Silver/Dendrimer Nanocomposites as Biomarkers: Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection
We have synthesized water-soluble, biocompatible, fluorescent, and stable silver/dendrimer nanocomposites that exhibit a potential for in vitro cell labeling. Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous sil...
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Veröffentlicht in: | Nano letters 2005-11, Vol.5 (11), p.2123-2130 |
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description | We have synthesized water-soluble, biocompatible, fluorescent, and stable silver/dendrimer nanocomposites that exhibit a potential for in vitro cell labeling. Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous silver(I)−dendrimer complexes (with the molar ratio of 25 Ag+ per dendrimer) at the biologic pH of 7.4. Conversion of silver(I)−dendrimer complexes into dendrimer nanocomposites was achieved by irradiating the solutions with UV light to reduce the bound Ag+ cations to zerovalent Ag0 atoms, which were simultaneously trapped in the dendrimer network, resulting in the formation of {(Ag0)25−PAMAM_E5.NH2}, {(Ag0)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} dendrimer nanocomposites (DNC), respectively. The silver−DNCs were characterized by means of UV−vis, fluorescence spectroscopy, dynamic light-scattering, zeta potential measurements, high-resolution transmission electron microscopy, X-ray energy dispersive spectroscopy, and selected area electron diffraction. The cytotoxicity of dendrimers and related silver nanocomposites was evaluated using an XTT colorimetric assay of cellular viability. The cellular uptake of nanoparticles was examined by transmission electron and confocal microscopy. Results indicate that {(Ag0)25−PAMAM_E5.NH2}, {(Ag0-)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} form primarily single particles with diameters between 3 and 7 nm. The dendrimer nanocomposites are fluorescent, and their surface charge, cellular internalization, toxicity, and cell labeling capabilities are determined by the surface functionalities of dendrimer templates. The {(Ag0)25−PAMAM_E5.NH2} and {(Ag0)25−PAMAM_E5.NSAH} nanocomposites exhibit potential application as cell biomarkers. |
doi_str_mv | 10.1021/nl051077u |
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Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous silver(I)−dendrimer complexes (with the molar ratio of 25 Ag+ per dendrimer) at the biologic pH of 7.4. Conversion of silver(I)−dendrimer complexes into dendrimer nanocomposites was achieved by irradiating the solutions with UV light to reduce the bound Ag+ cations to zerovalent Ag0 atoms, which were simultaneously trapped in the dendrimer network, resulting in the formation of {(Ag0)25−PAMAM_E5.NH2}, {(Ag0)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} dendrimer nanocomposites (DNC), respectively. The silver−DNCs were characterized by means of UV−vis, fluorescence spectroscopy, dynamic light-scattering, zeta potential measurements, high-resolution transmission electron microscopy, X-ray energy dispersive spectroscopy, and selected area electron diffraction. The cytotoxicity of dendrimers and related silver nanocomposites was evaluated using an XTT colorimetric assay of cellular viability. The cellular uptake of nanoparticles was examined by transmission electron and confocal microscopy. Results indicate that {(Ag0)25−PAMAM_E5.NH2}, {(Ag0-)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} form primarily single particles with diameters between 3 and 7 nm. The dendrimer nanocomposites are fluorescent, and their surface charge, cellular internalization, toxicity, and cell labeling capabilities are determined by the surface functionalities of dendrimer templates. The {(Ag0)25−PAMAM_E5.NH2} and {(Ag0)25−PAMAM_E5.NSAH} nanocomposites exhibit potential application as cell biomarkers.</description><identifier>ISSN: 1530-6984</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/nl051077u</identifier><identifier>PMID: 16277438</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Applied sciences ; Biocompatible Materials ; Biomarkers ; Condensed matter: structure, mechanical and thermal properties ; Dendrimers - analysis ; Dendrimers - chemical synthesis ; Dendrimers - toxicity ; Electronics ; Exact sciences and technology ; Humans ; Mice ; Microelectronic fabrication (materials and surfaces technology) ; Microscopy, Electron ; Nanoscale materials: clusters, nanoparticles, nanotubes, and nanocrystals ; Nanostructures - analysis ; Nanostructures - chemistry ; Nanostructures - toxicity ; Nanotechnology ; NIH 3T3 Cells ; Physics ; Polyamines - analysis ; Polyamines - chemical synthesis ; Polyamines - toxicity ; Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices ; Silver - analysis ; Silver - chemistry ; Silver - toxicity ; Structure of solids and liquids; crystallography ; U937 Cells</subject><ispartof>Nano letters, 2005-11, Vol.5 (11), p.2123-2130</ispartof><rights>Copyright © 2005 American Chemical Society</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a343t-e6cdcad294ea40d99443a7ab74ae3f6a272180fd2eea19954dace47712679db3</citedby><cites>FETCH-LOGICAL-a343t-e6cdcad294ea40d99443a7ab74ae3f6a272180fd2eea19954dace47712679db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/nl051077u$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/nl051077u$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2764,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17293656$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16277438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lesniak, Wojciech</creatorcontrib><creatorcontrib>Bielinska, Anna U</creatorcontrib><creatorcontrib>Sun, Kai</creatorcontrib><creatorcontrib>Janczak, Katarzyna W</creatorcontrib><creatorcontrib>Shi, Xiangyang</creatorcontrib><creatorcontrib>Baker, James R</creatorcontrib><creatorcontrib>Balogh, Lajos P</creatorcontrib><title>Silver/Dendrimer Nanocomposites as Biomarkers: Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection</title><title>Nano letters</title><addtitle>Nano Lett</addtitle><description>We have synthesized water-soluble, biocompatible, fluorescent, and stable silver/dendrimer nanocomposites that exhibit a potential for in vitro cell labeling. Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous silver(I)−dendrimer complexes (with the molar ratio of 25 Ag+ per dendrimer) at the biologic pH of 7.4. Conversion of silver(I)−dendrimer complexes into dendrimer nanocomposites was achieved by irradiating the solutions with UV light to reduce the bound Ag+ cations to zerovalent Ag0 atoms, which were simultaneously trapped in the dendrimer network, resulting in the formation of {(Ag0)25−PAMAM_E5.NH2}, {(Ag0)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} dendrimer nanocomposites (DNC), respectively. The silver−DNCs were characterized by means of UV−vis, fluorescence spectroscopy, dynamic light-scattering, zeta potential measurements, high-resolution transmission electron microscopy, X-ray energy dispersive spectroscopy, and selected area electron diffraction. The cytotoxicity of dendrimers and related silver nanocomposites was evaluated using an XTT colorimetric assay of cellular viability. The cellular uptake of nanoparticles was examined by transmission electron and confocal microscopy. Results indicate that {(Ag0)25−PAMAM_E5.NH2}, {(Ag0-)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} form primarily single particles with diameters between 3 and 7 nm. The dendrimer nanocomposites are fluorescent, and their surface charge, cellular internalization, toxicity, and cell labeling capabilities are determined by the surface functionalities of dendrimer templates. The {(Ag0)25−PAMAM_E5.NH2} and {(Ag0)25−PAMAM_E5.NSAH} nanocomposites exhibit potential application as cell biomarkers.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Biocompatible Materials</subject><subject>Biomarkers</subject><subject>Condensed matter: structure, mechanical and thermal properties</subject><subject>Dendrimers - analysis</subject><subject>Dendrimers - chemical synthesis</subject><subject>Dendrimers - toxicity</subject><subject>Electronics</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Mice</subject><subject>Microelectronic fabrication (materials and surfaces technology)</subject><subject>Microscopy, Electron</subject><subject>Nanoscale materials: clusters, nanoparticles, nanotubes, and nanocrystals</subject><subject>Nanostructures - analysis</subject><subject>Nanostructures - chemistry</subject><subject>Nanostructures - toxicity</subject><subject>Nanotechnology</subject><subject>NIH 3T3 Cells</subject><subject>Physics</subject><subject>Polyamines - analysis</subject><subject>Polyamines - chemical synthesis</subject><subject>Polyamines - toxicity</subject><subject>Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices</subject><subject>Silver - analysis</subject><subject>Silver - chemistry</subject><subject>Silver - toxicity</subject><subject>Structure of solids and liquids; crystallography</subject><subject>U937 Cells</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0EtLAzEQB_Agiu-DX0By8SBYzWs3jTetTxA9WLwu02QWo9ukJFuxXvTq1_STuGKxF08zDD9mmD8hO5wdcib4UWhYwZnW0yWyzgvJeqUxYvmv76s1spHzE2PMyIKtkjVeCq2V7K-T93vfvGA6OsPgkh9jorcQoo3jScy-xUwh01Mfx5CeMeXjr49PegGj5C20PoYDOniEBLbF5N_mEx_og29TpMP46q1vZwcUgqPXoe0gNs20gUTPsEX747fISg1Nxu153STDi_Ph4Kp3c3d5PTi56YFUsu1haZ0FJ4xCUMwZo5QEDSOtAGVdgtCC91ntBCJwYwrlultKay5KbdxIbpL937U2xZwT1tWk-xbSrOKs-smw-suws7u_djIdjdEt5Dy0DuzNAWQLTZ0gWJ8XTgsjy6JcOLC5eorTFLoP_zn4DQmsiRU</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Lesniak, Wojciech</creator><creator>Bielinska, Anna U</creator><creator>Sun, Kai</creator><creator>Janczak, Katarzyna W</creator><creator>Shi, Xiangyang</creator><creator>Baker, James R</creator><creator>Balogh, Lajos P</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20051101</creationdate><title>Silver/Dendrimer Nanocomposites as Biomarkers: Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection</title><author>Lesniak, Wojciech ; Bielinska, Anna U ; Sun, Kai ; Janczak, Katarzyna W ; Shi, Xiangyang ; Baker, James R ; Balogh, Lajos P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a343t-e6cdcad294ea40d99443a7ab74ae3f6a272180fd2eea19954dace47712679db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Biocompatible Materials</topic><topic>Biomarkers</topic><topic>Condensed matter: structure, mechanical and thermal properties</topic><topic>Dendrimers - analysis</topic><topic>Dendrimers - chemical synthesis</topic><topic>Dendrimers - toxicity</topic><topic>Electronics</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Mice</topic><topic>Microelectronic fabrication (materials and surfaces technology)</topic><topic>Microscopy, Electron</topic><topic>Nanoscale materials: clusters, nanoparticles, nanotubes, and nanocrystals</topic><topic>Nanostructures - analysis</topic><topic>Nanostructures - chemistry</topic><topic>Nanostructures - toxicity</topic><topic>Nanotechnology</topic><topic>NIH 3T3 Cells</topic><topic>Physics</topic><topic>Polyamines - analysis</topic><topic>Polyamines - chemical synthesis</topic><topic>Polyamines - toxicity</topic><topic>Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices</topic><topic>Silver - analysis</topic><topic>Silver - chemistry</topic><topic>Silver - toxicity</topic><topic>Structure of solids and liquids; crystallography</topic><topic>U937 Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lesniak, Wojciech</creatorcontrib><creatorcontrib>Bielinska, Anna U</creatorcontrib><creatorcontrib>Sun, Kai</creatorcontrib><creatorcontrib>Janczak, Katarzyna W</creatorcontrib><creatorcontrib>Shi, Xiangyang</creatorcontrib><creatorcontrib>Baker, James R</creatorcontrib><creatorcontrib>Balogh, Lajos P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lesniak, Wojciech</au><au>Bielinska, Anna U</au><au>Sun, Kai</au><au>Janczak, Katarzyna W</au><au>Shi, Xiangyang</au><au>Baker, James R</au><au>Balogh, Lajos P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silver/Dendrimer Nanocomposites as Biomarkers: Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2005-11-01</date><risdate>2005</risdate><volume>5</volume><issue>11</issue><spage>2123</spage><epage>2130</epage><pages>2123-2130</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>We have synthesized water-soluble, biocompatible, fluorescent, and stable silver/dendrimer nanocomposites that exhibit a potential for in vitro cell labeling. Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous silver(I)−dendrimer complexes (with the molar ratio of 25 Ag+ per dendrimer) at the biologic pH of 7.4. Conversion of silver(I)−dendrimer complexes into dendrimer nanocomposites was achieved by irradiating the solutions with UV light to reduce the bound Ag+ cations to zerovalent Ag0 atoms, which were simultaneously trapped in the dendrimer network, resulting in the formation of {(Ag0)25−PAMAM_E5.NH2}, {(Ag0)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} dendrimer nanocomposites (DNC), respectively. The silver−DNCs were characterized by means of UV−vis, fluorescence spectroscopy, dynamic light-scattering, zeta potential measurements, high-resolution transmission electron microscopy, X-ray energy dispersive spectroscopy, and selected area electron diffraction. The cytotoxicity of dendrimers and related silver nanocomposites was evaluated using an XTT colorimetric assay of cellular viability. The cellular uptake of nanoparticles was examined by transmission electron and confocal microscopy. Results indicate that {(Ag0)25−PAMAM_E5.NH2}, {(Ag0-)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} form primarily single particles with diameters between 3 and 7 nm. The dendrimer nanocomposites are fluorescent, and their surface charge, cellular internalization, toxicity, and cell labeling capabilities are determined by the surface functionalities of dendrimer templates. The {(Ag0)25−PAMAM_E5.NH2} and {(Ag0)25−PAMAM_E5.NSAH} nanocomposites exhibit potential application as cell biomarkers.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>16277438</pmid><doi>10.1021/nl051077u</doi><tpages>8</tpages></addata></record> |
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subjects | Animals Applied sciences Biocompatible Materials Biomarkers Condensed matter: structure, mechanical and thermal properties Dendrimers - analysis Dendrimers - chemical synthesis Dendrimers - toxicity Electronics Exact sciences and technology Humans Mice Microelectronic fabrication (materials and surfaces technology) Microscopy, Electron Nanoscale materials: clusters, nanoparticles, nanotubes, and nanocrystals Nanostructures - analysis Nanostructures - chemistry Nanostructures - toxicity Nanotechnology NIH 3T3 Cells Physics Polyamines - analysis Polyamines - chemical synthesis Polyamines - toxicity Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices Silver - analysis Silver - chemistry Silver - toxicity Structure of solids and liquids crystallography U937 Cells |
title | Silver/Dendrimer Nanocomposites as Biomarkers: Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection |
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