Somatic seeds: encapsulation of asexual plant embryos
Somatic embryos (SE) of alfalfa, celery, and cauliflower were encapsulated as single–embryo beads approximately four millimeters in diameter to produce individual somatic (artificial) seeds. The SE were mixed in sodium alginate and dropped into a solution of calcium chloride to form calcium alginate...
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| Veröffentlicht in: | Bio/Technology 1986-09, Vol.4 (9), p.797-801 |
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| creator | Redenbaugh, Keith Paasch, Brian D. Nichol, James W. Kossler, Mary E. Viss, Peter R. Walker, Keith A. |
| description | Somatic embryos (SE) of alfalfa, celery, and cauliflower were encapsulated as single–embryo beads approximately four millimeters in diameter to produce individual somatic (artificial) seeds. The SE were mixed in sodium alginate and dropped into a solution of calcium chloride to form calcium alginate beads via an ion exchange reaction. For our two best somatic embryogenesis systems, alfalfa and celery,
in vitro
germination frequencies with production of seedling–quality plants were routinely 30% and 65%, respectively. In addition, encapsulated alfalfa and celery SE developed into plants in sand trays or in transplant plugs at frequencies of 7% and 10%. The production of seedling–quality plants from individual somatic seeds, as first reported here, has potential as a novel, universal delivery system. |
| doi_str_mv | 10.1038/nbt0986-797 |
| format | Article |
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in vitro
germination frequencies with production of seedling–quality plants were routinely 30% and 65%, respectively. In addition, encapsulated alfalfa and celery SE developed into plants in sand trays or in transplant plugs at frequencies of 7% and 10%. The production of seedling–quality plants from individual somatic seeds, as first reported here, has potential as a novel, universal delivery system.</description><identifier>ISSN: 0733-222X</identifier><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 2331-3684</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt0986-797</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Agriculture ; Agronomy ; Agronomy. Soil science and plant productions ; apium graveolens ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; brassica ; desarrollo embrionario ; developpement embryonnaire ; embrion ; embryo ; embryon ; embryonic development ; Fundamental and applied biological sciences. Psychology ; Genetic engineering applications ; Genetics and breeding of economic plants ; germinacion ; germination ; graine ; Industrial applications and implications. Economical aspects ; Life Sciences ; medicago sativa ; Plant breeding: fundamental aspects and methodology ; research-paper ; seeds ; Seeds and other planting stocks ; semilla</subject><ispartof>Bio/Technology, 1986-09, Vol.4 (9), p.797-801</ispartof><rights>Nature Publishing Company 1986</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c351t-62eb594e4064e9eed37fd7f3153216e3e88a128e4ed50349546e7d38b4a94a6a3</citedby><cites>FETCH-LOGICAL-c351t-62eb594e4064e9eed37fd7f3153216e3e88a128e4ed50349546e7d38b4a94a6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nbt0986-797$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nbt0986-797$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8083794$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8239796$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Redenbaugh, Keith</creatorcontrib><creatorcontrib>Paasch, Brian D.</creatorcontrib><creatorcontrib>Nichol, James W.</creatorcontrib><creatorcontrib>Kossler, Mary E.</creatorcontrib><creatorcontrib>Viss, Peter R.</creatorcontrib><creatorcontrib>Walker, Keith A.</creatorcontrib><creatorcontrib>Eidg. Technische Hochschule, Zurich (Switzerland)</creatorcontrib><title>Somatic seeds: encapsulation of asexual plant embryos</title><title>Bio/Technology</title><addtitle>Nat Biotechnol</addtitle><description>Somatic embryos (SE) of alfalfa, celery, and cauliflower were encapsulated as single–embryo beads approximately four millimeters in diameter to produce individual somatic (artificial) seeds. The SE were mixed in sodium alginate and dropped into a solution of calcium chloride to form calcium alginate beads via an ion exchange reaction. For our two best somatic embryogenesis systems, alfalfa and celery,
in vitro
germination frequencies with production of seedling–quality plants were routinely 30% and 65%, respectively. In addition, encapsulated alfalfa and celery SE developed into plants in sand trays or in transplant plugs at frequencies of 7% and 10%. The production of seedling–quality plants from individual somatic seeds, as first reported here, has potential as a novel, universal delivery system.</description><subject>Agriculture</subject><subject>Agronomy</subject><subject>Agronomy. Soil science and plant productions</subject><subject>apium graveolens</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>brassica</subject><subject>desarrollo embrionario</subject><subject>developpement embryonnaire</subject><subject>embrion</subject><subject>embryo</subject><subject>embryon</subject><subject>embryonic development</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering applications</subject><subject>Genetics and breeding of economic plants</subject><subject>germinacion</subject><subject>germination</subject><subject>graine</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Life Sciences</subject><subject>medicago sativa</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>research-paper</subject><subject>seeds</subject><subject>Seeds and other planting stocks</subject><subject>semilla</subject><issn>0733-222X</issn><issn>1087-0156</issn><issn>2331-3684</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><recordid>eNqFkU1Lw0AQhhdRsFZP3iUHbxrd3dnshzcpfkHBQy14C5NkUlrSJOymYP-9KynexNPAzDPv8DCMXQp-JzjY-7YYuLM6Nc4csYkEECloq47ZhBuAVEr5ecrOQthwroyWasKyRbfFYV0mgagKDwm1JfZh18Re1yZdnWCgrx02Sd9gOyS0Lfy-C-fspMYm0MWhTtny-elj9prO31_eZo_ztIRMDKmWVGROkeJakYsHwNSVqUFkIIUmIGtRSEuKqoyDcpnSZCqwhUKnUCNM2c2YW_ouBE913vv1Fv0-Fzz_Mc4Pxnk0jvT1SPcYSmxqj225Dr8rVoIzTv-LcQvGqYjdjliIk3ZFPt90O99G3T-OX414jV2OKx8TlwvhrOE8kxBf8Q3mtXnR</recordid><startdate>19860901</startdate><enddate>19860901</enddate><creator>Redenbaugh, Keith</creator><creator>Paasch, Brian D.</creator><creator>Nichol, James W.</creator><creator>Kossler, Mary E.</creator><creator>Viss, Peter R.</creator><creator>Walker, Keith A.</creator><general>Nature Publishing Group US</general><general>Nature Publications</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19860901</creationdate><title>Somatic seeds: encapsulation of asexual plant embryos</title><author>Redenbaugh, Keith ; Paasch, Brian D. ; Nichol, James W. ; Kossler, Mary E. ; Viss, Peter R. ; Walker, Keith A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c351t-62eb594e4064e9eed37fd7f3153216e3e88a128e4ed50349546e7d38b4a94a6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>Agriculture</topic><topic>Agronomy</topic><topic>Agronomy. Soil science and plant productions</topic><topic>apium graveolens</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>brassica</topic><topic>desarrollo embrionario</topic><topic>developpement embryonnaire</topic><topic>embrion</topic><topic>embryo</topic><topic>embryon</topic><topic>embryonic development</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering applications</topic><topic>Genetics and breeding of economic plants</topic><topic>germinacion</topic><topic>germination</topic><topic>graine</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Life Sciences</topic><topic>medicago sativa</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>research-paper</topic><topic>seeds</topic><topic>Seeds and other planting stocks</topic><topic>semilla</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Redenbaugh, Keith</creatorcontrib><creatorcontrib>Paasch, Brian D.</creatorcontrib><creatorcontrib>Nichol, James W.</creatorcontrib><creatorcontrib>Kossler, Mary E.</creatorcontrib><creatorcontrib>Viss, Peter R.</creatorcontrib><creatorcontrib>Walker, Keith A.</creatorcontrib><creatorcontrib>Eidg. Technische Hochschule, Zurich (Switzerland)</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Bio/Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Redenbaugh, Keith</au><au>Paasch, Brian D.</au><au>Nichol, James W.</au><au>Kossler, Mary E.</au><au>Viss, Peter R.</au><au>Walker, Keith A.</au><aucorp>Eidg. Technische Hochschule, Zurich (Switzerland)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Somatic seeds: encapsulation of asexual plant embryos</atitle><jtitle>Bio/Technology</jtitle><stitle>Nat Biotechnol</stitle><date>1986-09-01</date><risdate>1986</risdate><volume>4</volume><issue>9</issue><spage>797</spage><epage>801</epage><pages>797-801</pages><issn>0733-222X</issn><issn>1087-0156</issn><eissn>2331-3684</eissn><eissn>1546-1696</eissn><abstract>Somatic embryos (SE) of alfalfa, celery, and cauliflower were encapsulated as single–embryo beads approximately four millimeters in diameter to produce individual somatic (artificial) seeds. The SE were mixed in sodium alginate and dropped into a solution of calcium chloride to form calcium alginate beads via an ion exchange reaction. For our two best somatic embryogenesis systems, alfalfa and celery,
in vitro
germination frequencies with production of seedling–quality plants were routinely 30% and 65%, respectively. In addition, encapsulated alfalfa and celery SE developed into plants in sand trays or in transplant plugs at frequencies of 7% and 10%. The production of seedling–quality plants from individual somatic seeds, as first reported here, has potential as a novel, universal delivery system.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><doi>10.1038/nbt0986-797</doi><tpages>5</tpages></addata></record> |
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| source | Springer Nature - Complete Springer Journals; Nature |
| subjects | Agriculture Agronomy Agronomy. Soil science and plant productions apium graveolens Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology brassica desarrollo embrionario developpement embryonnaire embrion embryo embryon embryonic development Fundamental and applied biological sciences. Psychology Genetic engineering applications Genetics and breeding of economic plants germinacion germination graine Industrial applications and implications. Economical aspects Life Sciences medicago sativa Plant breeding: fundamental aspects and methodology research-paper seeds Seeds and other planting stocks semilla |
| title | Somatic seeds: encapsulation of asexual plant embryos |
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