Probing Cytotoxicity of Gadolinium Hydroxide Nanostructures
Gadolinium hydroxide, Gd(OH)3, nanostructures were examined for their possible use in imaging and tracking of cells and tissues by investigating their cellular interactions and cytotoxic behaviors. For this purpose, Gd(OH)3 nanorods (length, several hundred nanometers; diameter, ∼40 nm) and spherica...
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| Veröffentlicht in: | The journal of physical chemistry. B 2010-04, Vol.114 (12), p.4358-4365 |
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| container_issue | 12 |
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| container_title | The journal of physical chemistry. B |
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| creator | Hemmer, Eva Kohl, Yvonne Colquhoun, Victoria Thielecke, Hagen Soga, Kohei Mathur, Sanjay |
| description | Gadolinium hydroxide, Gd(OH)3, nanostructures were examined for their possible use in imaging and tracking of cells and tissues by investigating their cellular interactions and cytotoxic behaviors. For this purpose, Gd(OH)3 nanorods (length, several hundred nanometers; diameter, ∼40 nm) and spherical nanoparticles (average diameter, |
| doi_str_mv | 10.1021/jp911607h |
| format | Article |
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For this purpose, Gd(OH)3 nanorods (length, several hundred nanometers; diameter, ∼40 nm) and spherical nanoparticles (average diameter, <10 nm) were synthesized by solvothermal decomposition of gadolinium containing molecular precursors. After comprehensive characterization of material properties, human colon adenocarcinoma (Caco2) and human lung epithelial (A549) cells were incubated with Gd(OH)3 nanostructures in concentrations up to 900 μg/mL to perform cytotoxicity assays (BrdU (5-bromo-2′-deoxyuridine), WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzol-disulfonate)) and living/dead staining. As observed in all in vitro assays, the investigated Gd(OH)3 nanostructures do not induce any significant cytotoxic effect, making them promising candidates for a new class of contrast agents, which may overcome the limitations of organic stains such as photobleaching and single usage.</description><identifier>ISSN: 1520-6106</identifier><identifier>EISSN: 1520-5207</identifier><identifier>DOI: 10.1021/jp911607h</identifier><identifier>PMID: 20218658</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>B: Biophysical Chemistry ; Cell Line, Tumor ; Cell Survival - drug effects ; Gadolinium - chemistry ; Gadolinium - toxicity ; Humans ; Nanostructures</subject><ispartof>The journal of physical chemistry. 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As observed in all in vitro assays, the investigated Gd(OH)3 nanostructures do not induce any significant cytotoxic effect, making them promising candidates for a new class of contrast agents, which may overcome the limitations of organic stains such as photobleaching and single usage.</description><subject>B: Biophysical Chemistry</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Gadolinium - chemistry</subject><subject>Gadolinium - toxicity</subject><subject>Humans</subject><subject>Nanostructures</subject><issn>1520-6106</issn><issn>1520-5207</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE9LxDAQxYMo7rp68AtILx48VCdpk6Z4kqK7wqIe9Fzyr9qybUqSgv32VrruycMwA_Obx5uH0CWGWwwE3zV9jjGD7OsILTElEE-VHe9nhoEt0Jn3DQChhLNTtCDTFWeUL9H9m7Oy7j6jYgw22O9a1WGMbBWthba7uquHNtqM2k0bbaIX0Vkf3KDC4Iw_RyeV2Hlzse8r9PH0-F5s4u3r-rl42MYi4RBiw6k0qai0YFRlFcklkxnnlOgUVwAykZyAokmqDaSZMFLnXOZMsYTRJOMqWaGbWVc5670zVdm7uhVuLDGUvwGUhwAm9mpm-0G2Rh_Iv48n4HoGhPJlYwfXTdb_EfoBep1ibg</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Hemmer, Eva</creator><creator>Kohl, Yvonne</creator><creator>Colquhoun, Victoria</creator><creator>Thielecke, Hagen</creator><creator>Soga, Kohei</creator><creator>Mathur, Sanjay</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20100401</creationdate><title>Probing Cytotoxicity of Gadolinium Hydroxide Nanostructures</title><author>Hemmer, Eva ; Kohl, Yvonne ; Colquhoun, Victoria ; Thielecke, Hagen ; Soga, Kohei ; Mathur, Sanjay</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a380t-e85be4afda65c7f29b6b78852d41f00b3b820c534de047aebd98b96c6365378c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>B: Biophysical Chemistry</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Gadolinium - chemistry</topic><topic>Gadolinium - toxicity</topic><topic>Humans</topic><topic>Nanostructures</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hemmer, Eva</creatorcontrib><creatorcontrib>Kohl, Yvonne</creatorcontrib><creatorcontrib>Colquhoun, Victoria</creatorcontrib><creatorcontrib>Thielecke, Hagen</creatorcontrib><creatorcontrib>Soga, Kohei</creatorcontrib><creatorcontrib>Mathur, Sanjay</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>The journal of physical chemistry. B</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hemmer, Eva</au><au>Kohl, Yvonne</au><au>Colquhoun, Victoria</au><au>Thielecke, Hagen</au><au>Soga, Kohei</au><au>Mathur, Sanjay</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Probing Cytotoxicity of Gadolinium Hydroxide Nanostructures</atitle><jtitle>The journal of physical chemistry. B</jtitle><addtitle>J. Phys. Chem. B</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>114</volume><issue>12</issue><spage>4358</spage><epage>4365</epage><pages>4358-4365</pages><issn>1520-6106</issn><eissn>1520-5207</eissn><abstract>Gadolinium hydroxide, Gd(OH)3, nanostructures were examined for their possible use in imaging and tracking of cells and tissues by investigating their cellular interactions and cytotoxic behaviors. For this purpose, Gd(OH)3 nanorods (length, several hundred nanometers; diameter, ∼40 nm) and spherical nanoparticles (average diameter, <10 nm) were synthesized by solvothermal decomposition of gadolinium containing molecular precursors. After comprehensive characterization of material properties, human colon adenocarcinoma (Caco2) and human lung epithelial (A549) cells were incubated with Gd(OH)3 nanostructures in concentrations up to 900 μg/mL to perform cytotoxicity assays (BrdU (5-bromo-2′-deoxyuridine), WST-1 (4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1,3-benzol-disulfonate)) and living/dead staining. As observed in all in vitro assays, the investigated Gd(OH)3 nanostructures do not induce any significant cytotoxic effect, making them promising candidates for a new class of contrast agents, which may overcome the limitations of organic stains such as photobleaching and single usage.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>20218658</pmid><doi>10.1021/jp911607h</doi><tpages>8</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | B: Biophysical Chemistry Cell Line, Tumor Cell Survival - drug effects Gadolinium - chemistry Gadolinium - toxicity Humans Nanostructures |
| title | Probing Cytotoxicity of Gadolinium Hydroxide Nanostructures |
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