Chitosan as a Removing Agent of β-Lactoglobulin from Membrane Models
Many chitosan biological activities depend on the interaction with biomembranes, but so far it has not been possible to obtain molecular-level evidence of chitosan action. In this article, we employ Langmuir phospholipid monolayers as cell membrane models and show that chitosan is able to remove β-l...
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| Veröffentlicht in: | Langmuir 2008-04, Vol.24 (8), p.4150-4156 |
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| creator | Caseli, Luciano Pavinatto, Felippe J Nobre, Thatyane Morimoto Zaniquelli, Maria E. D Viitala, Tapani Oliveira, Osvaldo N |
| description | Many chitosan biological activities depend on the interaction with biomembranes, but so far it has not been possible to obtain molecular-level evidence of chitosan action. In this article, we employ Langmuir phospholipid monolayers as cell membrane models and show that chitosan is able to remove β-lactoglobulin (BLG) from negatively charged dimyristoyl phosphatidic acid (DMPA) and dipalmitoyl phosphatidyl glycerol (DPPG). This was shown with surface pressure isotherms and elasticity and PM-IRRAS measurements in the Langmuir monolayers, in addition to quartz crystal microbalance and fluorescence spectroscopy measurements for Langmuir−Blodgett (LB) films transferred onto solid substrates. Some specificity was noted in the removal action because chitosan was unable to remove BLG incorporated into neutral dipalmitoyl phosphatidyl choline (DPPC) and cholesterol monolayers and had no effect on horseradish peroxidase and urease interacting with DMPA. An obvious biological implication of these findings is to offer reasons that chitosan can remove BLG from lipophilic environments, as reported in the recent literature. |
| doi_str_mv | 10.1021/la7038762 |
| format | Article |
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D ; Viitala, Tapani ; Oliveira, Osvaldo N</creator><creatorcontrib>Caseli, Luciano ; Pavinatto, Felippe J ; Nobre, Thatyane Morimoto ; Zaniquelli, Maria E. D ; Viitala, Tapani ; Oliveira, Osvaldo N</creatorcontrib><description>Many chitosan biological activities depend on the interaction with biomembranes, but so far it has not been possible to obtain molecular-level evidence of chitosan action. In this article, we employ Langmuir phospholipid monolayers as cell membrane models and show that chitosan is able to remove β-lactoglobulin (BLG) from negatively charged dimyristoyl phosphatidic acid (DMPA) and dipalmitoyl phosphatidyl glycerol (DPPG). This was shown with surface pressure isotherms and elasticity and PM-IRRAS measurements in the Langmuir monolayers, in addition to quartz crystal microbalance and fluorescence spectroscopy measurements for Langmuir−Blodgett (LB) films transferred onto solid substrates. Some specificity was noted in the removal action because chitosan was unable to remove BLG incorporated into neutral dipalmitoyl phosphatidyl choline (DPPC) and cholesterol monolayers and had no effect on horseradish peroxidase and urease interacting with DMPA. An obvious biological implication of these findings is to offer reasons that chitosan can remove BLG from lipophilic environments, as reported in the recent literature.</description><identifier>ISSN: 0743-7463</identifier><identifier>EISSN: 1520-5827</identifier><identifier>DOI: 10.1021/la7038762</identifier><identifier>PMID: 18302443</identifier><identifier>CODEN: LANGD5</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Adsorption ; Air ; Chemistry ; Chitosan - chemistry ; Colloidal state and disperse state ; Elasticity ; Exact sciences and technology ; General and physical chemistry ; Kinetics ; Lactoglobulins - chemistry ; Lactoglobulins - isolation & purification ; Langmuir blodgett films ; Membranes, Artificial ; Models, Biological ; Pressure ; Surface physical chemistry ; Surface Properties ; Viscosity ; Water - chemistry</subject><ispartof>Langmuir, 2008-04, Vol.24 (8), p.4150-4156</ispartof><rights>Copyright © 2008 American Chemical Society</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a350t-4fe47464245b60fbf76818c273e795e6c05e048113d65d488a73ff31b8b873d83</citedby><cites>FETCH-LOGICAL-a350t-4fe47464245b60fbf76818c273e795e6c05e048113d65d488a73ff31b8b873d83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/la7038762$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/la7038762$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20239095$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18302443$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Caseli, Luciano</creatorcontrib><creatorcontrib>Pavinatto, Felippe J</creatorcontrib><creatorcontrib>Nobre, Thatyane Morimoto</creatorcontrib><creatorcontrib>Zaniquelli, Maria E. D</creatorcontrib><creatorcontrib>Viitala, Tapani</creatorcontrib><creatorcontrib>Oliveira, Osvaldo N</creatorcontrib><title>Chitosan as a Removing Agent of β-Lactoglobulin from Membrane Models</title><title>Langmuir</title><addtitle>Langmuir</addtitle><description>Many chitosan biological activities depend on the interaction with biomembranes, but so far it has not been possible to obtain molecular-level evidence of chitosan action. In this article, we employ Langmuir phospholipid monolayers as cell membrane models and show that chitosan is able to remove β-lactoglobulin (BLG) from negatively charged dimyristoyl phosphatidic acid (DMPA) and dipalmitoyl phosphatidyl glycerol (DPPG). This was shown with surface pressure isotherms and elasticity and PM-IRRAS measurements in the Langmuir monolayers, in addition to quartz crystal microbalance and fluorescence spectroscopy measurements for Langmuir−Blodgett (LB) films transferred onto solid substrates. Some specificity was noted in the removal action because chitosan was unable to remove BLG incorporated into neutral dipalmitoyl phosphatidyl choline (DPPC) and cholesterol monolayers and had no effect on horseradish peroxidase and urease interacting with DMPA. An obvious biological implication of these findings is to offer reasons that chitosan can remove BLG from lipophilic environments, as reported in the recent literature.</description><subject>Adsorption</subject><subject>Air</subject><subject>Chemistry</subject><subject>Chitosan - chemistry</subject><subject>Colloidal state and disperse state</subject><subject>Elasticity</subject><subject>Exact sciences and technology</subject><subject>General and physical chemistry</subject><subject>Kinetics</subject><subject>Lactoglobulins - chemistry</subject><subject>Lactoglobulins - isolation & purification</subject><subject>Langmuir blodgett films</subject><subject>Membranes, Artificial</subject><subject>Models, Biological</subject><subject>Pressure</subject><subject>Surface physical chemistry</subject><subject>Surface Properties</subject><subject>Viscosity</subject><subject>Water - chemistry</subject><issn>0743-7463</issn><issn>1520-5827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0LtOwzAUBmALgaAUBl4AeWFgCBzfYmdEFTepLQgKA4vlJHYJJDGyUwSvxYPwTAS1KgvTGc6nX-f8CB0QOCFAyWltJDAlU7qBBkRQSISichMNQHKWSJ6yHbQb4wsAZIxn22iHKAaUczZA56PnqvPRtNhEbPCdbfx71c7x2dy2HfYOf38lY1N0fl77fFFXLXbBN3himzyY1uKJL20d99CWM3W0-6s5RA8X57PRVTK-ubwenY0TwwR0CXeW9-dwykWegsudTBVRBZXMykzYtABhgStCWJmKkitlJHOOkVzlSrJSsSE6XuYWwccYrNNvoWpM-NQE9G8Vel1Fbw-X9m2RN7b8k6vfe3C0AiYWpnb9P0UV144CZRlkonfJ0lWxsx_rvQmvOpVMCj27vdcXj1O4ginRT3-5poj6xS9C21fyz4E_rH1_UQ</recordid><startdate>20080415</startdate><enddate>20080415</enddate><creator>Caseli, Luciano</creator><creator>Pavinatto, Felippe J</creator><creator>Nobre, Thatyane Morimoto</creator><creator>Zaniquelli, Maria E. 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D ; Viitala, Tapani ; Oliveira, Osvaldo N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a350t-4fe47464245b60fbf76818c273e795e6c05e048113d65d488a73ff31b8b873d83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adsorption</topic><topic>Air</topic><topic>Chemistry</topic><topic>Chitosan - chemistry</topic><topic>Colloidal state and disperse state</topic><topic>Elasticity</topic><topic>Exact sciences and technology</topic><topic>General and physical chemistry</topic><topic>Kinetics</topic><topic>Lactoglobulins - chemistry</topic><topic>Lactoglobulins - isolation & purification</topic><topic>Langmuir blodgett films</topic><topic>Membranes, Artificial</topic><topic>Models, Biological</topic><topic>Pressure</topic><topic>Surface physical chemistry</topic><topic>Surface Properties</topic><topic>Viscosity</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Caseli, Luciano</creatorcontrib><creatorcontrib>Pavinatto, Felippe J</creatorcontrib><creatorcontrib>Nobre, Thatyane Morimoto</creatorcontrib><creatorcontrib>Zaniquelli, Maria E. D</creatorcontrib><creatorcontrib>Viitala, Tapani</creatorcontrib><creatorcontrib>Oliveira, Osvaldo N</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Langmuir</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Caseli, Luciano</au><au>Pavinatto, Felippe J</au><au>Nobre, Thatyane Morimoto</au><au>Zaniquelli, Maria E. D</au><au>Viitala, Tapani</au><au>Oliveira, Osvaldo N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chitosan as a Removing Agent of β-Lactoglobulin from Membrane Models</atitle><jtitle>Langmuir</jtitle><addtitle>Langmuir</addtitle><date>2008-04-15</date><risdate>2008</risdate><volume>24</volume><issue>8</issue><spage>4150</spage><epage>4156</epage><pages>4150-4156</pages><issn>0743-7463</issn><eissn>1520-5827</eissn><coden>LANGD5</coden><abstract>Many chitosan biological activities depend on the interaction with biomembranes, but so far it has not been possible to obtain molecular-level evidence of chitosan action. In this article, we employ Langmuir phospholipid monolayers as cell membrane models and show that chitosan is able to remove β-lactoglobulin (BLG) from negatively charged dimyristoyl phosphatidic acid (DMPA) and dipalmitoyl phosphatidyl glycerol (DPPG). This was shown with surface pressure isotherms and elasticity and PM-IRRAS measurements in the Langmuir monolayers, in addition to quartz crystal microbalance and fluorescence spectroscopy measurements for Langmuir−Blodgett (LB) films transferred onto solid substrates. Some specificity was noted in the removal action because chitosan was unable to remove BLG incorporated into neutral dipalmitoyl phosphatidyl choline (DPPC) and cholesterol monolayers and had no effect on horseradish peroxidase and urease interacting with DMPA. An obvious biological implication of these findings is to offer reasons that chitosan can remove BLG from lipophilic environments, as reported in the recent literature.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>18302443</pmid><doi>10.1021/la7038762</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Adsorption Air Chemistry Chitosan - chemistry Colloidal state and disperse state Elasticity Exact sciences and technology General and physical chemistry Kinetics Lactoglobulins - chemistry Lactoglobulins - isolation & purification Langmuir blodgett films Membranes, Artificial Models, Biological Pressure Surface physical chemistry Surface Properties Viscosity Water - chemistry |
| title | Chitosan as a Removing Agent of β-Lactoglobulin from Membrane Models |
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