Double blocking gap-filling-ligation coupled with cascade isothermal amplification for ultrasensitive quantification of N 6 -methyladenosine

We developed a method for quantifying N 6 -methyladenosine at one-nucleotide resolution based on double blocking gap-filling-ligation and cascade isothermal amplification. This proposed method can detect as low as 1 fM target RNA, achieving selectivity up to approximately 100-fold between m 6 A and...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2023-09, Vol.59 (72), p.10769-10772
Hauptverfasser:	Liu, Weiliang, Su, Fengxia, Gao, Kejian, Chen, Desheng, Li, Zhengping
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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