Microfluidic immunocapture of circulating pancreatic cells using parallel EpCAM and MUC1 capture: characterization, optimization and downstream analysis

We have developed and optimized a microfluidic device platform for the capture and analysis of circulating pancreatic cells (CPCs) and pancreatic circulating tumor cells (CTCs). Our platform uses parallel anti-EpCAM and cancer-specific mucin 1 (MUC1) immunocapture in a silicon microdevice. Using a c...

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Veröffentlicht in:	Lab on a chip 2014-01, Vol.14 (1), p.1775-1784
Hauptverfasser:	Thege, Fredrik I, Lannin, Timothy B, Saha, Trisha N, Tsai, Shannon, Kochman, Michael L, Hollingsworth, Michael A, Rhim, Andrew D, Kirby, Brian J
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Immobilized - immunology Antigens, Neoplasm - analysis Antigens, Neoplasm - immunology Biomarkers, Tumor - analysis Biomarkers, Tumor - immunology Cell Adhesion Molecules - analysis Cell Adhesion Molecules - immunology Cell Line Epithelial Cell Adhesion Molecule Humans Indoles - analysis Indoles - immunology Islets of Langerhans - cytology Islets of Langerhans - metabolism Keratins - analysis Keratins - immunology Leukocyte Common Antigens - analysis Leukocyte Common Antigens - immunology Microfluidic Analytical Techniques - instrumentation Microfluidic Analytical Techniques - methods Mucin-1 - analysis Mucin-1 - immunology Mucin-4 - analysis Mucin-4 - immunology Neoplastic Cells, Circulating - metabolism Proto-Oncogene Proteins - blood Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - immunology Proto-Oncogene Proteins p21(ras) ras Proteins - blood ras Proteins - genetics ras Proteins - immunology RNA - blood RNA - isolation & purification Silicon - chemistry
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