Rapid, directional RNA-seq library preparation kits for formalin-fixed paraffin-embedded RNA

Preparing RNA-seq libraries using RNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue is notoriously difficult, due to limited sample amounts and RNA degradation. Here we describe the ScriptSeq™ Complete Kits, which enable researchers to go from as little as 100 ng of total FFPE RNA to directional, cluster-ready RNA-seq libraries in 1 d. The libraries are virtually free of contaminating ribosomal RNA (rRNA) and exhibit high quality scores and percentage of mapped reads.