Folding of Deoxymyoglobin Triggered by Electron Transfer

Folding of Deoxymyoglobin Triggered by Electron Transfer

The met and deoxy forms of sperm whale myoglobin (Mb) can be unfolded by guanidine hydrochloride (GuHCl). Electronic absorption and circular dichroism spectroscopic measurements show that folded deoxyMb is more stable than the folded met protein. Laser excitation of NADH generates species that rapid...

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Veröffentlicht in:The journal of physical chemistry. A, Molecules, spectroscopy, kinetics, environment, & general theory Molecules, spectroscopy, kinetics, environment, & general theory, 1998-07, Vol.102 (28), p.5599-5601
Hauptverfasser: Wittung-Stafshede, Pernilla, Malmström, Bo G, Winkler, Jay R, Gray, Harry B
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container_issue 28
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container_title The journal of physical chemistry. A, Molecules, spectroscopy, kinetics, environment, & general theory
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creator Wittung-Stafshede, Pernilla
Malmström, Bo G
Winkler, Jay R
Gray, Harry B
description The met and deoxy forms of sperm whale myoglobin (Mb) can be unfolded by guanidine hydrochloride (GuHCl). Electronic absorption and circular dichroism spectroscopic measurements show that folded deoxyMb is more stable than the folded met protein. Laser excitation of NADH generates species that rapidly reduce unfolded metMb, triggering the formation of folded deoxyMb in less than 10 ms (pH 7.0, 2.5 to 3 M GuHCl, 20 °C). At comparable reaction driving forces (∼10 kJ/mol), deoxyMb folds much faster than reduced cytochrome c.
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title Folding of Deoxymyoglobin Triggered by Electron Transfer
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