Development of an Enzyme-Linked Immunosorbent Assay for Residue Analysis of the Insecticide Emamectin Benzoate in Agricultural Products
A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) for the analysis of emamectin residues in agricultural products was developed using a prepared mouse monoclonal antibody. The working range was 0.3−3.0 ng/mL, and the 50% inhibition concentration (IC50) was 1.0 ng/mL. The assay was su...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2009-01, Vol.57 (2), p.359-364 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Kondo, Mika Yamashita, Hiroshi Uchigashima, Mikiko Kono, Takeshi Takemoto, Toshihide Fujita, Masahiro Saka, Machiko Iwasa, Seiji Ito, Shigekazu Miyake, Shiro |
| description | A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) for the analysis of emamectin residues in agricultural products was developed using a prepared mouse monoclonal antibody. The working range was 0.3−3.0 ng/mL, and the 50% inhibition concentration (IC50) was 1.0 ng/mL. The assay was sufficiently sensitive for analysis of the maximum residue limits in agricultural products in Japan (>0.1 μg/g). Emamectin residues contain the following metabolites: the 4′′-epi-amino analogue, the 4′′-epi-(N-formyl)amino analogue, the 4′′-epi-(N-formyl-N-methyl)amino analogue, and the 8,9-Z isomer. The dc-ELISA reacted with these compounds at ratios of 113, 55, 38, and 9.1% of the IC50 value of emamectin benzoate. Seven kinds of vegetables were spiked with emamectin benzoate at concentrations of 15−300 ng/g, and the recoveries were 91−117% in the dc-ELISA. The dc-ELISA results agreed reasonably well with results obtained by liquid chromatography−tandem mass spectrometry (LC-MS/MS) using spiked samples and actual (incurred) samples. The results indicate that the dc-ELISA was useful for the analysis of emamectin benzoate residues in agricultural products. |
| doi_str_mv | 10.1021/jf802788n |
| format | Article |
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The working range was 0.3−3.0 ng/mL, and the 50% inhibition concentration (IC50) was 1.0 ng/mL. The assay was sufficiently sensitive for analysis of the maximum residue limits in agricultural products in Japan (>0.1 μg/g). Emamectin residues contain the following metabolites: the 4′′-epi-amino analogue, the 4′′-epi-(N-formyl)amino analogue, the 4′′-epi-(N-formyl-N-methyl)amino analogue, and the 8,9-Z isomer. The dc-ELISA reacted with these compounds at ratios of 113, 55, 38, and 9.1% of the IC50 value of emamectin benzoate. Seven kinds of vegetables were spiked with emamectin benzoate at concentrations of 15−300 ng/g, and the recoveries were 91−117% in the dc-ELISA. The dc-ELISA results agreed reasonably well with results obtained by liquid chromatography−tandem mass spectrometry (LC-MS/MS) using spiked samples and actual (incurred) samples. The results indicate that the dc-ELISA was useful for the analysis of emamectin benzoate residues in agricultural products.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf802788n</identifier><identifier>PMID: 19117397</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytical Methods ; Animals ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Food Contamination ; Food industries ; Fundamental and applied biological sciences. Psychology ; Insecticides - analysis ; Ivermectin - analogs & derivatives ; Ivermectin - analysis ; Mice ; Mice, Inbred BALB C ; Pesticide Residues - analysis ; Vegetables - chemistry</subject><ispartof>Journal of agricultural and food chemistry, 2009-01, Vol.57 (2), p.359-364</ispartof><rights>Copyright © 2009 American Chemical Society</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a343t-8f8f29afa5b3e7a13d66c46db8784b9b22871609fb0e88bb7f68dfa593e2282e3</citedby><cites>FETCH-LOGICAL-a343t-8f8f29afa5b3e7a13d66c46db8784b9b22871609fb0e88bb7f68dfa593e2282e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf802788n$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf802788n$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21066640$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19117397$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kondo, Mika</creatorcontrib><creatorcontrib>Yamashita, Hiroshi</creatorcontrib><creatorcontrib>Uchigashima, Mikiko</creatorcontrib><creatorcontrib>Kono, Takeshi</creatorcontrib><creatorcontrib>Takemoto, Toshihide</creatorcontrib><creatorcontrib>Fujita, Masahiro</creatorcontrib><creatorcontrib>Saka, Machiko</creatorcontrib><creatorcontrib>Iwasa, Seiji</creatorcontrib><creatorcontrib>Ito, Shigekazu</creatorcontrib><creatorcontrib>Miyake, Shiro</creatorcontrib><title>Development of an Enzyme-Linked Immunosorbent Assay for Residue Analysis of the Insecticide Emamectin Benzoate in Agricultural Products</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) for the analysis of emamectin residues in agricultural products was developed using a prepared mouse monoclonal antibody. The working range was 0.3−3.0 ng/mL, and the 50% inhibition concentration (IC50) was 1.0 ng/mL. The assay was sufficiently sensitive for analysis of the maximum residue limits in agricultural products in Japan (>0.1 μg/g). Emamectin residues contain the following metabolites: the 4′′-epi-amino analogue, the 4′′-epi-(N-formyl)amino analogue, the 4′′-epi-(N-formyl-N-methyl)amino analogue, and the 8,9-Z isomer. The dc-ELISA reacted with these compounds at ratios of 113, 55, 38, and 9.1% of the IC50 value of emamectin benzoate. Seven kinds of vegetables were spiked with emamectin benzoate at concentrations of 15−300 ng/g, and the recoveries were 91−117% in the dc-ELISA. The dc-ELISA results agreed reasonably well with results obtained by liquid chromatography−tandem mass spectrometry (LC-MS/MS) using spiked samples and actual (incurred) samples. The results indicate that the dc-ELISA was useful for the analysis of emamectin benzoate residues in agricultural products.</description><subject>Analytical Methods</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Female</subject><subject>Food Contamination</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Insecticides - analysis</subject><subject>Ivermectin - analogs & derivatives</subject><subject>Ivermectin - analysis</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Pesticide Residues - analysis</subject><subject>Vegetables - chemistry</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0LFOwzAQBmALgaAUBl4AeWFgCNhJ4zhjKQUqVQIhmCPbOUNKYle-BCl9AV6bVFSwMPms--5O-gk54-yKs5hfr6xkcSal2yMjnsYsSjmX-2TEhmYkU8GPyDHiijEm04wdkiOec54leTYiX7fwCbVfN-Ba6i1Vjs7dpm8gWlbuA0q6aJrOefRBb8UUUfXU-kCfAauyAzp1qu6xwu1w-w504RBMW5mqBDpvVLP9OHoDbuNVC3Sop2-hMl3ddkHV9Cn4sjMtnpADq2qE0907Jq9385fZQ7R8vF_MpstIJZOkjaSVNs6VValOIFM8KYUwE1FqmcmJznUcy4wLllvNQEqtMytkOeg8gaEVQzImlz97TfCIAWyxDlWjQl9wVmzDLH7DHOz5j113uoHyT-7SG8DFDig0qrZBOVPhr4s5E0JM2J9TBouV78KQGf5z8Bu1FYrd</recordid><startdate>20090128</startdate><enddate>20090128</enddate><creator>Kondo, Mika</creator><creator>Yamashita, Hiroshi</creator><creator>Uchigashima, Mikiko</creator><creator>Kono, Takeshi</creator><creator>Takemoto, Toshihide</creator><creator>Fujita, Masahiro</creator><creator>Saka, Machiko</creator><creator>Iwasa, Seiji</creator><creator>Ito, Shigekazu</creator><creator>Miyake, Shiro</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20090128</creationdate><title>Development of an Enzyme-Linked Immunosorbent Assay for Residue Analysis of the Insecticide Emamectin Benzoate in Agricultural Products</title><author>Kondo, Mika ; Yamashita, Hiroshi ; Uchigashima, Mikiko ; Kono, Takeshi ; Takemoto, Toshihide ; Fujita, Masahiro ; Saka, Machiko ; Iwasa, Seiji ; Ito, Shigekazu ; Miyake, Shiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a343t-8f8f29afa5b3e7a13d66c46db8784b9b22871609fb0e88bb7f68dfa593e2282e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Analytical Methods</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Female</topic><topic>Food Contamination</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Insecticides - analysis</topic><topic>Ivermectin - analogs & derivatives</topic><topic>Ivermectin - analysis</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Pesticide Residues - analysis</topic><topic>Vegetables - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kondo, Mika</creatorcontrib><creatorcontrib>Yamashita, Hiroshi</creatorcontrib><creatorcontrib>Uchigashima, Mikiko</creatorcontrib><creatorcontrib>Kono, Takeshi</creatorcontrib><creatorcontrib>Takemoto, Toshihide</creatorcontrib><creatorcontrib>Fujita, Masahiro</creatorcontrib><creatorcontrib>Saka, Machiko</creatorcontrib><creatorcontrib>Iwasa, Seiji</creatorcontrib><creatorcontrib>Ito, Shigekazu</creatorcontrib><creatorcontrib>Miyake, Shiro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kondo, Mika</au><au>Yamashita, Hiroshi</au><au>Uchigashima, Mikiko</au><au>Kono, Takeshi</au><au>Takemoto, Toshihide</au><au>Fujita, Masahiro</au><au>Saka, Machiko</au><au>Iwasa, Seiji</au><au>Ito, Shigekazu</au><au>Miyake, Shiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of an Enzyme-Linked Immunosorbent Assay for Residue Analysis of the Insecticide Emamectin Benzoate in Agricultural Products</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2009-01-28</date><risdate>2009</risdate><volume>57</volume><issue>2</issue><spage>359</spage><epage>364</epage><pages>359-364</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) for the analysis of emamectin residues in agricultural products was developed using a prepared mouse monoclonal antibody. The working range was 0.3−3.0 ng/mL, and the 50% inhibition concentration (IC50) was 1.0 ng/mL. The assay was sufficiently sensitive for analysis of the maximum residue limits in agricultural products in Japan (>0.1 μg/g). Emamectin residues contain the following metabolites: the 4′′-epi-amino analogue, the 4′′-epi-(N-formyl)amino analogue, the 4′′-epi-(N-formyl-N-methyl)amino analogue, and the 8,9-Z isomer. The dc-ELISA reacted with these compounds at ratios of 113, 55, 38, and 9.1% of the IC50 value of emamectin benzoate. Seven kinds of vegetables were spiked with emamectin benzoate at concentrations of 15−300 ng/g, and the recoveries were 91−117% in the dc-ELISA. The dc-ELISA results agreed reasonably well with results obtained by liquid chromatography−tandem mass spectrometry (LC-MS/MS) using spiked samples and actual (incurred) samples. The results indicate that the dc-ELISA was useful for the analysis of emamectin benzoate residues in agricultural products.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>19117397</pmid><doi>10.1021/jf802788n</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2009-01, Vol.57 (2), p.359-364 |
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| source | MEDLINE; ACS Publications |
| subjects | Analytical Methods Animals Biological and medical sciences Enzyme-Linked Immunosorbent Assay - methods Female Food Contamination Food industries Fundamental and applied biological sciences. Psychology Insecticides - analysis Ivermectin - analogs & derivatives Ivermectin - analysis Mice Mice, Inbred BALB C Pesticide Residues - analysis Vegetables - chemistry |
| title | Development of an Enzyme-Linked Immunosorbent Assay for Residue Analysis of the Insecticide Emamectin Benzoate in Agricultural Products |
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