Redox-reversible siderophore-based catalyst anchoring within cross-linked artificial metalloenzyme aggregates enables enantioselectivity switching
The immobilisation of artificial metalloenzymes (ArMs) holds promise for the implementation of new biocatalytic reactions. We present the synthesis of cross-linked artificial metalloenzyme aggregates (CLArMAs) with excellent recyclability, as an alternative to carrier-based immobilisation strategies...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2024-05, Vol.6 (42), p.549-5493 |
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| creator | Miller, Alex H Thompson, Seán A Blagova, Elena V Wilson, Keith S Grogan, Gideon Duhme-Klair, Anne-K |
| description | The immobilisation of artificial metalloenzymes (ArMs) holds promise for the implementation of new biocatalytic reactions. We present the synthesis of cross-linked artificial metalloenzyme aggregates (CLArMAs) with excellent recyclability, as an alternative to carrier-based immobilisation strategies. Furthermore, iron-siderophore supramolecular anchoring facilitates redox-triggered cofactor release, enabling CLArMAs to be recharged with alternative cofactors for diverse selectivity.
The cross-linking of redox-reversible artificial metalloenzyme aggregates produces recyclable protein microparticles that enable catalyst replacement and hence catalyst-controlled enantioselectivity switching. |
| doi_str_mv | 10.1039/d4cc01158a |
| format | Article |
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| source | Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Aggregates Chemical synthesis Chemistry Crosslinking Enantiomers Immobilization Recyclability |
| title | Redox-reversible siderophore-based catalyst anchoring within cross-linked artificial metalloenzyme aggregates enables enantioselectivity switching |
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