Molecular Cloning and Synthesis of Biologically Active Human Tissue Inhibitor of Metalloproteinases in Yeast
Tissue inhibitor of metalloproteinases (TIMP) is a widely distributed glycoprotein that stochiometrically inactivates metalloproteinases involved in connective tissue catabolism. Here we report the cDNA cloning of TIMP from human fibroblastic MRC5 cells using a single 42–base oligonucleotide probe....
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| Veröffentlicht in: | Bio/Technology 1987-06, Vol.5 (6), p.595-598 |
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| container_issue | 6 |
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| container_title | Bio/Technology |
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| creator | Kaczorek, Michel Honoré, Nadine Ribes, Véronique Dehoux, Pierre Cornet, Philippe Cartwright, Terence Streeck, Rolf E. |
| description | Tissue inhibitor of metalloproteinases (TIMP) is a widely distributed glycoprotein that stochiometrically inactivates metalloproteinases involved in connective tissue catabolism. Here we report the cDNA cloning of TIMP from human fibroblastic MRC5 cells using a single 42–base oligonucleotide probe. Expression in
S. cerevisiae
of complete TIMP cDNA yielded insoluble protein aggregates. Biologically active TIMP was reconstituted from the yeast product by a denaturation/renaturation procedure. |
| doi_str_mv | 10.1038/nbt0687-595 |
| format | Article |
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S. cerevisiae
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S. cerevisiae
of complete TIMP cDNA yielded insoluble protein aggregates. Biologically active TIMP was reconstituted from the yeast product by a denaturation/renaturation procedure.</description><subject>550200 - Biochemistry</subject><subject>Agriculture</subject><subject>ANIMAL CELLS</subject><subject>ANIMALS</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Bioinformatics</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>CLONING</subject><subject>CONNECTIVE TISSUE CELLS</subject><subject>DNA-CLONING</subject><subject>ENZYME INHIBITORS</subject><subject>ENZYMES</subject><subject>FIBROBLASTS</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>FUNGI</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>HYDROLASES</subject><subject>Life Sciences</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>Methods. Procedures. Technologies</subject><subject>MICROORGANISMS</subject><subject>Molecular cloning</subject><subject>PEPTIDE HYDROLASES</subject><subject>PLANTS</subject><subject>PRIMATES</subject><subject>research-paper</subject><subject>SACCHAROMYCES</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>SOMATIC CELLS</subject><subject>VERTEBRATES</subject><subject>YEASTS</subject><issn>0733-222X</issn><issn>1087-0156</issn><issn>1546-1696</issn><issn>2331-3684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNpt0E1LxDAQBuAgCq4fJ_9AEG9aTdM0aY7r4hcoHlTQU0nT6W6kmyyZVNh_b6XiydMc5pkX5iXkJGeXOSuqK98kJiuVlbrcIbO8FDLLpZa7ZMZUUWSc8_d9coD4yZhQkosZ6Z9CD3boTaSLPnjnl9T4lr5sfVoBOqSho9cu9GHprOn7LZ3b5L6A3g9r4-mrQxyAPviVa1wK8Uc_QRph2MSQwHmDgNR5-gEG0xHZ60yPcPw7D8nb7c3r4j57fL57WMwfM1tInjJhqg6qxoA2bVnopmHAlLaiagshBStkVxkueVVJMK1WWhve5kxAK6DLhdDFITmdcgMmV6N1CezKBu_BplrlUkilRnQ-IRsDYoSu3kS3NnFb56z-abP-bbMe2xz12aQ3Bsciumi8dfh3ogTXUsmRXUwMx41fQqw_wxD9-Oy_qd_kxoTS</recordid><startdate>19870601</startdate><enddate>19870601</enddate><creator>Kaczorek, Michel</creator><creator>Honoré, Nadine</creator><creator>Ribes, Véronique</creator><creator>Dehoux, Pierre</creator><creator>Cornet, Philippe</creator><creator>Cartwright, Terence</creator><creator>Streeck, Rolf E.</creator><general>Nature Publishing Group US</general><general>Nature Publications</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>19870601</creationdate><title>Molecular Cloning and Synthesis of Biologically Active Human Tissue Inhibitor of Metalloproteinases in Yeast</title><author>Kaczorek, Michel ; Honoré, Nadine ; Ribes, Véronique ; Dehoux, Pierre ; Cornet, Philippe ; Cartwright, Terence ; Streeck, Rolf E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-4a8fe8bae9ad539bb0e079c48d3464036f8a262886ead9799a2d104ed4ef14493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>550200 - Biochemistry</topic><topic>Agriculture</topic><topic>ANIMAL CELLS</topic><topic>ANIMALS</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Bioinformatics</topic><topic>Biological and medical sciences</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>CLONING</topic><topic>CONNECTIVE TISSUE CELLS</topic><topic>DNA-CLONING</topic><topic>ENZYME INHIBITORS</topic><topic>ENZYMES</topic><topic>FIBROBLASTS</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>FUNGI</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>HYDROLASES</topic><topic>Life Sciences</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>Methods. Procedures. Technologies</topic><topic>MICROORGANISMS</topic><topic>Molecular cloning</topic><topic>PEPTIDE HYDROLASES</topic><topic>PLANTS</topic><topic>PRIMATES</topic><topic>research-paper</topic><topic>SACCHAROMYCES</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>SOMATIC CELLS</topic><topic>VERTEBRATES</topic><topic>YEASTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kaczorek, Michel</creatorcontrib><creatorcontrib>Honoré, Nadine</creatorcontrib><creatorcontrib>Ribes, Véronique</creatorcontrib><creatorcontrib>Dehoux, Pierre</creatorcontrib><creatorcontrib>Cornet, Philippe</creatorcontrib><creatorcontrib>Cartwright, Terence</creatorcontrib><creatorcontrib>Streeck, Rolf E.</creatorcontrib><creatorcontrib>Institut Pasteur, Paris (France)</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Bio/Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kaczorek, Michel</au><au>Honoré, Nadine</au><au>Ribes, Véronique</au><au>Dehoux, Pierre</au><au>Cornet, Philippe</au><au>Cartwright, Terence</au><au>Streeck, Rolf E.</au><aucorp>Institut Pasteur, Paris (France)</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Cloning and Synthesis of Biologically Active Human Tissue Inhibitor of Metalloproteinases in Yeast</atitle><jtitle>Bio/Technology</jtitle><stitle>Nat Biotechnol</stitle><date>1987-06-01</date><risdate>1987</risdate><volume>5</volume><issue>6</issue><spage>595</spage><epage>598</epage><pages>595-598</pages><issn>0733-222X</issn><issn>1087-0156</issn><eissn>1546-1696</eissn><eissn>2331-3684</eissn><abstract>Tissue inhibitor of metalloproteinases (TIMP) is a widely distributed glycoprotein that stochiometrically inactivates metalloproteinases involved in connective tissue catabolism. Here we report the cDNA cloning of TIMP from human fibroblastic MRC5 cells using a single 42–base oligonucleotide probe. Expression in
S. cerevisiae
of complete TIMP cDNA yielded insoluble protein aggregates. Biologically active TIMP was reconstituted from the yeast product by a denaturation/renaturation procedure.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><doi>10.1038/nbt0687-595</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0733-222X |
| ispartof | Bio/Technology, 1987-06, Vol.5 (6), p.595-598 |
| issn | 0733-222X 1087-0156 1546-1696 2331-3684 |
| language | eng |
| recordid | cdi_crossref_primary_10_1038_nbt0687_595 |
| source | Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | 550200 - Biochemistry Agriculture ANIMAL CELLS ANIMALS BASIC BIOLOGICAL SCIENCES Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology CLONING CONNECTIVE TISSUE CELLS DNA-CLONING ENZYME INHIBITORS ENZYMES FIBROBLASTS Fundamental and applied biological sciences. Psychology FUNGI Genetic engineering Genetic technics HYDROLASES Life Sciences MAMMALS MAN Methods. Procedures. Technologies MICROORGANISMS Molecular cloning PEPTIDE HYDROLASES PLANTS PRIMATES research-paper SACCHAROMYCES SACCHAROMYCES CEREVISIAE SOMATIC CELLS VERTEBRATES YEASTS |
| title | Molecular Cloning and Synthesis of Biologically Active Human Tissue Inhibitor of Metalloproteinases in Yeast |
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