Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction
As a first example of the feasibility of producing industrial bulk enzymes in plants, we have expressed Bacillus licheniformis α-amylase in transgenic tobacco, and applied the seeds directly in starch liquification. The enzyme was properly secreted into the intercellular space, and maximum expressio...
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| Veröffentlicht in: | Bio/Technology 1992-03, Vol.10 (3), p.292-296 |
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| creator | Pen, J. (Research and Development, Delft, The Netherlands) Molendijk, L Quax, W.J Sijmons, P.C Ooyen, A.J.J. van Elzen, P.J.M. van den Rietveld, K Hoekema, A |
| description | As a first example of the feasibility of producing industrial bulk enzymes in plants, we have expressed
Bacillus licheniformis
α-amylase in transgenic tobacco, and applied the seeds directly in starch liquification. The enzyme was properly secreted into the intercellular space, and maximum expression levels of about 0.3% of total soluble protein were obtained. No apparent effect of the presence of the enzyme on plant phenotype was observed. The molecular weight of the enzyme produced in tobacco was around 64 kD. The difference, compared to 55.2 kD for the bacterial enzyme, was found to result from complex-type carbohydrate chains attached to the protein. Application studies on the liquefaction of starch were done with transgenic seeds containing the re-combinant α-amylase. The resulting hydrolysis products were virtually identical with those obtained from degradation with α-amylase from
Bacillus licheniformis
. |
| doi_str_mv | 10.1038/nbt0392-292 |
| format | Article |
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Bacillus licheniformis
α-amylase in transgenic tobacco, and applied the seeds directly in starch liquification. The enzyme was properly secreted into the intercellular space, and maximum expression levels of about 0.3% of total soluble protein were obtained. No apparent effect of the presence of the enzyme on plant phenotype was observed. The molecular weight of the enzyme produced in tobacco was around 64 kD. The difference, compared to 55.2 kD for the bacterial enzyme, was found to result from complex-type carbohydrate chains attached to the protein. Application studies on the liquefaction of starch were done with transgenic seeds containing the re-combinant α-amylase. The resulting hydrolysis products were virtually identical with those obtained from degradation with α-amylase from
Bacillus licheniformis
.</description><identifier>ISSN: 0733-222X</identifier><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 2331-3684</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/nbt0392-292</identifier><identifier>PMID: 1368101</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>ACTIVIDAD ENZIMATICA ; ACTIVITE ENZYMATIQUE ; Agriculture ; ALFA AMILASA ; ALMIDON ; ALPHA AMYLASE ; alpha-Amylases - metabolism ; AMIDON ; AMIDON DE POMME DE TERRE ; Bacillus - enzymology ; BACILLUS LICHENIFORMIS ; Bacterial Proteins - metabolism ; Bioinformatics ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Biomedicine ; Biotechnology ; ENZYMIC ACTIVITY ; EXPRESION GENICA ; EXPRESSION DES GENES ; Extracellular Space - metabolism ; FECULA DE LA PAPA ; FOOD INDUSTRY ; GENE ; GENE EXPRESSION ; GENES ; GENETIC TRANSFORMATION ; Genetic Vectors - genetics ; Glycosylation ; GRAINE ; HIDROLISIS ; HYDROLYSE ; HYDROLYSIS ; INDUSTRIA ALIMENTARIA ; INDUSTRIA ALMIDONERA ; INDUSTRIAL APPLICATIONS ; INDUSTRIE ALIMENTAIRE ; INDUSTRIE DE L'AMIDON ; Life Sciences ; MAIS ; MAIZ ; MAIZE ; Nicotiana - enzymology ; NICOTIANA TABACUM ; Phenotype ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants, Genetically Modified - enzymology ; Plants, Toxic ; POTATO STARCH ; SEEDS ; SEMILLA ; STARCH ; Starch - metabolism ; STARCH INDUSTRY ; STRUCTURAL GENES ; TRANSFORMACION GENETICA ; TRANSFORMATION GENETIQUE ; Transformation, Genetic - genetics ; TRANSGENIC PLANTS</subject><ispartof>Bio/Technology, 1992-03, Vol.10 (3), p.292-296</ispartof><rights>Nature Publishing Company 1992</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-261e411dec8e1279f109ef0eec7a1dfbac67765dd3fbedc26ea1e2c66b3498833</citedby><cites>FETCH-LOGICAL-c472t-261e411dec8e1279f109ef0eec7a1dfbac67765dd3fbedc26ea1e2c66b3498833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nbt0392-292$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nbt0392-292$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,2725,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1368101$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pen, J. (Research and Development, Delft, The Netherlands)</creatorcontrib><creatorcontrib>Molendijk, L</creatorcontrib><creatorcontrib>Quax, W.J</creatorcontrib><creatorcontrib>Sijmons, P.C</creatorcontrib><creatorcontrib>Ooyen, A.J.J. van</creatorcontrib><creatorcontrib>Elzen, P.J.M. van den</creatorcontrib><creatorcontrib>Rietveld, K</creatorcontrib><creatorcontrib>Hoekema, A</creatorcontrib><title>Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction</title><title>Bio/Technology</title><addtitle>Nat Biotechnol</addtitle><addtitle>Biotechnology (N Y)</addtitle><description>As a first example of the feasibility of producing industrial bulk enzymes in plants, we have expressed
Bacillus licheniformis
α-amylase in transgenic tobacco, and applied the seeds directly in starch liquification. The enzyme was properly secreted into the intercellular space, and maximum expression levels of about 0.3% of total soluble protein were obtained. No apparent effect of the presence of the enzyme on plant phenotype was observed. The molecular weight of the enzyme produced in tobacco was around 64 kD. The difference, compared to 55.2 kD for the bacterial enzyme, was found to result from complex-type carbohydrate chains attached to the protein. Application studies on the liquefaction of starch were done with transgenic seeds containing the re-combinant α-amylase. The resulting hydrolysis products were virtually identical with those obtained from degradation with α-amylase from
Bacillus licheniformis
.</description><subject>ACTIVIDAD ENZIMATICA</subject><subject>ACTIVITE ENZYMATIQUE</subject><subject>Agriculture</subject><subject>ALFA AMILASA</subject><subject>ALMIDON</subject><subject>ALPHA AMYLASE</subject><subject>alpha-Amylases - metabolism</subject><subject>AMIDON</subject><subject>AMIDON DE POMME DE TERRE</subject><subject>Bacillus - enzymology</subject><subject>BACILLUS LICHENIFORMIS</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bioinformatics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Biomedicine</subject><subject>Biotechnology</subject><subject>ENZYMIC ACTIVITY</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Extracellular Space - metabolism</subject><subject>FECULA DE LA PAPA</subject><subject>FOOD INDUSTRY</subject><subject>GENE</subject><subject>GENE EXPRESSION</subject><subject>GENES</subject><subject>GENETIC TRANSFORMATION</subject><subject>Genetic Vectors - genetics</subject><subject>Glycosylation</subject><subject>GRAINE</subject><subject>HIDROLISIS</subject><subject>HYDROLYSE</subject><subject>HYDROLYSIS</subject><subject>INDUSTRIA ALIMENTARIA</subject><subject>INDUSTRIA ALMIDONERA</subject><subject>INDUSTRIAL APPLICATIONS</subject><subject>INDUSTRIE ALIMENTAIRE</subject><subject>INDUSTRIE DE L'AMIDON</subject><subject>Life Sciences</subject><subject>MAIS</subject><subject>MAIZ</subject><subject>MAIZE</subject><subject>Nicotiana - enzymology</subject><subject>NICOTIANA TABACUM</subject><subject>Phenotype</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants, Genetically Modified - enzymology</subject><subject>Plants, Toxic</subject><subject>POTATO STARCH</subject><subject>SEEDS</subject><subject>SEMILLA</subject><subject>STARCH</subject><subject>Starch - metabolism</subject><subject>STARCH INDUSTRY</subject><subject>STRUCTURAL GENES</subject><subject>TRANSFORMACION GENETICA</subject><subject>TRANSFORMATION GENETIQUE</subject><subject>Transformation, Genetic - genetics</subject><subject>TRANSGENIC PLANTS</subject><issn>0733-222X</issn><issn>1087-0156</issn><issn>2331-3684</issn><issn>1546-1696</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtLxDAURoMoOo6u3AlCXGs1j9rHUsUXDCio4K7cpjdOpJPUpBX898bpoG5cJfCdnMv9QsgeZyecyeLU1j2TpUhEKdbIREjJE5kV6TqZsFzKRAjxskW2Q3hjLM0zkW6STR4BzviEuAfvmkH1xlnqNIV4-0B6Acq07RBoa9QcrdHOL0yg0HZzSGDx2UJAaiztXQ1KOQq2oaaPQNfFF7C0xTj04NU8St4H1LAcskM2NLQBd1fnlDxfXz1d3iaz-5u7y_NZotJc9InIOKacN6gK5CIvNWclaoaocuCNjlOzPM_OmkbqGhslMgSOQmVZLdOyKKSckqPRq7wLwaOuOm8W4D8rzqrv1qpVa1VsLdIHI90N9QKbX3asKebHYx5iYl_RV29u8DYu8I_ucMQt9IPHH92fj4rM_shocBW8ehOq58eSl-wsFfILYdqNEQ</recordid><startdate>19920301</startdate><enddate>19920301</enddate><creator>Pen, J. (Research and Development, Delft, The Netherlands)</creator><creator>Molendijk, L</creator><creator>Quax, W.J</creator><creator>Sijmons, P.C</creator><creator>Ooyen, A.J.J. van</creator><creator>Elzen, P.J.M. van den</creator><creator>Rietveld, K</creator><creator>Hoekema, A</creator><general>Nature Publishing Group US</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19920301</creationdate><title>Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction</title><author>Pen, J. (Research and Development, Delft, The Netherlands) ; Molendijk, L ; Quax, W.J ; Sijmons, P.C ; Ooyen, A.J.J. van ; Elzen, P.J.M. van den ; Rietveld, K ; Hoekema, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-261e411dec8e1279f109ef0eec7a1dfbac67765dd3fbedc26ea1e2c66b3498833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>ACTIVIDAD ENZIMATICA</topic><topic>ACTIVITE ENZYMATIQUE</topic><topic>Agriculture</topic><topic>ALFA AMILASA</topic><topic>ALMIDON</topic><topic>ALPHA AMYLASE</topic><topic>alpha-Amylases - metabolism</topic><topic>AMIDON</topic><topic>AMIDON DE POMME DE TERRE</topic><topic>Bacillus - enzymology</topic><topic>BACILLUS LICHENIFORMIS</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bioinformatics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Biomedicine</topic><topic>Biotechnology</topic><topic>ENZYMIC ACTIVITY</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Extracellular Space - metabolism</topic><topic>FECULA DE LA PAPA</topic><topic>FOOD INDUSTRY</topic><topic>GENE</topic><topic>GENE EXPRESSION</topic><topic>GENES</topic><topic>GENETIC TRANSFORMATION</topic><topic>Genetic Vectors - genetics</topic><topic>Glycosylation</topic><topic>GRAINE</topic><topic>HIDROLISIS</topic><topic>HYDROLYSE</topic><topic>HYDROLYSIS</topic><topic>INDUSTRIA ALIMENTARIA</topic><topic>INDUSTRIA ALMIDONERA</topic><topic>INDUSTRIAL APPLICATIONS</topic><topic>INDUSTRIE ALIMENTAIRE</topic><topic>INDUSTRIE DE L'AMIDON</topic><topic>Life Sciences</topic><topic>MAIS</topic><topic>MAIZ</topic><topic>MAIZE</topic><topic>Nicotiana - enzymology</topic><topic>NICOTIANA TABACUM</topic><topic>Phenotype</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants, Genetically Modified - enzymology</topic><topic>Plants, Toxic</topic><topic>POTATO STARCH</topic><topic>SEEDS</topic><topic>SEMILLA</topic><topic>STARCH</topic><topic>Starch - metabolism</topic><topic>STARCH INDUSTRY</topic><topic>STRUCTURAL GENES</topic><topic>TRANSFORMACION GENETICA</topic><topic>TRANSFORMATION GENETIQUE</topic><topic>Transformation, Genetic - genetics</topic><topic>TRANSGENIC PLANTS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pen, J. (Research and Development, Delft, The Netherlands)</creatorcontrib><creatorcontrib>Molendijk, L</creatorcontrib><creatorcontrib>Quax, W.J</creatorcontrib><creatorcontrib>Sijmons, P.C</creatorcontrib><creatorcontrib>Ooyen, A.J.J. van</creatorcontrib><creatorcontrib>Elzen, P.J.M. van den</creatorcontrib><creatorcontrib>Rietveld, K</creatorcontrib><creatorcontrib>Hoekema, A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Bio/Technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pen, J. (Research and Development, Delft, The Netherlands)</au><au>Molendijk, L</au><au>Quax, W.J</au><au>Sijmons, P.C</au><au>Ooyen, A.J.J. van</au><au>Elzen, P.J.M. van den</au><au>Rietveld, K</au><au>Hoekema, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction</atitle><jtitle>Bio/Technology</jtitle><stitle>Nat Biotechnol</stitle><addtitle>Biotechnology (N Y)</addtitle><date>1992-03-01</date><risdate>1992</risdate><volume>10</volume><issue>3</issue><spage>292</spage><epage>296</epage><pages>292-296</pages><issn>0733-222X</issn><issn>1087-0156</issn><eissn>2331-3684</eissn><eissn>1546-1696</eissn><abstract>As a first example of the feasibility of producing industrial bulk enzymes in plants, we have expressed
Bacillus licheniformis
α-amylase in transgenic tobacco, and applied the seeds directly in starch liquification. The enzyme was properly secreted into the intercellular space, and maximum expression levels of about 0.3% of total soluble protein were obtained. No apparent effect of the presence of the enzyme on plant phenotype was observed. The molecular weight of the enzyme produced in tobacco was around 64 kD. The difference, compared to 55.2 kD for the bacterial enzyme, was found to result from complex-type carbohydrate chains attached to the protein. Application studies on the liquefaction of starch were done with transgenic seeds containing the re-combinant α-amylase. The resulting hydrolysis products were virtually identical with those obtained from degradation with α-amylase from
Bacillus licheniformis
.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>1368101</pmid><doi>10.1038/nbt0392-292</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0733-222X |
| ispartof | Bio/Technology, 1992-03, Vol.10 (3), p.292-296 |
| issn | 0733-222X 1087-0156 2331-3684 1546-1696 |
| language | eng |
| recordid | cdi_crossref_primary_10_1038_nbt0392_292 |
| source | MEDLINE; Nature Journals Online; SpringerLink Journals - AutoHoldings |
| subjects | ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Agriculture ALFA AMILASA ALMIDON ALPHA AMYLASE alpha-Amylases - metabolism AMIDON AMIDON DE POMME DE TERRE Bacillus - enzymology BACILLUS LICHENIFORMIS Bacterial Proteins - metabolism Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology ENZYMIC ACTIVITY EXPRESION GENICA EXPRESSION DES GENES Extracellular Space - metabolism FECULA DE LA PAPA FOOD INDUSTRY GENE GENE EXPRESSION GENES GENETIC TRANSFORMATION Genetic Vectors - genetics Glycosylation GRAINE HIDROLISIS HYDROLYSE HYDROLYSIS INDUSTRIA ALIMENTARIA INDUSTRIA ALMIDONERA INDUSTRIAL APPLICATIONS INDUSTRIE ALIMENTAIRE INDUSTRIE DE L'AMIDON Life Sciences MAIS MAIZ MAIZE Nicotiana - enzymology NICOTIANA TABACUM Phenotype PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified - enzymology Plants, Toxic POTATO STARCH SEEDS SEMILLA STARCH Starch - metabolism STARCH INDUSTRY STRUCTURAL GENES TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE Transformation, Genetic - genetics TRANSGENIC PLANTS |
| title | Production of active Bacillus licheniformis alpha-amylase in tobacco and its application in starch liquefaction |
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