Detecting nitrile-containing small molecules by infrared photothermal microscopy
The use of infrared (IR) photothermal microscopy (IR-PTM) is emerging for imaging chemical substances in various samples. In this research, we demonstrated the use of a nitrile group as a vibrational tag to image target molecules in the low water-background region. We performed IR photothermal imagi...
Gespeichert in:
| Veröffentlicht in: | Analyst (London) 2021-04, Vol.146 (7), p.237-2312 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 2312 |
|---|---|
| container_issue | 7 |
| container_start_page | 237 |
| container_title | Analyst (London) |
| container_volume | 146 |
| creator | Tai, Fangfang Koike, Kota Kawagoe, Hiroyuki Ando, Jun Kumamoto, Yasuaki Smith, Nicholas I Sodeoka, Mikiko Fujita, Katsumasa |
| description | The use of infrared (IR) photothermal microscopy (IR-PTM) is emerging for imaging chemical substances in various samples. In this research, we demonstrated the use of a nitrile group as a vibrational tag to image target molecules in the low water-background region. We performed IR photothermal imaging of trifluoromethoxy carbonyl cyanide phenylhydrazone (FCCP) in cells and confirmed the high spatial resolution by photothermal detection using visible light as a probe beam. We imaged FCCP-treated HeLa cells and confirmed that the photothermal signal was indeed produced from the vibrational tag in lipid droplets. We also compared the results with nitrile imaging by stimulated Raman scattering (SRS) microscopy. From both the calculated and experimental results, IR-PTM demonstrated a signal-to-noise ratio (SNR) several tens of times better than that of SRS microscopy on the basis of the same power input.
We demonstrated IR photothermal imaging of trifluoromethoxy carbonyl cyanide phenylhydrazone (FCCP) in cells. The result indicates that a nitrile group can be used as a vibrational tag to image target molecules. |
| doi_str_mv | 10.1039/d0an02198a |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1039_D0AN02198A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2492661531</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-1411543e97c6dd3a2022742d756287993b86031ec9c00f218b43ac0bf4853e223</originalsourceid><addsrcrecordid>eNpd0c9LwzAUB_AgipvTi3el4EWE6suvNjmOzV8w1IOeS5qmrqNtapIe9t_buTnBU3jJh8d73yB0juEWA5V3BagWCJZCHaAxpgmLOSfiEI0BgMYk4WyETrxfDSUGDsdoRGlCABgbo7e5CUaHqv2M2iq4qjaxtm1QVbu58o2q66ixtdF9bXyUr6OqLZ1ypoi6pQ02LI0bTNRU2lmvbbc-RUelqr05250T9PFw_z57ihevj8-z6SLWDGiIMcOYM2pkqpOioIoAISkjRcoTIlIpaS4SoNhoqQFKgkXOqNKQl0xwagihE3S97ds5-9UbH7Km8trUtWqN7X1GmCRJgjnFA736R1e2d-0wXUY4CMGJlGJQN1u12cQ7U2adqxrl1hmGbJNzNofpy0_O0wFf7lr2eWOKPf0NdgAXW-C83r_-fRT9BoNGgJ0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2508852998</pqid></control><display><type>article</type><title>Detecting nitrile-containing small molecules by infrared photothermal microscopy</title><source>MEDLINE</source><source>Royal Society of Chemistry Journals Archive (1841-2007)</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Tai, Fangfang ; Koike, Kota ; Kawagoe, Hiroyuki ; Ando, Jun ; Kumamoto, Yasuaki ; Smith, Nicholas I ; Sodeoka, Mikiko ; Fujita, Katsumasa</creator><creatorcontrib>Tai, Fangfang ; Koike, Kota ; Kawagoe, Hiroyuki ; Ando, Jun ; Kumamoto, Yasuaki ; Smith, Nicholas I ; Sodeoka, Mikiko ; Fujita, Katsumasa</creatorcontrib><description>The use of infrared (IR) photothermal microscopy (IR-PTM) is emerging for imaging chemical substances in various samples. In this research, we demonstrated the use of a nitrile group as a vibrational tag to image target molecules in the low water-background region. We performed IR photothermal imaging of trifluoromethoxy carbonyl cyanide phenylhydrazone (FCCP) in cells and confirmed the high spatial resolution by photothermal detection using visible light as a probe beam. We imaged FCCP-treated HeLa cells and confirmed that the photothermal signal was indeed produced from the vibrational tag in lipid droplets. We also compared the results with nitrile imaging by stimulated Raman scattering (SRS) microscopy. From both the calculated and experimental results, IR-PTM demonstrated a signal-to-noise ratio (SNR) several tens of times better than that of SRS microscopy on the basis of the same power input.
We demonstrated IR photothermal imaging of trifluoromethoxy carbonyl cyanide phenylhydrazone (FCCP) in cells. The result indicates that a nitrile group can be used as a vibrational tag to image target molecules.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/d0an02198a</identifier><identifier>PMID: 33620044</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Carbonyls ; HeLa Cells ; Humans ; Imaging ; Lipids ; Microscopy ; Nitriles ; Raman spectra ; Signal to noise ratio ; Spatial resolution ; Spectrum Analysis, Raman ; Target recognition ; Vibration</subject><ispartof>Analyst (London), 2021-04, Vol.146 (7), p.237-2312</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-1411543e97c6dd3a2022742d756287993b86031ec9c00f218b43ac0bf4853e223</citedby><cites>FETCH-LOGICAL-c403t-1411543e97c6dd3a2022742d756287993b86031ec9c00f218b43ac0bf4853e223</cites><orcidid>0000-0002-4398-288X ; 0000-0002-2284-375X ; 0000-0002-5631-5138 ; 0000-0002-1344-364X ; 0000-0002-0707-0808</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2831,2832,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33620044$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tai, Fangfang</creatorcontrib><creatorcontrib>Koike, Kota</creatorcontrib><creatorcontrib>Kawagoe, Hiroyuki</creatorcontrib><creatorcontrib>Ando, Jun</creatorcontrib><creatorcontrib>Kumamoto, Yasuaki</creatorcontrib><creatorcontrib>Smith, Nicholas I</creatorcontrib><creatorcontrib>Sodeoka, Mikiko</creatorcontrib><creatorcontrib>Fujita, Katsumasa</creatorcontrib><title>Detecting nitrile-containing small molecules by infrared photothermal microscopy</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>The use of infrared (IR) photothermal microscopy (IR-PTM) is emerging for imaging chemical substances in various samples. In this research, we demonstrated the use of a nitrile group as a vibrational tag to image target molecules in the low water-background region. We performed IR photothermal imaging of trifluoromethoxy carbonyl cyanide phenylhydrazone (FCCP) in cells and confirmed the high spatial resolution by photothermal detection using visible light as a probe beam. We imaged FCCP-treated HeLa cells and confirmed that the photothermal signal was indeed produced from the vibrational tag in lipid droplets. We also compared the results with nitrile imaging by stimulated Raman scattering (SRS) microscopy. From both the calculated and experimental results, IR-PTM demonstrated a signal-to-noise ratio (SNR) several tens of times better than that of SRS microscopy on the basis of the same power input.
We demonstrated IR photothermal imaging of trifluoromethoxy carbonyl cyanide phenylhydrazone (FCCP) in cells. The result indicates that a nitrile group can be used as a vibrational tag to image target molecules.</description><subject>Carbonyls</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Imaging</subject><subject>Lipids</subject><subject>Microscopy</subject><subject>Nitriles</subject><subject>Raman spectra</subject><subject>Signal to noise ratio</subject><subject>Spatial resolution</subject><subject>Spectrum Analysis, Raman</subject><subject>Target recognition</subject><subject>Vibration</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0c9LwzAUB_AgipvTi3el4EWE6suvNjmOzV8w1IOeS5qmrqNtapIe9t_buTnBU3jJh8d73yB0juEWA5V3BagWCJZCHaAxpgmLOSfiEI0BgMYk4WyETrxfDSUGDsdoRGlCABgbo7e5CUaHqv2M2iq4qjaxtm1QVbu58o2q66ixtdF9bXyUr6OqLZ1ypoi6pQ02LI0bTNRU2lmvbbc-RUelqr05250T9PFw_z57ihevj8-z6SLWDGiIMcOYM2pkqpOioIoAISkjRcoTIlIpaS4SoNhoqQFKgkXOqNKQl0xwagihE3S97ds5-9UbH7Km8trUtWqN7X1GmCRJgjnFA736R1e2d-0wXUY4CMGJlGJQN1u12cQ7U2adqxrl1hmGbJNzNofpy0_O0wFf7lr2eWOKPf0NdgAXW-C83r_-fRT9BoNGgJ0</recordid><startdate>20210407</startdate><enddate>20210407</enddate><creator>Tai, Fangfang</creator><creator>Koike, Kota</creator><creator>Kawagoe, Hiroyuki</creator><creator>Ando, Jun</creator><creator>Kumamoto, Yasuaki</creator><creator>Smith, Nicholas I</creator><creator>Sodeoka, Mikiko</creator><creator>Fujita, Katsumasa</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4398-288X</orcidid><orcidid>https://orcid.org/0000-0002-2284-375X</orcidid><orcidid>https://orcid.org/0000-0002-5631-5138</orcidid><orcidid>https://orcid.org/0000-0002-1344-364X</orcidid><orcidid>https://orcid.org/0000-0002-0707-0808</orcidid></search><sort><creationdate>20210407</creationdate><title>Detecting nitrile-containing small molecules by infrared photothermal microscopy</title><author>Tai, Fangfang ; Koike, Kota ; Kawagoe, Hiroyuki ; Ando, Jun ; Kumamoto, Yasuaki ; Smith, Nicholas I ; Sodeoka, Mikiko ; Fujita, Katsumasa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-1411543e97c6dd3a2022742d756287993b86031ec9c00f218b43ac0bf4853e223</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Carbonyls</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Imaging</topic><topic>Lipids</topic><topic>Microscopy</topic><topic>Nitriles</topic><topic>Raman spectra</topic><topic>Signal to noise ratio</topic><topic>Spatial resolution</topic><topic>Spectrum Analysis, Raman</topic><topic>Target recognition</topic><topic>Vibration</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tai, Fangfang</creatorcontrib><creatorcontrib>Koike, Kota</creatorcontrib><creatorcontrib>Kawagoe, Hiroyuki</creatorcontrib><creatorcontrib>Ando, Jun</creatorcontrib><creatorcontrib>Kumamoto, Yasuaki</creatorcontrib><creatorcontrib>Smith, Nicholas I</creatorcontrib><creatorcontrib>Sodeoka, Mikiko</creatorcontrib><creatorcontrib>Fujita, Katsumasa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tai, Fangfang</au><au>Koike, Kota</au><au>Kawagoe, Hiroyuki</au><au>Ando, Jun</au><au>Kumamoto, Yasuaki</au><au>Smith, Nicholas I</au><au>Sodeoka, Mikiko</au><au>Fujita, Katsumasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detecting nitrile-containing small molecules by infrared photothermal microscopy</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2021-04-07</date><risdate>2021</risdate><volume>146</volume><issue>7</issue><spage>237</spage><epage>2312</epage><pages>237-2312</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>The use of infrared (IR) photothermal microscopy (IR-PTM) is emerging for imaging chemical substances in various samples. In this research, we demonstrated the use of a nitrile group as a vibrational tag to image target molecules in the low water-background region. We performed IR photothermal imaging of trifluoromethoxy carbonyl cyanide phenylhydrazone (FCCP) in cells and confirmed the high spatial resolution by photothermal detection using visible light as a probe beam. We imaged FCCP-treated HeLa cells and confirmed that the photothermal signal was indeed produced from the vibrational tag in lipid droplets. We also compared the results with nitrile imaging by stimulated Raman scattering (SRS) microscopy. From both the calculated and experimental results, IR-PTM demonstrated a signal-to-noise ratio (SNR) several tens of times better than that of SRS microscopy on the basis of the same power input.
We demonstrated IR photothermal imaging of trifluoromethoxy carbonyl cyanide phenylhydrazone (FCCP) in cells. The result indicates that a nitrile group can be used as a vibrational tag to image target molecules.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>33620044</pmid><doi>10.1039/d0an02198a</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-4398-288X</orcidid><orcidid>https://orcid.org/0000-0002-2284-375X</orcidid><orcidid>https://orcid.org/0000-0002-5631-5138</orcidid><orcidid>https://orcid.org/0000-0002-1344-364X</orcidid><orcidid>https://orcid.org/0000-0002-0707-0808</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2654 |
| ispartof | Analyst (London), 2021-04, Vol.146 (7), p.237-2312 |
| issn | 0003-2654 1364-5528 |
| language | eng |
| recordid | cdi_crossref_primary_10_1039_D0AN02198A |
| source | MEDLINE; Royal Society of Chemistry Journals Archive (1841-2007); Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Carbonyls HeLa Cells Humans Imaging Lipids Microscopy Nitriles Raman spectra Signal to noise ratio Spatial resolution Spectrum Analysis, Raman Target recognition Vibration |
| title | Detecting nitrile-containing small molecules by infrared photothermal microscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T19%3A42%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detecting%20nitrile-containing%20small%20molecules%20by%20infrared%20photothermal%20microscopy&rft.jtitle=Analyst%20(London)&rft.au=Tai,%20Fangfang&rft.date=2021-04-07&rft.volume=146&rft.issue=7&rft.spage=237&rft.epage=2312&rft.pages=237-2312&rft.issn=0003-2654&rft.eissn=1364-5528&rft_id=info:doi/10.1039/d0an02198a&rft_dat=%3Cproquest_cross%3E2492661531%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2508852998&rft_id=info:pmid/33620044&rfr_iscdi=true |