Whole Genome Expression in Newborn Mouse Brain Tissue after Hypoxia and Reoxygenation
Supplementary oxygen during resuscitation can be harmful to the immature brain and lead to both acute and long-term morbidity. The aim was to study whole genome expression alterations induced by supplementary oxygen in the brain in a newborn mouse model of hypoxia and reoxygenation. C57BL/6 mice (n...
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| Veröffentlicht in: | Pediatric research 2011-11, Vol.70 (Suppl 5), p.223-223 |
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| creator | Wollen, E J Wright, M S Gunther, C -C Bik-Multanowski, M Madetko-Talowska, A Sejersted, Y Kwinta, P Pietrzyk, J J Saugstad, O D |
| description | Supplementary oxygen during resuscitation can be harmful to the immature brain and lead to both acute and long-term morbidity. The aim was to study whole genome expression alterations induced by supplementary oxygen in the brain in a newborn mouse model of hypoxia and reoxygenation.
C57BL/6 mice (n =41) postnatal day 7 were randomized to hypoxia (8% O
2
, 36±0.5C°) for 120 min and reoxygenation for 30 min in 21% (H21, n=9), 40% (H40, n= 10), 60% (H60, n= 11) or 100% (H100, n= 11) O
2
for 30 min. After 150 min recovery in room air, mice were sacrificed and brain tissue harvested. The control group (C21, n = 9) was exposed to 21% O
2
for 300 min. Qiagen RNeasy kit was used for mRNA extraction. GeneChip Mouse Gene ST 1.0 Arrays (Affymetrix) were used to analyze whole genome expression. Statistical analyses were performed by using BAMarray Software. GSEA was used for pathway analyses.
458 genes were significantly differentially expressed in brain tissue in the comparison between C21 and the 4 hypoxia groups (H21: 32, H40: 7, H60: 117, H100: 302). 91.5% of the genes are represented in H60 and H100 groups. 40 genes are overlapping between group H60 and H100. Oxidative phosphorylation is one pathway that is significantly down regulated (FDR q-value< 0.10) for both H60 and H100.
91.5 % of the genes differentially expressed are represented in the groups resuscitated with either 60 or 100% O
2
. Hypoxia followed by hyperoxia induces the down regulation of oxidative phosphorylation in the current model. |
| doi_str_mv | 10.1038/pr.2011.448 |
| format | Article |
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C57BL/6 mice (n =41) postnatal day 7 were randomized to hypoxia (8% O
2
, 36±0.5C°) for 120 min and reoxygenation for 30 min in 21% (H21, n=9), 40% (H40, n= 10), 60% (H60, n= 11) or 100% (H100, n= 11) O
2
for 30 min. After 150 min recovery in room air, mice were sacrificed and brain tissue harvested. The control group (C21, n = 9) was exposed to 21% O
2
for 300 min. Qiagen RNeasy kit was used for mRNA extraction. GeneChip Mouse Gene ST 1.0 Arrays (Affymetrix) were used to analyze whole genome expression. Statistical analyses were performed by using BAMarray Software. GSEA was used for pathway analyses.
458 genes were significantly differentially expressed in brain tissue in the comparison between C21 and the 4 hypoxia groups (H21: 32, H40: 7, H60: 117, H100: 302). 91.5% of the genes are represented in H60 and H100 groups. 40 genes are overlapping between group H60 and H100. Oxidative phosphorylation is one pathway that is significantly down regulated (FDR q-value< 0.10) for both H60 and H100.
91.5 % of the genes differentially expressed are represented in the groups resuscitated with either 60 or 100% O
2
. Hypoxia followed by hyperoxia induces the down regulation of oxidative phosphorylation in the current model.</description><identifier>ISSN: 0031-3998</identifier><identifier>EISSN: 1530-0447</identifier><identifier>DOI: 10.1038/pr.2011.448</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>631/1647/2217/2218 ; 631/1647/334 ; 692/698/1688/64 ; 692/700/1720 ; Medicine ; Medicine & Public Health ; Pediatric Surgery ; Pediatrics ; poster-presentations-brain-nervous-system</subject><ispartof>Pediatric research, 2011-11, Vol.70 (Suppl 5), p.223-223</ispartof><rights>International Pediatrics Research Foundation, Inc. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids></links><search><creatorcontrib>Wollen, E J</creatorcontrib><creatorcontrib>Wright, M S</creatorcontrib><creatorcontrib>Gunther, C -C</creatorcontrib><creatorcontrib>Bik-Multanowski, M</creatorcontrib><creatorcontrib>Madetko-Talowska, A</creatorcontrib><creatorcontrib>Sejersted, Y</creatorcontrib><creatorcontrib>Kwinta, P</creatorcontrib><creatorcontrib>Pietrzyk, J J</creatorcontrib><creatorcontrib>Saugstad, O D</creatorcontrib><title>Whole Genome Expression in Newborn Mouse Brain Tissue after Hypoxia and Reoxygenation</title><title>Pediatric research</title><addtitle>Pediatr Res</addtitle><description>Supplementary oxygen during resuscitation can be harmful to the immature brain and lead to both acute and long-term morbidity. The aim was to study whole genome expression alterations induced by supplementary oxygen in the brain in a newborn mouse model of hypoxia and reoxygenation.
C57BL/6 mice (n =41) postnatal day 7 were randomized to hypoxia (8% O
2
, 36±0.5C°) for 120 min and reoxygenation for 30 min in 21% (H21, n=9), 40% (H40, n= 10), 60% (H60, n= 11) or 100% (H100, n= 11) O
2
for 30 min. After 150 min recovery in room air, mice were sacrificed and brain tissue harvested. The control group (C21, n = 9) was exposed to 21% O
2
for 300 min. Qiagen RNeasy kit was used for mRNA extraction. GeneChip Mouse Gene ST 1.0 Arrays (Affymetrix) were used to analyze whole genome expression. Statistical analyses were performed by using BAMarray Software. GSEA was used for pathway analyses.
458 genes were significantly differentially expressed in brain tissue in the comparison between C21 and the 4 hypoxia groups (H21: 32, H40: 7, H60: 117, H100: 302). 91.5% of the genes are represented in H60 and H100 groups. 40 genes are overlapping between group H60 and H100. Oxidative phosphorylation is one pathway that is significantly down regulated (FDR q-value< 0.10) for both H60 and H100.
91.5 % of the genes differentially expressed are represented in the groups resuscitated with either 60 or 100% O
2
. Hypoxia followed by hyperoxia induces the down regulation of oxidative phosphorylation in the current model.</description><subject>631/1647/2217/2218</subject><subject>631/1647/334</subject><subject>692/698/1688/64</subject><subject>692/700/1720</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Pediatric Surgery</subject><subject>Pediatrics</subject><subject>poster-presentations-brain-nervous-system</subject><issn>0031-3998</issn><issn>1530-0447</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><recordid>eNptkE1LAzEQhoMoWKsn_0DuunVmN-kmRy39EKqCtHhcsruzdUubLEmL7b83pR49DTM88_LyMHaPMEDI1FPnBykgDoRQF6yHMoMEhMgvWQ8gwyTTWl2zmxDWACikEj22_Pp2G-JTsm5LfHzoPIXQOstby9_pp3Te8je3D8RfvIm3RRvCnrhpduT57Ni5Q2u4sTX_JHc4rsiaXfy-ZVeN2QS6-5t9tpyMF6NZMv-Yvo6e50mFCCqR0JS5QDSkmxQEaqpMraSRMhUalarLWqoq10OtMpRpriArU52n1OihwbzO-uzhnFt5F4Knpuh8uzX-WCAUJyNxL05Gimgk0o9nOkTKrsgXa7f3Nhb8F_8Feqlh5A</recordid><startdate>201111</startdate><enddate>201111</enddate><creator>Wollen, E J</creator><creator>Wright, M S</creator><creator>Gunther, C -C</creator><creator>Bik-Multanowski, M</creator><creator>Madetko-Talowska, A</creator><creator>Sejersted, Y</creator><creator>Kwinta, P</creator><creator>Pietrzyk, J J</creator><creator>Saugstad, O D</creator><general>Nature Publishing Group US</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>201111</creationdate><title>Whole Genome Expression in Newborn Mouse Brain Tissue after Hypoxia and Reoxygenation</title><author>Wollen, E J ; Wright, M S ; Gunther, C -C ; Bik-Multanowski, M ; Madetko-Talowska, A ; Sejersted, Y ; Kwinta, P ; Pietrzyk, J J ; Saugstad, O D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1108-50fb7411ae9f20419ecad85a55249188dbd58c7969831527803b2972ef96a17d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>631/1647/2217/2218</topic><topic>631/1647/334</topic><topic>692/698/1688/64</topic><topic>692/700/1720</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Pediatric Surgery</topic><topic>Pediatrics</topic><topic>poster-presentations-brain-nervous-system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wollen, E J</creatorcontrib><creatorcontrib>Wright, M S</creatorcontrib><creatorcontrib>Gunther, C -C</creatorcontrib><creatorcontrib>Bik-Multanowski, M</creatorcontrib><creatorcontrib>Madetko-Talowska, A</creatorcontrib><creatorcontrib>Sejersted, Y</creatorcontrib><creatorcontrib>Kwinta, P</creatorcontrib><creatorcontrib>Pietrzyk, J J</creatorcontrib><creatorcontrib>Saugstad, O D</creatorcontrib><collection>CrossRef</collection><jtitle>Pediatric research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wollen, E J</au><au>Wright, M S</au><au>Gunther, C -C</au><au>Bik-Multanowski, M</au><au>Madetko-Talowska, A</au><au>Sejersted, Y</au><au>Kwinta, P</au><au>Pietrzyk, J J</au><au>Saugstad, O D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Whole Genome Expression in Newborn Mouse Brain Tissue after Hypoxia and Reoxygenation</atitle><jtitle>Pediatric research</jtitle><stitle>Pediatr Res</stitle><date>2011-11</date><risdate>2011</risdate><volume>70</volume><issue>Suppl 5</issue><spage>223</spage><epage>223</epage><pages>223-223</pages><issn>0031-3998</issn><eissn>1530-0447</eissn><abstract>Supplementary oxygen during resuscitation can be harmful to the immature brain and lead to both acute and long-term morbidity. The aim was to study whole genome expression alterations induced by supplementary oxygen in the brain in a newborn mouse model of hypoxia and reoxygenation.
C57BL/6 mice (n =41) postnatal day 7 were randomized to hypoxia (8% O
2
, 36±0.5C°) for 120 min and reoxygenation for 30 min in 21% (H21, n=9), 40% (H40, n= 10), 60% (H60, n= 11) or 100% (H100, n= 11) O
2
for 30 min. After 150 min recovery in room air, mice were sacrificed and brain tissue harvested. The control group (C21, n = 9) was exposed to 21% O
2
for 300 min. Qiagen RNeasy kit was used for mRNA extraction. GeneChip Mouse Gene ST 1.0 Arrays (Affymetrix) were used to analyze whole genome expression. Statistical analyses were performed by using BAMarray Software. GSEA was used for pathway analyses.
458 genes were significantly differentially expressed in brain tissue in the comparison between C21 and the 4 hypoxia groups (H21: 32, H40: 7, H60: 117, H100: 302). 91.5% of the genes are represented in H60 and H100 groups. 40 genes are overlapping between group H60 and H100. Oxidative phosphorylation is one pathway that is significantly down regulated (FDR q-value< 0.10) for both H60 and H100.
91.5 % of the genes differentially expressed are represented in the groups resuscitated with either 60 or 100% O
2
. Hypoxia followed by hyperoxia induces the down regulation of oxidative phosphorylation in the current model.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><doi>10.1038/pr.2011.448</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/1647/2217/2218 631/1647/334 692/698/1688/64 692/700/1720 Medicine Medicine & Public Health Pediatric Surgery Pediatrics poster-presentations-brain-nervous-system |
| title | Whole Genome Expression in Newborn Mouse Brain Tissue after Hypoxia and Reoxygenation |
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