PEG-conjugated highly dispersive multifunctional magnetic multi-walled carbon nanotubes for cellular imaging
We report synthesis of a highly versatile multicomponent nanosystem by covalently decorating the surface of multiwalled carbon nanotubes (CNTs) by magnetite nanoparticles (Fe 3 O 4 ), poly(ethylene glycol) (PEG), and fluorophore fluorescein isothiocyanate (FITC). The resulting Fe 3 O 4 PEGFITCCNT na...
Gespeichert in:
| Veröffentlicht in: | Nanoscale 2012-02, Vol.4 (3), p.837-844 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 844 |
|---|---|
| container_issue | 3 |
| container_start_page | 837 |
| container_title | Nanoscale |
| container_volume | 4 |
| creator | Khandare, Jayant J Jalota-Badhwar, Archana Satavalekar, Sneha D Bhansali, Sujit G Aher, Naval D Kharas, Firuza Banerjee, Shashwat S |
| description | We report synthesis of a highly versatile multicomponent nanosystem by covalently decorating the surface of multiwalled carbon nanotubes (CNTs) by magnetite nanoparticles (Fe
3
O
4
), poly(ethylene glycol) (PEG), and fluorophore fluorescein isothiocyanate (FITC). The resulting Fe
3
O
4
PEGFITCCNT nanosystem demonstrates high dispersion ability in an aqueous medium, magnetic responsiveness, and fluorescent capacity. Transmission electron microscopy images revealed that Fe
3
O
4
nanoparticles were well anchored onto the surfaces of the CNT.
In vitro
time kinetic experiments using confocal microscopy demonstrated a higher uptake of the Fe
3
O
4
PEGFITCCNT nanosystem localized at the perinuclear region of MCF7 cells compared to the free FITC. In addition, the CNT nanosystem demonstrated no evidence of toxicity on cell growth. Surface conjugation of multicomponents, combined with
in vitro
non-toxicity, enhanced cellular uptake for FITC and site specific targeting ability makes this fluorescent Fe
3
O
4
PEGFITCCNT nanosystem an ideal candidate for bioimaging, both
in vitro
and
in vivo
.
We present design, synthesis and
in vitro
evaluation of CNTs simultaneously conjugated with magnetite nanoparticles, PEG, and imaging probe. |
| doi_str_mv | 10.1039/c1nr11540e |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1039_C1NR11540E</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>22170574</sourcerecordid><originalsourceid>FETCH-LOGICAL-c334t-81355245a2f456ffd21eba41844215eb3f10e09496095e694c6e69f763e665133</originalsourceid><addsrcrecordid>eNp9kM9LwzAUx4Mobk4v3pV4Far53fUoY05hqIieS5omXUaWlqSd7L-3Wp03L-89-H7e4_EB4ByjG4xodquwDxhzhvQBGBPEUEJpSg73s2AjcBLjGiGRUUGPwYgQnCKesjFwL_NFomq_7irZ6hKubLVyO1ja2OgQ7VbDTedaazqvWlt76eBGVl63Vg1B8iGd6_eUDEXtoZe-brtCR2jqAJV2rnMyQNsvWV-dgiMjXdRnP30C3u_nb7OHZPm8eJzdLRNFKWuTKaacE8YlMYwLY0qCdSEZnjJGMNcFNRhplLFMoIxrkTEl-mpSQbUQHFM6AdfDXRXqGIM2eRP6F8Iuxyj_UpbP8NPrt7J5D18OcNMVG13u0V9HPXA1ACGqffrnPG9K0zMX_zH0E1A2fUA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>PEG-conjugated highly dispersive multifunctional magnetic multi-walled carbon nanotubes for cellular imaging</title><source>MEDLINE</source><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Khandare, Jayant J ; Jalota-Badhwar, Archana ; Satavalekar, Sneha D ; Bhansali, Sujit G ; Aher, Naval D ; Kharas, Firuza ; Banerjee, Shashwat S</creator><creatorcontrib>Khandare, Jayant J ; Jalota-Badhwar, Archana ; Satavalekar, Sneha D ; Bhansali, Sujit G ; Aher, Naval D ; Kharas, Firuza ; Banerjee, Shashwat S</creatorcontrib><description>We report synthesis of a highly versatile multicomponent nanosystem by covalently decorating the surface of multiwalled carbon nanotubes (CNTs) by magnetite nanoparticles (Fe
3
O
4
), poly(ethylene glycol) (PEG), and fluorophore fluorescein isothiocyanate (FITC). The resulting Fe
3
O
4
PEGFITCCNT nanosystem demonstrates high dispersion ability in an aqueous medium, magnetic responsiveness, and fluorescent capacity. Transmission electron microscopy images revealed that Fe
3
O
4
nanoparticles were well anchored onto the surfaces of the CNT.
In vitro
time kinetic experiments using confocal microscopy demonstrated a higher uptake of the Fe
3
O
4
PEGFITCCNT nanosystem localized at the perinuclear region of MCF7 cells compared to the free FITC. In addition, the CNT nanosystem demonstrated no evidence of toxicity on cell growth. Surface conjugation of multicomponents, combined with
in vitro
non-toxicity, enhanced cellular uptake for FITC and site specific targeting ability makes this fluorescent Fe
3
O
4
PEGFITCCNT nanosystem an ideal candidate for bioimaging, both
in vitro
and
in vivo
.
We present design, synthesis and
in vitro
evaluation of CNTs simultaneously conjugated with magnetite nanoparticles, PEG, and imaging probe.</description><identifier>ISSN: 2040-3364</identifier><identifier>EISSN: 2040-3372</identifier><identifier>DOI: 10.1039/c1nr11540e</identifier><identifier>PMID: 22170574</identifier><language>eng</language><publisher>England</publisher><subject>Breast Neoplasms - pathology ; Cell Line, Tumor ; Cell Survival ; Diagnostic Imaging - methods ; Female ; Fluorescein-5-isothiocyanate - chemistry ; Fluorescent Dyes - chemistry ; Humans ; Magnetite Nanoparticles - chemistry ; Magnetite Nanoparticles - ultrastructure ; Microscopy, Electron, Transmission ; Nanoconjugates - chemistry ; Nanoconjugates - ultrastructure ; Nanotechnology ; Nanotubes, Carbon - chemistry ; Nanotubes, Carbon - ultrastructure ; Optical Phenomena ; Polyethylene Glycols</subject><ispartof>Nanoscale, 2012-02, Vol.4 (3), p.837-844</ispartof><rights>This journal is © The Royal Society of Chemistry 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-81355245a2f456ffd21eba41844215eb3f10e09496095e694c6e69f763e665133</citedby><cites>FETCH-LOGICAL-c334t-81355245a2f456ffd21eba41844215eb3f10e09496095e694c6e69f763e665133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22170574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khandare, Jayant J</creatorcontrib><creatorcontrib>Jalota-Badhwar, Archana</creatorcontrib><creatorcontrib>Satavalekar, Sneha D</creatorcontrib><creatorcontrib>Bhansali, Sujit G</creatorcontrib><creatorcontrib>Aher, Naval D</creatorcontrib><creatorcontrib>Kharas, Firuza</creatorcontrib><creatorcontrib>Banerjee, Shashwat S</creatorcontrib><title>PEG-conjugated highly dispersive multifunctional magnetic multi-walled carbon nanotubes for cellular imaging</title><title>Nanoscale</title><addtitle>Nanoscale</addtitle><description>We report synthesis of a highly versatile multicomponent nanosystem by covalently decorating the surface of multiwalled carbon nanotubes (CNTs) by magnetite nanoparticles (Fe
3
O
4
), poly(ethylene glycol) (PEG), and fluorophore fluorescein isothiocyanate (FITC). The resulting Fe
3
O
4
PEGFITCCNT nanosystem demonstrates high dispersion ability in an aqueous medium, magnetic responsiveness, and fluorescent capacity. Transmission electron microscopy images revealed that Fe
3
O
4
nanoparticles were well anchored onto the surfaces of the CNT.
In vitro
time kinetic experiments using confocal microscopy demonstrated a higher uptake of the Fe
3
O
4
PEGFITCCNT nanosystem localized at the perinuclear region of MCF7 cells compared to the free FITC. In addition, the CNT nanosystem demonstrated no evidence of toxicity on cell growth. Surface conjugation of multicomponents, combined with
in vitro
non-toxicity, enhanced cellular uptake for FITC and site specific targeting ability makes this fluorescent Fe
3
O
4
PEGFITCCNT nanosystem an ideal candidate for bioimaging, both
in vitro
and
in vivo
.
We present design, synthesis and
in vitro
evaluation of CNTs simultaneously conjugated with magnetite nanoparticles, PEG, and imaging probe.</description><subject>Breast Neoplasms - pathology</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival</subject><subject>Diagnostic Imaging - methods</subject><subject>Female</subject><subject>Fluorescein-5-isothiocyanate - chemistry</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Humans</subject><subject>Magnetite Nanoparticles - chemistry</subject><subject>Magnetite Nanoparticles - ultrastructure</subject><subject>Microscopy, Electron, Transmission</subject><subject>Nanoconjugates - chemistry</subject><subject>Nanoconjugates - ultrastructure</subject><subject>Nanotechnology</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Nanotubes, Carbon - ultrastructure</subject><subject>Optical Phenomena</subject><subject>Polyethylene Glycols</subject><issn>2040-3364</issn><issn>2040-3372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9LwzAUx4Mobk4v3pV4Far53fUoY05hqIieS5omXUaWlqSd7L-3Wp03L-89-H7e4_EB4ByjG4xodquwDxhzhvQBGBPEUEJpSg73s2AjcBLjGiGRUUGPwYgQnCKesjFwL_NFomq_7irZ6hKubLVyO1ja2OgQ7VbDTedaazqvWlt76eBGVl63Vg1B8iGd6_eUDEXtoZe-brtCR2jqAJV2rnMyQNsvWV-dgiMjXdRnP30C3u_nb7OHZPm8eJzdLRNFKWuTKaacE8YlMYwLY0qCdSEZnjJGMNcFNRhplLFMoIxrkTEl-mpSQbUQHFM6AdfDXRXqGIM2eRP6F8Iuxyj_UpbP8NPrt7J5D18OcNMVG13u0V9HPXA1ACGqffrnPG9K0zMX_zH0E1A2fUA</recordid><startdate>20120207</startdate><enddate>20120207</enddate><creator>Khandare, Jayant J</creator><creator>Jalota-Badhwar, Archana</creator><creator>Satavalekar, Sneha D</creator><creator>Bhansali, Sujit G</creator><creator>Aher, Naval D</creator><creator>Kharas, Firuza</creator><creator>Banerjee, Shashwat S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20120207</creationdate><title>PEG-conjugated highly dispersive multifunctional magnetic multi-walled carbon nanotubes for cellular imaging</title><author>Khandare, Jayant J ; Jalota-Badhwar, Archana ; Satavalekar, Sneha D ; Bhansali, Sujit G ; Aher, Naval D ; Kharas, Firuza ; Banerjee, Shashwat S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-81355245a2f456ffd21eba41844215eb3f10e09496095e694c6e69f763e665133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Breast Neoplasms - pathology</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival</topic><topic>Diagnostic Imaging - methods</topic><topic>Female</topic><topic>Fluorescein-5-isothiocyanate - chemistry</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Humans</topic><topic>Magnetite Nanoparticles - chemistry</topic><topic>Magnetite Nanoparticles - ultrastructure</topic><topic>Microscopy, Electron, Transmission</topic><topic>Nanoconjugates - chemistry</topic><topic>Nanoconjugates - ultrastructure</topic><topic>Nanotechnology</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Nanotubes, Carbon - ultrastructure</topic><topic>Optical Phenomena</topic><topic>Polyethylene Glycols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Khandare, Jayant J</creatorcontrib><creatorcontrib>Jalota-Badhwar, Archana</creatorcontrib><creatorcontrib>Satavalekar, Sneha D</creatorcontrib><creatorcontrib>Bhansali, Sujit G</creatorcontrib><creatorcontrib>Aher, Naval D</creatorcontrib><creatorcontrib>Kharas, Firuza</creatorcontrib><creatorcontrib>Banerjee, Shashwat S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Nanoscale</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khandare, Jayant J</au><au>Jalota-Badhwar, Archana</au><au>Satavalekar, Sneha D</au><au>Bhansali, Sujit G</au><au>Aher, Naval D</au><au>Kharas, Firuza</au><au>Banerjee, Shashwat S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PEG-conjugated highly dispersive multifunctional magnetic multi-walled carbon nanotubes for cellular imaging</atitle><jtitle>Nanoscale</jtitle><addtitle>Nanoscale</addtitle><date>2012-02-07</date><risdate>2012</risdate><volume>4</volume><issue>3</issue><spage>837</spage><epage>844</epage><pages>837-844</pages><issn>2040-3364</issn><eissn>2040-3372</eissn><abstract>We report synthesis of a highly versatile multicomponent nanosystem by covalently decorating the surface of multiwalled carbon nanotubes (CNTs) by magnetite nanoparticles (Fe
3
O
4
), poly(ethylene glycol) (PEG), and fluorophore fluorescein isothiocyanate (FITC). The resulting Fe
3
O
4
PEGFITCCNT nanosystem demonstrates high dispersion ability in an aqueous medium, magnetic responsiveness, and fluorescent capacity. Transmission electron microscopy images revealed that Fe
3
O
4
nanoparticles were well anchored onto the surfaces of the CNT.
In vitro
time kinetic experiments using confocal microscopy demonstrated a higher uptake of the Fe
3
O
4
PEGFITCCNT nanosystem localized at the perinuclear region of MCF7 cells compared to the free FITC. In addition, the CNT nanosystem demonstrated no evidence of toxicity on cell growth. Surface conjugation of multicomponents, combined with
in vitro
non-toxicity, enhanced cellular uptake for FITC and site specific targeting ability makes this fluorescent Fe
3
O
4
PEGFITCCNT nanosystem an ideal candidate for bioimaging, both
in vitro
and
in vivo
.
We present design, synthesis and
in vitro
evaluation of CNTs simultaneously conjugated with magnetite nanoparticles, PEG, and imaging probe.</abstract><cop>England</cop><pmid>22170574</pmid><doi>10.1039/c1nr11540e</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2040-3364 |
| ispartof | Nanoscale, 2012-02, Vol.4 (3), p.837-844 |
| issn | 2040-3364 2040-3372 |
| language | eng |
| recordid | cdi_crossref_primary_10_1039_C1NR11540E |
| source | MEDLINE; Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection |
| subjects | Breast Neoplasms - pathology Cell Line, Tumor Cell Survival Diagnostic Imaging - methods Female Fluorescein-5-isothiocyanate - chemistry Fluorescent Dyes - chemistry Humans Magnetite Nanoparticles - chemistry Magnetite Nanoparticles - ultrastructure Microscopy, Electron, Transmission Nanoconjugates - chemistry Nanoconjugates - ultrastructure Nanotechnology Nanotubes, Carbon - chemistry Nanotubes, Carbon - ultrastructure Optical Phenomena Polyethylene Glycols |
| title | PEG-conjugated highly dispersive multifunctional magnetic multi-walled carbon nanotubes for cellular imaging |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T00%3A38%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=PEG-conjugated%20highly%20dispersive%20multifunctional%20magnetic%20multi-walled%20carbon%20nanotubes%20for%20cellular%20imaging&rft.jtitle=Nanoscale&rft.au=Khandare,%20Jayant%20J&rft.date=2012-02-07&rft.volume=4&rft.issue=3&rft.spage=837&rft.epage=844&rft.pages=837-844&rft.issn=2040-3364&rft.eissn=2040-3372&rft_id=info:doi/10.1039/c1nr11540e&rft_dat=%3Cpubmed_cross%3E22170574%3C/pubmed_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/22170574&rfr_iscdi=true |