Development of a Compound-Specific ELISA for Flufenoxuron and an Improved Class-Specific Assay for Benzoylphenylurea Insect Growth Regulators
This study describes immunochemical approaches for the compound-specific detection of flufenoxuron and class-specific detection of benzoylphenylurea (BPU) insecticides. With the aim of developing a highly specific immunoassay for flufenoxuron, a hapten was synthesized by introducing a spacer arm at...
Gespeichert in:
| Veröffentlicht in: | Journal of agricultural and food chemistry 1999-08, Vol.47 (8), p.3416-3424 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3424 |
|---|---|
| container_issue | 8 |
| container_start_page | 3416 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 47 |
| creator | Wang, Shuo Allan, Robin D Skerritt, John H Kennedy, Ivan R |
| description | This study describes immunochemical approaches for the compound-specific detection of flufenoxuron and class-specific detection of benzoylphenylurea (BPU) insecticides. With the aim of developing a highly specific immunoassay for flufenoxuron, a hapten was synthesized by introducing a spacer arm at the 2,6-difluoro substituent aromatic ring of a flufenoxuron derivative. An IC50 value of 2.4 ppb was obtained for flufenoxuron, with detection of the other four BPUs being more than 4000-fold less sensitive. For the development of class-specific ELISA for five BPUs, a new approach was used for the hapten preparation in which a butanoic acid linkage was introduced into the 3,5-dichloro-substitued aniline ring of chlorfluazuron analogue. Although the resultant ELISA still exhibited slightly differing cross-reactions for these five BPUs, this method had broader specificity than the previously reported polyclonal antibody-based ELISA. Spike and recovery studies for five BPUs in soil and water indicated that both the compound- and class-specific ELISAs were able to quantitatively detect BPU residues in soil and water. This study also provided additional insights into the influence of the immunizing hapten structure on the specificities of the antibodies obtained. Keywords: Flufenoxuron; benzoylphenylurea, compound-specific detection; class-specific detection, ELISA |
| doi_str_mv | 10.1021/jf9812984 |
| format | Article |
| fullrecord | <record><control><sourceid>istex_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1021_jf9812984</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>ark_67375_TPS_T9PQ59Z8_5</sourcerecordid><originalsourceid>FETCH-LOGICAL-a402t-38c6a6bbbfac89382b7ba5a6d5b1afce83e8dfb284a29b3d7fb84a42a9f4a0e33</originalsourceid><addsrcrecordid>eNpt0M1u1DAQB3ALgehSOPAC4AMcOAT8ESfOcbu0ZaWVKOz2wsUaO3abJWtHdtJ2-w68M2lTtRw4jDzS_GYk_xF6S8lnShj9snWVpKyS-TM0o4KRTFAqn6MZGYeZFAU9QK9S2hJCpCjJS3RAiRCsKIoZ-vPVXtk2dDvrexwcBrwIuy4Mvs7WnTWNaww-Xi3Xc-xCxCft4KwPN0MMHoOvx8LLXRfDla3xooWUnrbmKcH-fuvI-tuwb7tL6_ftEC3gpU_W9Pg0huv-Ev-0F0MLfYjpNXrhoE32zcN7iM5PjjeLb9nq--lyMV9lkBPWZ1yaAgqttQMjKy6ZLjUIKGqhKThjJbeydprJHFileV06PbY5g8rlQCznh-jTdNfEkFK0TnWx2UHcK0rUXaTqMdLRvptsN-idrf-RU4Yj-PAAIBloXQRvmvTkGOf5Pcsm1qTe3jyOIf5WRclLoTZna7Wpzn6I6pdUYvTvJ-8gKLiI48nzNSOUE1blUog78XESYJLahiH6MbP__OAv2x2lDQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Development of a Compound-Specific ELISA for Flufenoxuron and an Improved Class-Specific Assay for Benzoylphenylurea Insect Growth Regulators</title><source>MEDLINE</source><source>ACS Publications</source><creator>Wang, Shuo ; Allan, Robin D ; Skerritt, John H ; Kennedy, Ivan R</creator><creatorcontrib>Wang, Shuo ; Allan, Robin D ; Skerritt, John H ; Kennedy, Ivan R</creatorcontrib><description>This study describes immunochemical approaches for the compound-specific detection of flufenoxuron and class-specific detection of benzoylphenylurea (BPU) insecticides. With the aim of developing a highly specific immunoassay for flufenoxuron, a hapten was synthesized by introducing a spacer arm at the 2,6-difluoro substituent aromatic ring of a flufenoxuron derivative. An IC50 value of 2.4 ppb was obtained for flufenoxuron, with detection of the other four BPUs being more than 4000-fold less sensitive. For the development of class-specific ELISA for five BPUs, a new approach was used for the hapten preparation in which a butanoic acid linkage was introduced into the 3,5-dichloro-substitued aniline ring of chlorfluazuron analogue. Although the resultant ELISA still exhibited slightly differing cross-reactions for these five BPUs, this method had broader specificity than the previously reported polyclonal antibody-based ELISA. Spike and recovery studies for five BPUs in soil and water indicated that both the compound- and class-specific ELISAs were able to quantitatively detect BPU residues in soil and water. This study also provided additional insights into the influence of the immunizing hapten structure on the specificities of the antibodies obtained. Keywords: Flufenoxuron; benzoylphenylurea, compound-specific detection; class-specific detection, ELISA</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf9812984</identifier><identifier>PMID: 10552666</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Antibody Specificity ; benzoylphenylureas ; Biological and medical sciences ; Chemical control ; class-specific assays ; compound-selective assays ; Control ; Cross Reactions ; detection ; enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; flufenoxuron ; Fundamental and applied biological sciences. Psychology ; insect growth regulators ; Insecticides - analysis ; Insecticides - immunology ; Molecular Structure ; Phenylurea Compounds - analysis ; Phenylurea Compounds - chemistry ; Phenylurea Compounds - immunology ; Phytopathology. Animal pests. Plant and forest protection ; Protozoa. Invertebrates ; Rabbits ; Sensitivity and Specificity ; Soil - analysis ; Water - analysis</subject><ispartof>Journal of agricultural and food chemistry, 1999-08, Vol.47 (8), p.3416-3424</ispartof><rights>Copyright © 1999 American Chemical Society</rights><rights>2000 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a402t-38c6a6bbbfac89382b7ba5a6d5b1afce83e8dfb284a29b3d7fb84a42a9f4a0e33</citedby><cites>FETCH-LOGICAL-a402t-38c6a6bbbfac89382b7ba5a6d5b1afce83e8dfb284a29b3d7fb84a42a9f4a0e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf9812984$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf9812984$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1233466$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10552666$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Shuo</creatorcontrib><creatorcontrib>Allan, Robin D</creatorcontrib><creatorcontrib>Skerritt, John H</creatorcontrib><creatorcontrib>Kennedy, Ivan R</creatorcontrib><title>Development of a Compound-Specific ELISA for Flufenoxuron and an Improved Class-Specific Assay for Benzoylphenylurea Insect Growth Regulators</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>This study describes immunochemical approaches for the compound-specific detection of flufenoxuron and class-specific detection of benzoylphenylurea (BPU) insecticides. With the aim of developing a highly specific immunoassay for flufenoxuron, a hapten was synthesized by introducing a spacer arm at the 2,6-difluoro substituent aromatic ring of a flufenoxuron derivative. An IC50 value of 2.4 ppb was obtained for flufenoxuron, with detection of the other four BPUs being more than 4000-fold less sensitive. For the development of class-specific ELISA for five BPUs, a new approach was used for the hapten preparation in which a butanoic acid linkage was introduced into the 3,5-dichloro-substitued aniline ring of chlorfluazuron analogue. Although the resultant ELISA still exhibited slightly differing cross-reactions for these five BPUs, this method had broader specificity than the previously reported polyclonal antibody-based ELISA. Spike and recovery studies for five BPUs in soil and water indicated that both the compound- and class-specific ELISAs were able to quantitatively detect BPU residues in soil and water. This study also provided additional insights into the influence of the immunizing hapten structure on the specificities of the antibodies obtained. Keywords: Flufenoxuron; benzoylphenylurea, compound-specific detection; class-specific detection, ELISA</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>benzoylphenylureas</subject><subject>Biological and medical sciences</subject><subject>Chemical control</subject><subject>class-specific assays</subject><subject>compound-selective assays</subject><subject>Control</subject><subject>Cross Reactions</subject><subject>detection</subject><subject>enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>flufenoxuron</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>insect growth regulators</subject><subject>Insecticides - analysis</subject><subject>Insecticides - immunology</subject><subject>Molecular Structure</subject><subject>Phenylurea Compounds - analysis</subject><subject>Phenylurea Compounds - chemistry</subject><subject>Phenylurea Compounds - immunology</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Protozoa. Invertebrates</subject><subject>Rabbits</subject><subject>Sensitivity and Specificity</subject><subject>Soil - analysis</subject><subject>Water - analysis</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0M1u1DAQB3ALgehSOPAC4AMcOAT8ESfOcbu0ZaWVKOz2wsUaO3abJWtHdtJ2-w68M2lTtRw4jDzS_GYk_xF6S8lnShj9snWVpKyS-TM0o4KRTFAqn6MZGYeZFAU9QK9S2hJCpCjJS3RAiRCsKIoZ-vPVXtk2dDvrexwcBrwIuy4Mvs7WnTWNaww-Xi3Xc-xCxCft4KwPN0MMHoOvx8LLXRfDla3xooWUnrbmKcH-fuvI-tuwb7tL6_ftEC3gpU_W9Pg0huv-Ev-0F0MLfYjpNXrhoE32zcN7iM5PjjeLb9nq--lyMV9lkBPWZ1yaAgqttQMjKy6ZLjUIKGqhKThjJbeydprJHFileV06PbY5g8rlQCznh-jTdNfEkFK0TnWx2UHcK0rUXaTqMdLRvptsN-idrf-RU4Yj-PAAIBloXQRvmvTkGOf5Pcsm1qTe3jyOIf5WRclLoTZna7Wpzn6I6pdUYvTvJ-8gKLiI48nzNSOUE1blUog78XESYJLahiH6MbP__OAv2x2lDQ</recordid><startdate>19990801</startdate><enddate>19990801</enddate><creator>Wang, Shuo</creator><creator>Allan, Robin D</creator><creator>Skerritt, John H</creator><creator>Kennedy, Ivan R</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19990801</creationdate><title>Development of a Compound-Specific ELISA for Flufenoxuron and an Improved Class-Specific Assay for Benzoylphenylurea Insect Growth Regulators</title><author>Wang, Shuo ; Allan, Robin D ; Skerritt, John H ; Kennedy, Ivan R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a402t-38c6a6bbbfac89382b7ba5a6d5b1afce83e8dfb284a29b3d7fb84a42a9f4a0e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>benzoylphenylureas</topic><topic>Biological and medical sciences</topic><topic>Chemical control</topic><topic>class-specific assays</topic><topic>compound-selective assays</topic><topic>Control</topic><topic>Cross Reactions</topic><topic>detection</topic><topic>enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>flufenoxuron</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>insect growth regulators</topic><topic>Insecticides - analysis</topic><topic>Insecticides - immunology</topic><topic>Molecular Structure</topic><topic>Phenylurea Compounds - analysis</topic><topic>Phenylurea Compounds - chemistry</topic><topic>Phenylurea Compounds - immunology</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Protozoa. Invertebrates</topic><topic>Rabbits</topic><topic>Sensitivity and Specificity</topic><topic>Soil - analysis</topic><topic>Water - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Shuo</creatorcontrib><creatorcontrib>Allan, Robin D</creatorcontrib><creatorcontrib>Skerritt, John H</creatorcontrib><creatorcontrib>Kennedy, Ivan R</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Shuo</au><au>Allan, Robin D</au><au>Skerritt, John H</au><au>Kennedy, Ivan R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a Compound-Specific ELISA for Flufenoxuron and an Improved Class-Specific Assay for Benzoylphenylurea Insect Growth Regulators</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>1999-08-01</date><risdate>1999</risdate><volume>47</volume><issue>8</issue><spage>3416</spage><epage>3424</epage><pages>3416-3424</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>This study describes immunochemical approaches for the compound-specific detection of flufenoxuron and class-specific detection of benzoylphenylurea (BPU) insecticides. With the aim of developing a highly specific immunoassay for flufenoxuron, a hapten was synthesized by introducing a spacer arm at the 2,6-difluoro substituent aromatic ring of a flufenoxuron derivative. An IC50 value of 2.4 ppb was obtained for flufenoxuron, with detection of the other four BPUs being more than 4000-fold less sensitive. For the development of class-specific ELISA for five BPUs, a new approach was used for the hapten preparation in which a butanoic acid linkage was introduced into the 3,5-dichloro-substitued aniline ring of chlorfluazuron analogue. Although the resultant ELISA still exhibited slightly differing cross-reactions for these five BPUs, this method had broader specificity than the previously reported polyclonal antibody-based ELISA. Spike and recovery studies for five BPUs in soil and water indicated that both the compound- and class-specific ELISAs were able to quantitatively detect BPU residues in soil and water. This study also provided additional insights into the influence of the immunizing hapten structure on the specificities of the antibodies obtained. Keywords: Flufenoxuron; benzoylphenylurea, compound-specific detection; class-specific detection, ELISA</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>10552666</pmid><doi>10.1021/jf9812984</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 1999-08, Vol.47 (8), p.3416-3424 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_crossref_primary_10_1021_jf9812984 |
| source | MEDLINE; ACS Publications |
| subjects | Animals Antibody Specificity benzoylphenylureas Biological and medical sciences Chemical control class-specific assays compound-selective assays Control Cross Reactions detection enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Female flufenoxuron Fundamental and applied biological sciences. Psychology insect growth regulators Insecticides - analysis Insecticides - immunology Molecular Structure Phenylurea Compounds - analysis Phenylurea Compounds - chemistry Phenylurea Compounds - immunology Phytopathology. Animal pests. Plant and forest protection Protozoa. Invertebrates Rabbits Sensitivity and Specificity Soil - analysis Water - analysis |
| title | Development of a Compound-Specific ELISA for Flufenoxuron and an Improved Class-Specific Assay for Benzoylphenylurea Insect Growth Regulators |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T07%3A55%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-istex_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20Compound-Specific%20ELISA%20for%20Flufenoxuron%20and%20an%20Improved%20Class-Specific%20Assay%20for%20Benzoylphenylurea%20Insect%20Growth%20Regulators&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Wang,%20Shuo&rft.date=1999-08-01&rft.volume=47&rft.issue=8&rft.spage=3416&rft.epage=3424&rft.pages=3416-3424&rft.issn=0021-8561&rft.eissn=1520-5118&rft.coden=JAFCAU&rft_id=info:doi/10.1021/jf9812984&rft_dat=%3Cistex_cross%3Eark_67375_TPS_T9PQ59Z8_5%3C/istex_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/10552666&rfr_iscdi=true |