Dual-enzyme and NADPH co-embedded organic-inorganic hybrid nanoflowers prepared using biomimetic mineralization for the asymmetric synthesis of ()-(−)-pantolactone

An effective biocatalytic cascade was developed by encapsulating a conjugated polyketone reductase (CPR), glucose dehydrogenase (GDH) and the coenzyme NADP + in organic-inorganic hybrid nanoflowers (hNFs) for the asymmetric reduction of ketopantolactone (KPL) to synthesize ( R )-(−)-pantolactone [( R )-PL]. When Cdu CPR and Tg GDH were confined in the SA-coated hNFs [ Cdu CPR/ Tg GDH@Ca 3 (PO 4 ) 2 ], both of them had excellent reusability and high stability. Notably, the SA-coated hNF reactor successfully catalyzed the asymmetric synthesis of ( R )-PL, which exhibited satisfactory stereoselectivity and promoted reusability in repeated batches. The SA coating functioned to competently immobilize the coenzyme NADP + on the Cdu CPR/ Tg GDH@Ca 3 (PO 4 ) 2 hNFs, which maintained the maximum bioactivity to synthesize ( R )-PL without exogenous coenzyme addition. Consequently, the facile immobilization method should be useful as a common approach for multi-enzyme cascade reactions and has potential applications in the biocatalytic industry. A SA-coated hNF composite was designed to co-immobilize dual enzymes and coenzyme NADP + for the asymmetric synthesis of d -(−)-pantolactone.