Production of Monoclonal Antibody for the Detection of Meat and Bone Meal in Animal Feed
For the detection of prohibited meat and bone meal (MBM) in animal feed, monoclonal antibodies (MAbs) were raised against heat-stable h-caldesmon purified from bovine intestinal smooth muscle. The obtained hybridoma cells were screened against extracts of the bovine MBM and heat-treated smooth muscl...
Gespeichert in:
| Veröffentlicht in: | Journal of agricultural and food chemistry 2004-12, Vol.52 (25), p.7580-7585 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7585 |
|---|---|
| container_issue | 25 |
| container_start_page | 7580 |
| container_title | Journal of agricultural and food chemistry |
| container_volume | 52 |
| creator | Kim, Shin-Hee Huang, Tung-Shi Seymour, Thomas A Wei, Cheng-i Kempf, Stephen C Bridgman, C. Roger Clemens, Roger A An, Haejung |
| description | For the detection of prohibited meat and bone meal (MBM) in animal feed, monoclonal antibodies (MAbs) were raised against heat-stable h-caldesmon purified from bovine intestinal smooth muscle. The obtained hybridoma cells were screened against extracts of the bovine MBM and heat-treated smooth muscle, and MAb 5E12 was identified as having the best performance. Antibody 5E12 did not react with animal feed, milk product, plant proteins, and other ingredients used for commercial animal feed except for the gelatin. This antibody diluted to 100-fold was able to detect MBM mixed in animal feed at 0.05% in an ELISA, and it showed strong affinity toward bovine smooth muscle autoclaved at 130 °C. Therefore, this antibody can be used in the ELISA system for field testing of the presence of MBM in animal feed. Keywords: Meat and bone meal; animal feed; monoclonal antibody; ELISA; immunoassay |
| doi_str_mv | 10.1021/jf048789a |
| format | Article |
| fullrecord | <record><control><sourceid>acs_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1021_jf048789a</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>e33509252</sourcerecordid><originalsourceid>FETCH-LOGICAL-a335t-2a65cb796b56615ea6ee976269610ea1aeb6d845efd189faf9ddd63a907d59c93</originalsourceid><addsrcrecordid>eNpt0c1uEzEQAGALgWgoHHgB8IVDDwvj3fjv2JYGkIKIlFRws2bXY9iwXVf2RqJvj6NEDQdOtjWfZ8Zjxl4LeC-gFh-2AeZGG4tP2EzIGiophHnKZlCClZFKnLEXOW8BwEgNz9mZkEpLA3rGfqxS9Ltu6uPIY-Bf4xi7IY448Mtx6tvoH3iIiU-_iH-kiU6QcOI4en4VR9qfBt6P5U5_V3YLIv-SPQs4ZHp1XM_Z7eJmc_25Wn779OX6cllh08ipqlHJrtVWtVIpIQkVkdWqVlYJIBRIrfJmLil4YWzAYL33qkEL2kvb2eacXRzydinmnCi4-1SaSA9OgNtPxz1Op9g3B3u_a-_In-RxHAW8OwLMHQ4h4dj1-eRU0xgL-6LVwfV5oj-PcUy_ndKNlm6zWrvFar78vhEbty7-7cEHjA5_ppLzdl2DaACsUnb-T2XsstvGXSpfkP_zhL-1TZA9</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Production of Monoclonal Antibody for the Detection of Meat and Bone Meal in Animal Feed</title><source>MEDLINE</source><source>ACS Publications</source><creator>Kim, Shin-Hee ; Huang, Tung-Shi ; Seymour, Thomas A ; Wei, Cheng-i ; Kempf, Stephen C ; Bridgman, C. Roger ; Clemens, Roger A ; An, Haejung</creator><creatorcontrib>Kim, Shin-Hee ; Huang, Tung-Shi ; Seymour, Thomas A ; Wei, Cheng-i ; Kempf, Stephen C ; Bridgman, C. Roger ; Clemens, Roger A ; An, Haejung</creatorcontrib><description>For the detection of prohibited meat and bone meal (MBM) in animal feed, monoclonal antibodies (MAbs) were raised against heat-stable h-caldesmon purified from bovine intestinal smooth muscle. The obtained hybridoma cells were screened against extracts of the bovine MBM and heat-treated smooth muscle, and MAb 5E12 was identified as having the best performance. Antibody 5E12 did not react with animal feed, milk product, plant proteins, and other ingredients used for commercial animal feed except for the gelatin. This antibody diluted to 100-fold was able to detect MBM mixed in animal feed at 0.05% in an ELISA, and it showed strong affinity toward bovine smooth muscle autoclaved at 130 °C. Therefore, this antibody can be used in the ELISA system for field testing of the presence of MBM in animal feed. Keywords: Meat and bone meal; animal feed; monoclonal antibody; ELISA; immunoassay</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf048789a</identifier><identifier>PMID: 15675807</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animal Feed - analysis ; animal proteins ; Animals ; Antibodies, Monoclonal - biosynthesis ; Antibody Specificity ; Antigens - immunology ; Biological and medical sciences ; Biological Products ; Cattle ; Enzyme-Linked Immunosorbent Assay ; Feed and pet food industries ; feed contamination ; feeds ; Food Contamination - analysis ; Food industries ; Fundamental and applied biological sciences. Psychology ; General aspects ; h-caldesmon ; Hot Temperature ; Hybridomas - immunology ; immunohistochemistry ; Intestines - immunology ; Meat - analysis ; meat and bone meal ; Methods of analysis, processing and quality control, regulation, standards ; Mice ; Minerals - analysis ; monoclonal antibodies ; Muscle, Smooth - immunology ; processing residues ; smooth muscle</subject><ispartof>Journal of agricultural and food chemistry, 2004-12, Vol.52 (25), p.7580-7585</ispartof><rights>Copyright © 2004 American Chemical Society</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a335t-2a65cb796b56615ea6ee976269610ea1aeb6d845efd189faf9ddd63a907d59c93</citedby><cites>FETCH-LOGICAL-a335t-2a65cb796b56615ea6ee976269610ea1aeb6d845efd189faf9ddd63a907d59c93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf048789a$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf048789a$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16338909$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15675807$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Shin-Hee</creatorcontrib><creatorcontrib>Huang, Tung-Shi</creatorcontrib><creatorcontrib>Seymour, Thomas A</creatorcontrib><creatorcontrib>Wei, Cheng-i</creatorcontrib><creatorcontrib>Kempf, Stephen C</creatorcontrib><creatorcontrib>Bridgman, C. Roger</creatorcontrib><creatorcontrib>Clemens, Roger A</creatorcontrib><creatorcontrib>An, Haejung</creatorcontrib><title>Production of Monoclonal Antibody for the Detection of Meat and Bone Meal in Animal Feed</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>For the detection of prohibited meat and bone meal (MBM) in animal feed, monoclonal antibodies (MAbs) were raised against heat-stable h-caldesmon purified from bovine intestinal smooth muscle. The obtained hybridoma cells were screened against extracts of the bovine MBM and heat-treated smooth muscle, and MAb 5E12 was identified as having the best performance. Antibody 5E12 did not react with animal feed, milk product, plant proteins, and other ingredients used for commercial animal feed except for the gelatin. This antibody diluted to 100-fold was able to detect MBM mixed in animal feed at 0.05% in an ELISA, and it showed strong affinity toward bovine smooth muscle autoclaved at 130 °C. Therefore, this antibody can be used in the ELISA system for field testing of the presence of MBM in animal feed. Keywords: Meat and bone meal; animal feed; monoclonal antibody; ELISA; immunoassay</description><subject>Animal Feed - analysis</subject><subject>animal proteins</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibody Specificity</subject><subject>Antigens - immunology</subject><subject>Biological and medical sciences</subject><subject>Biological Products</subject><subject>Cattle</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Feed and pet food industries</subject><subject>feed contamination</subject><subject>feeds</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>h-caldesmon</subject><subject>Hot Temperature</subject><subject>Hybridomas - immunology</subject><subject>immunohistochemistry</subject><subject>Intestines - immunology</subject><subject>Meat - analysis</subject><subject>meat and bone meal</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>Mice</subject><subject>Minerals - analysis</subject><subject>monoclonal antibodies</subject><subject>Muscle, Smooth - immunology</subject><subject>processing residues</subject><subject>smooth muscle</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0c1uEzEQAGALgWgoHHgB8IVDDwvj3fjv2JYGkIKIlFRws2bXY9iwXVf2RqJvj6NEDQdOtjWfZ8Zjxl4LeC-gFh-2AeZGG4tP2EzIGiophHnKZlCClZFKnLEXOW8BwEgNz9mZkEpLA3rGfqxS9Ltu6uPIY-Bf4xi7IY448Mtx6tvoH3iIiU-_iH-kiU6QcOI4en4VR9qfBt6P5U5_V3YLIv-SPQs4ZHp1XM_Z7eJmc_25Wn779OX6cllh08ipqlHJrtVWtVIpIQkVkdWqVlYJIBRIrfJmLil4YWzAYL33qkEL2kvb2eacXRzydinmnCi4-1SaSA9OgNtPxz1Op9g3B3u_a-_In-RxHAW8OwLMHQ4h4dj1-eRU0xgL-6LVwfV5oj-PcUy_ndKNlm6zWrvFar78vhEbty7-7cEHjA5_ppLzdl2DaACsUnb-T2XsstvGXSpfkP_zhL-1TZA9</recordid><startdate>20041215</startdate><enddate>20041215</enddate><creator>Kim, Shin-Hee</creator><creator>Huang, Tung-Shi</creator><creator>Seymour, Thomas A</creator><creator>Wei, Cheng-i</creator><creator>Kempf, Stephen C</creator><creator>Bridgman, C. Roger</creator><creator>Clemens, Roger A</creator><creator>An, Haejung</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20041215</creationdate><title>Production of Monoclonal Antibody for the Detection of Meat and Bone Meal in Animal Feed</title><author>Kim, Shin-Hee ; Huang, Tung-Shi ; Seymour, Thomas A ; Wei, Cheng-i ; Kempf, Stephen C ; Bridgman, C. Roger ; Clemens, Roger A ; An, Haejung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a335t-2a65cb796b56615ea6ee976269610ea1aeb6d845efd189faf9ddd63a907d59c93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animal Feed - analysis</topic><topic>animal proteins</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibody Specificity</topic><topic>Antigens - immunology</topic><topic>Biological and medical sciences</topic><topic>Biological Products</topic><topic>Cattle</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Feed and pet food industries</topic><topic>feed contamination</topic><topic>feeds</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>h-caldesmon</topic><topic>Hot Temperature</topic><topic>Hybridomas - immunology</topic><topic>immunohistochemistry</topic><topic>Intestines - immunology</topic><topic>Meat - analysis</topic><topic>meat and bone meal</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>Mice</topic><topic>Minerals - analysis</topic><topic>monoclonal antibodies</topic><topic>Muscle, Smooth - immunology</topic><topic>processing residues</topic><topic>smooth muscle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Shin-Hee</creatorcontrib><creatorcontrib>Huang, Tung-Shi</creatorcontrib><creatorcontrib>Seymour, Thomas A</creatorcontrib><creatorcontrib>Wei, Cheng-i</creatorcontrib><creatorcontrib>Kempf, Stephen C</creatorcontrib><creatorcontrib>Bridgman, C. Roger</creatorcontrib><creatorcontrib>Clemens, Roger A</creatorcontrib><creatorcontrib>An, Haejung</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Shin-Hee</au><au>Huang, Tung-Shi</au><au>Seymour, Thomas A</au><au>Wei, Cheng-i</au><au>Kempf, Stephen C</au><au>Bridgman, C. Roger</au><au>Clemens, Roger A</au><au>An, Haejung</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of Monoclonal Antibody for the Detection of Meat and Bone Meal in Animal Feed</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2004-12-15</date><risdate>2004</risdate><volume>52</volume><issue>25</issue><spage>7580</spage><epage>7585</epage><pages>7580-7585</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>For the detection of prohibited meat and bone meal (MBM) in animal feed, monoclonal antibodies (MAbs) were raised against heat-stable h-caldesmon purified from bovine intestinal smooth muscle. The obtained hybridoma cells were screened against extracts of the bovine MBM and heat-treated smooth muscle, and MAb 5E12 was identified as having the best performance. Antibody 5E12 did not react with animal feed, milk product, plant proteins, and other ingredients used for commercial animal feed except for the gelatin. This antibody diluted to 100-fold was able to detect MBM mixed in animal feed at 0.05% in an ELISA, and it showed strong affinity toward bovine smooth muscle autoclaved at 130 °C. Therefore, this antibody can be used in the ELISA system for field testing of the presence of MBM in animal feed. Keywords: Meat and bone meal; animal feed; monoclonal antibody; ELISA; immunoassay</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>15675807</pmid><doi>10.1021/jf048789a</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-8561 |
| ispartof | Journal of agricultural and food chemistry, 2004-12, Vol.52 (25), p.7580-7585 |
| issn | 0021-8561 1520-5118 |
| language | eng |
| recordid | cdi_crossref_primary_10_1021_jf048789a |
| source | MEDLINE; ACS Publications |
| subjects | Animal Feed - analysis animal proteins Animals Antibodies, Monoclonal - biosynthesis Antibody Specificity Antigens - immunology Biological and medical sciences Biological Products Cattle Enzyme-Linked Immunosorbent Assay Feed and pet food industries feed contamination feeds Food Contamination - analysis Food industries Fundamental and applied biological sciences. Psychology General aspects h-caldesmon Hot Temperature Hybridomas - immunology immunohistochemistry Intestines - immunology Meat - analysis meat and bone meal Methods of analysis, processing and quality control, regulation, standards Mice Minerals - analysis monoclonal antibodies Muscle, Smooth - immunology processing residues smooth muscle |
| title | Production of Monoclonal Antibody for the Detection of Meat and Bone Meal in Animal Feed |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T15%3A18%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-acs_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Production%20of%20Monoclonal%20Antibody%20for%20the%20Detection%20of%20Meat%20and%20Bone%20Meal%20in%20Animal%20Feed&rft.jtitle=Journal%20of%20agricultural%20and%20food%20chemistry&rft.au=Kim,%20Shin-Hee&rft.date=2004-12-15&rft.volume=52&rft.issue=25&rft.spage=7580&rft.epage=7585&rft.pages=7580-7585&rft.issn=0021-8561&rft.eissn=1520-5118&rft.coden=JAFCAU&rft_id=info:doi/10.1021/jf048789a&rft_dat=%3Cacs_cross%3Ee33509252%3C/acs_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/15675807&rfr_iscdi=true |