Using Nanografting to Achieve Directed Assembly of de novo Designed Metalloproteins on Gold

Using Nanografting to Achieve Directed Assembly of de novo Designed Metalloproteins on Gold

Parallel three-helix bundle metalloproteins incorporating C-terminal thiol groups have been designed to orient vertically on a gold(111) surface. A small area of pre-assembled octadecanethiol was exchanged with the proteins under the action of an AFM tip. The resulting “nanografted” metalloprotein w...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Nano letters 2003-04, Vol.3 (4), p.425-429
Hauptverfasser: Case, Martin A, McLendon, George L, Hu, Ying, Vanderlick, T. Kyle, Scoles, Giacinto
Format: Artikel
Sprache:eng
Schlagworte:
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 429
container_issue 4
container_start_page 425
container_title Nano letters
container_volume 3
creator Case, Martin A
McLendon, George L
Hu, Ying
Vanderlick, T. Kyle
Scoles, Giacinto
description Parallel three-helix bundle metalloproteins incorporating C-terminal thiol groups have been designed to orient vertically on a gold(111) surface. A small area of pre-assembled octadecanethiol was exchanged with the proteins under the action of an AFM tip. The resulting “nanografted” metalloprotein was imaged at low applied force. The measured height of the octadecanethiol monolayer was 2.2(1) nm and the mean height above this of the grafted metalloprotein layer was 3.1(4) nm. Thus the total height of the grafted metalloprotein is 5.3(4) nm. The predicted height of the vertically oriented metalloprotein is 5.2 nm. We take this as good evidence for spatial control of monolayer assembly of three-helix bundles with predictable orientation.
doi_str_mv 10.1021/nl025795h
format Article
fullrecord <record><control><sourceid>acs_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1021_nl025795h</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>a404605567</sourcerecordid><originalsourceid>FETCH-LOGICAL-a287t-137106f77675789d72521dbc817caffdd18cc6a754ba0da939ea3cd9f8fffee43</originalsourceid><addsrcrecordid>eNptkE1PAjEQhhujiYge_Ae9ePCw2u5ut-2RgKIJ6kVOHjZDP6CktKRdSfj3LsHgxdPMZJ55k3kQuqXkgZKSPgZPSsYlW52hAWUVKRopy_NTL-pLdJXzmhAiK0YG6GueXVjidwhxmcB2h6GLeKRWzuwMnrhkVGc0HuVsNgu_x9FibXCIu4gnJrtl6JdvpgPv4zbFzriQcQx4Gr2-RhcWfDY3v3WI5s9Pn-OXYvYxfR2PZgWUgncFrTgljeW84YwLqXnJSqoXSlCuwFqtqVCqAc7qBRANspIGKqWlFdZaY-pqiO6PuSrFnJOx7Ta5DaR9S0l7sNKerPTs3ZHdQlbgbYKgXP47qHlNRC_rxIHK7Tp-p9B_8E_eD4BabwI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Using Nanografting to Achieve Directed Assembly of de novo Designed Metalloproteins on Gold</title><source>ACS Publications</source><creator>Case, Martin A ; McLendon, George L ; Hu, Ying ; Vanderlick, T. Kyle ; Scoles, Giacinto</creator><creatorcontrib>Case, Martin A ; McLendon, George L ; Hu, Ying ; Vanderlick, T. Kyle ; Scoles, Giacinto</creatorcontrib><description>Parallel three-helix bundle metalloproteins incorporating C-terminal thiol groups have been designed to orient vertically on a gold(111) surface. A small area of pre-assembled octadecanethiol was exchanged with the proteins under the action of an AFM tip. The resulting “nanografted” metalloprotein was imaged at low applied force. The measured height of the octadecanethiol monolayer was 2.2(1) nm and the mean height above this of the grafted metalloprotein layer was 3.1(4) nm. Thus the total height of the grafted metalloprotein is 5.3(4) nm. The predicted height of the vertically oriented metalloprotein is 5.2 nm. We take this as good evidence for spatial control of monolayer assembly of three-helix bundles with predictable orientation.</description><identifier>ISSN: 1530-6984</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/nl025795h</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical biochemistry: general aspects, technics, instrumentation ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology</subject><ispartof>Nano letters, 2003-04, Vol.3 (4), p.425-429</ispartof><rights>Copyright © 2003 American Chemical Society</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a287t-137106f77675789d72521dbc817caffdd18cc6a754ba0da939ea3cd9f8fffee43</citedby><cites>FETCH-LOGICAL-a287t-137106f77675789d72521dbc817caffdd18cc6a754ba0da939ea3cd9f8fffee43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/nl025795h$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/nl025795h$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=14740815$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Case, Martin A</creatorcontrib><creatorcontrib>McLendon, George L</creatorcontrib><creatorcontrib>Hu, Ying</creatorcontrib><creatorcontrib>Vanderlick, T. Kyle</creatorcontrib><creatorcontrib>Scoles, Giacinto</creatorcontrib><title>Using Nanografting to Achieve Directed Assembly of de novo Designed Metalloproteins on Gold</title><title>Nano letters</title><addtitle>Nano Lett</addtitle><description>Parallel three-helix bundle metalloproteins incorporating C-terminal thiol groups have been designed to orient vertically on a gold(111) surface. A small area of pre-assembled octadecanethiol was exchanged with the proteins under the action of an AFM tip. The resulting “nanografted” metalloprotein was imaged at low applied force. The measured height of the octadecanethiol monolayer was 2.2(1) nm and the mean height above this of the grafted metalloprotein layer was 3.1(4) nm. Thus the total height of the grafted metalloprotein is 5.3(4) nm. The predicted height of the vertically oriented metalloprotein is 5.2 nm. We take this as good evidence for spatial control of monolayer assembly of three-helix bundles with predictable orientation.</description><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNptkE1PAjEQhhujiYge_Ae9ePCw2u5ut-2RgKIJ6kVOHjZDP6CktKRdSfj3LsHgxdPMZJ55k3kQuqXkgZKSPgZPSsYlW52hAWUVKRopy_NTL-pLdJXzmhAiK0YG6GueXVjidwhxmcB2h6GLeKRWzuwMnrhkVGc0HuVsNgu_x9FibXCIu4gnJrtl6JdvpgPv4zbFzriQcQx4Gr2-RhcWfDY3v3WI5s9Pn-OXYvYxfR2PZgWUgncFrTgljeW84YwLqXnJSqoXSlCuwFqtqVCqAc7qBRANspIGKqWlFdZaY-pqiO6PuSrFnJOx7Ta5DaR9S0l7sNKerPTs3ZHdQlbgbYKgXP47qHlNRC_rxIHK7Tp-p9B_8E_eD4BabwI</recordid><startdate>20030401</startdate><enddate>20030401</enddate><creator>Case, Martin A</creator><creator>McLendon, George L</creator><creator>Hu, Ying</creator><creator>Vanderlick, T. Kyle</creator><creator>Scoles, Giacinto</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20030401</creationdate><title>Using Nanografting to Achieve Directed Assembly of de novo Designed Metalloproteins on Gold</title><author>Case, Martin A ; McLendon, George L ; Hu, Ying ; Vanderlick, T. Kyle ; Scoles, Giacinto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a287t-137106f77675789d72521dbc817caffdd18cc6a754ba0da939ea3cd9f8fffee43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Analytical biochemistry: general aspects, technics, instrumentation</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Case, Martin A</creatorcontrib><creatorcontrib>McLendon, George L</creatorcontrib><creatorcontrib>Hu, Ying</creatorcontrib><creatorcontrib>Vanderlick, T. Kyle</creatorcontrib><creatorcontrib>Scoles, Giacinto</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Case, Martin A</au><au>McLendon, George L</au><au>Hu, Ying</au><au>Vanderlick, T. Kyle</au><au>Scoles, Giacinto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Using Nanografting to Achieve Directed Assembly of de novo Designed Metalloproteins on Gold</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2003-04-01</date><risdate>2003</risdate><volume>3</volume><issue>4</issue><spage>425</spage><epage>429</epage><pages>425-429</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>Parallel three-helix bundle metalloproteins incorporating C-terminal thiol groups have been designed to orient vertically on a gold(111) surface. A small area of pre-assembled octadecanethiol was exchanged with the proteins under the action of an AFM tip. The resulting “nanografted” metalloprotein was imaged at low applied force. The measured height of the octadecanethiol monolayer was 2.2(1) nm and the mean height above this of the grafted metalloprotein layer was 3.1(4) nm. Thus the total height of the grafted metalloprotein is 5.3(4) nm. The predicted height of the vertically oriented metalloprotein is 5.2 nm. We take this as good evidence for spatial control of monolayer assembly of three-helix bundles with predictable orientation.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/nl025795h</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1530-6984
ispartof Nano letters, 2003-04, Vol.3 (4), p.425-429
issn 1530-6984
1530-6992
language eng
recordid cdi_crossref_primary_10_1021_nl025795h
source ACS Publications
subjects Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
title Using Nanografting to Achieve Directed Assembly of de novo Designed Metalloproteins on Gold
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T05%3A56%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-acs_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Using%20Nanografting%20to%20Achieve%20Directed%20Assembly%20of%20de%20novo%20Designed%20Metalloproteins%20on%20Gold&rft.jtitle=Nano%20letters&rft.au=Case,%20Martin%20A&rft.date=2003-04-01&rft.volume=3&rft.issue=4&rft.spage=425&rft.epage=429&rft.pages=425-429&rft.issn=1530-6984&rft.eissn=1530-6992&rft_id=info:doi/10.1021/nl025795h&rft_dat=%3Cacs_cross%3Ea404605567%3C/acs_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true