SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids
Patient derived organoids have the potential to improve the physiological relevance of in vitro disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2023-03, Vol.59 (22), p.3249-3252 |
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| creator | Skinner, William H Robinson, Nicola Hardisty, Gareth R Fleming, Holly Geddis, Ailsa Bradley, Mark Gray, Robert D Campbell, Colin J |
| description | Patient derived organoids have the potential to improve the physiological relevance of
in vitro
disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.
SERS microsensors (SERS-MS) are polymer microparticles coated in gold nanoparticles and functionalised with mercaptobenzoic acid. SERS-MS were incorporated into human airway organoids to measured pH optically. |
| doi_str_mv | 10.1039/d2cc06582g |
| format | Article |
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in vitro
disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.
SERS microsensors (SERS-MS) are polymer microparticles coated in gold nanoparticles and functionalised with mercaptobenzoic acid. SERS-MS were incorporated into human airway organoids to measured pH optically.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/d2cc06582g</identifier><identifier>PMID: 36815668</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Cellular structure ; Extracellular Matrix ; Gold - chemistry ; Humans ; Hydrogen-Ion Concentration ; Metal Nanoparticles - chemistry ; Nanoparticles ; Organoids - metabolism ; Raman spectra ; Self-assembly ; Spectrum Analysis, Raman ; Stem Cells</subject><ispartof>Chemical communications (Cambridge, England), 2023-03, Vol.59 (22), p.3249-3252</ispartof><rights>Copyright Royal Society of Chemistry 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c373t-68e2d9f7dac26192b9f6f4cdcf0e74a8377ce6b55353524127032c8e315c5ca53</citedby><cites>FETCH-LOGICAL-c373t-68e2d9f7dac26192b9f6f4cdcf0e74a8377ce6b55353524127032c8e315c5ca53</cites><orcidid>0000-0003-1917-6105 ; 0000-0002-4307-8356 ; 0000-0001-7289-4566 ; 0000-0001-7893-1575</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36815668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Skinner, William H</creatorcontrib><creatorcontrib>Robinson, Nicola</creatorcontrib><creatorcontrib>Hardisty, Gareth R</creatorcontrib><creatorcontrib>Fleming, Holly</creatorcontrib><creatorcontrib>Geddis, Ailsa</creatorcontrib><creatorcontrib>Bradley, Mark</creatorcontrib><creatorcontrib>Gray, Robert D</creatorcontrib><creatorcontrib>Campbell, Colin J</creatorcontrib><title>SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>Patient derived organoids have the potential to improve the physiological relevance of
in vitro
disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.
SERS microsensors (SERS-MS) are polymer microparticles coated in gold nanoparticles and functionalised with mercaptobenzoic acid. SERS-MS were incorporated into human airway organoids to measured pH optically.</description><subject>Cellular structure</subject><subject>Extracellular Matrix</subject><subject>Gold - chemistry</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Nanoparticles</subject><subject>Organoids - metabolism</subject><subject>Raman spectra</subject><subject>Self-assembly</subject><subject>Spectrum Analysis, Raman</subject><subject>Stem Cells</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0VtLBCEUB3CJosvWS--F0EsEU6OOl3mMaduCIugCvQ2uHmtiZ9x0pujb57ZdIH1Q8efh8BehXZIfk5yVJ5Yakwuu6NMK2iRMFBkv1OPqYs_LTLKCb6CtGF_yNAhX62iDCUW4EGoTubvx7R1uGxN8hC76ELHzAc8vcAs6DgFa6PqImw73z4BnQzpi3Vk8rq6xdzj20GIDsxm2EJo3sPh5aHUiTXjXH9iHJ935xsZttOb0LMLO9zpCD-fj--oiu7qZXFanV5lhkvWZUEBt6aTVhgpS0mnphCuMNS4HWWjFpDQgppyzNGlBqMwZNQoY4YYbzdkIHS7rzoN_HSD2ddvERX-6Az_EmkpZppdK0EQP_tEXP4QudZeUEpyxRJM6WqpFQDGAq-ehaXX4qEleL9Kvz2hVfaU_SXj_u-QwbcH-0p-4E9hbghDN7-3f97FPC8CIcA</recordid><startdate>20230314</startdate><enddate>20230314</enddate><creator>Skinner, William H</creator><creator>Robinson, Nicola</creator><creator>Hardisty, Gareth R</creator><creator>Fleming, Holly</creator><creator>Geddis, Ailsa</creator><creator>Bradley, Mark</creator><creator>Gray, Robert D</creator><creator>Campbell, Colin J</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1917-6105</orcidid><orcidid>https://orcid.org/0000-0002-4307-8356</orcidid><orcidid>https://orcid.org/0000-0001-7289-4566</orcidid><orcidid>https://orcid.org/0000-0001-7893-1575</orcidid></search><sort><creationdate>20230314</creationdate><title>SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids</title><author>Skinner, William H ; 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in vitro
disease models. However, the 3D architecture of these self-assembled cellular structures makes probing their biochemistry more complex than in traditional 2D culture. We explore the application of surface enhanced Raman scattering microsensors (SERS-MS) to probe local pH gradients within patient derived airway organoid cultures. SERS-MS consist of solid polymer cores decorated with surface immobilised gold nanoparticles which are functionalised with pH sensitive reporter molecule 4-mercaptobenzoic acid (MBA). We demonstrate that by mixing SERS-MS into the extracellular matrix (ECM) of airway organoid cultures the probes can be engulfed by expanding organoids and report on local pH in the organoid lumen and ECM.
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| ispartof | Chemical communications (Cambridge, England), 2023-03, Vol.59 (22), p.3249-3252 |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Cellular structure Extracellular Matrix Gold - chemistry Humans Hydrogen-Ion Concentration Metal Nanoparticles - chemistry Nanoparticles Organoids - metabolism Raman spectra Self-assembly Spectrum Analysis, Raman Stem Cells |
| title | SERS microsensors for pH measurements in the lumen and ECM of stem cell derived human airway organoids |
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