Enzymatic Release of Antioxidants for Human Low-Density Lipoprotein from Grape Pomace
Enzyme-assisted release of phenolic antioxidants from grape pomace from wine production was examined. The enzymes used were Grindamyl pectinase from Aspergillus niger and Celluclast from Trichoderma reesei. Total phenols released ranged from 820 to 6055 mg/L gallic acid equivalents (GAE) and varied...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 1998-07, Vol.46 (7), p.2439-2446 |
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| creator | Meyer, Anne S Jepsen, Signe M Sørensen, Nina S |
| description | Enzyme-assisted release of phenolic antioxidants from grape pomace from wine production was examined. The enzymes used were Grindamyl pectinase from Aspergillus niger and Celluclast from Trichoderma reesei. Total phenols released ranged from 820 to 6055 mg/L gallic acid equivalents (GAE) and varied in response to enzyme type, time of enzyme treatment, particle size of the pomace, and type of extraction solvent employed. The yield of total phenols was correlated to the degree of plant cell wall breakdown of grape pomace (r > 0.6, P < 0.01). Grindamyl pectinase catalyzed degradation of grape pomace polysaccharides (P < 0.001), whereas Celluclast did not. Reduction of the particle size of grape pomace to 125−250 μm increased the enzymatic polysaccharide hydrolysis and the recovery of phenols. The grape pomace extracts significantly retarded human low-density lipoprotein oxidation in vitro. When evaluated at 3.0 μM GAE, phenolic extracts of Grindamyl pectinase treated pomace of small particle size (125−250 μm) appeared to release more active antioxidant phenols than the other types of enzyme treatments tested (P < 0.05). Keywords: Pectinase; cellulase; phenols; plant cell wall degradation; LDL oxidation |
| doi_str_mv | 10.1021/jf971012f |
| format | Article |
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The enzymes used were Grindamyl pectinase from Aspergillus niger and Celluclast from Trichoderma reesei. Total phenols released ranged from 820 to 6055 mg/L gallic acid equivalents (GAE) and varied in response to enzyme type, time of enzyme treatment, particle size of the pomace, and type of extraction solvent employed. The yield of total phenols was correlated to the degree of plant cell wall breakdown of grape pomace (r > 0.6, P < 0.01). Grindamyl pectinase catalyzed degradation of grape pomace polysaccharides (P < 0.001), whereas Celluclast did not. Reduction of the particle size of grape pomace to 125−250 μm increased the enzymatic polysaccharide hydrolysis and the recovery of phenols. The grape pomace extracts significantly retarded human low-density lipoprotein oxidation in vitro. When evaluated at 3.0 μM GAE, phenolic extracts of Grindamyl pectinase treated pomace of small particle size (125−250 μm) appeared to release more active antioxidant phenols than the other types of enzyme treatments tested (P < 0.05). Keywords: Pectinase; cellulase; phenols; plant cell wall degradation; LDL oxidation</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf971012f</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>ANTIOXIDANTES ; ANTIOXIDANTS ; ANTIOXYDANT ; ASPERGILLUS NIGER ; Biological and medical sciences ; BYPRODUCTS ; CELL WALLS ; CELLULASE ; CELULASA ; COMPOSE PHENOLIQUE ; COMPUESTOS FENOLICOS ; CORRELATION ; DOSAGE EFFECTS ; EFECTOS DE DOSIFICACION ; EFFET DOSE ; ENZYME TREATMENT ; EXPERIMENTACION IN VITRO ; EXPERIMENTATION IN VITRO ; General pharmacology ; GENERO HUMANO ; GENRE HUMAIN ; GRAPEVINES ; GROSSEUR DES PARTICULES ; HIDROLISIS ; HYDROLYSE ; HYDROLYSIS ; IN VITRO EXPERIMENTATION ; LIPOPROTEINAS ; LIPOPROTEINE ; LIPOPROTEINS ; MANKIND ; Medical sciences ; OXIDACION ; OXIDATION ; OXYDATION ; PARED CELULAR ; PAROI CELLULAIRE ; PARTICLE SIZE ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; PHENOLIC COMPOUNDS ; PLANT CELL WALL DEGRADATION ; POLIGALACTURONASA ; POLISACARIDOS ; POLYGALACTURONASE ; POLYHOLOSIDE ; POLYSACCHARIDES ; RENDEMENT ; RENDIMIENTO ; SOUS PRODUIT ; SUBPRODUCTOS ; TAMANO DE LA PARTICULA ; TRICHODERMA LONGIBRACHIATUM ; VID ; VIGNE ; YIELDS</subject><ispartof>Journal of agricultural and food chemistry, 1998-07, Vol.46 (7), p.2439-2446</ispartof><rights>Copyright © 1998 American Chemical Society</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a412t-dd2f84211bf50e8153d3991e8d544d9cb576110ca1b291713c1a92610d6a4b473</citedby><cites>FETCH-LOGICAL-a412t-dd2f84211bf50e8153d3991e8d544d9cb576110ca1b291713c1a92610d6a4b473</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf971012f$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf971012f$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27074,27922,27923,56736,56786</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2333307$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Meyer, Anne S</creatorcontrib><creatorcontrib>Jepsen, Signe M</creatorcontrib><creatorcontrib>Sørensen, Nina S</creatorcontrib><title>Enzymatic Release of Antioxidants for Human Low-Density Lipoprotein from Grape Pomace</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Enzyme-assisted release of phenolic antioxidants from grape pomace from wine production was examined. The enzymes used were Grindamyl pectinase from Aspergillus niger and Celluclast from Trichoderma reesei. Total phenols released ranged from 820 to 6055 mg/L gallic acid equivalents (GAE) and varied in response to enzyme type, time of enzyme treatment, particle size of the pomace, and type of extraction solvent employed. The yield of total phenols was correlated to the degree of plant cell wall breakdown of grape pomace (r > 0.6, P < 0.01). Grindamyl pectinase catalyzed degradation of grape pomace polysaccharides (P < 0.001), whereas Celluclast did not. Reduction of the particle size of grape pomace to 125−250 μm increased the enzymatic polysaccharide hydrolysis and the recovery of phenols. The grape pomace extracts significantly retarded human low-density lipoprotein oxidation in vitro. When evaluated at 3.0 μM GAE, phenolic extracts of Grindamyl pectinase treated pomace of small particle size (125−250 μm) appeared to release more active antioxidant phenols than the other types of enzyme treatments tested (P < 0.05). Keywords: Pectinase; cellulase; phenols; plant cell wall degradation; LDL oxidation</description><subject>ANTIOXIDANTES</subject><subject>ANTIOXIDANTS</subject><subject>ANTIOXYDANT</subject><subject>ASPERGILLUS NIGER</subject><subject>Biological and medical sciences</subject><subject>BYPRODUCTS</subject><subject>CELL WALLS</subject><subject>CELLULASE</subject><subject>CELULASA</subject><subject>COMPOSE PHENOLIQUE</subject><subject>COMPUESTOS FENOLICOS</subject><subject>CORRELATION</subject><subject>DOSAGE EFFECTS</subject><subject>EFECTOS DE DOSIFICACION</subject><subject>EFFET DOSE</subject><subject>ENZYME TREATMENT</subject><subject>EXPERIMENTACION IN VITRO</subject><subject>EXPERIMENTATION IN VITRO</subject><subject>General pharmacology</subject><subject>GENERO HUMANO</subject><subject>GENRE HUMAIN</subject><subject>GRAPEVINES</subject><subject>GROSSEUR DES PARTICULES</subject><subject>HIDROLISIS</subject><subject>HYDROLYSE</subject><subject>HYDROLYSIS</subject><subject>IN VITRO EXPERIMENTATION</subject><subject>LIPOPROTEINAS</subject><subject>LIPOPROTEINE</subject><subject>LIPOPROTEINS</subject><subject>MANKIND</subject><subject>Medical sciences</subject><subject>OXIDACION</subject><subject>OXIDATION</subject><subject>OXYDATION</subject><subject>PARED CELULAR</subject><subject>PAROI CELLULAIRE</subject><subject>PARTICLE SIZE</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>PHENOLIC COMPOUNDS</subject><subject>PLANT CELL WALL DEGRADATION</subject><subject>POLIGALACTURONASA</subject><subject>POLISACARIDOS</subject><subject>POLYGALACTURONASE</subject><subject>POLYHOLOSIDE</subject><subject>POLYSACCHARIDES</subject><subject>RENDEMENT</subject><subject>RENDIMIENTO</subject><subject>SOUS PRODUIT</subject><subject>SUBPRODUCTOS</subject><subject>TAMANO DE LA PARTICULA</subject><subject>TRICHODERMA LONGIBRACHIATUM</subject><subject>VID</subject><subject>VIGNE</subject><subject>YIELDS</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNptkMFO4zAQhi20SHQLBx4AyQc4cAjrceI4PlbdbkGqAFF6tqaOjdxt4sgOWsrTb1BWPe1c5vB_82v0EXIJ7A4Yhx87pyQw4O6ETEBwlgmA6huZsCHMKlHCGfme0o4xVgnJJmSzaD8PDfbe0Be7t5gsDY7O2t6HD19j2yfqQqT37w22dBX-ZD9tm3x_oCvfhS6G3vqWuhgauozYWfocGjT2nJw63Cd78W9PyebX4nV-n62elg_z2SrDAnif1TV3VcEBtk4wW4HI61wpsFUtiqJWZitkCcAMwpYrkJAbQMVLYHWJxbaQ-ZTcjr0mhpSidbqLvsF40MD0lw999DGw1yPbYTK4dxFb49PxgOfDsK_KbMR86u3HMcb4W5cyl0K_Pq-1LEUh1o9SPw781cg7DBrf4lC5WYNSalAshpen5GbM0SS9C--xHYT8572_ftCCSA</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>Meyer, Anne S</creator><creator>Jepsen, Signe M</creator><creator>Sørensen, Nina S</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19980701</creationdate><title>Enzymatic Release of Antioxidants for Human Low-Density Lipoprotein from Grape Pomace</title><author>Meyer, Anne S ; Jepsen, Signe M ; Sørensen, Nina S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a412t-dd2f84211bf50e8153d3991e8d544d9cb576110ca1b291713c1a92610d6a4b473</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>ANTIOXIDANTES</topic><topic>ANTIOXIDANTS</topic><topic>ANTIOXYDANT</topic><topic>ASPERGILLUS NIGER</topic><topic>Biological and medical sciences</topic><topic>BYPRODUCTS</topic><topic>CELL WALLS</topic><topic>CELLULASE</topic><topic>CELULASA</topic><topic>COMPOSE PHENOLIQUE</topic><topic>COMPUESTOS FENOLICOS</topic><topic>CORRELATION</topic><topic>DOSAGE EFFECTS</topic><topic>EFECTOS DE DOSIFICACION</topic><topic>EFFET DOSE</topic><topic>ENZYME TREATMENT</topic><topic>EXPERIMENTACION IN VITRO</topic><topic>EXPERIMENTATION IN VITRO</topic><topic>General pharmacology</topic><topic>GENERO HUMANO</topic><topic>GENRE HUMAIN</topic><topic>GRAPEVINES</topic><topic>GROSSEUR DES PARTICULES</topic><topic>HIDROLISIS</topic><topic>HYDROLYSE</topic><topic>HYDROLYSIS</topic><topic>IN VITRO EXPERIMENTATION</topic><topic>LIPOPROTEINAS</topic><topic>LIPOPROTEINE</topic><topic>LIPOPROTEINS</topic><topic>MANKIND</topic><topic>Medical sciences</topic><topic>OXIDACION</topic><topic>OXIDATION</topic><topic>OXYDATION</topic><topic>PARED CELULAR</topic><topic>PAROI CELLULAIRE</topic><topic>PARTICLE SIZE</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>PHENOLIC COMPOUNDS</topic><topic>PLANT CELL WALL DEGRADATION</topic><topic>POLIGALACTURONASA</topic><topic>POLISACARIDOS</topic><topic>POLYGALACTURONASE</topic><topic>POLYHOLOSIDE</topic><topic>POLYSACCHARIDES</topic><topic>RENDEMENT</topic><topic>RENDIMIENTO</topic><topic>SOUS PRODUIT</topic><topic>SUBPRODUCTOS</topic><topic>TAMANO DE LA PARTICULA</topic><topic>TRICHODERMA LONGIBRACHIATUM</topic><topic>VID</topic><topic>VIGNE</topic><topic>YIELDS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Meyer, Anne S</creatorcontrib><creatorcontrib>Jepsen, Signe M</creatorcontrib><creatorcontrib>Sørensen, Nina S</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Meyer, Anne S</au><au>Jepsen, Signe M</au><au>Sørensen, Nina S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzymatic Release of Antioxidants for Human Low-Density Lipoprotein from Grape Pomace</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>1998-07-01</date><risdate>1998</risdate><volume>46</volume><issue>7</issue><spage>2439</spage><epage>2446</epage><pages>2439-2446</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Enzyme-assisted release of phenolic antioxidants from grape pomace from wine production was examined. The enzymes used were Grindamyl pectinase from Aspergillus niger and Celluclast from Trichoderma reesei. Total phenols released ranged from 820 to 6055 mg/L gallic acid equivalents (GAE) and varied in response to enzyme type, time of enzyme treatment, particle size of the pomace, and type of extraction solvent employed. The yield of total phenols was correlated to the degree of plant cell wall breakdown of grape pomace (r > 0.6, P < 0.01). Grindamyl pectinase catalyzed degradation of grape pomace polysaccharides (P < 0.001), whereas Celluclast did not. Reduction of the particle size of grape pomace to 125−250 μm increased the enzymatic polysaccharide hydrolysis and the recovery of phenols. The grape pomace extracts significantly retarded human low-density lipoprotein oxidation in vitro. When evaluated at 3.0 μM GAE, phenolic extracts of Grindamyl pectinase treated pomace of small particle size (125−250 μm) appeared to release more active antioxidant phenols than the other types of enzyme treatments tested (P < 0.05). Keywords: Pectinase; cellulase; phenols; plant cell wall degradation; LDL oxidation</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/jf971012f</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 1998-07, Vol.46 (7), p.2439-2446 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_crossref_primary_10_1021_jf971012f |
| source | American Chemical Society Journals |
| subjects | ANTIOXIDANTES ANTIOXIDANTS ANTIOXYDANT ASPERGILLUS NIGER Biological and medical sciences BYPRODUCTS CELL WALLS CELLULASE CELULASA COMPOSE PHENOLIQUE COMPUESTOS FENOLICOS CORRELATION DOSAGE EFFECTS EFECTOS DE DOSIFICACION EFFET DOSE ENZYME TREATMENT EXPERIMENTACION IN VITRO EXPERIMENTATION IN VITRO General pharmacology GENERO HUMANO GENRE HUMAIN GRAPEVINES GROSSEUR DES PARTICULES HIDROLISIS HYDROLYSE HYDROLYSIS IN VITRO EXPERIMENTATION LIPOPROTEINAS LIPOPROTEINE LIPOPROTEINS MANKIND Medical sciences OXIDACION OXIDATION OXYDATION PARED CELULAR PAROI CELLULAIRE PARTICLE SIZE Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments PHENOLIC COMPOUNDS PLANT CELL WALL DEGRADATION POLIGALACTURONASA POLISACARIDOS POLYGALACTURONASE POLYHOLOSIDE POLYSACCHARIDES RENDEMENT RENDIMIENTO SOUS PRODUIT SUBPRODUCTOS TAMANO DE LA PARTICULA TRICHODERMA LONGIBRACHIATUM VID VIGNE YIELDS |
| title | Enzymatic Release of Antioxidants for Human Low-Density Lipoprotein from Grape Pomace |
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