A glucose-derepressed promoter for expression of heterologous products in the filamentous fungus Aspergillus nidulans

We describe a putative binding sequence (GCGGGGC) for the glucose-responsive represser protein CreA at two positions upstream of the transcription start site of the alcohol dehydrogenase I ( alc A) gene of Aspergillus nidulans . To positively identify the putative binding sites as CreA-specifie, the...

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Veröffentlicht in:	Bio/Technology 1993-07, Vol.11 (7), p.815-818
Hauptverfasser:	Hintz, W.E, Lagosky, P.A
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN ADN RECOMBINADO ADN RECOMBINE Agriculture ALCOHOL DEHYDROGENASE ALCOHOL DESHIDROGENASA ALCOOL DESHYDROGENASE Amino Acid Sequence ASPERGILLUS NIDULANS Base Sequence BINDING SITE Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology CREA PROTEIN DNA DNA BINDING PROTEINS EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics GENE GENE EXPRESSION Gene Expression Regulation, Fungal - physiology GENES Genetic engineering GENETIC REGULATION Genetic technics GENETIC TRANSFORMATION GENETICA GENETICS GENETIQUE GLUCOSA GLUCOSE Glucose - physiology Life Sciences Methods. Procedures. Technologies Modification of gene expression level Molecular Sequence Data Mutagenesis, Site-Directed Promoter Regions, Genetic PROTEINAS PROTEINE PROTEINS RECOMBINANT DNA Repressor Proteins - genetics Selection, Genetic STRUCTURAL GENES TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transformation, Genetic
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