Autolytic Lactococcus lactis expressing a lactococcal bacteriophage lysin gene

We report the expression of a cloned lysin gene of bacteriophase ØvML3 in dairy starter strains of the species Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris . Lactococcal strains, which are sensitive to lysin, were unaffected by its expression during exponential growth but lysed aft...

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Veröffentlicht in:	Bio/Technology 1992-02, Vol.10 (2), p.196-199
Hauptverfasser:	Shearman, C.A. (AFRC Institute of Food Research, Norwich, U.K.), Jury, K, Gasson, M.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture autolysis BACTERIA BACTERIOFAGOS BACTERIOPHAGE BACTERIOPHAGES Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology CULTIVOS INICIADORES CULTURE STARTER CYTOLYSIS EXPRESION GENICA EXPRESSION DES GENES FERMENTACION FERMENTATION Fundamental and applied biological sciences. Psychology GENE GENE EXPRESSION GENE TRANSFER GENES Genetic engineering Genetic technics GENETIC TRANSFORMATION LACTOBACILLACEAE lactococcus lactis subsp. cremoris lactococcus lactis subsp. lactis LAIT LECHE Life Sciences Methods. Procedures. Technologies MILK Modification of gene expression level PROTEINAS PROTEINE PROTEINS research-paper STARTER CULTURES TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE VIRUS VIRUSES
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creator	Shearman, C.A. (AFRC Institute of Food Research, Norwich, U.K.) Jury, K Gasson, M.J
description	We report the expression of a cloned lysin gene of bacteriophase ØvML3 in dairy starter strains of the species Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris . Lactococcal strains, which are sensitive to lysin, were unaffected by its expression during exponential growth but lysed after reaching stationary phase in GM17 broth at 30°C. Lysis could be prevented by osmotic buffering of the medium with sucrose. When grown in milk to mimic dairy fermentations, L.lactis subsp. cremoris strains which expressed the lysin gene lost viability earlier than control strains even when stored at low temperature. This autolytic phenotype has the potential to be exploited in flavor acceleration strategies during cheese manufacture.
doi_str_mv	10.1038/nbt0292-196
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(AFRC Institute of Food Research, Norwich, U.K.)</creatorcontrib><creatorcontrib>Jury, K</creatorcontrib><creatorcontrib>Gasson, M.J</creatorcontrib><title>Autolytic Lactococcus lactis expressing a lactococcal bacteriophage lysin gene</title><title>Bio/Technology</title><addtitle>Nat Biotechnol</addtitle><description>We report the expression of a cloned lysin gene of bacteriophase ØvML3 in dairy starter strains of the species Lactococcus lactis subsp. lactis and L. lactis subsp. cremoris . Lactococcal strains, which are sensitive to lysin, were unaffected by its expression during exponential growth but lysed after reaching stationary phase in GM17 broth at 30°C. Lysis could be prevented by osmotic buffering of the medium with sucrose. When grown in milk to mimic dairy fermentations, L.lactis subsp. cremoris strains which expressed the lysin gene lost viability earlier than control strains even when stored at low temperature. 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source	Nature Journals Online; SpringerLink Journals - AutoHoldings
subjects	Agriculture autolysis BACTERIA BACTERIOFAGOS BACTERIOPHAGE BACTERIOPHAGES Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology CULTIVOS INICIADORES CULTURE STARTER CYTOLYSIS EXPRESION GENICA EXPRESSION DES GENES FERMENTACION FERMENTATION Fundamental and applied biological sciences. Psychology GENE GENE EXPRESSION GENE TRANSFER GENES Genetic engineering Genetic technics GENETIC TRANSFORMATION LACTOBACILLACEAE lactococcus lactis subsp. cremoris lactococcus lactis subsp. lactis LAIT LECHE Life Sciences Methods. Procedures. Technologies MILK Modification of gene expression level PROTEINAS PROTEINE PROTEINS research-paper STARTER CULTURES TRANSFERENCIA DE GENES TRANSFERT DE GENE TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE VIRUS VIRUSES
title	Autolytic Lactococcus lactis expressing a lactococcal bacteriophage lysin gene
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