Silver/Dendrimer Nanocomposites as Biomarkers:  Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection

We have synthesized water-soluble, biocompatible, fluorescent, and stable silver/dendrimer nanocomposites that exhibit a potential for in vitro cell labeling. Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous sil...

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Veröffentlicht in:Nano letters 2005-11, Vol.5 (11), p.2123-2130
Hauptverfasser: Lesniak, Wojciech, Bielinska, Anna U, Sun, Kai, Janczak, Katarzyna W, Shi, Xiangyang, Baker, James R, Balogh, Lajos P
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container_end_page 2130
container_issue 11
container_start_page 2123
container_title Nano letters
container_volume 5
creator Lesniak, Wojciech
Bielinska, Anna U
Sun, Kai
Janczak, Katarzyna W
Shi, Xiangyang
Baker, James R
Balogh, Lajos P
description We have synthesized water-soluble, biocompatible, fluorescent, and stable silver/dendrimer nanocomposites that exhibit a potential for in vitro cell labeling. Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous silver(I)−dendrimer complexes (with the molar ratio of 25 Ag+ per dendrimer) at the biologic pH of 7.4. Conversion of silver(I)−dendrimer complexes into dendrimer nanocomposites was achieved by irradiating the solutions with UV light to reduce the bound Ag+ cations to zerovalent Ag0 atoms, which were simultaneously trapped in the dendrimer network, resulting in the formation of {(Ag0)25−PAMAM_E5.NH2}, {(Ag0)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} dendrimer nanocomposites (DNC), respectively. The silver−DNCs were characterized by means of UV−vis, fluorescence spectroscopy, dynamic light-scattering, zeta potential measurements, high-resolution transmission electron microscopy, X-ray energy dispersive spectroscopy, and selected area electron diffraction. The cytotoxicity of dendrimers and related silver nanocomposites was evaluated using an XTT colorimetric assay of cellular viability. The cellular uptake of nanoparticles was examined by transmission electron and confocal microscopy. Results indicate that {(Ag0)25−PAMAM_E5.NH2}, {(Ag0-)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} form primarily single particles with diameters between 3 and 7 nm. The dendrimer nanocomposites are fluorescent, and their surface charge, cellular internalization, toxicity, and cell labeling capabilities are determined by the surface functionalities of dendrimer templates. The {(Ag0)25−PAMAM_E5.NH2} and {(Ag0)25−PAMAM_E5.NSAH} nanocomposites exhibit potential application as cell biomarkers.
doi_str_mv 10.1021/nl051077u
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Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous silver(I)−dendrimer complexes (with the molar ratio of 25 Ag+ per dendrimer) at the biologic pH of 7.4. Conversion of silver(I)−dendrimer complexes into dendrimer nanocomposites was achieved by irradiating the solutions with UV light to reduce the bound Ag+ cations to zerovalent Ag0 atoms, which were simultaneously trapped in the dendrimer network, resulting in the formation of {(Ag0)25−PAMAM_E5.NH2}, {(Ag0)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} dendrimer nanocomposites (DNC), respectively. The silver−DNCs were characterized by means of UV−vis, fluorescence spectroscopy, dynamic light-scattering, zeta potential measurements, high-resolution transmission electron microscopy, X-ray energy dispersive spectroscopy, and selected area electron diffraction. The cytotoxicity of dendrimers and related silver nanocomposites was evaluated using an XTT colorimetric assay of cellular viability. The cellular uptake of nanoparticles was examined by transmission electron and confocal microscopy. Results indicate that {(Ag0)25−PAMAM_E5.NH2}, {(Ag0-)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} form primarily single particles with diameters between 3 and 7 nm. The dendrimer nanocomposites are fluorescent, and their surface charge, cellular internalization, toxicity, and cell labeling capabilities are determined by the surface functionalities of dendrimer templates. The {(Ag0)25−PAMAM_E5.NH2} and {(Ag0)25−PAMAM_E5.NSAH} nanocomposites exhibit potential application as cell biomarkers.</description><identifier>ISSN: 1530-6984</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/nl051077u</identifier><identifier>PMID: 16277438</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Applied sciences ; Biocompatible Materials ; Biomarkers ; Condensed matter: structure, mechanical and thermal properties ; Dendrimers - analysis ; Dendrimers - chemical synthesis ; Dendrimers - toxicity ; Electronics ; Exact sciences and technology ; Humans ; Mice ; Microelectronic fabrication (materials and surfaces technology) ; Microscopy, Electron ; Nanoscale materials: clusters, nanoparticles, nanotubes, and nanocrystals ; Nanostructures - analysis ; Nanostructures - chemistry ; Nanostructures - toxicity ; Nanotechnology ; NIH 3T3 Cells ; Physics ; Polyamines - analysis ; Polyamines - chemical synthesis ; Polyamines - toxicity ; Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices ; Silver - analysis ; Silver - chemistry ; Silver - toxicity ; Structure of solids and liquids; crystallography ; U937 Cells</subject><ispartof>Nano letters, 2005-11, Vol.5 (11), p.2123-2130</ispartof><rights>Copyright © 2005 American Chemical Society</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a343t-e6cdcad294ea40d99443a7ab74ae3f6a272180fd2eea19954dace47712679db3</citedby><cites>FETCH-LOGICAL-a343t-e6cdcad294ea40d99443a7ab74ae3f6a272180fd2eea19954dace47712679db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/nl051077u$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/nl051077u$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2764,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=17293656$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16277438$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lesniak, Wojciech</creatorcontrib><creatorcontrib>Bielinska, Anna U</creatorcontrib><creatorcontrib>Sun, Kai</creatorcontrib><creatorcontrib>Janczak, Katarzyna W</creatorcontrib><creatorcontrib>Shi, Xiangyang</creatorcontrib><creatorcontrib>Baker, James R</creatorcontrib><creatorcontrib>Balogh, Lajos P</creatorcontrib><title>Silver/Dendrimer Nanocomposites as Biomarkers:  Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection</title><title>Nano letters</title><addtitle>Nano Lett</addtitle><description>We have synthesized water-soluble, biocompatible, fluorescent, and stable silver/dendrimer nanocomposites that exhibit a potential for in vitro cell labeling. Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous silver(I)−dendrimer complexes (with the molar ratio of 25 Ag+ per dendrimer) at the biologic pH of 7.4. Conversion of silver(I)−dendrimer complexes into dendrimer nanocomposites was achieved by irradiating the solutions with UV light to reduce the bound Ag+ cations to zerovalent Ag0 atoms, which were simultaneously trapped in the dendrimer network, resulting in the formation of {(Ag0)25−PAMAM_E5.NH2}, {(Ag0)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} dendrimer nanocomposites (DNC), respectively. The silver−DNCs were characterized by means of UV−vis, fluorescence spectroscopy, dynamic light-scattering, zeta potential measurements, high-resolution transmission electron microscopy, X-ray energy dispersive spectroscopy, and selected area electron diffraction. The cytotoxicity of dendrimers and related silver nanocomposites was evaluated using an XTT colorimetric assay of cellular viability. The cellular uptake of nanoparticles was examined by transmission electron and confocal microscopy. Results indicate that {(Ag0)25−PAMAM_E5.NH2}, {(Ag0-)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} form primarily single particles with diameters between 3 and 7 nm. The dendrimer nanocomposites are fluorescent, and their surface charge, cellular internalization, toxicity, and cell labeling capabilities are determined by the surface functionalities of dendrimer templates. The {(Ag0)25−PAMAM_E5.NH2} and {(Ag0)25−PAMAM_E5.NSAH} nanocomposites exhibit potential application as cell biomarkers.</description><subject>Animals</subject><subject>Applied sciences</subject><subject>Biocompatible Materials</subject><subject>Biomarkers</subject><subject>Condensed matter: structure, mechanical and thermal properties</subject><subject>Dendrimers - analysis</subject><subject>Dendrimers - chemical synthesis</subject><subject>Dendrimers - toxicity</subject><subject>Electronics</subject><subject>Exact sciences and technology</subject><subject>Humans</subject><subject>Mice</subject><subject>Microelectronic fabrication (materials and surfaces technology)</subject><subject>Microscopy, Electron</subject><subject>Nanoscale materials: clusters, nanoparticles, nanotubes, and nanocrystals</subject><subject>Nanostructures - analysis</subject><subject>Nanostructures - chemistry</subject><subject>Nanostructures - toxicity</subject><subject>Nanotechnology</subject><subject>NIH 3T3 Cells</subject><subject>Physics</subject><subject>Polyamines - analysis</subject><subject>Polyamines - chemical synthesis</subject><subject>Polyamines - toxicity</subject><subject>Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices</subject><subject>Silver - analysis</subject><subject>Silver - chemistry</subject><subject>Silver - toxicity</subject><subject>Structure of solids and liquids; crystallography</subject><subject>U937 Cells</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0EtLAzEQB_Agiu-DX0By8SBYzWs3jTetTxA9WLwu02QWo9ukJFuxXvTq1_STuGKxF08zDD9mmD8hO5wdcib4UWhYwZnW0yWyzgvJeqUxYvmv76s1spHzE2PMyIKtkjVeCq2V7K-T93vfvGA6OsPgkh9jorcQoo3jScy-xUwh01Mfx5CeMeXjr49PegGj5C20PoYDOniEBLbF5N_mEx_og29TpMP46q1vZwcUgqPXoe0gNs20gUTPsEX747fISg1Nxu153STDi_Ph4Kp3c3d5PTi56YFUsu1haZ0FJ4xCUMwZo5QEDSOtAGVdgtCC91ntBCJwYwrlultKay5KbdxIbpL937U2xZwT1tWk-xbSrOKs-smw-suws7u_djIdjdEt5Dy0DuzNAWQLTZ0gWJ8XTgsjy6JcOLC5eorTFLoP_zn4DQmsiRU</recordid><startdate>20051101</startdate><enddate>20051101</enddate><creator>Lesniak, Wojciech</creator><creator>Bielinska, Anna U</creator><creator>Sun, Kai</creator><creator>Janczak, Katarzyna W</creator><creator>Shi, Xiangyang</creator><creator>Baker, James R</creator><creator>Balogh, Lajos P</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20051101</creationdate><title>Silver/Dendrimer Nanocomposites as Biomarkers:  Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection</title><author>Lesniak, Wojciech ; Bielinska, Anna U ; Sun, Kai ; Janczak, Katarzyna W ; Shi, Xiangyang ; Baker, James R ; Balogh, Lajos P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a343t-e6cdcad294ea40d99443a7ab74ae3f6a272180fd2eea19954dace47712679db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Applied sciences</topic><topic>Biocompatible Materials</topic><topic>Biomarkers</topic><topic>Condensed matter: structure, mechanical and thermal properties</topic><topic>Dendrimers - analysis</topic><topic>Dendrimers - chemical synthesis</topic><topic>Dendrimers - toxicity</topic><topic>Electronics</topic><topic>Exact sciences and technology</topic><topic>Humans</topic><topic>Mice</topic><topic>Microelectronic fabrication (materials and surfaces technology)</topic><topic>Microscopy, Electron</topic><topic>Nanoscale materials: clusters, nanoparticles, nanotubes, and nanocrystals</topic><topic>Nanostructures - analysis</topic><topic>Nanostructures - chemistry</topic><topic>Nanostructures - toxicity</topic><topic>Nanotechnology</topic><topic>NIH 3T3 Cells</topic><topic>Physics</topic><topic>Polyamines - analysis</topic><topic>Polyamines - chemical synthesis</topic><topic>Polyamines - toxicity</topic><topic>Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices</topic><topic>Silver - analysis</topic><topic>Silver - chemistry</topic><topic>Silver - toxicity</topic><topic>Structure of solids and liquids; crystallography</topic><topic>U937 Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lesniak, Wojciech</creatorcontrib><creatorcontrib>Bielinska, Anna U</creatorcontrib><creatorcontrib>Sun, Kai</creatorcontrib><creatorcontrib>Janczak, Katarzyna W</creatorcontrib><creatorcontrib>Shi, Xiangyang</creatorcontrib><creatorcontrib>Baker, James R</creatorcontrib><creatorcontrib>Balogh, Lajos P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lesniak, Wojciech</au><au>Bielinska, Anna U</au><au>Sun, Kai</au><au>Janczak, Katarzyna W</au><au>Shi, Xiangyang</au><au>Baker, James R</au><au>Balogh, Lajos P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silver/Dendrimer Nanocomposites as Biomarkers:  Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2005-11-01</date><risdate>2005</risdate><volume>5</volume><issue>11</issue><spage>2123</spage><epage>2130</epage><pages>2123-2130</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>We have synthesized water-soluble, biocompatible, fluorescent, and stable silver/dendrimer nanocomposites that exhibit a potential for in vitro cell labeling. Amino-, hydroxyl-, and carboxyl-terminated ethylenediamine core generation 5 poly(amidoamine) dendrimers were utilized to prepare aqueous silver(I)−dendrimer complexes (with the molar ratio of 25 Ag+ per dendrimer) at the biologic pH of 7.4. Conversion of silver(I)−dendrimer complexes into dendrimer nanocomposites was achieved by irradiating the solutions with UV light to reduce the bound Ag+ cations to zerovalent Ag0 atoms, which were simultaneously trapped in the dendrimer network, resulting in the formation of {(Ag0)25−PAMAM_E5.NH2}, {(Ag0)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} dendrimer nanocomposites (DNC), respectively. The silver−DNCs were characterized by means of UV−vis, fluorescence spectroscopy, dynamic light-scattering, zeta potential measurements, high-resolution transmission electron microscopy, X-ray energy dispersive spectroscopy, and selected area electron diffraction. The cytotoxicity of dendrimers and related silver nanocomposites was evaluated using an XTT colorimetric assay of cellular viability. The cellular uptake of nanoparticles was examined by transmission electron and confocal microscopy. Results indicate that {(Ag0)25−PAMAM_E5.NH2}, {(Ag0-)25−PAMAM_E5.NGly}, and {(Ag0)25−PAMAM_E5.NSAH} form primarily single particles with diameters between 3 and 7 nm. The dendrimer nanocomposites are fluorescent, and their surface charge, cellular internalization, toxicity, and cell labeling capabilities are determined by the surface functionalities of dendrimer templates. The {(Ag0)25−PAMAM_E5.NH2} and {(Ag0)25−PAMAM_E5.NSAH} nanocomposites exhibit potential application as cell biomarkers.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>16277438</pmid><doi>10.1021/nl051077u</doi><tpages>8</tpages></addata></record>
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source MEDLINE; ACS Publications
subjects Animals
Applied sciences
Biocompatible Materials
Biomarkers
Condensed matter: structure, mechanical and thermal properties
Dendrimers - analysis
Dendrimers - chemical synthesis
Dendrimers - toxicity
Electronics
Exact sciences and technology
Humans
Mice
Microelectronic fabrication (materials and surfaces technology)
Microscopy, Electron
Nanoscale materials: clusters, nanoparticles, nanotubes, and nanocrystals
Nanostructures - analysis
Nanostructures - chemistry
Nanostructures - toxicity
Nanotechnology
NIH 3T3 Cells
Physics
Polyamines - analysis
Polyamines - chemical synthesis
Polyamines - toxicity
Semiconductor electronics. Microelectronics. Optoelectronics. Solid state devices
Silver - analysis
Silver - chemistry
Silver - toxicity
Structure of solids and liquids
crystallography
U937 Cells
title Silver/Dendrimer Nanocomposites as Biomarkers:  Fabrication, Characterization, in Vitro Toxicity, and Intracellular Detection
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