Expression of a β-Lactamase Preproinsulin Fusion Protein in Escherichia Coli

The level of expression of a β-lactamase human preproinsulin fusion protein 1 in Escherichia coli was increased by plasmid and host manipulations. The initial expression level (0.01% of total protein) was increased two- to three-fold by replacing the p-lactamase promoter with two strong promoters: t...

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Veröffentlicht in:	Bio/Technology 1984-02, Vol.2 (2), p.165-168
Hauptverfasser:	Emerick, Anne W, Bertolani, Barbara L, Ben-Bassat, A, White, Thomas J, Konrad, Michael W
Format:	Artikel
Sprache:	eng
Schlagworte:	Agriculture Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Life Sciences
Online-Zugang:	Volltext
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