An ionophore-based persistent luminescent Glow Sensor for sodium detection

Optical sensors have numerous positive attributes such as low invasiveness, miniaturizability, biocompatibility, and ease of signal transduction. Recently, there has been a strong research focus on using phosphorescent readout mechanisms, specifically from long-lifetime phosphorescent or persistent luminescence particles, for in vitro and in vivo sensors. Persistent luminescence readouts can avoid cellular autofluorescence during biological monitoring, leading to an improved signal-to-noise ratio over a more traditional fluorescence readout. In this study, we show for the first time an ionophore-based optical bulk optode sensor that utilizes persistent luminescence microparticles for ion detection. To achieve this, we combined long-lifetime strontium aluminate-based glow-in-the-dark microparticles with a non-fluorescent pH-responsive dye in a hydrophobic plasticized polymer membrane along with traditional ionophore-based optical sensor components to create a phosphorescent Glow Sensor. The non-fluorescent pH indicator dye gates the strontium aluminate luminescence signal so that it decreases in magnitude with increasing sodium concentration. We characterized the Glow Sensor in terms of emission lifetime, dynamic range, response time, reversibility, selectivity, and stability. A sodium-selective bulk-optode sensor is created by coupling persistent luminescence microparticles with a pH-sensitive dye through an ionophore-based detection mechanism.