Microfluidic epigenomic mapping technologies for precision medicine
Epigenomic mapping of tissue samples generates critical insights into genome-wide regulations of gene activities and expressions during normal development and disease processes. Epigenomic profiling using a low number of cells produced by patient and mouse samples presents new challenges to biotechn...
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| Veröffentlicht in: | Lab on a chip 2019-08, Vol.19 (16), p.263-265 |
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| creator | Deng, Chengyu Naler, Lynette B Lu, Chang |
| description | Epigenomic mapping of tissue samples generates critical insights into genome-wide regulations of gene activities and expressions during normal development and disease processes. Epigenomic profiling using a low number of cells produced by patient and mouse samples presents new challenges to biotechnologists. In this review, we first discuss the rationale and premise behind profiling epigenomes for precision medicine. We then examine the existing literature on applying microfluidics to facilitate low-input and high-throughput epigenomic profiling, with emphasis on technologies enabling interfacing with next-generation sequencing. We detail assays on studies of histone modifications, DNA methylation, 3D chromatin structures and non-coding RNAs. Finally, we discuss what the future may hold in terms of method development and translational potential.
A review of microfluidic technologies for epigenetic and epigenomic analyses. |
| doi_str_mv | 10.1039/c9lc00407f |
| format | Article |
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A review of microfluidic technologies for epigenetic and epigenomic analyses.</description><subject>Animals</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA methylation</subject><subject>Epigenomics - instrumentation</subject><subject>Gene sequencing</subject><subject>Humans</subject><subject>Mapping</subject><subject>Medicine</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Microfluidics</subject><subject>Precision Medicine</subject><issn>1473-0197</issn><issn>1473-0189</issn><issn>1473-0189</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkctLxDAQxoMovi_elYIXEVbzaNPmIkjxBSte9BzSdLJG2qQmW8H_3qyr6-M0A_Obbx4fQgcEnxHMxLkWncY4x6VZQ9skL9kEk0qsr3JRbqGdGF8wJkXOq020xQhjVYHpNqrvrQ7edKNtrc5gsDNwvk9pr4bBulk2B_3sfOdnFmJmfMiGANpG613WQ-qxDvbQhlFdhP2vuIuerq8e69vJ9OHmrr6cTnSB-XzCWEG0ESw3JRBDeVNpYKAwIXnTspJCKUpgOWlFoVvglGqmTMuFoFy1DW_YLrpY6g5jk2ZrcPOgOjkE26vwLr2y8m_F2Wc582-Sc1ESnCeBky-B4F9HiHPZ26ih65QDP0ZJKWeMkgrzhB7_Q1_8GFw6b0FVhciFWAieLqn0wxgDmNUyBMuFN7IW0_rTm-sEH_1ef4V-m5GAwyUQol5Vf8xlH4uBlKM</recordid><startdate>20190821</startdate><enddate>20190821</enddate><creator>Deng, Chengyu</creator><creator>Naler, Lynette B</creator><creator>Lu, Chang</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7TB</scope><scope>7U5</scope><scope>8FD</scope><scope>FR3</scope><scope>L7M</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0003-0181-5888</orcidid><orcidid>https://orcid.org/0000-0002-1336-3236</orcidid></search><sort><creationdate>20190821</creationdate><title>Microfluidic epigenomic mapping technologies for precision medicine</title><author>Deng, Chengyu ; Naler, Lynette B ; Lu, Chang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c506t-3351cf934f7e1f26b8ce3ea0114bd372e797e341d95cde622c3afd69926adb6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA methylation</topic><topic>Epigenomics - instrumentation</topic><topic>Gene sequencing</topic><topic>Humans</topic><topic>Mapping</topic><topic>Medicine</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Microfluidics</topic><topic>Precision Medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Deng, Chengyu</creatorcontrib><creatorcontrib>Naler, Lynette B</creatorcontrib><creatorcontrib>Lu, Chang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Lab on a chip</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Deng, Chengyu</au><au>Naler, Lynette B</au><au>Lu, Chang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microfluidic epigenomic mapping technologies for precision medicine</atitle><jtitle>Lab on a chip</jtitle><addtitle>Lab Chip</addtitle><date>2019-08-21</date><risdate>2019</risdate><volume>19</volume><issue>16</issue><spage>263</spage><epage>265</epage><pages>263-265</pages><issn>1473-0197</issn><issn>1473-0189</issn><eissn>1473-0189</eissn><abstract>Epigenomic mapping of tissue samples generates critical insights into genome-wide regulations of gene activities and expressions during normal development and disease processes. 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| fulltext | fulltext |
| identifier | ISSN: 1473-0197 |
| ispartof | Lab on a chip, 2019-08, Vol.19 (16), p.263-265 |
| issn | 1473-0197 1473-0189 1473-0189 |
| language | eng |
| recordid | cdi_crossref_primary_10_1039_C9LC00407F |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Animals Deoxyribonucleic acid DNA DNA methylation Epigenomics - instrumentation Gene sequencing Humans Mapping Medicine Microfluidic Analytical Techniques - instrumentation Microfluidics Precision Medicine |
| title | Microfluidic epigenomic mapping technologies for precision medicine |
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