Effects of Cell Culture Media on the Dynamic Formation of Protein−Nanoparticle Complexes and Influence on the Cellular Response
The development of appropriate in vitro protocols to assess the potential toxicity of the ever expanding range of nanoparticles represents a challenging issue, because of the rapid changes of their intrinsic physicochemical properties (size, shape, reactivity, surface area, etc.) upon dispersion in...
Gespeichert in:
| Veröffentlicht in: | ACS nano 2010-12, Vol.4 (12), p.7481-7491 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7491 |
|---|---|
| container_issue | 12 |
| container_start_page | 7481 |
| container_title | ACS nano |
| container_volume | 4 |
| creator | Maiorano, Gabriele Sabella, Stefania Sorce, Barbara Brunetti, Virgilio Malvindi, Maria Ada Cingolani, Roberto Pompa, Pier Paolo |
| description | The development of appropriate in vitro protocols to assess the potential toxicity of the ever expanding range of nanoparticles represents a challenging issue, because of the rapid changes of their intrinsic physicochemical properties (size, shape, reactivity, surface area, etc.) upon dispersion in biological fluids. Dynamic formation of protein coating around nanoparticles is a key molecular event, which may strongly impact the biological response in nanotoxicological tests. In this work, by using citrate-capped gold nanoparticles (AuNPs) of different sizes as a model, we show, by several spectroscopic techniques (dynamic light scattering, UV−visible, plasmon resonance light scattering), that proteins−NP interactions are differently mediated by two widely used cellular media (i.e., Dulbecco Modified Eagle’s medium (DMEM) and Roswell Park Memorial Institute medium (RPMI), supplemented with fetal bovine serum). We found that, while DMEM elicits the formation of a large time-dependent protein corona, RPMI shows different dynamics with reduced protein coating. Characterization of these nanobioentities was also performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and mass spectroscopy, revealing that the average composition of protein corona does not reflect the relative abundance of serum proteins. To evaluate the biological impact of such hybrid bionanostructures, several comparative viability assays onto two cell lines (HeLa and U937) were carried out in the two media, in the presence of 15 nm AuNPs. We observed that proteins/NP complexes formed in RPMI are more abundantly internalized in cells as compared to DMEM, overall exerting higher cytotoxic effects. These results show that, beyond an in-depth NPs characterization before cellular experiments, a detailed understanding of the effects elicited by cell culture media on NPs is crucial for standardized nanotoxicology tests. |
| doi_str_mv | 10.1021/nn101557e |
| format | Article |
| fullrecord | <record><control><sourceid>acs_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1021_nn101557e</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>f12023682</sourcerecordid><originalsourceid>FETCH-LOGICAL-a415t-7b7fd1895e07321c8e286b8822134347284740d73b5846b051ab74882ebee3a83</originalsourceid><addsrcrecordid>eNptkL9OwzAQhy0EoqUw8ALICwNDwXbs2B1RaKFS-SMEElvkJBeRyrEjO5HoyMbMI_IkpCrtxHSnu0-_O30InVJySQmjV9ZSQoWQsIeGdBLFY6Lit_1dL-gAHYWwJERIJeNDNGCUKKYoH6LPaVlC3gbsSpyAMTjpTNt5wPdQVBo7i9t3wDcrq-sqxzPna91W_bTHn7xrobI_X98P2rpG-7bKDeDE1Y2BDwhY2wLPbWk6sDlso9ZHOqM9fobQOBvgGB2U2gQ4-asj9DqbviR348Xj7Ty5Xow1p6Idy0yWBVUTAURGjOYKmIozpRijEY-4ZIpLTgoZZULxOCOC6kzyfg8ZQKRVNEIXm9zcuxA8lGnjq1r7VUpJutaY7jT27NmGbbqshmJHbr31wPkG0HlIl67ztn_9n6BfxjB6jQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Effects of Cell Culture Media on the Dynamic Formation of Protein−Nanoparticle Complexes and Influence on the Cellular Response</title><source>MEDLINE</source><source>American Chemical Society Journals</source><creator>Maiorano, Gabriele ; Sabella, Stefania ; Sorce, Barbara ; Brunetti, Virgilio ; Malvindi, Maria Ada ; Cingolani, Roberto ; Pompa, Pier Paolo</creator><creatorcontrib>Maiorano, Gabriele ; Sabella, Stefania ; Sorce, Barbara ; Brunetti, Virgilio ; Malvindi, Maria Ada ; Cingolani, Roberto ; Pompa, Pier Paolo</creatorcontrib><description>The development of appropriate in vitro protocols to assess the potential toxicity of the ever expanding range of nanoparticles represents a challenging issue, because of the rapid changes of their intrinsic physicochemical properties (size, shape, reactivity, surface area, etc.) upon dispersion in biological fluids. Dynamic formation of protein coating around nanoparticles is a key molecular event, which may strongly impact the biological response in nanotoxicological tests. In this work, by using citrate-capped gold nanoparticles (AuNPs) of different sizes as a model, we show, by several spectroscopic techniques (dynamic light scattering, UV−visible, plasmon resonance light scattering), that proteins−NP interactions are differently mediated by two widely used cellular media (i.e., Dulbecco Modified Eagle’s medium (DMEM) and Roswell Park Memorial Institute medium (RPMI), supplemented with fetal bovine serum). We found that, while DMEM elicits the formation of a large time-dependent protein corona, RPMI shows different dynamics with reduced protein coating. Characterization of these nanobioentities was also performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and mass spectroscopy, revealing that the average composition of protein corona does not reflect the relative abundance of serum proteins. To evaluate the biological impact of such hybrid bionanostructures, several comparative viability assays onto two cell lines (HeLa and U937) were carried out in the two media, in the presence of 15 nm AuNPs. We observed that proteins/NP complexes formed in RPMI are more abundantly internalized in cells as compared to DMEM, overall exerting higher cytotoxic effects. These results show that, beyond an in-depth NPs characterization before cellular experiments, a detailed understanding of the effects elicited by cell culture media on NPs is crucial for standardized nanotoxicology tests.</description><identifier>ISSN: 1936-0851</identifier><identifier>EISSN: 1936-086X</identifier><identifier>DOI: 10.1021/nn101557e</identifier><identifier>PMID: 21082814</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Animals ; Cattle ; Cell Culture Techniques - methods ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Citrates - chemistry ; Culture Media - pharmacology ; Gold - chemistry ; Gold - metabolism ; Gold - toxicity ; Humans ; Metal Nanoparticles - chemistry ; Nanoparticles - chemistry ; Nanoparticles - toxicity ; Particle Size ; Protein Binding - drug effects ; Proteins - metabolism</subject><ispartof>ACS nano, 2010-12, Vol.4 (12), p.7481-7491</ispartof><rights>Copyright © 2010 American Chemical Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a415t-7b7fd1895e07321c8e286b8822134347284740d73b5846b051ab74882ebee3a83</citedby><cites>FETCH-LOGICAL-a415t-7b7fd1895e07321c8e286b8822134347284740d73b5846b051ab74882ebee3a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/nn101557e$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/nn101557e$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21082814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maiorano, Gabriele</creatorcontrib><creatorcontrib>Sabella, Stefania</creatorcontrib><creatorcontrib>Sorce, Barbara</creatorcontrib><creatorcontrib>Brunetti, Virgilio</creatorcontrib><creatorcontrib>Malvindi, Maria Ada</creatorcontrib><creatorcontrib>Cingolani, Roberto</creatorcontrib><creatorcontrib>Pompa, Pier Paolo</creatorcontrib><title>Effects of Cell Culture Media on the Dynamic Formation of Protein−Nanoparticle Complexes and Influence on the Cellular Response</title><title>ACS nano</title><addtitle>ACS Nano</addtitle><description>The development of appropriate in vitro protocols to assess the potential toxicity of the ever expanding range of nanoparticles represents a challenging issue, because of the rapid changes of their intrinsic physicochemical properties (size, shape, reactivity, surface area, etc.) upon dispersion in biological fluids. Dynamic formation of protein coating around nanoparticles is a key molecular event, which may strongly impact the biological response in nanotoxicological tests. In this work, by using citrate-capped gold nanoparticles (AuNPs) of different sizes as a model, we show, by several spectroscopic techniques (dynamic light scattering, UV−visible, plasmon resonance light scattering), that proteins−NP interactions are differently mediated by two widely used cellular media (i.e., Dulbecco Modified Eagle’s medium (DMEM) and Roswell Park Memorial Institute medium (RPMI), supplemented with fetal bovine serum). We found that, while DMEM elicits the formation of a large time-dependent protein corona, RPMI shows different dynamics with reduced protein coating. Characterization of these nanobioentities was also performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and mass spectroscopy, revealing that the average composition of protein corona does not reflect the relative abundance of serum proteins. To evaluate the biological impact of such hybrid bionanostructures, several comparative viability assays onto two cell lines (HeLa and U937) were carried out in the two media, in the presence of 15 nm AuNPs. We observed that proteins/NP complexes formed in RPMI are more abundantly internalized in cells as compared to DMEM, overall exerting higher cytotoxic effects. These results show that, beyond an in-depth NPs characterization before cellular experiments, a detailed understanding of the effects elicited by cell culture media on NPs is crucial for standardized nanotoxicology tests.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Citrates - chemistry</subject><subject>Culture Media - pharmacology</subject><subject>Gold - chemistry</subject><subject>Gold - metabolism</subject><subject>Gold - toxicity</subject><subject>Humans</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - toxicity</subject><subject>Particle Size</subject><subject>Protein Binding - drug effects</subject><subject>Proteins - metabolism</subject><issn>1936-0851</issn><issn>1936-086X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkL9OwzAQhy0EoqUw8ALICwNDwXbs2B1RaKFS-SMEElvkJBeRyrEjO5HoyMbMI_IkpCrtxHSnu0-_O30InVJySQmjV9ZSQoWQsIeGdBLFY6Lit_1dL-gAHYWwJERIJeNDNGCUKKYoH6LPaVlC3gbsSpyAMTjpTNt5wPdQVBo7i9t3wDcrq-sqxzPna91W_bTHn7xrobI_X98P2rpG-7bKDeDE1Y2BDwhY2wLPbWk6sDlso9ZHOqM9fobQOBvgGB2U2gQ4-asj9DqbviR348Xj7Ty5Xow1p6Idy0yWBVUTAURGjOYKmIozpRijEY-4ZIpLTgoZZULxOCOC6kzyfg8ZQKRVNEIXm9zcuxA8lGnjq1r7VUpJutaY7jT27NmGbbqshmJHbr31wPkG0HlIl67ztn_9n6BfxjB6jQ</recordid><startdate>20101228</startdate><enddate>20101228</enddate><creator>Maiorano, Gabriele</creator><creator>Sabella, Stefania</creator><creator>Sorce, Barbara</creator><creator>Brunetti, Virgilio</creator><creator>Malvindi, Maria Ada</creator><creator>Cingolani, Roberto</creator><creator>Pompa, Pier Paolo</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20101228</creationdate><title>Effects of Cell Culture Media on the Dynamic Formation of Protein−Nanoparticle Complexes and Influence on the Cellular Response</title><author>Maiorano, Gabriele ; Sabella, Stefania ; Sorce, Barbara ; Brunetti, Virgilio ; Malvindi, Maria Ada ; Cingolani, Roberto ; Pompa, Pier Paolo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a415t-7b7fd1895e07321c8e286b8822134347284740d73b5846b051ab74882ebee3a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Citrates - chemistry</topic><topic>Culture Media - pharmacology</topic><topic>Gold - chemistry</topic><topic>Gold - metabolism</topic><topic>Gold - toxicity</topic><topic>Humans</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - toxicity</topic><topic>Particle Size</topic><topic>Protein Binding - drug effects</topic><topic>Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maiorano, Gabriele</creatorcontrib><creatorcontrib>Sabella, Stefania</creatorcontrib><creatorcontrib>Sorce, Barbara</creatorcontrib><creatorcontrib>Brunetti, Virgilio</creatorcontrib><creatorcontrib>Malvindi, Maria Ada</creatorcontrib><creatorcontrib>Cingolani, Roberto</creatorcontrib><creatorcontrib>Pompa, Pier Paolo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>ACS nano</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maiorano, Gabriele</au><au>Sabella, Stefania</au><au>Sorce, Barbara</au><au>Brunetti, Virgilio</au><au>Malvindi, Maria Ada</au><au>Cingolani, Roberto</au><au>Pompa, Pier Paolo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Cell Culture Media on the Dynamic Formation of Protein−Nanoparticle Complexes and Influence on the Cellular Response</atitle><jtitle>ACS nano</jtitle><addtitle>ACS Nano</addtitle><date>2010-12-28</date><risdate>2010</risdate><volume>4</volume><issue>12</issue><spage>7481</spage><epage>7491</epage><pages>7481-7491</pages><issn>1936-0851</issn><eissn>1936-086X</eissn><abstract>The development of appropriate in vitro protocols to assess the potential toxicity of the ever expanding range of nanoparticles represents a challenging issue, because of the rapid changes of their intrinsic physicochemical properties (size, shape, reactivity, surface area, etc.) upon dispersion in biological fluids. Dynamic formation of protein coating around nanoparticles is a key molecular event, which may strongly impact the biological response in nanotoxicological tests. In this work, by using citrate-capped gold nanoparticles (AuNPs) of different sizes as a model, we show, by several spectroscopic techniques (dynamic light scattering, UV−visible, plasmon resonance light scattering), that proteins−NP interactions are differently mediated by two widely used cellular media (i.e., Dulbecco Modified Eagle’s medium (DMEM) and Roswell Park Memorial Institute medium (RPMI), supplemented with fetal bovine serum). We found that, while DMEM elicits the formation of a large time-dependent protein corona, RPMI shows different dynamics with reduced protein coating. Characterization of these nanobioentities was also performed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and mass spectroscopy, revealing that the average composition of protein corona does not reflect the relative abundance of serum proteins. To evaluate the biological impact of such hybrid bionanostructures, several comparative viability assays onto two cell lines (HeLa and U937) were carried out in the two media, in the presence of 15 nm AuNPs. We observed that proteins/NP complexes formed in RPMI are more abundantly internalized in cells as compared to DMEM, overall exerting higher cytotoxic effects. These results show that, beyond an in-depth NPs characterization before cellular experiments, a detailed understanding of the effects elicited by cell culture media on NPs is crucial for standardized nanotoxicology tests.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>21082814</pmid><doi>10.1021/nn101557e</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1936-0851 |
| ispartof | ACS nano, 2010-12, Vol.4 (12), p.7481-7491 |
| issn | 1936-0851 1936-086X |
| language | eng |
| recordid | cdi_crossref_primary_10_1021_nn101557e |
| source | MEDLINE; American Chemical Society Journals |
| subjects | Animals Cattle Cell Culture Techniques - methods Cell Line, Tumor Cell Proliferation - drug effects Citrates - chemistry Culture Media - pharmacology Gold - chemistry Gold - metabolism Gold - toxicity Humans Metal Nanoparticles - chemistry Nanoparticles - chemistry Nanoparticles - toxicity Particle Size Protein Binding - drug effects Proteins - metabolism |
| title | Effects of Cell Culture Media on the Dynamic Formation of Protein−Nanoparticle Complexes and Influence on the Cellular Response |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T13%3A29%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-acs_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effects%20of%20Cell%20Culture%20Media%20on%20the%20Dynamic%20Formation%20of%20Protein%E2%88%92Nanoparticle%20Complexes%20and%20Influence%20on%20the%20Cellular%20Response&rft.jtitle=ACS%20nano&rft.au=Maiorano,%20Gabriele&rft.date=2010-12-28&rft.volume=4&rft.issue=12&rft.spage=7481&rft.epage=7491&rft.pages=7481-7491&rft.issn=1936-0851&rft.eissn=1936-086X&rft_id=info:doi/10.1021/nn101557e&rft_dat=%3Cacs_cross%3Ef12023682%3C/acs_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/21082814&rfr_iscdi=true |