Expression of a barley ribosome-inactivating protein leads to increased fungal protection in transgenic tobacco plants
We have obtained transgenic tobacco plants that express a barley ribosome inactivating protein (RIP) under control of the wound-inducible promoter of the potato wun1 gene. R 0 transformants selected on kanamycin were tested for growth in soil inoculated with the soil-borne phyto-pathogen Rhizoctonia...
Gespeichert in:
| Veröffentlicht in: | Bio/Technology 1992-03, Vol.10 (3), p.305-308 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 308 |
|---|---|
| container_issue | 3 |
| container_start_page | 305 |
| container_title | Bio/Technology |
| container_volume | 10 |
| creator | Logemann, J. (Max-Planck-Institut fur Zuchtungsforschung, Cologne, Germany) Jach, G Tommerup, H Mundy, J Schell, J |
| description | We have obtained transgenic tobacco plants that express a barley ribosome inactivating protein (RIP) under control of the wound-inducible promoter of the potato
wun1
gene. R
0
transformants selected on kanamycin were tested for growth in soil inoculated with the soil-borne phyto-pathogen
Rhizoctonia solani
. Three R
0
plants, which grew well compared to controls, and which were shown to carry the transgene by Southern blotting, were selfed and the R
1
progeny tested for protection against fungal inoculation. Southern and 2-D Western blotting showed that R
1
plants exhibiting increased protection had inherited the transgene and expressed the barley RIP upon wounding. |
| doi_str_mv | 10.1038/nbt0392-305 |
| format | Article |
| fullrecord | <record><control><sourceid>fao_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1038_nbt0392_305</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>US9190543</sourcerecordid><originalsourceid>FETCH-LOGICAL-c391t-50d3c9efdab451a4c9160285e27afa137209a47cd16d8f4da7afaf204d70f9eb3</originalsourceid><addsrcrecordid>eNp10MtLAzEQBvAgCtbqyZun3HU1r30dpdQHFDxowVuYzSZLyjYpSVr0vzdle_U0MPzmY_gQuqXkkRLePLkuEd6ygpPyDM0Y57TgVSPO0YzUnBeMse9LdBXjhhBRV0zM0GH5sws6Rusd9gYD7iCM-hcH2_not7qwDlSyB0jWDXgXfNLW4VFDH3Hy2DoVNETdY7N3A4yTyAc5LrsUwMVBO6sy7kApj3cjuBSv0YWBMeqb05yj9cvya_FWrD5e3xfPq0LxlqaiJD1XrTY9dKKkIFRLK8KaUrMaDFBeM9KCqFVPq74xoofj2jAi-pqYVnd8ju6nXBV8jEEbuQt2C-FXUiKPlclTZTJXlvXDpGNWbtBBbvw-uPzgP_xu4ga8hCHYKNefLW1JKTj_Azhleag</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Expression of a barley ribosome-inactivating protein leads to increased fungal protection in transgenic tobacco plants</title><source>Nature Journals Online</source><source>SpringerLink Journals - AutoHoldings</source><creator>Logemann, J. (Max-Planck-Institut fur Zuchtungsforschung, Cologne, Germany) ; Jach, G ; Tommerup, H ; Mundy, J ; Schell, J</creator><creatorcontrib>Logemann, J. (Max-Planck-Institut fur Zuchtungsforschung, Cologne, Germany) ; Jach, G ; Tommerup, H ; Mundy, J ; Schell, J</creatorcontrib><description>We have obtained transgenic tobacco plants that express a barley ribosome inactivating protein (RIP) under control of the wound-inducible promoter of the potato
wun1
gene. R
0
transformants selected on kanamycin were tested for growth in soil inoculated with the soil-borne phyto-pathogen
Rhizoctonia solani
. Three R
0
plants, which grew well compared to controls, and which were shown to carry the transgene by Southern blotting, were selfed and the R
1
progeny tested for protection against fungal inoculation. Southern and 2-D Western blotting showed that R
1
plants exhibiting increased protection had inherited the transgene and expressed the barley RIP upon wounding.</description><identifier>ISSN: 0733-222X</identifier><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 2331-3684</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt0392-305</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>ABIOTIC INJURIES ; Agriculture ; Bioinformatics ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; DISEASE RESISTANCE ; ENFERMEDADES FUNGOSAS ; FUNGAL DISEASES ; GENE ; GENE TRANSFER ; GENES ; GENETIC REGULATION ; GENETIC TRANSFORMATION ; GENETICA ; GENETICS ; GENETIQUE ; HORDEUM VULGARE ; INHIBITORS ; Life Sciences ; MALADIE FONGIQUE ; NICOTIANA TABACUM ; PLANT PROTEINS ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; PROMOTERS ; PROTEINAS VEGETALES ; PROTEINE VEGETALE ; RESISTANCE AUX MALADIES ; RESISTENCIA A LA ENFERMEDAD ; RHIZOCTONIA ; RHIZOCTONIA SOLANI ; RIBOSOMAS ; RIBOSOME ; RIBOSOMES ; SOLANUM TUBEROSUM ; STRUCTURAL GENES ; TRANSFERENCIA DE GENES ; TRANSFERT DE GENE ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; TRANSGENIC PLANTS ; TRANSLATION ; wun1 gene</subject><ispartof>Bio/Technology, 1992-03, Vol.10 (3), p.305-308</ispartof><rights>Nature Publishing Company 1992</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c391t-50d3c9efdab451a4c9160285e27afa137209a47cd16d8f4da7afaf204d70f9eb3</citedby><cites>FETCH-LOGICAL-c391t-50d3c9efdab451a4c9160285e27afa137209a47cd16d8f4da7afaf204d70f9eb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nbt0392-305$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nbt0392-305$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Logemann, J. (Max-Planck-Institut fur Zuchtungsforschung, Cologne, Germany)</creatorcontrib><creatorcontrib>Jach, G</creatorcontrib><creatorcontrib>Tommerup, H</creatorcontrib><creatorcontrib>Mundy, J</creatorcontrib><creatorcontrib>Schell, J</creatorcontrib><title>Expression of a barley ribosome-inactivating protein leads to increased fungal protection in transgenic tobacco plants</title><title>Bio/Technology</title><addtitle>Nat Biotechnol</addtitle><description>We have obtained transgenic tobacco plants that express a barley ribosome inactivating protein (RIP) under control of the wound-inducible promoter of the potato
wun1
gene. R
0
transformants selected on kanamycin were tested for growth in soil inoculated with the soil-borne phyto-pathogen
Rhizoctonia solani
. Three R
0
plants, which grew well compared to controls, and which were shown to carry the transgene by Southern blotting, were selfed and the R
1
progeny tested for protection against fungal inoculation. Southern and 2-D Western blotting showed that R
1
plants exhibiting increased protection had inherited the transgene and expressed the barley RIP upon wounding.</description><subject>ABIOTIC INJURIES</subject><subject>Agriculture</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>DISEASE RESISTANCE</subject><subject>ENFERMEDADES FUNGOSAS</subject><subject>FUNGAL DISEASES</subject><subject>GENE</subject><subject>GENE TRANSFER</subject><subject>GENES</subject><subject>GENETIC REGULATION</subject><subject>GENETIC TRANSFORMATION</subject><subject>GENETICA</subject><subject>GENETICS</subject><subject>GENETIQUE</subject><subject>HORDEUM VULGARE</subject><subject>INHIBITORS</subject><subject>Life Sciences</subject><subject>MALADIE FONGIQUE</subject><subject>NICOTIANA TABACUM</subject><subject>PLANT PROTEINS</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>PROMOTERS</subject><subject>PROTEINAS VEGETALES</subject><subject>PROTEINE VEGETALE</subject><subject>RESISTANCE AUX MALADIES</subject><subject>RESISTENCIA A LA ENFERMEDAD</subject><subject>RHIZOCTONIA</subject><subject>RHIZOCTONIA SOLANI</subject><subject>RIBOSOMAS</subject><subject>RIBOSOME</subject><subject>RIBOSOMES</subject><subject>SOLANUM TUBEROSUM</subject><subject>STRUCTURAL GENES</subject><subject>TRANSFERENCIA DE GENES</subject><subject>TRANSFERT DE GENE</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>TRANSGENIC PLANTS</subject><subject>TRANSLATION</subject><subject>wun1 gene</subject><issn>0733-222X</issn><issn>1087-0156</issn><issn>2331-3684</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><recordid>eNp10MtLAzEQBvAgCtbqyZun3HU1r30dpdQHFDxowVuYzSZLyjYpSVr0vzdle_U0MPzmY_gQuqXkkRLePLkuEd6ygpPyDM0Y57TgVSPO0YzUnBeMse9LdBXjhhBRV0zM0GH5sws6Rusd9gYD7iCM-hcH2_not7qwDlSyB0jWDXgXfNLW4VFDH3Hy2DoVNETdY7N3A4yTyAc5LrsUwMVBO6sy7kApj3cjuBSv0YWBMeqb05yj9cvya_FWrD5e3xfPq0LxlqaiJD1XrTY9dKKkIFRLK8KaUrMaDFBeM9KCqFVPq74xoofj2jAi-pqYVnd8ju6nXBV8jEEbuQt2C-FXUiKPlclTZTJXlvXDpGNWbtBBbvw-uPzgP_xu4ga8hCHYKNefLW1JKTj_Azhleag</recordid><startdate>19920301</startdate><enddate>19920301</enddate><creator>Logemann, J. (Max-Planck-Institut fur Zuchtungsforschung, Cologne, Germany)</creator><creator>Jach, G</creator><creator>Tommerup, H</creator><creator>Mundy, J</creator><creator>Schell, J</creator><general>Nature Publishing Group US</general><scope>FBQ</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19920301</creationdate><title>Expression of a barley ribosome-inactivating protein leads to increased fungal protection in transgenic tobacco plants</title><author>Logemann, J. (Max-Planck-Institut fur Zuchtungsforschung, Cologne, Germany) ; Jach, G ; Tommerup, H ; Mundy, J ; Schell, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c391t-50d3c9efdab451a4c9160285e27afa137209a47cd16d8f4da7afaf204d70f9eb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ABIOTIC INJURIES</topic><topic>Agriculture</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>DISEASE RESISTANCE</topic><topic>ENFERMEDADES FUNGOSAS</topic><topic>FUNGAL DISEASES</topic><topic>GENE</topic><topic>GENE TRANSFER</topic><topic>GENES</topic><topic>GENETIC REGULATION</topic><topic>GENETIC TRANSFORMATION</topic><topic>GENETICA</topic><topic>GENETICS</topic><topic>GENETIQUE</topic><topic>HORDEUM VULGARE</topic><topic>INHIBITORS</topic><topic>Life Sciences</topic><topic>MALADIE FONGIQUE</topic><topic>NICOTIANA TABACUM</topic><topic>PLANT PROTEINS</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>PROMOTERS</topic><topic>PROTEINAS VEGETALES</topic><topic>PROTEINE VEGETALE</topic><topic>RESISTANCE AUX MALADIES</topic><topic>RESISTENCIA A LA ENFERMEDAD</topic><topic>RHIZOCTONIA</topic><topic>RHIZOCTONIA SOLANI</topic><topic>RIBOSOMAS</topic><topic>RIBOSOME</topic><topic>RIBOSOMES</topic><topic>SOLANUM TUBEROSUM</topic><topic>STRUCTURAL GENES</topic><topic>TRANSFERENCIA DE GENES</topic><topic>TRANSFERT DE GENE</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><topic>TRANSGENIC PLANTS</topic><topic>TRANSLATION</topic><topic>wun1 gene</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Logemann, J. (Max-Planck-Institut fur Zuchtungsforschung, Cologne, Germany)</creatorcontrib><creatorcontrib>Jach, G</creatorcontrib><creatorcontrib>Tommerup, H</creatorcontrib><creatorcontrib>Mundy, J</creatorcontrib><creatorcontrib>Schell, J</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><jtitle>Bio/Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Logemann, J. (Max-Planck-Institut fur Zuchtungsforschung, Cologne, Germany)</au><au>Jach, G</au><au>Tommerup, H</au><au>Mundy, J</au><au>Schell, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a barley ribosome-inactivating protein leads to increased fungal protection in transgenic tobacco plants</atitle><jtitle>Bio/Technology</jtitle><stitle>Nat Biotechnol</stitle><date>1992-03-01</date><risdate>1992</risdate><volume>10</volume><issue>3</issue><spage>305</spage><epage>308</epage><pages>305-308</pages><issn>0733-222X</issn><issn>1087-0156</issn><eissn>2331-3684</eissn><eissn>1546-1696</eissn><abstract>We have obtained transgenic tobacco plants that express a barley ribosome inactivating protein (RIP) under control of the wound-inducible promoter of the potato
wun1
gene. R
0
transformants selected on kanamycin were tested for growth in soil inoculated with the soil-borne phyto-pathogen
Rhizoctonia solani
. Three R
0
plants, which grew well compared to controls, and which were shown to carry the transgene by Southern blotting, were selfed and the R
1
progeny tested for protection against fungal inoculation. Southern and 2-D Western blotting showed that R
1
plants exhibiting increased protection had inherited the transgene and expressed the barley RIP upon wounding.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><doi>10.1038/nbt0392-305</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0733-222X |
| ispartof | Bio/Technology, 1992-03, Vol.10 (3), p.305-308 |
| issn | 0733-222X 1087-0156 2331-3684 1546-1696 |
| language | eng |
| recordid | cdi_crossref_primary_10_1038_nbt0392_305 |
| source | Nature Journals Online; SpringerLink Journals - AutoHoldings |
| subjects | ABIOTIC INJURIES Agriculture Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology DISEASE RESISTANCE ENFERMEDADES FUNGOSAS FUNGAL DISEASES GENE GENE TRANSFER GENES GENETIC REGULATION GENETIC TRANSFORMATION GENETICA GENETICS GENETIQUE HORDEUM VULGARE INHIBITORS Life Sciences MALADIE FONGIQUE NICOTIANA TABACUM PLANT PROTEINS PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE PROMOTERS PROTEINAS VEGETALES PROTEINE VEGETALE RESISTANCE AUX MALADIES RESISTENCIA A LA ENFERMEDAD RHIZOCTONIA RHIZOCTONIA SOLANI RIBOSOMAS RIBOSOME RIBOSOMES SOLANUM TUBEROSUM STRUCTURAL GENES TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE TRANSGENIC PLANTS TRANSLATION wun1 gene |
| title | Expression of a barley ribosome-inactivating protein leads to increased fungal protection in transgenic tobacco plants |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T21%3A42%3A46IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-fao_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20a%20barley%20ribosome-inactivating%20protein%20leads%20to%20increased%20fungal%20protection%20in%20transgenic%20tobacco%20plants&rft.jtitle=Bio/Technology&rft.au=Logemann,%20J.%20(Max-Planck-Institut%20fur%20Zuchtungsforschung,%20Cologne,%20Germany)&rft.date=1992-03-01&rft.volume=10&rft.issue=3&rft.spage=305&rft.epage=308&rft.pages=305-308&rft.issn=0733-222X&rft.eissn=2331-3684&rft_id=info:doi/10.1038/nbt0392-305&rft_dat=%3Cfao_cross%3EUS9190543%3C/fao_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |