Saccharification of Pretreated Oil Palm Empty Fruit Bunch Fiber Using Cellulase of Chaetomium globosum
The effectiveness of different chemical and physical pretreatments to alter cellulose structure and to reduce hemicellullose and lignin contents in oil palm empty fruit bunch (OPEFB) fibers for subsequent use as substrate for enzymatic saccharification was studied. The saccharification was carried o...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 1998-08, Vol.46 (8), p.3359-3364 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Umikalsom, Md S Ariff, Arbakariya B Karim, Mohamed I. A |
| description | The effectiveness of different chemical and physical pretreatments to alter cellulose structure and to reduce hemicellullose and lignin contents in oil palm empty fruit bunch (OPEFB) fibers for subsequent use as substrate for enzymatic saccharification was studied. The saccharification was carried out using concentrated cellulase preparation from culture broth of Chaetomium globosum strain 414 containing 10 U/mL FPase, 285 U/mL CMCase, and 60 U/mL β-glucosidase. The use of 0.5% NaOH to treat OPEFB by soaking at 30 °C for 4 h gave the highest rate and degree of hydrolysis followed by 0.5% HNO3, HCl, EDA, and EDTA. Autoclaving the chemically treated OPEFB fiber at 121 °C for 5 min improved the hydrolysis by 2-fold. The improvement in hydrolysis was related to a decrease in the hemicellulose and lignin contents and an increase in the cellulose content. The qualitative hydrolysis yield for autoclaved OPEFB fiber treated with 2% NaOH was 85.9%. During saccharification of OPEFB using cellulase of C. globosum, the amount of glucose produced was higher while the amount of cellobiose produced was lower than those obtained with commercial cellulase of Trichoderma viride. Keywords: Cellulase; endoglucanase; cellobiohydrolase; β-glucosidase; Chaetomium globosum; cellulosic material; oil palm empty fruit bunch |
| doi_str_mv | 10.1021/jf971098c |
| format | Article |
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A</creator><creatorcontrib>Umikalsom, Md S ; Ariff, Arbakariya B ; Karim, Mohamed I. A</creatorcontrib><description>The effectiveness of different chemical and physical pretreatments to alter cellulose structure and to reduce hemicellullose and lignin contents in oil palm empty fruit bunch (OPEFB) fibers for subsequent use as substrate for enzymatic saccharification was studied. The saccharification was carried out using concentrated cellulase preparation from culture broth of Chaetomium globosum strain 414 containing 10 U/mL FPase, 285 U/mL CMCase, and 60 U/mL β-glucosidase. The use of 0.5% NaOH to treat OPEFB by soaking at 30 °C for 4 h gave the highest rate and degree of hydrolysis followed by 0.5% HNO3, HCl, EDA, and EDTA. Autoclaving the chemically treated OPEFB fiber at 121 °C for 5 min improved the hydrolysis by 2-fold. The improvement in hydrolysis was related to a decrease in the hemicellulose and lignin contents and an increase in the cellulose content. The qualitative hydrolysis yield for autoclaved OPEFB fiber treated with 2% NaOH was 85.9%. During saccharification of OPEFB using cellulase of C. globosum, the amount of glucose produced was higher while the amount of cellobiose produced was lower than those obtained with commercial cellulase of Trichoderma viride. Keywords: Cellulase; endoglucanase; cellobiohydrolase; β-glucosidase; Chaetomium globosum; cellulosic material; oil palm empty fruit bunch</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf971098c</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>ALKALI TREATMENT ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; CELLULASE ; CELLULOSE ; CELULASA ; CELULOSA ; CHAETOMIUM GLOBOSUM ; DECHET ; DESECHOS ; DIETARY FIBRES ; ENZYMATIC SACCHARIFICATION ; ESTERILIZACION TERMICA ; FIBRA DE LA DIETA ; FIBRE ALIMENTAIRE ; Food industries ; Fundamental and applied biological sciences. Psychology ; HEAT STERILIZING ; HEMICELLULOSE ; HEMICELULOSA ; HIDROLISIS ; HYDROLYSE ; HYDROLYSIS ; LIGNINAS ; LIGNINE ; LIGNINS ; LIGNOCELLULOSE ; LIGNOCELULOSA ; Methods. Procedures. Technologies ; OIL PALMS ; PALMAS OLEAGINOSAS ; PALMIER OLEIFERE ; PRETREATMENT ; PRODUCTIVIDAD ; PRODUCTIVITE ; PRODUCTIVITY ; RENDEMENT ; RENDIMIENTO ; STERILISATION THERMIQUE ; TRAITEMENT AUX ALCALIS ; TRATAMIENTO ALCALINO ; Use and upgrading of agricultural and food by-products. Biotechnology ; WASTES ; YIELDS</subject><ispartof>Journal of agricultural and food chemistry, 1998-08, Vol.46 (8), p.3359-3364</ispartof><rights>Copyright © 1998 American Chemical Society</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a381t-ef5cd652e3d35b5cf388fdbf5fcf8d76d977463e9c40fcefcce7f763dbabf0ea3</citedby><cites>FETCH-LOGICAL-a381t-ef5cd652e3d35b5cf388fdbf5fcf8d76d977463e9c40fcefcce7f763dbabf0ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf971098c$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf971098c$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2362717$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Umikalsom, Md S</creatorcontrib><creatorcontrib>Ariff, Arbakariya B</creatorcontrib><creatorcontrib>Karim, Mohamed I. A</creatorcontrib><title>Saccharification of Pretreated Oil Palm Empty Fruit Bunch Fiber Using Cellulase of Chaetomium globosum</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>The effectiveness of different chemical and physical pretreatments to alter cellulose structure and to reduce hemicellullose and lignin contents in oil palm empty fruit bunch (OPEFB) fibers for subsequent use as substrate for enzymatic saccharification was studied. The saccharification was carried out using concentrated cellulase preparation from culture broth of Chaetomium globosum strain 414 containing 10 U/mL FPase, 285 U/mL CMCase, and 60 U/mL β-glucosidase. The use of 0.5% NaOH to treat OPEFB by soaking at 30 °C for 4 h gave the highest rate and degree of hydrolysis followed by 0.5% HNO3, HCl, EDA, and EDTA. Autoclaving the chemically treated OPEFB fiber at 121 °C for 5 min improved the hydrolysis by 2-fold. The improvement in hydrolysis was related to a decrease in the hemicellulose and lignin contents and an increase in the cellulose content. The qualitative hydrolysis yield for autoclaved OPEFB fiber treated with 2% NaOH was 85.9%. During saccharification of OPEFB using cellulase of C. globosum, the amount of glucose produced was higher while the amount of cellobiose produced was lower than those obtained with commercial cellulase of Trichoderma viride. Keywords: Cellulase; endoglucanase; cellobiohydrolase; β-glucosidase; Chaetomium globosum; cellulosic material; oil palm empty fruit bunch</description><subject>ALKALI TREATMENT</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>CELLULASE</subject><subject>CELLULOSE</subject><subject>CELULASA</subject><subject>CELULOSA</subject><subject>CHAETOMIUM GLOBOSUM</subject><subject>DECHET</subject><subject>DESECHOS</subject><subject>DIETARY FIBRES</subject><subject>ENZYMATIC SACCHARIFICATION</subject><subject>ESTERILIZACION TERMICA</subject><subject>FIBRA DE LA DIETA</subject><subject>FIBRE ALIMENTAIRE</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HEAT STERILIZING</subject><subject>HEMICELLULOSE</subject><subject>HEMICELULOSA</subject><subject>HIDROLISIS</subject><subject>HYDROLYSE</subject><subject>HYDROLYSIS</subject><subject>LIGNINAS</subject><subject>LIGNINE</subject><subject>LIGNINS</subject><subject>LIGNOCELLULOSE</subject><subject>LIGNOCELULOSA</subject><subject>Methods. Procedures. Technologies</subject><subject>OIL PALMS</subject><subject>PALMAS OLEAGINOSAS</subject><subject>PALMIER OLEIFERE</subject><subject>PRETREATMENT</subject><subject>PRODUCTIVIDAD</subject><subject>PRODUCTIVITE</subject><subject>PRODUCTIVITY</subject><subject>RENDEMENT</subject><subject>RENDIMIENTO</subject><subject>STERILISATION THERMIQUE</subject><subject>TRAITEMENT AUX ALCALIS</subject><subject>TRATAMIENTO ALCALINO</subject><subject>Use and upgrading of agricultural and food by-products. Biotechnology</subject><subject>WASTES</subject><subject>YIELDS</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNptkEtLAzEUhYMoWB8Lf4CQhS5cjCYTM5lZamlVsFioRXAT7mRy29R5lGQG7L93ZKQrV3fxfedwOYRccHbLWczvNpgpzrLUHJARlzGLJOfpIRmxHkapTPgxOQlhwxhLpWIjggswZg3eoTPQuqamDdK5t6230NqCvrmSzqGs6KTatjs69Z1r6WNXmzWdutx6ugyuXtGxLcuuhGB_4-M12LapXFfRVdnkTeiqM3KEUAZ7_ndPyXI6eR8_R69vTy_jh9cIRMrbyKI0RSJjKwohc2lQpCkWOUo0mBYqKTKl7hNhM3PP0Fg0xipUiShyyJFZEKfkZug1vgnBW9Rb7yrwO82Z_h1I7wfq3avB3UIwUKKH2riwD8QiiRVXvRYNmgut_d5j8F86UUJJ_T5f6OePT8Xl7EPPev9y8BEaDSvfVy4XPMsyxrhgcc-vBw4m6E3T-bof5J_3fgCPSYvH</recordid><startdate>19980817</startdate><enddate>19980817</enddate><creator>Umikalsom, Md S</creator><creator>Ariff, Arbakariya B</creator><creator>Karim, Mohamed I. A</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19980817</creationdate><title>Saccharification of Pretreated Oil Palm Empty Fruit Bunch Fiber Using Cellulase of Chaetomium globosum</title><author>Umikalsom, Md S ; Ariff, Arbakariya B ; Karim, Mohamed I. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a381t-ef5cd652e3d35b5cf388fdbf5fcf8d76d977463e9c40fcefcce7f763dbabf0ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>ALKALI TREATMENT</topic><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>CELLULASE</topic><topic>CELLULOSE</topic><topic>CELULASA</topic><topic>CELULOSA</topic><topic>CHAETOMIUM GLOBOSUM</topic><topic>DECHET</topic><topic>DESECHOS</topic><topic>DIETARY FIBRES</topic><topic>ENZYMATIC SACCHARIFICATION</topic><topic>ESTERILIZACION TERMICA</topic><topic>FIBRA DE LA DIETA</topic><topic>FIBRE ALIMENTAIRE</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HEAT STERILIZING</topic><topic>HEMICELLULOSE</topic><topic>HEMICELULOSA</topic><topic>HIDROLISIS</topic><topic>HYDROLYSE</topic><topic>HYDROLYSIS</topic><topic>LIGNINAS</topic><topic>LIGNINE</topic><topic>LIGNINS</topic><topic>LIGNOCELLULOSE</topic><topic>LIGNOCELULOSA</topic><topic>Methods. Procedures. Technologies</topic><topic>OIL PALMS</topic><topic>PALMAS OLEAGINOSAS</topic><topic>PALMIER OLEIFERE</topic><topic>PRETREATMENT</topic><topic>PRODUCTIVIDAD</topic><topic>PRODUCTIVITE</topic><topic>PRODUCTIVITY</topic><topic>RENDEMENT</topic><topic>RENDIMIENTO</topic><topic>STERILISATION THERMIQUE</topic><topic>TRAITEMENT AUX ALCALIS</topic><topic>TRATAMIENTO ALCALINO</topic><topic>Use and upgrading of agricultural and food by-products. Biotechnology</topic><topic>WASTES</topic><topic>YIELDS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Umikalsom, Md S</creatorcontrib><creatorcontrib>Ariff, Arbakariya B</creatorcontrib><creatorcontrib>Karim, Mohamed I. A</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Umikalsom, Md S</au><au>Ariff, Arbakariya B</au><au>Karim, Mohamed I. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Saccharification of Pretreated Oil Palm Empty Fruit Bunch Fiber Using Cellulase of Chaetomium globosum</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>1998-08-17</date><risdate>1998</risdate><volume>46</volume><issue>8</issue><spage>3359</spage><epage>3364</epage><pages>3359-3364</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>The effectiveness of different chemical and physical pretreatments to alter cellulose structure and to reduce hemicellullose and lignin contents in oil palm empty fruit bunch (OPEFB) fibers for subsequent use as substrate for enzymatic saccharification was studied. The saccharification was carried out using concentrated cellulase preparation from culture broth of Chaetomium globosum strain 414 containing 10 U/mL FPase, 285 U/mL CMCase, and 60 U/mL β-glucosidase. The use of 0.5% NaOH to treat OPEFB by soaking at 30 °C for 4 h gave the highest rate and degree of hydrolysis followed by 0.5% HNO3, HCl, EDA, and EDTA. Autoclaving the chemically treated OPEFB fiber at 121 °C for 5 min improved the hydrolysis by 2-fold. The improvement in hydrolysis was related to a decrease in the hemicellulose and lignin contents and an increase in the cellulose content. The qualitative hydrolysis yield for autoclaved OPEFB fiber treated with 2% NaOH was 85.9%. During saccharification of OPEFB using cellulase of C. globosum, the amount of glucose produced was higher while the amount of cellobiose produced was lower than those obtained with commercial cellulase of Trichoderma viride. Keywords: Cellulase; endoglucanase; cellobiohydrolase; β-glucosidase; Chaetomium globosum; cellulosic material; oil palm empty fruit bunch</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/jf971098c</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 1998-08, Vol.46 (8), p.3359-3364 |
| issn | 0021-8561 1520-5118 |
| language | eng |
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| source | ACS Publications |
| subjects | ALKALI TREATMENT Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology CELLULASE CELLULOSE CELULASA CELULOSA CHAETOMIUM GLOBOSUM DECHET DESECHOS DIETARY FIBRES ENZYMATIC SACCHARIFICATION ESTERILIZACION TERMICA FIBRA DE LA DIETA FIBRE ALIMENTAIRE Food industries Fundamental and applied biological sciences. Psychology HEAT STERILIZING HEMICELLULOSE HEMICELULOSA HIDROLISIS HYDROLYSE HYDROLYSIS LIGNINAS LIGNINE LIGNINS LIGNOCELLULOSE LIGNOCELULOSA Methods. Procedures. Technologies OIL PALMS PALMAS OLEAGINOSAS PALMIER OLEIFERE PRETREATMENT PRODUCTIVIDAD PRODUCTIVITE PRODUCTIVITY RENDEMENT RENDIMIENTO STERILISATION THERMIQUE TRAITEMENT AUX ALCALIS TRATAMIENTO ALCALINO Use and upgrading of agricultural and food by-products. Biotechnology WASTES YIELDS |
| title | Saccharification of Pretreated Oil Palm Empty Fruit Bunch Fiber Using Cellulase of Chaetomium globosum |
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