Metabolic Profiles of the Mycotoxin Zearalenone and of the Growth Promoter Zeranol in Urine, Liver, and Muscle of Heifers

A group of five heifers were fed for 84 days with 2 kg of zearalenone-contaminated oats (1370 μg/kg) resulting in an average daily intake of 2740 μg of zearalenone per animal. In a parallel experiment five heifers were implanted with two 25 mg zeranol pellets, one at the beginning of the study and o...

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Veröffentlicht in:Journal of agricultural and food chemistry 2002-08, Vol.50 (17), p.4769-4776
Hauptverfasser: Kleinova, Martina, Zöllner, Peter, Kahlbacher, Hermann, Hochsteiner, Werner, Lindner, Wolfgang
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container_end_page 4776
container_issue 17
container_start_page 4769
container_title Journal of agricultural and food chemistry
container_volume 50
creator Kleinova, Martina
Zöllner, Peter
Kahlbacher, Hermann
Hochsteiner, Werner
Lindner, Wolfgang
description A group of five heifers were fed for 84 days with 2 kg of zearalenone-contaminated oats (1370 μg/kg) resulting in an average daily intake of 2740 μg of zearalenone per animal. In a parallel experiment five heifers were implanted with two 25 mg zeranol pellets, one at the beginning of the study and one after 42 days, and fed with 2 kg of “blank” control oats (79 μg/kg, daily intake = 158 μg). A third group of five animals were also fed with 2 kg of “blank” oats and served as control. Urine samples of all animals were collected every 5−6 days during the whole period of the study. Animals of all three groups were killed 84 days after the beginning of the feeding study. Tissue samples (back, femoral region, liver, and residues of implanted pellets) were taken during post-mortem investigations. The content of zearalenone and zeranol and their metabolites in urine and tissue samples was established by an analytical method combining solid-phase extraction and high-performance liquid chromatography−tandem mass spectrometry. Urinary excretion rates of zeralenone and zeranol were calculated from these results. Keywords: Zearalenone; zeranol; α-zearalanol; metabolism; heifer; cow; feeding study
doi_str_mv 10.1021/jf020160p
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Agric. Food Chem</addtitle><description>A group of five heifers were fed for 84 days with 2 kg of zearalenone-contaminated oats (1370 μg/kg) resulting in an average daily intake of 2740 μg of zearalenone per animal. In a parallel experiment five heifers were implanted with two 25 mg zeranol pellets, one at the beginning of the study and one after 42 days, and fed with 2 kg of “blank” control oats (79 μg/kg, daily intake = 158 μg). A third group of five animals were also fed with 2 kg of “blank” oats and served as control. Urine samples of all animals were collected every 5−6 days during the whole period of the study. Animals of all three groups were killed 84 days after the beginning of the feeding study. Tissue samples (back, femoral region, liver, and residues of implanted pellets) were taken during post-mortem investigations. The content of zearalenone and zeranol and their metabolites in urine and tissue samples was established by an analytical method combining solid-phase extraction and high-performance liquid chromatography−tandem mass spectrometry. Urinary excretion rates of zeralenone and zeranol were calculated from these results. Keywords: Zearalenone; zeranol; α-zearalanol; metabolism; heifer; cow; feeding study</description><subject>Animals</subject><subject>Avena</subject><subject>Biological and medical sciences</subject><subject>Cattle - metabolism</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Drug Implants</subject><subject>Estrogens, Non-Steroidal - administration &amp; dosage</subject><subject>Estrogens, Non-Steroidal - pharmacokinetics</subject><subject>Estrogens, Non-Steroidal - urine</subject><subject>Female</subject><subject>Food Contamination</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Liver - chemistry</subject><subject>Liver - metabolism</subject><subject>Mass Spectrometry</subject><subject>Meat and meat product industries</subject><subject>Muscle, Skeletal - chemistry</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Zearalenone - administration &amp; dosage</subject><subject>Zearalenone - pharmacokinetics</subject><subject>Zearalenone - urine</subject><subject>Zeranol - administration &amp; dosage</subject><subject>Zeranol - pharmacokinetics</subject><subject>Zeranol - urine</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0E1PGzEQBmCrKiqB9tA_UO2lByS22N61s3tEUD7UrIjUcOnFmrXHwmGzjuwNJf8ehwRy4eTDPO_I8xLyndFfjHJ2NreUUybp8hMZMcFpLhirPpMRTcO8EpIdkqMY55TSSozpF3LIOJOyFtWIrBscoPWd09k0eOs6jJm32fCAWbPWfvDPrs_-IQTosPc9ZtCbN3Ad_P_hYZNb-AFDYgF632UpcR9cj6fZxD1hOH3NNKuoO9xEb9BZDPErObDQRfy2e4_J_dXv2cVNPrm7vr04n-RQlmLIUWtLS7BSsEqn41qujaU1ItQA2nBtoSpsa2RpamnagnMjqTSSUUQqS1Yck5PtXh18jAGtWga3gLBWjKpNfeq9vmR_bO1y1S7Q7OWurwR-7gBEDZ1NB2sX966oSlEznly-dS4O-Pw-h_Co5LgYCzWb_lXyz3TWXFYT1ez3go5q7lehT5V88MEXsn-ULQ</recordid><startdate>20020814</startdate><enddate>20020814</enddate><creator>Kleinova, Martina</creator><creator>Zöllner, Peter</creator><creator>Kahlbacher, Hermann</creator><creator>Hochsteiner, Werner</creator><creator>Lindner, Wolfgang</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20020814</creationdate><title>Metabolic Profiles of the Mycotoxin Zearalenone and of the Growth Promoter Zeranol in Urine, Liver, and Muscle of Heifers</title><author>Kleinova, Martina ; Zöllner, Peter ; Kahlbacher, Hermann ; Hochsteiner, Werner ; Lindner, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a445t-eccf04af6518c152b2cdf09eea9aacd2cfa83fbd64d96db322d606d610ee06413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Animals</topic><topic>Avena</topic><topic>Biological and medical sciences</topic><topic>Cattle - metabolism</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Drug Implants</topic><topic>Estrogens, Non-Steroidal - administration &amp; dosage</topic><topic>Estrogens, Non-Steroidal - pharmacokinetics</topic><topic>Estrogens, Non-Steroidal - urine</topic><topic>Female</topic><topic>Food Contamination</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Liver - chemistry</topic><topic>Liver - metabolism</topic><topic>Mass Spectrometry</topic><topic>Meat and meat product industries</topic><topic>Muscle, Skeletal - chemistry</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Zearalenone - administration &amp; dosage</topic><topic>Zearalenone - pharmacokinetics</topic><topic>Zearalenone - urine</topic><topic>Zeranol - administration &amp; dosage</topic><topic>Zeranol - pharmacokinetics</topic><topic>Zeranol - urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kleinova, Martina</creatorcontrib><creatorcontrib>Zöllner, Peter</creatorcontrib><creatorcontrib>Kahlbacher, Hermann</creatorcontrib><creatorcontrib>Hochsteiner, Werner</creatorcontrib><creatorcontrib>Lindner, Wolfgang</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kleinova, Martina</au><au>Zöllner, Peter</au><au>Kahlbacher, Hermann</au><au>Hochsteiner, Werner</au><au>Lindner, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic Profiles of the Mycotoxin Zearalenone and of the Growth Promoter Zeranol in Urine, Liver, and Muscle of Heifers</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2002-08-14</date><risdate>2002</risdate><volume>50</volume><issue>17</issue><spage>4769</spage><epage>4776</epage><pages>4769-4776</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>A group of five heifers were fed for 84 days with 2 kg of zearalenone-contaminated oats (1370 μg/kg) resulting in an average daily intake of 2740 μg of zearalenone per animal. In a parallel experiment five heifers were implanted with two 25 mg zeranol pellets, one at the beginning of the study and one after 42 days, and fed with 2 kg of “blank” control oats (79 μg/kg, daily intake = 158 μg). A third group of five animals were also fed with 2 kg of “blank” oats and served as control. Urine samples of all animals were collected every 5−6 days during the whole period of the study. Animals of all three groups were killed 84 days after the beginning of the feeding study. 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source ACS Publications; MEDLINE
subjects Animals
Avena
Biological and medical sciences
Cattle - metabolism
Chromatography, High Pressure Liquid
Drug Implants
Estrogens, Non-Steroidal - administration & dosage
Estrogens, Non-Steroidal - pharmacokinetics
Estrogens, Non-Steroidal - urine
Female
Food Contamination
Food industries
Fundamental and applied biological sciences. Psychology
Liver - chemistry
Liver - metabolism
Mass Spectrometry
Meat and meat product industries
Muscle, Skeletal - chemistry
Muscle, Skeletal - metabolism
Zearalenone - administration & dosage
Zearalenone - pharmacokinetics
Zearalenone - urine
Zeranol - administration & dosage
Zeranol - pharmacokinetics
Zeranol - urine
title Metabolic Profiles of the Mycotoxin Zearalenone and of the Growth Promoter Zeranol in Urine, Liver, and Muscle of Heifers
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