Characterization of the T4 gp32-ssDNA complex by native, cross-linking, and ultraviolet photodissociation mass spectrometry

Protein-DNA interactions play crucial roles in DNA replication across all living organisms. Here, we apply a suite of mass spectrometry (MS) tools to characterize a protein-ssDNA complex, T4 gp32·ssDNA, with results that both support previous studies and simultaneously uncover novel insight into thi...

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Veröffentlicht in:	Chemical science (Cambridge) 2021-10, Vol.12 (41), p.13764-13776
Hauptverfasser:	Blevins, Molly S, Walker, Jada N, Schaub, Jeffrey M, Finkelstein, Ilya J, Brodbelt, Jennifer S
Format:	Artikel
Sprache:	eng
Schlagworte:	Binding sites Chemistry Coordination compounds Crosslinking Crystallography Interrogation Mass spectrometry Monomers Photodissociation Proteins Scientific imaging Spectroscopy Stoichiometry Zinc
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