Development of a Rapid and Sensitive SPE-LC-ESI MS/MS Method for the Determination of Chloramphenicol in Seafood
A method based on liquid chromatography−tandem mass spectrometry was developed and validated for the qualitative and quantitative detection of chloramphenicol (CAP) in seafood samples. The analysis of CAP residues in seafood is important because CAP can cause serious acute reactions in humans, inclu...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2004-03, Vol.52 (5), p.1025-1030 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Gikas, Evangelos Kormali, Pigi Tsipi, Despina Tsarbopoulos, Anthony |
| description | A method based on liquid chromatography−tandem mass spectrometry was developed and validated for the qualitative and quantitative detection of chloramphenicol (CAP) in seafood samples. The analysis of CAP residues in seafood is important because CAP can cause serious acute reactions in humans, including aplastic anemia and leukemia. The proposed methodology includes a cleanup solid-phase extraction procedure with high recovery efficiency (>90%). Chromatographic separation of CAP and the internal standard (IS) was carried out on a C18 column, followed by mass spectrometric detection using electrospray ionization in the negative-ion mode. The precursor/product ion transitions 321→257 (CAP) and 354→290 (IS) were monitored. Statistical evaluation of this multiple reaction monitoring mass spectrometric procedure reveals good linearity, accuracy, and inter- and intraday precisions. The limit of detection was 0.1 ng/mL, and the limit of quantification for CAP in seafood samples is 0.02 μg/kg. Application in seafood samples allowed the detection of CAP in low parts per billion levels. Keywords: Chloramphenicol; seafood; liquid chromatography−tandem mass spectrometry; triple quadrupole mass spectrometer; solid-phase extraction |
| doi_str_mv | 10.1021/jf030485l |
| format | Article |
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The analysis of CAP residues in seafood is important because CAP can cause serious acute reactions in humans, including aplastic anemia and leukemia. The proposed methodology includes a cleanup solid-phase extraction procedure with high recovery efficiency (>90%). Chromatographic separation of CAP and the internal standard (IS) was carried out on a C18 column, followed by mass spectrometric detection using electrospray ionization in the negative-ion mode. The precursor/product ion transitions 321→257 (CAP) and 354→290 (IS) were monitored. Statistical evaluation of this multiple reaction monitoring mass spectrometric procedure reveals good linearity, accuracy, and inter- and intraday precisions. The limit of detection was 0.1 ng/mL, and the limit of quantification for CAP in seafood samples is 0.02 μg/kg. Application in seafood samples allowed the detection of CAP in low parts per billion levels. 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Agric. Food Chem</addtitle><description>A method based on liquid chromatography−tandem mass spectrometry was developed and validated for the qualitative and quantitative detection of chloramphenicol (CAP) in seafood samples. The analysis of CAP residues in seafood is important because CAP can cause serious acute reactions in humans, including aplastic anemia and leukemia. The proposed methodology includes a cleanup solid-phase extraction procedure with high recovery efficiency (>90%). Chromatographic separation of CAP and the internal standard (IS) was carried out on a C18 column, followed by mass spectrometric detection using electrospray ionization in the negative-ion mode. The precursor/product ion transitions 321→257 (CAP) and 354→290 (IS) were monitored. Statistical evaluation of this multiple reaction monitoring mass spectrometric procedure reveals good linearity, accuracy, and inter- and intraday precisions. The limit of detection was 0.1 ng/mL, and the limit of quantification for CAP in seafood samples is 0.02 μg/kg. Application in seafood samples allowed the detection of CAP in low parts per billion levels. Keywords: Chloramphenicol; seafood; liquid chromatography−tandem mass spectrometry; triple quadrupole mass spectrometer; solid-phase extraction</description><subject>Biological and medical sciences</subject><subject>Chloramphenicol - analysis</subject><subject>Chromatography, Liquid - methods</subject><subject>Fish and seafood industries</subject><subject>Food Contamination - analysis</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Seafood - analysis</subject><subject>Sensitivity and Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEFP2zAUgK1paBS2w_4A8mUHDoFnJ3aSI5QOEK3oSKcdLcexVZfEjuyAxr8nVSt64fQO79On9z6EfhK4IEDJ5cZAClnB2i9oQhiFhBFSfEUTGJdJwTg5RicxbgCgYDl8Q8ckK0sGJZ2g_ka_6tb3nXYD9gZL_CR722DpGlxpF-1gXzWulrNkPk1m1T1eVJeLCi_0sPYNNj7gYa3xjR506KyTg_Vuq5muWx9k16-1s8q32LrRJo33zXd0ZGQb9Y_9PEV_f89W07tk_nh7P72aJzLNyyEpoKY8TTU1YNK6znlBx0egUczwOoO6TDlRXGVUlmXNOciCZ5LQIlOK8Dxr0lN0vvOq4GMM2og-2E6GN0FAbKuJj2oje7Zj-5e6082B3GcagV97QEYlWxOkUzYeODY2prDlkh1n46D_f-xleBY8T3MmVstK_PvzcL18WpZidfBKFcXGvwQ3JvnkwHf4kY3z</recordid><startdate>20040310</startdate><enddate>20040310</enddate><creator>Gikas, Evangelos</creator><creator>Kormali, Pigi</creator><creator>Tsipi, Despina</creator><creator>Tsarbopoulos, Anthony</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20040310</creationdate><title>Development of a Rapid and Sensitive SPE-LC-ESI MS/MS Method for the Determination of Chloramphenicol in Seafood</title><author>Gikas, Evangelos ; Kormali, Pigi ; Tsipi, Despina ; Tsarbopoulos, Anthony</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a379t-80b2633e2f0f3bb76828560dc5f6b40b9361c6c42a99b660a864a1284cc1674d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Biological and medical sciences</topic><topic>Chloramphenicol - analysis</topic><topic>Chromatography, Liquid - methods</topic><topic>Fish and seafood industries</topic><topic>Food Contamination - analysis</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Seafood - analysis</topic><topic>Sensitivity and Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gikas, Evangelos</creatorcontrib><creatorcontrib>Kormali, Pigi</creatorcontrib><creatorcontrib>Tsipi, Despina</creatorcontrib><creatorcontrib>Tsarbopoulos, Anthony</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gikas, Evangelos</au><au>Kormali, Pigi</au><au>Tsipi, Despina</au><au>Tsarbopoulos, Anthony</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a Rapid and Sensitive SPE-LC-ESI MS/MS Method for the Determination of Chloramphenicol in Seafood</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2004-03-10</date><risdate>2004</risdate><volume>52</volume><issue>5</issue><spage>1025</spage><epage>1030</epage><pages>1025-1030</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>A method based on liquid chromatography−tandem mass spectrometry was developed and validated for the qualitative and quantitative detection of chloramphenicol (CAP) in seafood samples. The analysis of CAP residues in seafood is important because CAP can cause serious acute reactions in humans, including aplastic anemia and leukemia. The proposed methodology includes a cleanup solid-phase extraction procedure with high recovery efficiency (>90%). Chromatographic separation of CAP and the internal standard (IS) was carried out on a C18 column, followed by mass spectrometric detection using electrospray ionization in the negative-ion mode. The precursor/product ion transitions 321→257 (CAP) and 354→290 (IS) were monitored. Statistical evaluation of this multiple reaction monitoring mass spectrometric procedure reveals good linearity, accuracy, and inter- and intraday precisions. The limit of detection was 0.1 ng/mL, and the limit of quantification for CAP in seafood samples is 0.02 μg/kg. Application in seafood samples allowed the detection of CAP in low parts per billion levels. Keywords: Chloramphenicol; seafood; liquid chromatography−tandem mass spectrometry; triple quadrupole mass spectrometer; solid-phase extraction</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>14995092</pmid><doi>10.1021/jf030485l</doi><tpages>6</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2004-03, Vol.52 (5), p.1025-1030 |
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| language | eng |
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| source | MEDLINE; ACS Publications |
| subjects | Biological and medical sciences Chloramphenicol - analysis Chromatography, Liquid - methods Fish and seafood industries Food Contamination - analysis Food industries Fundamental and applied biological sciences. Psychology Seafood - analysis Sensitivity and Specificity Spectrometry, Mass, Electrospray Ionization |
| title | Development of a Rapid and Sensitive SPE-LC-ESI MS/MS Method for the Determination of Chloramphenicol in Seafood |
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