Naringin ameliorates liver fibrosis in zebrafish by modulating IDO1-mediated lipid metabolism and inflammatory infiltration
Liver fibrosis (LF) is an important reparative process in response to acute or chronic hepatic injury, which has the potential to advance towards cirrhosis and hepatocellular carcinoma. Dietary naringin consumption contributes to protection against LF in animal studies, while the exact protective me...
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| Veröffentlicht in: | Food & function 2023-11, Vol.14 (23), p.1347-1361 |
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| creator | Qin, Meng-chen Li, Jun-jie Zheng, Yan-tao Li, Yun-jia Zhang, Yu-xue Ou, Rou-xuan He, Wei-yi Zhao, Jia-min Liu, Su-tong Liu, Ming-hao Lin, Hai-yan Gao, Lei |
| description | Liver fibrosis (LF) is an important reparative process in response to acute or chronic hepatic injury, which has the potential to advance towards cirrhosis and hepatocellular carcinoma. Dietary naringin consumption contributes to protection against LF in animal studies, while the exact protective mechanism of naringin remains unclear. This study aimed to investigate the molecular mechanisms behind the potential protective effect of naringin against TAA-induced LF in zebrafish. In this study, we utilized zebrafish to create the LF model and investigate the therapeutic mechanism of naringin. Firstly, we evaluated the changes in hepatic fibrosis and lipid accumulation in the liver following naringin treatment with oil red O, Nile red, and Sirius red and immunohistochemistry. In addition, we employed an ROS probe to directly measure oxidative stress and monitor inflammatory cell migration in a zebrafish transgenic line. Morpholino was used in the knockdown of IDO1 in order to verify its vital role in LF. Our findings demonstrated that naringin exhibited anti-inflammatory and anti-fibrotic action in conjunction with a reversal in lipid accumulation, oxidative stress and suppression of macrophage infiltration and activation of hepatic stellate cells. Furthermore, the results showed that the antifibrotic effect of naringin was removed upon IDO1 knockdown, proving that naringin exerts a protective effect by regulating IDO1. Naringin demonstrates remarkable protective effects against LF, effectively counteracting inflammation and hepatic steatosis in zebrafish liver. These findings suggest that naringin may function as an effective IDO1 inhibitor, holding the potential for clinical translation as a therapeutic agent for the treatment of LF.
Liver fibrosis (LF) is an important reparative process in response to acute or chronic hepatic injury, which has the potential to advance towards cirrhosis and hepatocellular carcinoma. |
| doi_str_mv | 10.1039/d3fo03858k |
| format | Article |
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Liver fibrosis (LF) is an important reparative process in response to acute or chronic hepatic injury, which has the potential to advance towards cirrhosis and hepatocellular carcinoma.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/d3fo03858k</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Accumulation ; Cell activation ; Cirrhosis ; Danio rerio ; Diet ; Fatty liver ; Fibrosis ; Hepatocellular carcinoma ; Immunohistochemistry ; Infiltration ; Inflammation ; Leukocyte migration ; Lipid metabolism ; Lipids ; Liver ; Macrophages ; Molecular modelling ; Oxidative stress ; Pharmacology ; Steatosis ; Stellate cells ; Zebrafish</subject><ispartof>Food & function, 2023-11, Vol.14 (23), p.1347-1361</ispartof><rights>Copyright Royal Society of Chemistry 2023</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c314t-683db722392c6e966db73d3f772733b207b90267bfb9da0772cedb95261b87873</citedby><cites>FETCH-LOGICAL-c314t-683db722392c6e966db73d3f772733b207b90267bfb9da0772cedb95261b87873</cites><orcidid>0000-0001-9030-390X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids></links><search><creatorcontrib>Qin, Meng-chen</creatorcontrib><creatorcontrib>Li, Jun-jie</creatorcontrib><creatorcontrib>Zheng, Yan-tao</creatorcontrib><creatorcontrib>Li, Yun-jia</creatorcontrib><creatorcontrib>Zhang, Yu-xue</creatorcontrib><creatorcontrib>Ou, Rou-xuan</creatorcontrib><creatorcontrib>He, Wei-yi</creatorcontrib><creatorcontrib>Zhao, Jia-min</creatorcontrib><creatorcontrib>Liu, Su-tong</creatorcontrib><creatorcontrib>Liu, Ming-hao</creatorcontrib><creatorcontrib>Lin, Hai-yan</creatorcontrib><creatorcontrib>Gao, Lei</creatorcontrib><title>Naringin ameliorates liver fibrosis in zebrafish by modulating IDO1-mediated lipid metabolism and inflammatory infiltration</title><title>Food & function</title><description>Liver fibrosis (LF) is an important reparative process in response to acute or chronic hepatic injury, which has the potential to advance towards cirrhosis and hepatocellular carcinoma. Dietary naringin consumption contributes to protection against LF in animal studies, while the exact protective mechanism of naringin remains unclear. This study aimed to investigate the molecular mechanisms behind the potential protective effect of naringin against TAA-induced LF in zebrafish. In this study, we utilized zebrafish to create the LF model and investigate the therapeutic mechanism of naringin. Firstly, we evaluated the changes in hepatic fibrosis and lipid accumulation in the liver following naringin treatment with oil red O, Nile red, and Sirius red and immunohistochemistry. In addition, we employed an ROS probe to directly measure oxidative stress and monitor inflammatory cell migration in a zebrafish transgenic line. Morpholino was used in the knockdown of IDO1 in order to verify its vital role in LF. Our findings demonstrated that naringin exhibited anti-inflammatory and anti-fibrotic action in conjunction with a reversal in lipid accumulation, oxidative stress and suppression of macrophage infiltration and activation of hepatic stellate cells. Furthermore, the results showed that the antifibrotic effect of naringin was removed upon IDO1 knockdown, proving that naringin exerts a protective effect by regulating IDO1. Naringin demonstrates remarkable protective effects against LF, effectively counteracting inflammation and hepatic steatosis in zebrafish liver. These findings suggest that naringin may function as an effective IDO1 inhibitor, holding the potential for clinical translation as a therapeutic agent for the treatment of LF.
Liver fibrosis (LF) is an important reparative process in response to acute or chronic hepatic injury, which has the potential to advance towards cirrhosis and hepatocellular carcinoma.</description><subject>Accumulation</subject><subject>Cell activation</subject><subject>Cirrhosis</subject><subject>Danio rerio</subject><subject>Diet</subject><subject>Fatty liver</subject><subject>Fibrosis</subject><subject>Hepatocellular carcinoma</subject><subject>Immunohistochemistry</subject><subject>Infiltration</subject><subject>Inflammation</subject><subject>Leukocyte migration</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Liver</subject><subject>Macrophages</subject><subject>Molecular modelling</subject><subject>Oxidative stress</subject><subject>Pharmacology</subject><subject>Steatosis</subject><subject>Stellate cells</subject><subject>Zebrafish</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><recordid>eNpdkctLAzEQxhdRsKgX70LAiwir2aTN4yj1icVeFLwtySarqcmmJluh-s87tT7AXJLJ_L5JZr6i2K_wSYWpPDW0jZiKkXjZKAYED0nJRvhx8-c8lGy72Mt5hmFRKYUUg-LjTiXXPbkOqWC9i0n1NiPv3mxCrdMpZpcRZN-tTqp1-RnpJQrRLLzqQYduzqdVGaxxoDOgmzuDgu2Vjt7lgFRnQN16FYLqY1quAud7eMXFbrfYapXPdu973ykeLi_ux9flZHp1Mz6blA2thn3JBDWaE0IlaZiVjEFEoVfOCadUE8y1xIRx3WppFIbrxhotR4RVWnDB6U5xtK47T_F1YXNfB5cb673qbFzkmgjBRlCXrNDDf-gsLlIHvwNKwmy5lEOgjtdUA_PJybb1PLmg0rKucL2yoj6nl9MvK24BPljDKTe_3J9V9BNuOIbM</recordid><startdate>20231127</startdate><enddate>20231127</enddate><creator>Qin, Meng-chen</creator><creator>Li, Jun-jie</creator><creator>Zheng, Yan-tao</creator><creator>Li, Yun-jia</creator><creator>Zhang, Yu-xue</creator><creator>Ou, Rou-xuan</creator><creator>He, Wei-yi</creator><creator>Zhao, Jia-min</creator><creator>Liu, Su-tong</creator><creator>Liu, Ming-hao</creator><creator>Lin, Hai-yan</creator><creator>Gao, Lei</creator><general>Royal Society of Chemistry</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9030-390X</orcidid></search><sort><creationdate>20231127</creationdate><title>Naringin ameliorates liver fibrosis in zebrafish by modulating IDO1-mediated lipid metabolism and inflammatory infiltration</title><author>Qin, Meng-chen ; Li, Jun-jie ; Zheng, Yan-tao ; Li, Yun-jia ; Zhang, Yu-xue ; Ou, Rou-xuan ; He, Wei-yi ; Zhao, Jia-min ; Liu, Su-tong ; Liu, Ming-hao ; Lin, Hai-yan ; Gao, Lei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c314t-683db722392c6e966db73d3f772733b207b90267bfb9da0772cedb95261b87873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Accumulation</topic><topic>Cell activation</topic><topic>Cirrhosis</topic><topic>Danio rerio</topic><topic>Diet</topic><topic>Fatty liver</topic><topic>Fibrosis</topic><topic>Hepatocellular carcinoma</topic><topic>Immunohistochemistry</topic><topic>Infiltration</topic><topic>Inflammation</topic><topic>Leukocyte migration</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Liver</topic><topic>Macrophages</topic><topic>Molecular modelling</topic><topic>Oxidative stress</topic><topic>Pharmacology</topic><topic>Steatosis</topic><topic>Stellate cells</topic><topic>Zebrafish</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qin, Meng-chen</creatorcontrib><creatorcontrib>Li, Jun-jie</creatorcontrib><creatorcontrib>Zheng, Yan-tao</creatorcontrib><creatorcontrib>Li, Yun-jia</creatorcontrib><creatorcontrib>Zhang, Yu-xue</creatorcontrib><creatorcontrib>Ou, Rou-xuan</creatorcontrib><creatorcontrib>He, Wei-yi</creatorcontrib><creatorcontrib>Zhao, Jia-min</creatorcontrib><creatorcontrib>Liu, Su-tong</creatorcontrib><creatorcontrib>Liu, Ming-hao</creatorcontrib><creatorcontrib>Lin, Hai-yan</creatorcontrib><creatorcontrib>Gao, Lei</creatorcontrib><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qin, Meng-chen</au><au>Li, Jun-jie</au><au>Zheng, Yan-tao</au><au>Li, Yun-jia</au><au>Zhang, Yu-xue</au><au>Ou, Rou-xuan</au><au>He, Wei-yi</au><au>Zhao, Jia-min</au><au>Liu, Su-tong</au><au>Liu, Ming-hao</au><au>Lin, Hai-yan</au><au>Gao, Lei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Naringin ameliorates liver fibrosis in zebrafish by modulating IDO1-mediated lipid metabolism and inflammatory infiltration</atitle><jtitle>Food & function</jtitle><date>2023-11-27</date><risdate>2023</risdate><volume>14</volume><issue>23</issue><spage>1347</spage><epage>1361</epage><pages>1347-1361</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>Liver fibrosis (LF) is an important reparative process in response to acute or chronic hepatic injury, which has the potential to advance towards cirrhosis and hepatocellular carcinoma. Dietary naringin consumption contributes to protection against LF in animal studies, while the exact protective mechanism of naringin remains unclear. This study aimed to investigate the molecular mechanisms behind the potential protective effect of naringin against TAA-induced LF in zebrafish. In this study, we utilized zebrafish to create the LF model and investigate the therapeutic mechanism of naringin. Firstly, we evaluated the changes in hepatic fibrosis and lipid accumulation in the liver following naringin treatment with oil red O, Nile red, and Sirius red and immunohistochemistry. In addition, we employed an ROS probe to directly measure oxidative stress and monitor inflammatory cell migration in a zebrafish transgenic line. Morpholino was used in the knockdown of IDO1 in order to verify its vital role in LF. Our findings demonstrated that naringin exhibited anti-inflammatory and anti-fibrotic action in conjunction with a reversal in lipid accumulation, oxidative stress and suppression of macrophage infiltration and activation of hepatic stellate cells. Furthermore, the results showed that the antifibrotic effect of naringin was removed upon IDO1 knockdown, proving that naringin exerts a protective effect by regulating IDO1. Naringin demonstrates remarkable protective effects against LF, effectively counteracting inflammation and hepatic steatosis in zebrafish liver. These findings suggest that naringin may function as an effective IDO1 inhibitor, holding the potential for clinical translation as a therapeutic agent for the treatment of LF.
Liver fibrosis (LF) is an important reparative process in response to acute or chronic hepatic injury, which has the potential to advance towards cirrhosis and hepatocellular carcinoma.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/d3fo03858k</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-9030-390X</orcidid></addata></record> |
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| ispartof | Food & function, 2023-11, Vol.14 (23), p.1347-1361 |
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| source | Royal Society Of Chemistry Journals |
| subjects | Accumulation Cell activation Cirrhosis Danio rerio Diet Fatty liver Fibrosis Hepatocellular carcinoma Immunohistochemistry Infiltration Inflammation Leukocyte migration Lipid metabolism Lipids Liver Macrophages Molecular modelling Oxidative stress Pharmacology Steatosis Stellate cells Zebrafish |
| title | Naringin ameliorates liver fibrosis in zebrafish by modulating IDO1-mediated lipid metabolism and inflammatory infiltration |
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