Automatic detection of fluorescent droplets for droplet digital PCR: a device capable of processing multiple microscope images

Droplet digital PCR (ddPCR) is recognized as a high-precision method for nucleic acid quantification, extensively utilized in biomedical research and clinical diagnostics. This technique employs microfluidic technology to partition the nucleic acid-containing reaction mixture into discrete droplets...

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Veröffentlicht in:	Analyst (London) 2024-10, Vol.149 (21), p.5213-5224
Hauptverfasser:	Mao, Kaihao, Tao, Ye, Guo, Wenshang, Yang, Qisheng, Zhao, Meiying, Meng, Xiangyu, Zhang, Yinghao, Ren, Yukun
Format:	Artikel
Sprache:	eng
Schlagworte:	Accuracy Algorithms Digital imaging Droplets Fluorescence Image analysis Image enhancement Image processing Microscopy Nucleic acids Self-assembly Task complexity Technology assessment
Online-Zugang:	Volltext
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