Cellular Compatibility of Biomineralized ZnO Nanoparticles Based on Prokaryotic and Eukaryotic Systems
Zinc oxide nanoparticles (NPs) with the size of ∼100 nm were prepared via a facile biomineralization process in the template of silk fibroin (SF) peptide at room temperature. These ZnO NPs have shown the remarkable behavior of low toxicity to Gram-positive bacteria (Staphylococcus aureus, Staphyloco...
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| Veröffentlicht in: | Langmuir 2011-11, Vol.27 (21), p.13206-13211 |
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| creator | Yan, Danhong Yin, Guangfu Huang, Zhongbing Li, Liang Liao, Xiaoming Chen, Xianchun Yao, Yadong Hao, Baoqing |
| description | Zinc oxide nanoparticles (NPs) with the size of ∼100 nm were prepared via a facile biomineralization process in the template of silk fibroin (SF) peptide at room temperature. These ZnO NPs have shown the remarkable behavior of low toxicity to Gram-positive bacteria (Staphylococcus aureus, Staphylococcus agalactiae), Gram-negative bacteria (Escherichia coli), and eukaryotic cells (mouse L929 fibroblasts). Bacteriological testing indicated that ZnO NPs presented a 50% inhibitory effect on Streptococcus agalactiae at the concentrations of >100 mM, whereas at the same concentrations, the growth of Staphylococcus aureus and Escherichia coli were hardly inhibited. On the other hand, a remarkable proliferation of Staphylococcus aureus or Escherichia coli was observed at the concentrations of ZnO NPs |
| doi_str_mv | 10.1021/la2008107 |
| format | Article |
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These ZnO NPs have shown the remarkable behavior of low toxicity to Gram-positive bacteria (Staphylococcus aureus, Staphylococcus agalactiae), Gram-negative bacteria (Escherichia coli), and eukaryotic cells (mouse L929 fibroblasts). Bacteriological testing indicated that ZnO NPs presented a 50% inhibitory effect on Streptococcus agalactiae at the concentrations of >100 mM, whereas at the same concentrations, the growth of Staphylococcus aureus and Escherichia coli were hardly inhibited. On the other hand, a remarkable proliferation of Staphylococcus aureus or Escherichia coli was observed at the concentrations of ZnO NPs <50 mM. Moreover, the cytotoxicity test demonstrated that ZnO NPs mineralized with SF peptide possessed a low toxicity to mouse L929 fibroblasts. The SF peptide coated on the surface of ZnO NPs permitted greater adhesion and consequently greater proliferation of mouse L929 fibroblasts. Besides, from TEM micrographs of the cell ultrastructure, endocytosis of NPs into the cytoplasm can be detected and the ultrastructure of the cell underwent few changes. The cell membrane retained integrity, euchromatin dispersed homogenously inside the cytoplasm, the mitochondrial architecture remained intact, and no intracellular vacuoles were observed. High-resolution transmission electron microscopy images and selected area electron diffraction patterns of ultrathin cell sections indicated that the crystal structure of NPs was not damaged by the organelle or cytoplasm. All these observations indicated that ZnO NPs mineralized with the SF peptide possess good cytocompatibility.</description><identifier>ISSN: 0743-7463</identifier><identifier>EISSN: 1520-5827</identifier><identifier>DOI: 10.1021/la2008107</identifier><identifier>PMID: 21932858</identifier><identifier>CODEN: LANGD5</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Bacteria - cytology ; Bacteria - drug effects ; Cell Survival ; Chemistry ; Colloidal state and disperse state ; Crystallography, X-Ray ; Exact sciences and technology ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Fibroins - metabolism ; General and physical chemistry ; Materials Testing - methods ; Materials: Nano-and Mesostructured Materials, Polymers, Gels, Liquid Crystals, Composites ; Membranes ; Mice ; Minerals - metabolism ; Nanoparticles - chemistry ; Nanoparticles - toxicity ; Physical and chemical studies. Granulometry. Electrokinetic phenomena ; Temperature ; Water - chemistry ; Zinc Oxide - chemistry ; Zinc Oxide - metabolism ; Zinc Oxide - toxicity</subject><ispartof>Langmuir, 2011-11, Vol.27 (21), p.13206-13211</ispartof><rights>Copyright © 2011 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a344t-24b8f0a9e612eada3ffce4b4751d6c2c42ad3fb6ec4ecf623cc22d50029671f23</citedby><cites>FETCH-LOGICAL-a344t-24b8f0a9e612eada3ffce4b4751d6c2c42ad3fb6ec4ecf623cc22d50029671f23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/la2008107$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/la2008107$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24704508$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21932858$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yan, Danhong</creatorcontrib><creatorcontrib>Yin, Guangfu</creatorcontrib><creatorcontrib>Huang, Zhongbing</creatorcontrib><creatorcontrib>Li, Liang</creatorcontrib><creatorcontrib>Liao, Xiaoming</creatorcontrib><creatorcontrib>Chen, Xianchun</creatorcontrib><creatorcontrib>Yao, Yadong</creatorcontrib><creatorcontrib>Hao, Baoqing</creatorcontrib><title>Cellular Compatibility of Biomineralized ZnO Nanoparticles Based on Prokaryotic and Eukaryotic Systems</title><title>Langmuir</title><addtitle>Langmuir</addtitle><description>Zinc oxide nanoparticles (NPs) with the size of ∼100 nm were prepared via a facile biomineralization process in the template of silk fibroin (SF) peptide at room temperature. These ZnO NPs have shown the remarkable behavior of low toxicity to Gram-positive bacteria (Staphylococcus aureus, Staphylococcus agalactiae), Gram-negative bacteria (Escherichia coli), and eukaryotic cells (mouse L929 fibroblasts). Bacteriological testing indicated that ZnO NPs presented a 50% inhibitory effect on Streptococcus agalactiae at the concentrations of >100 mM, whereas at the same concentrations, the growth of Staphylococcus aureus and Escherichia coli were hardly inhibited. On the other hand, a remarkable proliferation of Staphylococcus aureus or Escherichia coli was observed at the concentrations of ZnO NPs <50 mM. Moreover, the cytotoxicity test demonstrated that ZnO NPs mineralized with SF peptide possessed a low toxicity to mouse L929 fibroblasts. The SF peptide coated on the surface of ZnO NPs permitted greater adhesion and consequently greater proliferation of mouse L929 fibroblasts. Besides, from TEM micrographs of the cell ultrastructure, endocytosis of NPs into the cytoplasm can be detected and the ultrastructure of the cell underwent few changes. The cell membrane retained integrity, euchromatin dispersed homogenously inside the cytoplasm, the mitochondrial architecture remained intact, and no intracellular vacuoles were observed. High-resolution transmission electron microscopy images and selected area electron diffraction patterns of ultrathin cell sections indicated that the crystal structure of NPs was not damaged by the organelle or cytoplasm. All these observations indicated that ZnO NPs mineralized with the SF peptide possess good cytocompatibility.</description><subject>Animals</subject><subject>Bacteria - cytology</subject><subject>Bacteria - drug effects</subject><subject>Cell Survival</subject><subject>Chemistry</subject><subject>Colloidal state and disperse state</subject><subject>Crystallography, X-Ray</subject><subject>Exact sciences and technology</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroins - metabolism</subject><subject>General and physical chemistry</subject><subject>Materials Testing - methods</subject><subject>Materials: Nano-and Mesostructured Materials, Polymers, Gels, Liquid Crystals, Composites</subject><subject>Membranes</subject><subject>Mice</subject><subject>Minerals - metabolism</subject><subject>Nanoparticles - chemistry</subject><subject>Nanoparticles - toxicity</subject><subject>Physical and chemical studies. Granulometry. Electrokinetic phenomena</subject><subject>Temperature</subject><subject>Water - chemistry</subject><subject>Zinc Oxide - chemistry</subject><subject>Zinc Oxide - metabolism</subject><subject>Zinc Oxide - toxicity</subject><issn>0743-7463</issn><issn>1520-5827</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkLtOwzAUhi0EoqUw8ALICwNDwLfcRhqVi1RRJGBhiU4cW3Jx4shOhvL0BLUUBqaj8-vTuXwInVNyTQmjNxYYIRkl6QGa0piRKM5YeoimJBU8SkXCJ-gkhDUhJOciP0YTRnPOsjibIl0oawcLHheu6aA3lbGm32Cn8dy4xrTKgzWfqsbv7Qo_Qes68L2RVgU8hzDmrsXP3n2A37gxx9DWeDHs25dN6FUTTtGRBhvU2a7O0Nvd4rV4iJar-8fidhkBF6KPmKgyTSBXCWUKauBaSyUqkca0TiSTgkHNdZUoKZTUCeNSMlbHhLA8SalmfIautnOldyF4pcvOm2Y8pqSk_HZV7l2N7MWW7YaqUfWe_JEzApc7AIIEqz200oRfTqRExOQPBzKUazf4dnzxn4VfJZt-lw</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Yan, Danhong</creator><creator>Yin, Guangfu</creator><creator>Huang, Zhongbing</creator><creator>Li, Liang</creator><creator>Liao, Xiaoming</creator><creator>Chen, Xianchun</creator><creator>Yao, Yadong</creator><creator>Hao, Baoqing</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20111101</creationdate><title>Cellular Compatibility of Biomineralized ZnO Nanoparticles Based on Prokaryotic and Eukaryotic Systems</title><author>Yan, Danhong ; Yin, Guangfu ; Huang, Zhongbing ; Li, Liang ; Liao, Xiaoming ; Chen, Xianchun ; Yao, Yadong ; Hao, Baoqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a344t-24b8f0a9e612eada3ffce4b4751d6c2c42ad3fb6ec4ecf623cc22d50029671f23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Bacteria - cytology</topic><topic>Bacteria - drug effects</topic><topic>Cell Survival</topic><topic>Chemistry</topic><topic>Colloidal state and disperse state</topic><topic>Crystallography, X-Ray</topic><topic>Exact sciences and technology</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroins - metabolism</topic><topic>General and physical chemistry</topic><topic>Materials Testing - methods</topic><topic>Materials: Nano-and Mesostructured Materials, Polymers, Gels, Liquid Crystals, Composites</topic><topic>Membranes</topic><topic>Mice</topic><topic>Minerals - metabolism</topic><topic>Nanoparticles - chemistry</topic><topic>Nanoparticles - toxicity</topic><topic>Physical and chemical studies. Granulometry. Electrokinetic phenomena</topic><topic>Temperature</topic><topic>Water - chemistry</topic><topic>Zinc Oxide - chemistry</topic><topic>Zinc Oxide - metabolism</topic><topic>Zinc Oxide - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yan, Danhong</creatorcontrib><creatorcontrib>Yin, Guangfu</creatorcontrib><creatorcontrib>Huang, Zhongbing</creatorcontrib><creatorcontrib>Li, Liang</creatorcontrib><creatorcontrib>Liao, Xiaoming</creatorcontrib><creatorcontrib>Chen, Xianchun</creatorcontrib><creatorcontrib>Yao, Yadong</creatorcontrib><creatorcontrib>Hao, Baoqing</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Langmuir</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yan, Danhong</au><au>Yin, Guangfu</au><au>Huang, Zhongbing</au><au>Li, Liang</au><au>Liao, Xiaoming</au><au>Chen, Xianchun</au><au>Yao, Yadong</au><au>Hao, Baoqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cellular Compatibility of Biomineralized ZnO Nanoparticles Based on Prokaryotic and Eukaryotic Systems</atitle><jtitle>Langmuir</jtitle><addtitle>Langmuir</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>27</volume><issue>21</issue><spage>13206</spage><epage>13211</epage><pages>13206-13211</pages><issn>0743-7463</issn><eissn>1520-5827</eissn><coden>LANGD5</coden><abstract>Zinc oxide nanoparticles (NPs) with the size of ∼100 nm were prepared via a facile biomineralization process in the template of silk fibroin (SF) peptide at room temperature. These ZnO NPs have shown the remarkable behavior of low toxicity to Gram-positive bacteria (Staphylococcus aureus, Staphylococcus agalactiae), Gram-negative bacteria (Escherichia coli), and eukaryotic cells (mouse L929 fibroblasts). Bacteriological testing indicated that ZnO NPs presented a 50% inhibitory effect on Streptococcus agalactiae at the concentrations of >100 mM, whereas at the same concentrations, the growth of Staphylococcus aureus and Escherichia coli were hardly inhibited. On the other hand, a remarkable proliferation of Staphylococcus aureus or Escherichia coli was observed at the concentrations of ZnO NPs <50 mM. Moreover, the cytotoxicity test demonstrated that ZnO NPs mineralized with SF peptide possessed a low toxicity to mouse L929 fibroblasts. The SF peptide coated on the surface of ZnO NPs permitted greater adhesion and consequently greater proliferation of mouse L929 fibroblasts. Besides, from TEM micrographs of the cell ultrastructure, endocytosis of NPs into the cytoplasm can be detected and the ultrastructure of the cell underwent few changes. The cell membrane retained integrity, euchromatin dispersed homogenously inside the cytoplasm, the mitochondrial architecture remained intact, and no intracellular vacuoles were observed. High-resolution transmission electron microscopy images and selected area electron diffraction patterns of ultrathin cell sections indicated that the crystal structure of NPs was not damaged by the organelle or cytoplasm. All these observations indicated that ZnO NPs mineralized with the SF peptide possess good cytocompatibility.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>21932858</pmid><doi>10.1021/la2008107</doi><tpages>6</tpages></addata></record> |
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| subjects | Animals Bacteria - cytology Bacteria - drug effects Cell Survival Chemistry Colloidal state and disperse state Crystallography, X-Ray Exact sciences and technology Fibroblasts - cytology Fibroblasts - drug effects Fibroins - metabolism General and physical chemistry Materials Testing - methods Materials: Nano-and Mesostructured Materials, Polymers, Gels, Liquid Crystals, Composites Membranes Mice Minerals - metabolism Nanoparticles - chemistry Nanoparticles - toxicity Physical and chemical studies. Granulometry. Electrokinetic phenomena Temperature Water - chemistry Zinc Oxide - chemistry Zinc Oxide - metabolism Zinc Oxide - toxicity |
| title | Cellular Compatibility of Biomineralized ZnO Nanoparticles Based on Prokaryotic and Eukaryotic Systems |
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