Identification of Specific Protein Markers in Microdissected Hepatocellular Carcinoma
At present, the molecular mechanisms of hepatocellular carcinogenesis are not well-understood, and hepatocellular carcinoma (HCC) stays one of the most frequent and high-risk metastatic visceral neoplasms worldwide. For the identification of tumor-relevant proteins, we analyzed microdissected cells...
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| Veröffentlicht in: | Journal of proteome research 2007-01, Vol.6 (1), p.306-315 |
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| creator | Melle, Christian Ernst, Günther Scheibner, Olaf Kaufmann, Roland Schimmel, Bettina Bleul, Annett Settmacher, Utz Hommann, Merten Claussen, Uwe von Eggeling, Ferdinand |
| description | At present, the molecular mechanisms of hepatocellular carcinogenesis are not well-understood, and hepatocellular carcinoma (HCC) stays one of the most frequent and high-risk metastatic visceral neoplasms worldwide. For the identification of tumor-relevant proteins, we analyzed microdissected cells from nontumorous liver tissue (n = 28) and tissue derived from hepatic tumor center (n = 25), as well as tumor margin (n = 23). We unequivocally identified 53 proteins from hepatic tumor tissues by peptide fingerprint mapping and SELDI mass spectrometry that were separated using two-dimensional gel electrophoresis. Among a number of signals that were detected as significantly different in the protein profiling analysis, we identified for the first time ferritin light subunit (FLS) and adenylate kinase 3 alpha-like 1 (AK3), showing decreased expressions in hepatic tumor, as well as biliverdin reductase B (BVRB) that was upregulated in HCC. The use of ProteinChip technology in combination with tissue microdissection gives insight of the complex changes occurring at the protein level in hepatocellular cancer associated with tumor development and progression and resulted in three new potential diagnostically useful markers. Keywords: hepatocellular carcinoma • proteomics • biomarker • tissue microdissection • SELDI-TOF MS • tandem MS • two-dimensional gel electrophoresis • immunohistochemistry |
| doi_str_mv | 10.1021/pr060439b |
| format | Article |
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For the identification of tumor-relevant proteins, we analyzed microdissected cells from nontumorous liver tissue (n = 28) and tissue derived from hepatic tumor center (n = 25), as well as tumor margin (n = 23). We unequivocally identified 53 proteins from hepatic tumor tissues by peptide fingerprint mapping and SELDI mass spectrometry that were separated using two-dimensional gel electrophoresis. Among a number of signals that were detected as significantly different in the protein profiling analysis, we identified for the first time ferritin light subunit (FLS) and adenylate kinase 3 alpha-like 1 (AK3), showing decreased expressions in hepatic tumor, as well as biliverdin reductase B (BVRB) that was upregulated in HCC. The use of ProteinChip technology in combination with tissue microdissection gives insight of the complex changes occurring at the protein level in hepatocellular cancer associated with tumor development and progression and resulted in three new potential diagnostically useful markers. 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Proteome Res</addtitle><description>At present, the molecular mechanisms of hepatocellular carcinogenesis are not well-understood, and hepatocellular carcinoma (HCC) stays one of the most frequent and high-risk metastatic visceral neoplasms worldwide. For the identification of tumor-relevant proteins, we analyzed microdissected cells from nontumorous liver tissue (n = 28) and tissue derived from hepatic tumor center (n = 25), as well as tumor margin (n = 23). We unequivocally identified 53 proteins from hepatic tumor tissues by peptide fingerprint mapping and SELDI mass spectrometry that were separated using two-dimensional gel electrophoresis. Among a number of signals that were detected as significantly different in the protein profiling analysis, we identified for the first time ferritin light subunit (FLS) and adenylate kinase 3 alpha-like 1 (AK3), showing decreased expressions in hepatic tumor, as well as biliverdin reductase B (BVRB) that was upregulated in HCC. The use of ProteinChip technology in combination with tissue microdissection gives insight of the complex changes occurring at the protein level in hepatocellular cancer associated with tumor development and progression and resulted in three new potential diagnostically useful markers. 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Proteome Res</addtitle><date>2007-01</date><risdate>2007</risdate><volume>6</volume><issue>1</issue><spage>306</spage><epage>315</epage><pages>306-315</pages><issn>1535-3893</issn><eissn>1535-3907</eissn><abstract>At present, the molecular mechanisms of hepatocellular carcinogenesis are not well-understood, and hepatocellular carcinoma (HCC) stays one of the most frequent and high-risk metastatic visceral neoplasms worldwide. For the identification of tumor-relevant proteins, we analyzed microdissected cells from nontumorous liver tissue (n = 28) and tissue derived from hepatic tumor center (n = 25), as well as tumor margin (n = 23). We unequivocally identified 53 proteins from hepatic tumor tissues by peptide fingerprint mapping and SELDI mass spectrometry that were separated using two-dimensional gel electrophoresis. Among a number of signals that were detected as significantly different in the protein profiling analysis, we identified for the first time ferritin light subunit (FLS) and adenylate kinase 3 alpha-like 1 (AK3), showing decreased expressions in hepatic tumor, as well as biliverdin reductase B (BVRB) that was upregulated in HCC. The use of ProteinChip technology in combination with tissue microdissection gives insight of the complex changes occurring at the protein level in hepatocellular cancer associated with tumor development and progression and resulted in three new potential diagnostically useful markers. Keywords: hepatocellular carcinoma • proteomics • biomarker • tissue microdissection • SELDI-TOF MS • tandem MS • two-dimensional gel electrophoresis • immunohistochemistry</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>17203974</pmid><doi>10.1021/pr060439b</doi><tpages>10</tpages></addata></record> |
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| subjects | Biomarkers Carcinoma, Hepatocellular - metabolism Electrophoresis, Gel, Two-Dimensional Gene Expression Regulation, Neoplastic Humans Immunohistochemistry Lasers Liver - metabolism Liver Neoplasms - metabolism Neoplasm Metastasis Neoplasm Proteins - metabolism Prognosis Protein Array Analysis Proteomics - methods |
| title | Identification of Specific Protein Markers in Microdissected Hepatocellular Carcinoma |
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