Quantitative HPLC Analysis of the Level of Fecapentaenes and Their Precursors in Human Feces by a Chemical Conversion Method
A fast and reliable hplc method for the quantitative analysis of total fecapentaene-12 (FP-12 and its precursors) and total fecapentaene-14 (FP-14 and its precursors) in human feces is described. The analysis is based on the rapid chemical conversion of fecapentaenes and their precursors to more sta...
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| Veröffentlicht in: | Journal of natural products (Washington, D.C.) D.C.), 1990-01, Vol.53 (1), p.42-49 |
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| container_end_page | 49 |
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| container_issue | 1 |
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| container_title | Journal of natural products (Washington, D.C.) |
| container_volume | 53 |
| creator | Kivits, G. A. A de Boer, B. C. J Nugteren, D. H van der Steeg, M Vertegaal, L. B. J van der Gen, A |
| description | A fast and reliable hplc method for the quantitative analysis of total fecapentaene-12 (FP-12 and its precursors) and total fecapentaene-14 (FP-14 and its precursors) in human feces is described. The analysis is based on the rapid chemical conversion of fecapentaenes and their precursors to more stable methoxytetraenols and the use of synthetic, not naturally occurring, fecapentaene-13 (FP-13) as an internal standard. The synthesis and physical properties of this internal standard are described. The convenience and reproducibility of the method were illustrated by applying the procedure to stool samples obtained from twelve individuals on 3 consecutive days. Levels of total FP's were in the range of 0.1-25.4 micrograms for total FP-12 and 0.1-8.5 micrograms for total FP-14 per g of wet feces. Appreciable fluctuations were observed between levels in samples from the same individual on different days. Reproducibility and recovery were shown to be good. |
| doi_str_mv | 10.1021/np50067a004 |
| format | Article |
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A. A ; de Boer, B. C. J ; Nugteren, D. H ; van der Steeg, M ; Vertegaal, L. B. J ; van der Gen, A</creator><creatorcontrib>Kivits, G. A. A ; de Boer, B. C. J ; Nugteren, D. H ; van der Steeg, M ; Vertegaal, L. B. J ; van der Gen, A</creatorcontrib><description>A fast and reliable hplc method for the quantitative analysis of total fecapentaene-12 (FP-12 and its precursors) and total fecapentaene-14 (FP-14 and its precursors) in human feces is described. The analysis is based on the rapid chemical conversion of fecapentaenes and their precursors to more stable methoxytetraenols and the use of synthetic, not naturally occurring, fecapentaene-13 (FP-13) as an internal standard. The synthesis and physical properties of this internal standard are described. The convenience and reproducibility of the method were illustrated by applying the procedure to stool samples obtained from twelve individuals on 3 consecutive days. Levels of total FP's were in the range of 0.1-25.4 micrograms for total FP-12 and 0.1-8.5 micrograms for total FP-14 per g of wet feces. Appreciable fluctuations were observed between levels in samples from the same individual on different days. Reproducibility and recovery were shown to be good.</description><identifier>ISSN: 0163-3864</identifier><identifier>EISSN: 1520-6025</identifier><identifier>DOI: 10.1021/np50067a004</identifier><identifier>PMID: 2348203</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Adult ; Chromatography, High Pressure Liquid ; Feces - analysis ; Humans ; Male ; Middle Aged ; Mutagens - analysis ; Polyenes - analysis ; Spectrum Analysis</subject><ispartof>Journal of natural products (Washington, D.C.), 1990-01, Vol.53 (1), p.42-49</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a354t-73491e385ca2ead1756ff22f4aa3d44928c1a851b37fe62e9e7c56f985959b0e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/np50067a004$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/np50067a004$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2348203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kivits, G. A. A</creatorcontrib><creatorcontrib>de Boer, B. C. J</creatorcontrib><creatorcontrib>Nugteren, D. H</creatorcontrib><creatorcontrib>van der Steeg, M</creatorcontrib><creatorcontrib>Vertegaal, L. B. J</creatorcontrib><creatorcontrib>van der Gen, A</creatorcontrib><title>Quantitative HPLC Analysis of the Level of Fecapentaenes and Their Precursors in Human Feces by a Chemical Conversion Method</title><title>Journal of natural products (Washington, D.C.)</title><addtitle>J. Nat. Prod</addtitle><description>A fast and reliable hplc method for the quantitative analysis of total fecapentaene-12 (FP-12 and its precursors) and total fecapentaene-14 (FP-14 and its precursors) in human feces is described. The analysis is based on the rapid chemical conversion of fecapentaenes and their precursors to more stable methoxytetraenols and the use of synthetic, not naturally occurring, fecapentaene-13 (FP-13) as an internal standard. The synthesis and physical properties of this internal standard are described. The convenience and reproducibility of the method were illustrated by applying the procedure to stool samples obtained from twelve individuals on 3 consecutive days. Levels of total FP's were in the range of 0.1-25.4 micrograms for total FP-12 and 0.1-8.5 micrograms for total FP-14 per g of wet feces. Appreciable fluctuations were observed between levels in samples from the same individual on different days. Reproducibility and recovery were shown to be good.</description><subject>Adult</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Feces - analysis</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mutagens - analysis</subject><subject>Polyenes - analysis</subject><subject>Spectrum Analysis</subject><issn>0163-3864</issn><issn>1520-6025</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0E1r20AQBuCltKRumlPPhb31EJTO7mr1cTRqbQcU4lAngV6WsTTC69orsyuZGPLjK-MQcshpGN6HGXgZ-ybgSoAUP91OAyQpAsQf2EhoCVECUn9kIxCJilSWxJ_ZlxDWAKAg12fsTKo4k6BG7PmuR9fZDju7Jz6blwUfO9wcgg28bXi3Il7SnjbHZUIV7sh1SI4CR1fzxYqs53NPVe9D6wO3js_6LbqjHczywJEXK9raCje8aN2efLCt4zfUrdr6K_vU4CbQxcs8Z_eT34tiFpW30-tiXEaodNxFqYpzQSrTFUrCWqQ6aRopmxhR1XGcy6wSmGmxVGlDiaSc0mogeaZznS-B1Dm7PN2tfBuCp8bsvN2iPxgB5liheVPhoL-f9K5fbql-tS-dDXl0ym3o6Ok1Rv_PJKlKtVnM_5hHyMtf6d8HMx38j5PHKph12_uh3_Du5_-RlofY</recordid><startdate>19900101</startdate><enddate>19900101</enddate><creator>Kivits, G. A. A</creator><creator>de Boer, B. C. J</creator><creator>Nugteren, D. H</creator><creator>van der Steeg, M</creator><creator>Vertegaal, L. B. J</creator><creator>van der Gen, A</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19900101</creationdate><title>Quantitative HPLC Analysis of the Level of Fecapentaenes and Their Precursors in Human Feces by a Chemical Conversion Method</title><author>Kivits, G. A. A ; de Boer, B. C. J ; Nugteren, D. H ; van der Steeg, M ; Vertegaal, L. B. J ; van der Gen, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a354t-73491e385ca2ead1756ff22f4aa3d44928c1a851b37fe62e9e7c56f985959b0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Adult</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Feces - analysis</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mutagens - analysis</topic><topic>Polyenes - analysis</topic><topic>Spectrum Analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kivits, G. A. A</creatorcontrib><creatorcontrib>de Boer, B. C. J</creatorcontrib><creatorcontrib>Nugteren, D. H</creatorcontrib><creatorcontrib>van der Steeg, M</creatorcontrib><creatorcontrib>Vertegaal, L. B. J</creatorcontrib><creatorcontrib>van der Gen, A</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of natural products (Washington, D.C.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kivits, G. A. A</au><au>de Boer, B. C. J</au><au>Nugteren, D. H</au><au>van der Steeg, M</au><au>Vertegaal, L. B. J</au><au>van der Gen, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative HPLC Analysis of the Level of Fecapentaenes and Their Precursors in Human Feces by a Chemical Conversion Method</atitle><jtitle>Journal of natural products (Washington, D.C.)</jtitle><addtitle>J. Nat. Prod</addtitle><date>1990-01-01</date><risdate>1990</risdate><volume>53</volume><issue>1</issue><spage>42</spage><epage>49</epage><pages>42-49</pages><issn>0163-3864</issn><eissn>1520-6025</eissn><abstract>A fast and reliable hplc method for the quantitative analysis of total fecapentaene-12 (FP-12 and its precursors) and total fecapentaene-14 (FP-14 and its precursors) in human feces is described. The analysis is based on the rapid chemical conversion of fecapentaenes and their precursors to more stable methoxytetraenols and the use of synthetic, not naturally occurring, fecapentaene-13 (FP-13) as an internal standard. The synthesis and physical properties of this internal standard are described. The convenience and reproducibility of the method were illustrated by applying the procedure to stool samples obtained from twelve individuals on 3 consecutive days. Levels of total FP's were in the range of 0.1-25.4 micrograms for total FP-12 and 0.1-8.5 micrograms for total FP-14 per g of wet feces. Appreciable fluctuations were observed between levels in samples from the same individual on different days. Reproducibility and recovery were shown to be good.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>2348203</pmid><doi>10.1021/np50067a004</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0163-3864 |
| ispartof | Journal of natural products (Washington, D.C.), 1990-01, Vol.53 (1), p.42-49 |
| issn | 0163-3864 1520-6025 |
| language | eng |
| recordid | cdi_crossref_primary_10_1021_np50067a004 |
| source | ACS Publications; MEDLINE |
| subjects | Adult Chromatography, High Pressure Liquid Feces - analysis Humans Male Middle Aged Mutagens - analysis Polyenes - analysis Spectrum Analysis |
| title | Quantitative HPLC Analysis of the Level of Fecapentaenes and Their Precursors in Human Feces by a Chemical Conversion Method |
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