academic</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Song, Hyerin</au><au>Lim, Hyun Jeong</au><au>Son, Ahjeong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an aptasensor for dibutyl phthalate detection and the elucidation of assay inhibition factors</atitle><jtitle>RSC advances</jtitle><addtitle>RSC Adv</addtitle><date>2024-06-27</date><risdate>2024</risdate><volume>14</volume><issue>29</issue><spage>2585</spage><epage>2594</epage><pages>2585-2594</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>We developed a fluorescence aptasensor (hereafter 'SG-aptasensor') using SYBR Green I, a newly truncated 20-mer aptamer, and probe DNA to detect dibutyl phthalate (DBP). The detection range of DBP was 0.1-100 ng L
−1
with 0.08 ng L
−1
as the limit of detection. To adapt the assay to environmental samples in the near future, possible inhibition factors (experimental and environmental) have been tested and reported. The experimental inhibitors included the incubation time, temperature, pH, and ionic strength. Consequently, temperature (2-25 °C) and pH (7.0-9.0) ranges did not significantly inhibit the assay. The incubation time required for sufficient reaction was at least 4 h, and a relative humidity <20% may have induced fluorescence quenching. Tris-HCl-based incubation buffer with excess ionic strength (more than 0.2 M NaCl) demonstrated an abnormal increase in fluorescence. Environmental inhibitors including cations (Mg
2+
, Ca
2+
, and Cu
2+
) and humic acids were tested. The fluorescence signal was significantly reduced (∼99%) by 100 mM Cu
2+
compared to that by 0 mM Cu
2+
. In contrast, the reduction in fluorescence signal was marginal (<15%) when Mg
2+
or Ca
2+
ions were present. Inhibition of the assay was observed (∼28%) in the presence of 100 mg L
−1
humic acids.
DBP detection using SG-aptasensor and the elucidation of inhibition effects.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>38946763</pmid><doi>10.1039/d4ra03045a</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0001-6210-0769</orcidid><oa>free_for_read</oa></addata></record> |
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| language | eng |
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| source | DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Assaying Calcium ions Copper Dibutyl phthalate Fluorescence Humic acids Inhibitors Relative humidity |
| title | Development of an aptasensor for dibutyl phthalate detection and the elucidation of assay inhibition factors |
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