Development of a Mab-Based Heterologous Immunoassay for the Broad-Selective Determination of Organophosphorus Pesticides
A broad-selective monoclonal antibody (Mab) for organophosphorus (OP) pesticides was raised using heterologous indirect enzyme-linked immunosorbent assay (ELISA) to screen hybridomas. On the basis of this Mab, five coating antigens were used to develop homologous and heterologous indirect competitiv...
Gespeichert in:
| Veröffentlicht in: | Journal of agricultural and food chemistry 2010-05, Vol.58 (9), p.5658-5663 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5663 |
|---|---|
| container_issue | 9 |
| container_start_page | 5658 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 58 |
| creator | Wang, Chunmei Li, Xianbin Liu, Yihua Guo, Yirong Xie, Rong Gui, Wenjun Zhu, Guonian |
| description | A broad-selective monoclonal antibody (Mab) for organophosphorus (OP) pesticides was raised using heterologous indirect enzyme-linked immunosorbent assay (ELISA) to screen hybridomas. On the basis of this Mab, five coating antigens were used to develop homologous and heterologous indirect competitive ELISAs. With the most suitable competitor, a sensitive and broad-selective ELISA was developed. The IC50 values were estimated to be 20.32 ng/mL for parathion, 21.44 ng/mL for methyl-parathion, 42.15 ng/mL for fenitrothion, and 58.85 ng/mL for isocarbophos. Spike recoveries were between 70.52 and 103.27% for the detection of single pesticide residues of the four OP pesticides in purple-clayed paddy soil. Moreover, the chosen ELISA was then applied to the detection of mixtures of parathion and methyl-parathion in soil samples. The average recovery and coefficient of variation were 80.91 and 4.82%, respectively. Results proved that this broad-selective ELISA would be useful for the multiresidue determination of OP pesticides. |
| doi_str_mv | 10.1021/jf904575k |
| format | Article |
| fullrecord | <record><control><sourceid>acs_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1021_jf904575k</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>b049628427</sourcerecordid><originalsourceid>FETCH-LOGICAL-a368t-8fbb2662bb8ba1ff893c8a466d0722e493e8daf6171e65f9c6053c8b054911203</originalsourceid><addsrcrecordid>eNpt0MFu1DAQBmALgehSOPAC4AsHDoEZx3acI22BVioqUuk5miTjbZYkXtnZir49Xm1pLxysOfibX6NfiLcInxAUft74GrSpzO9nYoVGQWEQ3XOxgvxZOGPxSLxKaQMAzlTwUhwpUHXlUK_EnzO-4zFsJ54XGbwk-YPa4oQS9_KcF45hDOuwS_JimnZzoJToXvoQ5XLL8iQG6otrHrlbhjuWZ_uFaZhpGcK8T7uKa5rD9jak_GJO-clpGbqh5_RavPA0Jn7zMI_Fzbevv07Pi8ur7xenXy4LKq1bCufbVlmr2ta1hN67uuwcaWt7qJRiXZfsevIWK2RrfN1ZMFm0YHSNqKA8Fh8PuV0MKUX2zTYOE8X7BqHZt9c8tpftu4Pd7tqJ-0f5r64MPjwASh2NPtLcDenJqcpqravs3h-cp9DQOmZzc60AS0CnS9TwlERdajZhF-dcwn9O-gtqy43q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Development of a Mab-Based Heterologous Immunoassay for the Broad-Selective Determination of Organophosphorus Pesticides</title><source>MEDLINE</source><source>ACS Publications</source><creator>Wang, Chunmei ; Li, Xianbin ; Liu, Yihua ; Guo, Yirong ; Xie, Rong ; Gui, Wenjun ; Zhu, Guonian</creator><creatorcontrib>Wang, Chunmei ; Li, Xianbin ; Liu, Yihua ; Guo, Yirong ; Xie, Rong ; Gui, Wenjun ; Zhu, Guonian</creatorcontrib><description>A broad-selective monoclonal antibody (Mab) for organophosphorus (OP) pesticides was raised using heterologous indirect enzyme-linked immunosorbent assay (ELISA) to screen hybridomas. On the basis of this Mab, five coating antigens were used to develop homologous and heterologous indirect competitive ELISAs. With the most suitable competitor, a sensitive and broad-selective ELISA was developed. The IC50 values were estimated to be 20.32 ng/mL for parathion, 21.44 ng/mL for methyl-parathion, 42.15 ng/mL for fenitrothion, and 58.85 ng/mL for isocarbophos. Spike recoveries were between 70.52 and 103.27% for the detection of single pesticide residues of the four OP pesticides in purple-clayed paddy soil. Moreover, the chosen ELISA was then applied to the detection of mixtures of parathion and methyl-parathion in soil samples. The average recovery and coefficient of variation were 80.91 and 4.82%, respectively. Results proved that this broad-selective ELISA would be useful for the multiresidue determination of OP pesticides.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf904575k</identifier><identifier>PMID: 20297814</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Antibodies, Monoclonal - immunology ; Biological and medical sciences ; detection ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Fish and seafood industries ; Food Chemistry/Biochemistry ; Food industries ; Fundamental and applied biological sciences. Psychology ; heterologous immunoassay ; hybridomas ; immunoassays ; Magnetic Resonance Spectroscopy ; Mice ; Mice, Inbred BALB C ; monoclonal antibodies ; Organophosphorus Compounds - analysis ; Organophosphorus Compounds - immunology ; organophosphorus pesticides ; pesticide residues ; Pesticides - analysis ; Pesticides - immunology ; pollution ; product development</subject><ispartof>Journal of agricultural and food chemistry, 2010-05, Vol.58 (9), p.5658-5663</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a368t-8fbb2662bb8ba1ff893c8a466d0722e493e8daf6171e65f9c6053c8b054911203</citedby><cites>FETCH-LOGICAL-a368t-8fbb2662bb8ba1ff893c8a466d0722e493e8daf6171e65f9c6053c8b054911203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf904575k$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf904575k$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27074,27922,27923,56736,56786</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22764447$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20297814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Chunmei</creatorcontrib><creatorcontrib>Li, Xianbin</creatorcontrib><creatorcontrib>Liu, Yihua</creatorcontrib><creatorcontrib>Guo, Yirong</creatorcontrib><creatorcontrib>Xie, Rong</creatorcontrib><creatorcontrib>Gui, Wenjun</creatorcontrib><creatorcontrib>Zhu, Guonian</creatorcontrib><title>Development of a Mab-Based Heterologous Immunoassay for the Broad-Selective Determination of Organophosphorus Pesticides</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>A broad-selective monoclonal antibody (Mab) for organophosphorus (OP) pesticides was raised using heterologous indirect enzyme-linked immunosorbent assay (ELISA) to screen hybridomas. On the basis of this Mab, five coating antigens were used to develop homologous and heterologous indirect competitive ELISAs. With the most suitable competitor, a sensitive and broad-selective ELISA was developed. The IC50 values were estimated to be 20.32 ng/mL for parathion, 21.44 ng/mL for methyl-parathion, 42.15 ng/mL for fenitrothion, and 58.85 ng/mL for isocarbophos. Spike recoveries were between 70.52 and 103.27% for the detection of single pesticide residues of the four OP pesticides in purple-clayed paddy soil. Moreover, the chosen ELISA was then applied to the detection of mixtures of parathion and methyl-parathion in soil samples. The average recovery and coefficient of variation were 80.91 and 4.82%, respectively. Results proved that this broad-selective ELISA would be useful for the multiresidue determination of OP pesticides.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Biological and medical sciences</subject><subject>detection</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>Fish and seafood industries</subject><subject>Food Chemistry/Biochemistry</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>heterologous immunoassay</subject><subject>hybridomas</subject><subject>immunoassays</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>monoclonal antibodies</subject><subject>Organophosphorus Compounds - analysis</subject><subject>Organophosphorus Compounds - immunology</subject><subject>organophosphorus pesticides</subject><subject>pesticide residues</subject><subject>Pesticides - analysis</subject><subject>Pesticides - immunology</subject><subject>pollution</subject><subject>product development</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0MFu1DAQBmALgehSOPAC4AsHDoEZx3acI22BVioqUuk5miTjbZYkXtnZir49Xm1pLxysOfibX6NfiLcInxAUft74GrSpzO9nYoVGQWEQ3XOxgvxZOGPxSLxKaQMAzlTwUhwpUHXlUK_EnzO-4zFsJ54XGbwk-YPa4oQS9_KcF45hDOuwS_JimnZzoJToXvoQ5XLL8iQG6otrHrlbhjuWZ_uFaZhpGcK8T7uKa5rD9jak_GJO-clpGbqh5_RavPA0Jn7zMI_Fzbevv07Pi8ur7xenXy4LKq1bCufbVlmr2ta1hN67uuwcaWt7qJRiXZfsevIWK2RrfN1ZMFm0YHSNqKA8Fh8PuV0MKUX2zTYOE8X7BqHZt9c8tpftu4Pd7tqJ-0f5r64MPjwASh2NPtLcDenJqcpqravs3h-cp9DQOmZzc60AS0CnS9TwlERdajZhF-dcwn9O-gtqy43q</recordid><startdate>20100512</startdate><enddate>20100512</enddate><creator>Wang, Chunmei</creator><creator>Li, Xianbin</creator><creator>Liu, Yihua</creator><creator>Guo, Yirong</creator><creator>Xie, Rong</creator><creator>Gui, Wenjun</creator><creator>Zhu, Guonian</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20100512</creationdate><title>Development of a Mab-Based Heterologous Immunoassay for the Broad-Selective Determination of Organophosphorus Pesticides</title><author>Wang, Chunmei ; Li, Xianbin ; Liu, Yihua ; Guo, Yirong ; Xie, Rong ; Gui, Wenjun ; Zhu, Guonian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a368t-8fbb2662bb8ba1ff893c8a466d0722e493e8daf6171e65f9c6053c8b054911203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Biological and medical sciences</topic><topic>detection</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Fish and seafood industries</topic><topic>Food Chemistry/Biochemistry</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>heterologous immunoassay</topic><topic>hybridomas</topic><topic>immunoassays</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>monoclonal antibodies</topic><topic>Organophosphorus Compounds - analysis</topic><topic>Organophosphorus Compounds - immunology</topic><topic>organophosphorus pesticides</topic><topic>pesticide residues</topic><topic>Pesticides - analysis</topic><topic>Pesticides - immunology</topic><topic>pollution</topic><topic>product development</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Chunmei</creatorcontrib><creatorcontrib>Li, Xianbin</creatorcontrib><creatorcontrib>Liu, Yihua</creatorcontrib><creatorcontrib>Guo, Yirong</creatorcontrib><creatorcontrib>Xie, Rong</creatorcontrib><creatorcontrib>Gui, Wenjun</creatorcontrib><creatorcontrib>Zhu, Guonian</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Chunmei</au><au>Li, Xianbin</au><au>Liu, Yihua</au><au>Guo, Yirong</au><au>Xie, Rong</au><au>Gui, Wenjun</au><au>Zhu, Guonian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a Mab-Based Heterologous Immunoassay for the Broad-Selective Determination of Organophosphorus Pesticides</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2010-05-12</date><risdate>2010</risdate><volume>58</volume><issue>9</issue><spage>5658</spage><epage>5663</epage><pages>5658-5663</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>A broad-selective monoclonal antibody (Mab) for organophosphorus (OP) pesticides was raised using heterologous indirect enzyme-linked immunosorbent assay (ELISA) to screen hybridomas. On the basis of this Mab, five coating antigens were used to develop homologous and heterologous indirect competitive ELISAs. With the most suitable competitor, a sensitive and broad-selective ELISA was developed. The IC50 values were estimated to be 20.32 ng/mL for parathion, 21.44 ng/mL for methyl-parathion, 42.15 ng/mL for fenitrothion, and 58.85 ng/mL for isocarbophos. Spike recoveries were between 70.52 and 103.27% for the detection of single pesticide residues of the four OP pesticides in purple-clayed paddy soil. Moreover, the chosen ELISA was then applied to the detection of mixtures of parathion and methyl-parathion in soil samples. The average recovery and coefficient of variation were 80.91 and 4.82%, respectively. Results proved that this broad-selective ELISA would be useful for the multiresidue determination of OP pesticides.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>20297814</pmid><doi>10.1021/jf904575k</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 2010-05, Vol.58 (9), p.5658-5663 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_crossref_primary_10_1021_jf904575k |
| source | MEDLINE; ACS Publications |
| subjects | Animals Antibodies, Monoclonal - immunology Biological and medical sciences detection Enzyme-Linked Immunosorbent Assay - methods Female Fish and seafood industries Food Chemistry/Biochemistry Food industries Fundamental and applied biological sciences. Psychology heterologous immunoassay hybridomas immunoassays Magnetic Resonance Spectroscopy Mice Mice, Inbred BALB C monoclonal antibodies Organophosphorus Compounds - analysis Organophosphorus Compounds - immunology organophosphorus pesticides pesticide residues Pesticides - analysis Pesticides - immunology pollution product development |
| title | Development of a Mab-Based Heterologous Immunoassay for the Broad-Selective Determination of Organophosphorus Pesticides |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-13T15%3A39%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-acs_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20Mab-Based%20Heterologous%20Immunoassay%20for%20the%20Broad-Selective%20Determination%20of%20Organophosphorus%20Pesticides&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Wang,%20Chunmei&rft.date=2010-05-12&rft.volume=58&rft.issue=9&rft.spage=5658&rft.epage=5663&rft.pages=5658-5663&rft.issn=0021-8561&rft.eissn=1520-5118&rft.coden=JAFCAU&rft_id=info:doi/10.1021/jf904575k&rft_dat=%3Cacs_cross%3Eb049628427%3C/acs_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/20297814&rfr_iscdi=true |