Tritium Labelling of Antibodies
A LARGE number of alkylating agents react with proteins while forming relatively stable compounds. Ethyl methane sulphonate (EMS) reacts in proteins mainly with non-ionized amino groups and ionized sulphydryl- and carboxyl-groups 1 . Below p H 8, the carboxyl groups constitute the majority of the al...
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| Veröffentlicht in: | Nature 1964-11, Vol.204 (4960), p.796-797 |
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| creator | ROSÉN, C.-G. EHRENBERG, L. AHNSTRÖM, G. |
| description | A LARGE number of alkylating agents react with proteins while forming relatively stable compounds. Ethyl methane sulphonate (EMS) reacts in proteins mainly with non-ionized amino groups and ionized sulphydryl- and carboxyl-groups
1
. Below
p
H 8, the carboxyl groups constitute the majority of the alkylated groups, and above this
p
H-level the amino groups are also alkylated. By varying the
p
H it is possible to vary the pattern of the groups which are alkylated
2
, and this can be utilized to attach tritium-labelled groups to proteins. In the work recorded here we used tritium-labelled EMS, which was synthesized from commercially available tritiated ethanol (ethanol-2-
T
, Radiochemical Centre, Amersham) and methanesulphonic anhydride. |
| doi_str_mv | 10.1038/204796a0 |
| format | Article |
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1
. Below
p
H 8, the carboxyl groups constitute the majority of the alkylated groups, and above this
p
H-level the amino groups are also alkylated. By varying the
p
H it is possible to vary the pattern of the groups which are alkylated
2
, and this can be utilized to attach tritium-labelled groups to proteins. In the work recorded here we used tritium-labelled EMS, which was synthesized from commercially available tritiated ethanol (ethanol-2-
T
, Radiochemical Centre, Amersham) and methanesulphonic anhydride.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/204796a0</identifier><identifier>PMID: 14235688</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>ACIDITY ; ALKYL RADICALS ; Alkylating Agents ; ANTIBODIES ; Autoradiography ; BIOLOGY AND MEDICINE ; CHEMICAL REACTIONS ; CONFIGURATION ; ELECTROPHORESIS ; Erythrocytes ; ETHYL RADICALS ; GLOBULINS ; HEATING ; Humanities and Social Sciences ; HYDROLYSIS ; IMMUNITY ; LABELLED COMPOUNDS ; letter ; MAN ; METHANE ; multidisciplinary ; ORGANIC SULFUR COMPOUNDS ; PROTEINS ; Radiometry ; Science ; Science (multidisciplinary) ; Serum Globulins ; STABILITY ; Sulfonic Acids ; TRITIUM ; VARIATIONS</subject><ispartof>Nature, 1964-11, Vol.204 (4960), p.796-797</ispartof><rights>Springer Nature Limited 1964</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-b65fff3c97540db3fc55bd6212aa9eeecc43129ff255a7bc77bd3ee7c71909cc3</citedby><cites>FETCH-LOGICAL-c342t-b65fff3c97540db3fc55bd6212aa9eeecc43129ff255a7bc77bd3ee7c71909cc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/204796a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/204796a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14235688$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/4683461$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>ROSÉN, C.-G.</creatorcontrib><creatorcontrib>EHRENBERG, L.</creatorcontrib><creatorcontrib>AHNSTRÖM, G.</creatorcontrib><creatorcontrib>Royal Univ. of Stockholm</creatorcontrib><title>Tritium Labelling of Antibodies</title><title>Nature</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>A LARGE number of alkylating agents react with proteins while forming relatively stable compounds. Ethyl methane sulphonate (EMS) reacts in proteins mainly with non-ionized amino groups and ionized sulphydryl- and carboxyl-groups
1
. Below
p
H 8, the carboxyl groups constitute the majority of the alkylated groups, and above this
p
H-level the amino groups are also alkylated. By varying the
p
H it is possible to vary the pattern of the groups which are alkylated
2
, and this can be utilized to attach tritium-labelled groups to proteins. In the work recorded here we used tritium-labelled EMS, which was synthesized from commercially available tritiated ethanol (ethanol-2-
T
, Radiochemical Centre, Amersham) and methanesulphonic anhydride.</description><subject>ACIDITY</subject><subject>ALKYL RADICALS</subject><subject>Alkylating Agents</subject><subject>ANTIBODIES</subject><subject>Autoradiography</subject><subject>BIOLOGY AND MEDICINE</subject><subject>CHEMICAL REACTIONS</subject><subject>CONFIGURATION</subject><subject>ELECTROPHORESIS</subject><subject>Erythrocytes</subject><subject>ETHYL RADICALS</subject><subject>GLOBULINS</subject><subject>HEATING</subject><subject>Humanities and Social Sciences</subject><subject>HYDROLYSIS</subject><subject>IMMUNITY</subject><subject>LABELLED COMPOUNDS</subject><subject>letter</subject><subject>MAN</subject><subject>METHANE</subject><subject>multidisciplinary</subject><subject>ORGANIC SULFUR COMPOUNDS</subject><subject>PROTEINS</subject><subject>Radiometry</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><subject>Serum Globulins</subject><subject>STABILITY</subject><subject>Sulfonic Acids</subject><subject>TRITIUM</subject><subject>VARIATIONS</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1964</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpl0EtLAzEUBeAgiq1V8BdocaWL0Zv3zLIUX1BwU9chuZPUlHamJNOF_74jU3Hh6i7ux4FzCLmm8EiBl08MhK6UhRMypkKrQqhSn5IxACsLKLkakYuc1wAgqRbnZEQF41KV5ZjcLlPs4n47XVjnN5vYrKZtmM6aLrq2jj5fkrNgN9lfHe-EfL48L-dvxeLj9X0-WxTIBesKp2QIgWOlpYDa8YBSuloxyqytvPeIglNWhcCktNqh1q7m3mvUtIIKkU_I3ZDb5i6ajLHz-IVt03jsTF-HC0V7dD8gTG3OyQezS3Fr07ehYH6GML9D9PRmoLu92_r6Dx6b9-BhALl_NSufzLrdp6bv-D_sAPMmY_Y</recordid><startdate>19641121</startdate><enddate>19641121</enddate><creator>ROSÉN, C.-G.</creator><creator>EHRENBERG, L.</creator><creator>AHNSTRÖM, G.</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>OTOTI</scope></search><sort><creationdate>19641121</creationdate><title>Tritium Labelling of Antibodies</title><author>ROSÉN, C.-G. ; EHRENBERG, L. ; AHNSTRÖM, G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-b65fff3c97540db3fc55bd6212aa9eeecc43129ff255a7bc77bd3ee7c71909cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1964</creationdate><topic>ACIDITY</topic><topic>ALKYL RADICALS</topic><topic>Alkylating Agents</topic><topic>ANTIBODIES</topic><topic>Autoradiography</topic><topic>BIOLOGY AND MEDICINE</topic><topic>CHEMICAL REACTIONS</topic><topic>CONFIGURATION</topic><topic>ELECTROPHORESIS</topic><topic>Erythrocytes</topic><topic>ETHYL RADICALS</topic><topic>GLOBULINS</topic><topic>HEATING</topic><topic>Humanities and Social Sciences</topic><topic>HYDROLYSIS</topic><topic>IMMUNITY</topic><topic>LABELLED COMPOUNDS</topic><topic>letter</topic><topic>MAN</topic><topic>METHANE</topic><topic>multidisciplinary</topic><topic>ORGANIC SULFUR COMPOUNDS</topic><topic>PROTEINS</topic><topic>Radiometry</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><topic>Serum Globulins</topic><topic>STABILITY</topic><topic>Sulfonic Acids</topic><topic>TRITIUM</topic><topic>VARIATIONS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ROSÉN, C.-G.</creatorcontrib><creatorcontrib>EHRENBERG, L.</creatorcontrib><creatorcontrib>AHNSTRÖM, G.</creatorcontrib><creatorcontrib>Royal Univ. of Stockholm</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>OSTI.GOV</collection><jtitle>Nature</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ROSÉN, C.-G.</au><au>EHRENBERG, L.</au><au>AHNSTRÖM, G.</au><aucorp>Royal Univ. of Stockholm</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tritium Labelling of Antibodies</atitle><jtitle>Nature</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1964-11-21</date><risdate>1964</risdate><volume>204</volume><issue>4960</issue><spage>796</spage><epage>797</epage><pages>796-797</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>A LARGE number of alkylating agents react with proteins while forming relatively stable compounds. Ethyl methane sulphonate (EMS) reacts in proteins mainly with non-ionized amino groups and ionized sulphydryl- and carboxyl-groups
1
. Below
p
H 8, the carboxyl groups constitute the majority of the alkylated groups, and above this
p
H-level the amino groups are also alkylated. By varying the
p
H it is possible to vary the pattern of the groups which are alkylated
2
, and this can be utilized to attach tritium-labelled groups to proteins. In the work recorded here we used tritium-labelled EMS, which was synthesized from commercially available tritiated ethanol (ethanol-2-
T
, Radiochemical Centre, Amersham) and methanesulphonic anhydride.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>14235688</pmid><doi>10.1038/204796a0</doi><tpages>2</tpages></addata></record> |
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| ispartof | Nature, 1964-11, Vol.204 (4960), p.796-797 |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | ACIDITY ALKYL RADICALS Alkylating Agents ANTIBODIES Autoradiography BIOLOGY AND MEDICINE CHEMICAL REACTIONS CONFIGURATION ELECTROPHORESIS Erythrocytes ETHYL RADICALS GLOBULINS HEATING Humanities and Social Sciences HYDROLYSIS IMMUNITY LABELLED COMPOUNDS letter MAN METHANE multidisciplinary ORGANIC SULFUR COMPOUNDS PROTEINS Radiometry Science Science (multidisciplinary) Serum Globulins STABILITY Sulfonic Acids TRITIUM VARIATIONS |
| title | Tritium Labelling of Antibodies |
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