A new sample preparation method for the absolute quantitation of a target proteome using 18 O labeling combined with multiple reaction monitoring mass spectrometry

A key step in the workflow of bottom-up proteomics is the proteolysis of proteins into peptides with trypsin. In addition, enzyme-catalytic 18 O labeled peptides as internal standards coupled with multiple reaction monitoring mass spectrometry (MRM MS) for the absolute quantitation of the target pro...

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Veröffentlicht in:	Analyst (London) 2015, Vol.140 (4), p.1281-1290
Hauptverfasser:	Li, Jiabin, Zhou, Lianqi, Wang, Huanhuan, Yan, Hui, Li, Nannan, Zhai, Rui, Jiao, Fenglong, Hao, Feiran, Jin, Zuyao, Tian, Fang, Peng, Bo, Zhang, Yangjun, Qian, Xiaohong
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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