Blue-conversion of organic dyes produces artifacts in multicolor fluorescence imaging

Multicolor fluorescence imaging is a powerful tool visualizing the spatiotemporal relationship among biomolecules. Here, we report that commonly employed organic dyes exhibit a blue-conversion phenomenon, which can produce severe multicolor image artifacts leading to false-positive colocalization by...

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Veröffentlicht in:	Chemical science (Cambridge) 2021-06, Vol.12 (25), p.866-8667
Hauptverfasser:	Kim, Do-Hyeon, Chang, Yeonho, Park, Soyeon, Jeong, Min Gyu, Kwon, Yonghoon, Zhou, Kai, Noh, Jungeun, Choi, Yun-Kyu, Hong, Triet Minh, Chang, Young-Tae, Ryu, Sung Ho
Format:	Artikel
Sprache:	eng
Schlagworte:	Biomolecules Chemistry Conversion Dyes Fluorescence Imaging
Online-Zugang:	Volltext
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