Formononetin inhibits human bladder cancer cell proliferation and invasiveness via regulation of miR-21 and PTEN
The isoflavone formononetin is the main active component of Astragalus membranaceus and possesses anti-tumorigenic properties. However, the role of formononetin in human bladder cancer (BCa) has not been fully elucidated. The aim of the present study was to investigate the anti-tumor effects of form...
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| Veröffentlicht in: | Food & function 2017-03, Vol.8 (3), p.161-166 |
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| creator | Wu, Yiying Zhang, Xing Li, Zhengzhao Yan, Haibiao Qin, Jian Li, Tianyu |
| description | The isoflavone formononetin is the main active component of
Astragalus membranaceus
and possesses anti-tumorigenic properties. However, the role of formononetin in human bladder cancer (BCa) has not been fully elucidated. The aim of the present study was to investigate the anti-tumor effects of formononetin on BCa cells and its potential molecular mechanism. T24 cells were treated with different concentrations of formononetin, and then the cell proliferation was assessed by MTT assay, cell apoptosis by Hoechst 33258 stain assay, cell invasiveness by transwell invasion assay, microRNA-21 (miR-21) expression by real-time PCR and the protein level of phosphatase and tensin homolog (PTEN) and phosphorylated homolog of Akt (p-Akt) by western blotting. The results showed that formononetin significantly inhibited the proliferation of T24 cells in a time- and dose-dependent manner. T24 cells treated with formononetin displayed obvious morphological changes of apoptosis and lower invasiveness. In addition, miR-21 expression was significantly decreased in formononetin-treated T24 cells, followed by increase of PTEN, and down-regulation of p-Akt. Collectively, these results suggest that formononetin exerts an anti-carcinogenic effect on BCa
in vitro
, which might be due to miR-21-mediated regulation of the PTEN/Akt pathway.
The isoflavone formononetin is the main active component of
Astragalus membranaceus
and possesses anti-tumorigenic properties. |
| doi_str_mv | 10.1039/c6fo01535b |
| format | Article |
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Astragalus membranaceus
and possesses anti-tumorigenic properties. However, the role of formononetin in human bladder cancer (BCa) has not been fully elucidated. The aim of the present study was to investigate the anti-tumor effects of formononetin on BCa cells and its potential molecular mechanism. T24 cells were treated with different concentrations of formononetin, and then the cell proliferation was assessed by MTT assay, cell apoptosis by Hoechst 33258 stain assay, cell invasiveness by transwell invasion assay, microRNA-21 (miR-21) expression by real-time PCR and the protein level of phosphatase and tensin homolog (PTEN) and phosphorylated homolog of Akt (p-Akt) by western blotting. The results showed that formononetin significantly inhibited the proliferation of T24 cells in a time- and dose-dependent manner. T24 cells treated with formononetin displayed obvious morphological changes of apoptosis and lower invasiveness. In addition, miR-21 expression was significantly decreased in formononetin-treated T24 cells, followed by increase of PTEN, and down-regulation of p-Akt. Collectively, these results suggest that formononetin exerts an anti-carcinogenic effect on BCa
in vitro
, which might be due to miR-21-mediated regulation of the PTEN/Akt pathway.
The isoflavone formononetin is the main active component of
Astragalus membranaceus
and possesses anti-tumorigenic properties.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/c6fo01535b</identifier><identifier>PMID: 28139790</identifier><language>eng</language><publisher>England</publisher><subject>Astragalus ; Astragalus Plant - chemistry ; Cell Proliferation - drug effects ; Drugs, Chinese Herbal - pharmacology ; Humans ; Isoflavones - pharmacology ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Neoplasm Invasiveness ; PTEN Phosphohydrolase - genetics ; PTEN Phosphohydrolase - metabolism ; Urinary Bladder Neoplasms - drug therapy ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - metabolism ; Urinary Bladder Neoplasms - physiopathology</subject><ispartof>Food & function, 2017-03, Vol.8 (3), p.161-166</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-e70dbf089f1619817796bc2fcc5e897814d92542bd6b71a767a5c256b0a67eed3</citedby><cites>FETCH-LOGICAL-c409t-e70dbf089f1619817796bc2fcc5e897814d92542bd6b71a767a5c256b0a67eed3</cites><orcidid>0000-0002-7025-436X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28139790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Yiying</creatorcontrib><creatorcontrib>Zhang, Xing</creatorcontrib><creatorcontrib>Li, Zhengzhao</creatorcontrib><creatorcontrib>Yan, Haibiao</creatorcontrib><creatorcontrib>Qin, Jian</creatorcontrib><creatorcontrib>Li, Tianyu</creatorcontrib><title>Formononetin inhibits human bladder cancer cell proliferation and invasiveness via regulation of miR-21 and PTEN</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>The isoflavone formononetin is the main active component of
Astragalus membranaceus
and possesses anti-tumorigenic properties. However, the role of formononetin in human bladder cancer (BCa) has not been fully elucidated. The aim of the present study was to investigate the anti-tumor effects of formononetin on BCa cells and its potential molecular mechanism. T24 cells were treated with different concentrations of formononetin, and then the cell proliferation was assessed by MTT assay, cell apoptosis by Hoechst 33258 stain assay, cell invasiveness by transwell invasion assay, microRNA-21 (miR-21) expression by real-time PCR and the protein level of phosphatase and tensin homolog (PTEN) and phosphorylated homolog of Akt (p-Akt) by western blotting. The results showed that formononetin significantly inhibited the proliferation of T24 cells in a time- and dose-dependent manner. T24 cells treated with formononetin displayed obvious morphological changes of apoptosis and lower invasiveness. In addition, miR-21 expression was significantly decreased in formononetin-treated T24 cells, followed by increase of PTEN, and down-regulation of p-Akt. Collectively, these results suggest that formononetin exerts an anti-carcinogenic effect on BCa
in vitro
, which might be due to miR-21-mediated regulation of the PTEN/Akt pathway.
The isoflavone formononetin is the main active component of
Astragalus membranaceus
and possesses anti-tumorigenic properties.</description><subject>Astragalus</subject><subject>Astragalus Plant - chemistry</subject><subject>Cell Proliferation - drug effects</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Humans</subject><subject>Isoflavones - pharmacology</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Neoplasm Invasiveness</subject><subject>PTEN Phosphohydrolase - genetics</subject><subject>PTEN Phosphohydrolase - metabolism</subject><subject>Urinary Bladder Neoplasms - drug therapy</subject><subject>Urinary Bladder Neoplasms - genetics</subject><subject>Urinary Bladder Neoplasms - metabolism</subject><subject>Urinary Bladder Neoplasms - physiopathology</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90ctKAzEUBuAgikrtxr0SdyKMJnPJZamlVUFUpIK7IVeNzCQ1mSn49k5t1Z3ZnMD5ckj-AHCI0TlGBb9QxAaEq6KSW2A_R2WekQq9bP_sS072wDildzSsgnPG2S7YyxkuOOVoHyxmIbbBB28656Hzb066LsG3vhUeykZobSJUwqtVMU0DFzE0zpooOhc8FF4Ph5YiuaXxJiW4dAJG89o3636wsHVPWY6_5eN8en8Adqxokhlv6gg8z6bzyU1293B9O7m8y1SJeJcZirS0iHGLCeYMU8qJVLlVqjKMU4ZLzfOqzKUmkmJBCRWVyisikSDUGF2MwOl67nDhj96krm5dWr1AeBP6VGPGhiwQxmSgZ2uqYkgpGlsvomtF_Kwxqlch1xMye_gO-WrAx5u5vWyN_qU_kQ7gZA1iUr_dv1-qF9oO5ug_U3wBQfuM9A</recordid><startdate>20170322</startdate><enddate>20170322</enddate><creator>Wu, Yiying</creator><creator>Zhang, Xing</creator><creator>Li, Zhengzhao</creator><creator>Yan, Haibiao</creator><creator>Qin, Jian</creator><creator>Li, Tianyu</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><orcidid>https://orcid.org/0000-0002-7025-436X</orcidid></search><sort><creationdate>20170322</creationdate><title>Formononetin inhibits human bladder cancer cell proliferation and invasiveness via regulation of miR-21 and PTEN</title><author>Wu, Yiying ; Zhang, Xing ; Li, Zhengzhao ; Yan, Haibiao ; Qin, Jian ; Li, Tianyu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-e70dbf089f1619817796bc2fcc5e897814d92542bd6b71a767a5c256b0a67eed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Astragalus</topic><topic>Astragalus Plant - chemistry</topic><topic>Cell Proliferation - drug effects</topic><topic>Drugs, Chinese Herbal - pharmacology</topic><topic>Humans</topic><topic>Isoflavones - pharmacology</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Neoplasm Invasiveness</topic><topic>PTEN Phosphohydrolase - genetics</topic><topic>PTEN Phosphohydrolase - metabolism</topic><topic>Urinary Bladder Neoplasms - drug therapy</topic><topic>Urinary Bladder Neoplasms - genetics</topic><topic>Urinary Bladder Neoplasms - metabolism</topic><topic>Urinary Bladder Neoplasms - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Yiying</creatorcontrib><creatorcontrib>Zhang, Xing</creatorcontrib><creatorcontrib>Li, Zhengzhao</creatorcontrib><creatorcontrib>Yan, Haibiao</creatorcontrib><creatorcontrib>Qin, Jian</creatorcontrib><creatorcontrib>Li, Tianyu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Yiying</au><au>Zhang, Xing</au><au>Li, Zhengzhao</au><au>Yan, Haibiao</au><au>Qin, Jian</au><au>Li, Tianyu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Formononetin inhibits human bladder cancer cell proliferation and invasiveness via regulation of miR-21 and PTEN</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2017-03-22</date><risdate>2017</risdate><volume>8</volume><issue>3</issue><spage>161</spage><epage>166</epage><pages>161-166</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>The isoflavone formononetin is the main active component of
Astragalus membranaceus
and possesses anti-tumorigenic properties. However, the role of formononetin in human bladder cancer (BCa) has not been fully elucidated. The aim of the present study was to investigate the anti-tumor effects of formononetin on BCa cells and its potential molecular mechanism. T24 cells were treated with different concentrations of formononetin, and then the cell proliferation was assessed by MTT assay, cell apoptosis by Hoechst 33258 stain assay, cell invasiveness by transwell invasion assay, microRNA-21 (miR-21) expression by real-time PCR and the protein level of phosphatase and tensin homolog (PTEN) and phosphorylated homolog of Akt (p-Akt) by western blotting. The results showed that formononetin significantly inhibited the proliferation of T24 cells in a time- and dose-dependent manner. T24 cells treated with formononetin displayed obvious morphological changes of apoptosis and lower invasiveness. In addition, miR-21 expression was significantly decreased in formononetin-treated T24 cells, followed by increase of PTEN, and down-regulation of p-Akt. Collectively, these results suggest that formononetin exerts an anti-carcinogenic effect on BCa
in vitro
, which might be due to miR-21-mediated regulation of the PTEN/Akt pathway.
The isoflavone formononetin is the main active component of
Astragalus membranaceus
and possesses anti-tumorigenic properties.</abstract><cop>England</cop><pmid>28139790</pmid><doi>10.1039/c6fo01535b</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-7025-436X</orcidid></addata></record> |
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| ispartof | Food & function, 2017-03, Vol.8 (3), p.161-166 |
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| source | MEDLINE; Royal Society Of Chemistry Journals 2008- |
| subjects | Astragalus Astragalus Plant - chemistry Cell Proliferation - drug effects Drugs, Chinese Herbal - pharmacology Humans Isoflavones - pharmacology MicroRNAs - genetics MicroRNAs - metabolism Neoplasm Invasiveness PTEN Phosphohydrolase - genetics PTEN Phosphohydrolase - metabolism Urinary Bladder Neoplasms - drug therapy Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - metabolism Urinary Bladder Neoplasms - physiopathology |
| title | Formononetin inhibits human bladder cancer cell proliferation and invasiveness via regulation of miR-21 and PTEN |
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