Extraction and Separation of the Bright-Greenish-Yellow Fluorescent Material from Aflatoxigenic Aspergillus spp. Infected Cotton Lint by HPLC−UV/FL
To isolate the bright-greenish-yellow-fluorescence (BGY-F) material associated with aflatoxin contamination on cotton lint, various in vitro chemical and in vivo natural BGY-F reaction products were prepared. BGY-F material was obtained from reactions involving (1) kojic acid (KA) and H2O2, (b) KA,...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 1998-03, Vol.46 (3), p.1071-1075 |
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| creator | Zeringue, H. J Shih, B. Y |
| description | To isolate the bright-greenish-yellow-fluorescence (BGY-F) material associated with aflatoxin contamination on cotton lint, various in vitro chemical and in vivo natural BGY-F reaction products were prepared. BGY-F material was obtained from reactions involving (1) kojic acid (KA) and H2O2, (b) KA, peroxidase, and H2O2, (c) fresh cotton locules treated with KA and H2O2, (d) detached cotton locules inoculated with an aflatoxigenic Aspergillus flavus spore suspension, and (e) live developing cotton bolls inoculated with aflatoxigenic A. flavus. BGY-F preparations separated on TLC resulted in the same R f value (0.52), indicating the possibility of the same compound. Under HPLC conditions, at a 380 nm wavelength setting, BGY-F retention times ranged 5.74−6.09 min; under fluorescent detection at 435 nm (excitation) and 494 nm (emission), retention times ranged from 5.75 to 6.09 min. Apparently, in all methods used to form the BGY-F compound, both in vivo and in vitro, only one compound with specific chromatographic characteristics was produced, and the product is likely an oxidized form of KA. Keywords: Cotton; Gossypium hirsutum L.; bright-greenish-yellow fluorescence (BGY-F); Aspergillus flavus; aflatoxin; HPLC − UV/F |
| doi_str_mv | 10.1021/jf9707391 |
| format | Article |
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J ; Shih, B. Y</creator><creatorcontrib>Zeringue, H. J ; Shih, B. Y</creatorcontrib><description>To isolate the bright-greenish-yellow-fluorescence (BGY-F) material associated with aflatoxin contamination on cotton lint, various in vitro chemical and in vivo natural BGY-F reaction products were prepared. BGY-F material was obtained from reactions involving (1) kojic acid (KA) and H2O2, (b) KA, peroxidase, and H2O2, (c) fresh cotton locules treated with KA and H2O2, (d) detached cotton locules inoculated with an aflatoxigenic Aspergillus flavus spore suspension, and (e) live developing cotton bolls inoculated with aflatoxigenic A. flavus. BGY-F preparations separated on TLC resulted in the same R f value (0.52), indicating the possibility of the same compound. Under HPLC conditions, at a 380 nm wavelength setting, BGY-F retention times ranged 5.74−6.09 min; under fluorescent detection at 435 nm (excitation) and 494 nm (emission), retention times ranged from 5.75 to 6.09 min. Apparently, in all methods used to form the BGY-F compound, both in vivo and in vitro, only one compound with specific chromatographic characteristics was produced, and the product is likely an oxidized form of KA. Keywords: Cotton; Gossypium hirsutum L.; bright-greenish-yellow fluorescence (BGY-F); Aspergillus flavus; aflatoxin; HPLC − UV/F</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf9707391</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>AFLATOXINS ; ASPERGILLUS FLAVUS ; Biological and medical sciences ; BIOLOGICAL CONTAMINATION ; BOLLS ; CHEMICAL REACTIONS ; EXTRACTION ; FLUORESCENCE ; FRUIT ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Generalities. Techniques ; GOSSYPIUM ; GOSSYPIUM HIRSUTUM ; HPLC ; HYDROGEN PEROXIDE ; ISOLATION ; KOJIC ACID ; Medical sciences ; MICROBIAL CONTAMINATION ; Microbiology ; Mycology ; Pathogenicity, host-agent relations, miscellaneous strains, epidemiology ; Phytopathology. Animal pests. Plant and forest protection ; Plant poisons toxicology ; SEPARATING ; Toxicology ; ULTRAVIOLET RADIATION</subject><ispartof>Journal of agricultural and food chemistry, 1998-03, Vol.46 (3), p.1071-1075</ispartof><rights>Copyright © 1998 American Chemical Society</rights><rights>1998 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a412t-fd23540d7991d4d8ecca8b4dc547c883cb595250f7cabea257ead36eb6c1d2e13</citedby><cites>FETCH-LOGICAL-a412t-fd23540d7991d4d8ecca8b4dc547c883cb595250f7cabea257ead36eb6c1d2e13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf9707391$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf9707391$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2202578$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Zeringue, H. J</creatorcontrib><creatorcontrib>Shih, B. Y</creatorcontrib><title>Extraction and Separation of the Bright-Greenish-Yellow Fluorescent Material from Aflatoxigenic Aspergillus spp. Infected Cotton Lint by HPLC−UV/FL</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>To isolate the bright-greenish-yellow-fluorescence (BGY-F) material associated with aflatoxin contamination on cotton lint, various in vitro chemical and in vivo natural BGY-F reaction products were prepared. BGY-F material was obtained from reactions involving (1) kojic acid (KA) and H2O2, (b) KA, peroxidase, and H2O2, (c) fresh cotton locules treated with KA and H2O2, (d) detached cotton locules inoculated with an aflatoxigenic Aspergillus flavus spore suspension, and (e) live developing cotton bolls inoculated with aflatoxigenic A. flavus. BGY-F preparations separated on TLC resulted in the same R f value (0.52), indicating the possibility of the same compound. Under HPLC conditions, at a 380 nm wavelength setting, BGY-F retention times ranged 5.74−6.09 min; under fluorescent detection at 435 nm (excitation) and 494 nm (emission), retention times ranged from 5.75 to 6.09 min. Apparently, in all methods used to form the BGY-F compound, both in vivo and in vitro, only one compound with specific chromatographic characteristics was produced, and the product is likely an oxidized form of KA. Keywords: Cotton; Gossypium hirsutum L.; bright-greenish-yellow fluorescence (BGY-F); Aspergillus flavus; aflatoxin; HPLC − UV/F</description><subject>AFLATOXINS</subject><subject>ASPERGILLUS FLAVUS</subject><subject>Biological and medical sciences</subject><subject>BIOLOGICAL CONTAMINATION</subject><subject>BOLLS</subject><subject>CHEMICAL REACTIONS</subject><subject>EXTRACTION</subject><subject>FLUORESCENCE</subject><subject>FRUIT</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Generalities. Techniques</subject><subject>GOSSYPIUM</subject><subject>GOSSYPIUM HIRSUTUM</subject><subject>HPLC</subject><subject>HYDROGEN PEROXIDE</subject><subject>ISOLATION</subject><subject>KOJIC ACID</subject><subject>Medical sciences</subject><subject>MICROBIAL CONTAMINATION</subject><subject>Microbiology</subject><subject>Mycology</subject><subject>Pathogenicity, host-agent relations, miscellaneous strains, epidemiology</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant poisons toxicology</subject><subject>SEPARATING</subject><subject>Toxicology</subject><subject>ULTRAVIOLET RADIATION</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNptkEFv0zAYhiMEEmVw2A9A8gEOHLLZSVwnx65a10kBJrpO4mR9cT63Llkc2a7o_sHOk_iD_BI8gnriYFnW-_j5Pr1JcsroGaMZO9_pSlCRV-xFMmE8oylnrHyZTGgM05JP2evkjfc7SmnJBZ0kvy4PwYEKxvYE-pascAAHf59Wk7BFcuHMZhvSK4fYG79Nv2PX2Z9k0e2tQ6-wD-QzBHQGOqKdvScz3UGwB7OJvCIzP6DbmK7be-KH4Yxc9xpVwJbMbQhxTG2ioXkgy5t6_vvxaX13vqjfJq80dB7f_btPkvXi8na-TOuvV9fzWZ1CwbKQ6jbLeUFbUVWsLdoSlYKyKVrFC6HKMlcNr3jGqRYKGoSMC4Q2n2IzVazNkOUnyafRq5z13qGWgzP34B4ko_K5T3nsM7IfRnYAr6DTDnpl_PFDltHoLyOWjpjxAQ_HGNwPORW54PL2ZhVP9e3Lsr6Qz9r3I6_BSti4qFyvWBWnlnkuRMw_jjkoL3d27_pYyH_W-wNQdZvw</recordid><startdate>19980301</startdate><enddate>19980301</enddate><creator>Zeringue, H. J</creator><creator>Shih, B. Y</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>19980301</creationdate><title>Extraction and Separation of the Bright-Greenish-Yellow Fluorescent Material from Aflatoxigenic Aspergillus spp. Infected Cotton Lint by HPLC−UV/FL</title><author>Zeringue, H. J ; Shih, B. Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a412t-fd23540d7991d4d8ecca8b4dc547c883cb595250f7cabea257ead36eb6c1d2e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>AFLATOXINS</topic><topic>ASPERGILLUS FLAVUS</topic><topic>Biological and medical sciences</topic><topic>BIOLOGICAL CONTAMINATION</topic><topic>BOLLS</topic><topic>CHEMICAL REACTIONS</topic><topic>EXTRACTION</topic><topic>FLUORESCENCE</topic><topic>FRUIT</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Generalities. Techniques</topic><topic>GOSSYPIUM</topic><topic>GOSSYPIUM HIRSUTUM</topic><topic>HPLC</topic><topic>HYDROGEN PEROXIDE</topic><topic>ISOLATION</topic><topic>KOJIC ACID</topic><topic>Medical sciences</topic><topic>MICROBIAL CONTAMINATION</topic><topic>Microbiology</topic><topic>Mycology</topic><topic>Pathogenicity, host-agent relations, miscellaneous strains, epidemiology</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>Plant poisons toxicology</topic><topic>SEPARATING</topic><topic>Toxicology</topic><topic>ULTRAVIOLET RADIATION</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zeringue, H. J</creatorcontrib><creatorcontrib>Shih, B. Y</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zeringue, H. J</au><au>Shih, B. Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extraction and Separation of the Bright-Greenish-Yellow Fluorescent Material from Aflatoxigenic Aspergillus spp. Infected Cotton Lint by HPLC−UV/FL</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>1998-03-01</date><risdate>1998</risdate><volume>46</volume><issue>3</issue><spage>1071</spage><epage>1075</epage><pages>1071-1075</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>To isolate the bright-greenish-yellow-fluorescence (BGY-F) material associated with aflatoxin contamination on cotton lint, various in vitro chemical and in vivo natural BGY-F reaction products were prepared. BGY-F material was obtained from reactions involving (1) kojic acid (KA) and H2O2, (b) KA, peroxidase, and H2O2, (c) fresh cotton locules treated with KA and H2O2, (d) detached cotton locules inoculated with an aflatoxigenic Aspergillus flavus spore suspension, and (e) live developing cotton bolls inoculated with aflatoxigenic A. flavus. BGY-F preparations separated on TLC resulted in the same R f value (0.52), indicating the possibility of the same compound. Under HPLC conditions, at a 380 nm wavelength setting, BGY-F retention times ranged 5.74−6.09 min; under fluorescent detection at 435 nm (excitation) and 494 nm (emission), retention times ranged from 5.75 to 6.09 min. Apparently, in all methods used to form the BGY-F compound, both in vivo and in vitro, only one compound with specific chromatographic characteristics was produced, and the product is likely an oxidized form of KA. Keywords: Cotton; Gossypium hirsutum L.; bright-greenish-yellow fluorescence (BGY-F); Aspergillus flavus; aflatoxin; HPLC − UV/F</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><doi>10.1021/jf9707391</doi><tpages>5</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 1998-03, Vol.46 (3), p.1071-1075 |
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| language | eng |
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| source | American Chemical Society Journals |
| subjects | AFLATOXINS ASPERGILLUS FLAVUS Biological and medical sciences BIOLOGICAL CONTAMINATION BOLLS CHEMICAL REACTIONS EXTRACTION FLUORESCENCE FRUIT Fundamental and applied biological sciences. Psychology Fungal plant pathogens Generalities. Techniques GOSSYPIUM GOSSYPIUM HIRSUTUM HPLC HYDROGEN PEROXIDE ISOLATION KOJIC ACID Medical sciences MICROBIAL CONTAMINATION Microbiology Mycology Pathogenicity, host-agent relations, miscellaneous strains, epidemiology Phytopathology. Animal pests. Plant and forest protection Plant poisons toxicology SEPARATING Toxicology ULTRAVIOLET RADIATION |
| title | Extraction and Separation of the Bright-Greenish-Yellow Fluorescent Material from Aflatoxigenic Aspergillus spp. Infected Cotton Lint by HPLC−UV/FL |
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