Synthesis of chitosan-coated polyoxometalate nanoparticles against cancer and its metastasis
Three different Keggin-type polyoxometalates (POMs) [PW 12 O 40 ] 3− , [TiW 11 CoO 40 ] 7− , and [Ti 2 PW 10 O 40 ] 7− , were synthesized and then encapsulated in chitosan to prepare nanoparticles, CS-PW 12 , CS-TiW 11 Co, and CS-Ti 2 PW 10 . The synthesized nanoparticles were physicochemically char...
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| Veröffentlicht in: | RSC advances 2015-01, Vol.5 (113), p.93234-93242 |
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| creator | Shah, Hamid Saeed Joshi, Sachin A Haider, Ali Kortz, Ulrich ur-Rehman, Nisar Iqbal, Jamshed |
| description | Three different Keggin-type polyoxometalates (POMs) [PW
12
O
40
]
3−
, [TiW
11
CoO
40
]
7−
, and [Ti
2
PW
10
O
40
]
7−
, were synthesized and then encapsulated in chitosan to prepare nanoparticles, CS-PW
12
, CS-TiW
11
Co, and CS-Ti
2
PW
10
. The synthesized nanoparticles were physicochemically characterized in terms of particle size, zeta potential, entrapment efficiency and
in vitro
release of the entrapped POM. The most efficient formulation was CS-TiW
11
Co, with a particle size of 105 ± 6 nm and an entrapment efficiency of 87 ± 12 (%). The CS-TiW
11
Co nanoparticles showed the highest activity when tested against tissue nonspecific alkaline phosphatase (TNAP) with IC
50
= 102.0 ± 9.68 nM. The anticancer potential of the free POMs and their nanoparticles were also studied and CS-TiW
11
Co showed the highest inhibition (IC
50
= 1.06 ± 0.09) on HeLa cells. To observe signs of apoptosis in HeLA cells, DAPI staining was performed after treatment with CS-TiW
11
Co nanoparticles. Furthermore, the reactive oxygen species (ROS) production was examined by H
2
DCF-DA dye under a fluorescence microscope. Our study revealed that CS-TiW
11
Co nanoparticles are very effective in cancer treatment and its associated metastasis especially in osteoblastic lesions with minimal adverse effects on normal cells (Vero cells).
HeLa cells, before and after treatment with nanoparticles. |
| doi_str_mv | 10.1039/c5ra18489d |
| format | Article |
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12
O
40
]
3−
, [TiW
11
CoO
40
]
7−
, and [Ti
2
PW
10
O
40
]
7−
, were synthesized and then encapsulated in chitosan to prepare nanoparticles, CS-PW
12
, CS-TiW
11
Co, and CS-Ti
2
PW
10
. The synthesized nanoparticles were physicochemically characterized in terms of particle size, zeta potential, entrapment efficiency and
in vitro
release of the entrapped POM. The most efficient formulation was CS-TiW
11
Co, with a particle size of 105 ± 6 nm and an entrapment efficiency of 87 ± 12 (%). The CS-TiW
11
Co nanoparticles showed the highest activity when tested against tissue nonspecific alkaline phosphatase (TNAP) with IC
50
= 102.0 ± 9.68 nM. The anticancer potential of the free POMs and their nanoparticles were also studied and CS-TiW
11
Co showed the highest inhibition (IC
50
= 1.06 ± 0.09) on HeLa cells. To observe signs of apoptosis in HeLA cells, DAPI staining was performed after treatment with CS-TiW
11
Co nanoparticles. Furthermore, the reactive oxygen species (ROS) production was examined by H
2
DCF-DA dye under a fluorescence microscope. Our study revealed that CS-TiW
11
Co nanoparticles are very effective in cancer treatment and its associated metastasis especially in osteoblastic lesions with minimal adverse effects on normal cells (Vero cells).
HeLa cells, before and after treatment with nanoparticles.</description><identifier>ISSN: 2046-2069</identifier><identifier>EISSN: 2046-2069</identifier><identifier>DOI: 10.1039/c5ra18489d</identifier><language>eng</language><ispartof>RSC advances, 2015-01, Vol.5 (113), p.93234-93242</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c279t-1cb012d679df4a3d36536d2cd3056dcd4b2b036865af1940206bf939b21d31b23</citedby><cites>FETCH-LOGICAL-c279t-1cb012d679df4a3d36536d2cd3056dcd4b2b036865af1940206bf939b21d31b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Shah, Hamid Saeed</creatorcontrib><creatorcontrib>Joshi, Sachin A</creatorcontrib><creatorcontrib>Haider, Ali</creatorcontrib><creatorcontrib>Kortz, Ulrich</creatorcontrib><creatorcontrib>ur-Rehman, Nisar</creatorcontrib><creatorcontrib>Iqbal, Jamshed</creatorcontrib><title>Synthesis of chitosan-coated polyoxometalate nanoparticles against cancer and its metastasis</title><title>RSC advances</title><description>Three different Keggin-type polyoxometalates (POMs) [PW
12
O
40
]
3−
, [TiW
11
CoO
40
]
7−
, and [Ti
2
PW
10
O
40
]
7−
, were synthesized and then encapsulated in chitosan to prepare nanoparticles, CS-PW
12
, CS-TiW
11
Co, and CS-Ti
2
PW
10
. The synthesized nanoparticles were physicochemically characterized in terms of particle size, zeta potential, entrapment efficiency and
in vitro
release of the entrapped POM. The most efficient formulation was CS-TiW
11
Co, with a particle size of 105 ± 6 nm and an entrapment efficiency of 87 ± 12 (%). The CS-TiW
11
Co nanoparticles showed the highest activity when tested against tissue nonspecific alkaline phosphatase (TNAP) with IC
50
= 102.0 ± 9.68 nM. The anticancer potential of the free POMs and their nanoparticles were also studied and CS-TiW
11
Co showed the highest inhibition (IC
50
= 1.06 ± 0.09) on HeLa cells. To observe signs of apoptosis in HeLA cells, DAPI staining was performed after treatment with CS-TiW
11
Co nanoparticles. Furthermore, the reactive oxygen species (ROS) production was examined by H
2
DCF-DA dye under a fluorescence microscope. Our study revealed that CS-TiW
11
Co nanoparticles are very effective in cancer treatment and its associated metastasis especially in osteoblastic lesions with minimal adverse effects on normal cells (Vero cells).
HeLa cells, before and after treatment with nanoparticles.</description><issn>2046-2069</issn><issn>2046-2069</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNp9kMtLAzEQh4MoWGov3oV4FVbz2E2bY6lWhYLg4yYss3nYyHazZHKw_71bK-rJYWB-DB8D8xFyytklZ1JfmSoBn5UzbQ_ISLBSFYIpffgnH5MJ4jsbSlVcKD4ir0_bLq8dBqTRU7MOOSJ0hYmQnaV9bLfxI25chnZY0A662EPKwbQOKbxB6DBTA51xiUJnachIdzQOHfCEHHlo0U2-55i8LG-eF3fF6uH2fjFfFUZMdS64aRgXVk219SVIK1UllRXGSlYpa2zZiIZJNVMVeK5LNnzSeC11I7iVvBFyTC72d02KiMn5uk9hA2lbc1bv1NSL6nH-peZ6gM_3cELzw_2qq3vrB-bsP0Z-AsQXbYE</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Shah, Hamid Saeed</creator><creator>Joshi, Sachin A</creator><creator>Haider, Ali</creator><creator>Kortz, Ulrich</creator><creator>ur-Rehman, Nisar</creator><creator>Iqbal, Jamshed</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20150101</creationdate><title>Synthesis of chitosan-coated polyoxometalate nanoparticles against cancer and its metastasis</title><author>Shah, Hamid Saeed ; Joshi, Sachin A ; Haider, Ali ; Kortz, Ulrich ; ur-Rehman, Nisar ; Iqbal, Jamshed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c279t-1cb012d679df4a3d36536d2cd3056dcd4b2b036865af1940206bf939b21d31b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shah, Hamid Saeed</creatorcontrib><creatorcontrib>Joshi, Sachin A</creatorcontrib><creatorcontrib>Haider, Ali</creatorcontrib><creatorcontrib>Kortz, Ulrich</creatorcontrib><creatorcontrib>ur-Rehman, Nisar</creatorcontrib><creatorcontrib>Iqbal, Jamshed</creatorcontrib><collection>CrossRef</collection><jtitle>RSC advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shah, Hamid Saeed</au><au>Joshi, Sachin A</au><au>Haider, Ali</au><au>Kortz, Ulrich</au><au>ur-Rehman, Nisar</au><au>Iqbal, Jamshed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis of chitosan-coated polyoxometalate nanoparticles against cancer and its metastasis</atitle><jtitle>RSC advances</jtitle><date>2015-01-01</date><risdate>2015</risdate><volume>5</volume><issue>113</issue><spage>93234</spage><epage>93242</epage><pages>93234-93242</pages><issn>2046-2069</issn><eissn>2046-2069</eissn><abstract>Three different Keggin-type polyoxometalates (POMs) [PW
12
O
40
]
3−
, [TiW
11
CoO
40
]
7−
, and [Ti
2
PW
10
O
40
]
7−
, were synthesized and then encapsulated in chitosan to prepare nanoparticles, CS-PW
12
, CS-TiW
11
Co, and CS-Ti
2
PW
10
. The synthesized nanoparticles were physicochemically characterized in terms of particle size, zeta potential, entrapment efficiency and
in vitro
release of the entrapped POM. The most efficient formulation was CS-TiW
11
Co, with a particle size of 105 ± 6 nm and an entrapment efficiency of 87 ± 12 (%). The CS-TiW
11
Co nanoparticles showed the highest activity when tested against tissue nonspecific alkaline phosphatase (TNAP) with IC
50
= 102.0 ± 9.68 nM. The anticancer potential of the free POMs and their nanoparticles were also studied and CS-TiW
11
Co showed the highest inhibition (IC
50
= 1.06 ± 0.09) on HeLa cells. To observe signs of apoptosis in HeLA cells, DAPI staining was performed after treatment with CS-TiW
11
Co nanoparticles. Furthermore, the reactive oxygen species (ROS) production was examined by H
2
DCF-DA dye under a fluorescence microscope. Our study revealed that CS-TiW
11
Co nanoparticles are very effective in cancer treatment and its associated metastasis especially in osteoblastic lesions with minimal adverse effects on normal cells (Vero cells).
HeLa cells, before and after treatment with nanoparticles.</abstract><doi>10.1039/c5ra18489d</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | RSC advances, 2015-01, Vol.5 (113), p.93234-93242 |
| issn | 2046-2069 2046-2069 |
| language | eng |
| recordid | cdi_crossref_primary_10_1039_C5RA18489D |
| source | Royal Society Of Chemistry Journals 2008- |
| title | Synthesis of chitosan-coated polyoxometalate nanoparticles against cancer and its metastasis |
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